MD01G1162900.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003044
Physical Location & Seq
Reverse (-)
26802391 .. 26804100
1710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1162900.v1.1.491

Sequence Viewer

Length: 675 bp
ATGTCCAGAGAAAGGGAGAGATTTGATGAGGTAGGCAAGAAGTTTACAAGAGAAACTGATGCTTCCCATTATCAAATGGGAAGAAGACACATGTTGGGCCCTCCTGGAACCCTAAACACCATCACTCCTTGTGCTGCCTGTAAACTCCTCAGACGAAGGTGTTCACAGGAATGCCCCTTCTCTCCTTACTTCTCTCCCCACGAACCCCAGAAGTTTGCCTCGGTCCATAAGGTCTTTGGTGCCAGCAACGTCTCGAAGATGCTCATGGAGGTGCCGGAGACTCAAAGAGCAGATGCAGCAAATAGTCTTGTTTATGAAGCAAATGTGAGGCTAAGAGATCCGGTGTATGGATGCATGGGTGTAATTTCAGCTTTGCAACAACAAGTTCAATCTTTACAAGCTGAGCTGAACGCAGTAAGGGGCGAGATACTTAAATACAAATATAGGGAAGCAAATCTCCTTCCTTCTAATTCTCATCATCATATGGCTTTGTTCTCTACTTCCGGGGCTGTTTCCGTTGCTTCCTTGCCGCAGACCCCACAACCACCGCCGCCACCATCACCGCCACCTCCTCTACCTCCTACTTCTTCCTCTTCTTCTATGTACACGAATCAACAAACCAGTGCCGTGGACTATACCTCCCTTTCAAGTGAAAATGTTTCCTTCTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

225

Amino Acids

24.75

Weight (kDa)

8.83

Isoelectric Point (pI)

67.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 43 - 139 4.1e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 239, 271
AciI CCGC 4 cut(s) 530, 548, 551, 563
AclWI GGATC 1 cut(s) 332
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 2 cut(s) 12, 347
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 2 cut(s) 389, 648
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 3 cut(s) 371, 401, 406
AluI AGCT 3 cut(s) 371, 401, 406
Alw26I GTCTC 2 cut(s) 256, 272
AlwI GGATC 1 cut(s) 332
AoxI GGCC 1 cut(s) 97
ApaI GGGCCC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 134, 296
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 3 cut(s) 97, 98, 223
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 1 cut(s) 552
AvaII GGWCC 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 101
BanI GGYRCC 2 cut(s) 239, 271
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 91
BbvI GCAGC 2 cut(s) 121, 308
BccI CCATC 2 cut(s) 128, 565
BceAI ACGGC 1 cut(s) 611
BciT130I CCWGG 1 cut(s) 105
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 256, 272
BisI GCNGC 4 cut(s) 135, 297, 530, 551
BlpI GCTNAGC 1 cut(s) 402
BlsI GCNGC 4 cut(s) 136, 298, 531, 552
Bme1390I CCNGG 2 cut(s) 105, 505
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 97, 98, 223
BmiI GGNNCC 4 cut(s) 99, 109, 241, 273
BmrFI CCNGG 2 cut(s) 105, 505
BmsI GCATC 4 cut(s) 49, 249, 283, 341
BpiI GAAGAC 1 cut(s) 91
Bpu1102I GCTNAGC 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 219, 504, 627
BsaWI WCCGGW 1 cut(s) 340
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 623, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 347
Bse1I ACTGG 1 cut(s) 621
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 219, 504, 627
BseGI GGATG 1 cut(s) 356
BseLI CCNNNNNNNGG 2 cut(s) 12, 347
BseMII CTCAG 2 cut(s) 163, 393
BseNI ACTGG 1 cut(s) 621
BseRI GAGGAG 2 cut(s) 137, 561
BseSI GKGCMC 1 cut(s) 101
BseXI GCAGC 2 cut(s) 121, 308
BshFI GGCC 1 cut(s) 99
BshNI GGYRCC 2 cut(s) 239, 271
BsiSI CCGG 3 cut(s) 275, 341, 504
BslI CCNNNNNNNGG 2 cut(s) 12, 347
BsmAI GTCTC 2 cut(s) 256, 272
BsmBI CGTCTC 1 cut(s) 256
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 1 cut(s) 99
Bsp120I GGGCCC 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 101
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 1 cut(s) 337
Bsp1720I GCTNAGC 1 cut(s) 402
BspACI CCGC 4 cut(s) 530, 548, 551, 563
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 2 cut(s) 162, 394
BspLI GGNNCC 4 cut(s) 99, 109, 241, 273
BspPI GGATC 1 cut(s) 332
BspT107I GGYRCC 2 cut(s) 239, 271
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 1 cut(s) 621
BssECI CCNNGG 3 cut(s) 219, 504, 627
BssMI GATC 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 3 cut(s) 149, 332, 402
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 1 cut(s) 356
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 2 cut(s) 256, 272
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 103, 503
BstSLI GKGCMC 1 cut(s) 101
BstV1I GCAGC 2 cut(s) 121, 308
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 628
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 1 cut(s) 99
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 1 cut(s) 356
BtsIMutI CAGTG 1 cut(s) 628
Cac8I GCNNGC 1 cut(s) 244
Cfr13I GGNCC 3 cut(s) 97, 98, 223
Csp6I GTAC 1 cut(s) 604
CviAII CATG 3 cut(s) 91, 265, 355
CviJI RGCY 7 cut(s) 99, 331, 371, 401, 406, 488, 509
CviKI_1 RGCY 7 cut(s) 99, 331, 371, 401, 406, 488, 509
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 3 cut(s) 149, 332, 402
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 103
EcoT22I ATGCAT 1 cut(s) 356
EcoT38I GRGCYC 1 cut(s) 101
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 3 cut(s) 94, 268, 358
FatI CATG 3 cut(s) 90, 264, 354
FauNDI CATATG 1 cut(s) 483
Fnu4HI GCNGC 4 cut(s) 135, 297, 530, 551
FokI GGATG 1 cut(s) 363
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 135, 297, 530, 551
GluI GCNGC 4 cut(s) 135, 297, 530, 551
HaeIII GGCC 1 cut(s) 99
HapII CCGG 3 cut(s) 275, 341, 504
Hin1II CATG 3 cut(s) 94, 268, 358
HinfI GANTC 2 cut(s) 280, 610
HpaII CCGG 3 cut(s) 275, 341, 504
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 5 cut(s) 45, 143, 164, 606, 631
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 2 cut(s) 6, 253
Hpy8I GTNNAC 5 cut(s) 45, 143, 164, 606, 631
HpyAV CCTTC 5 cut(s) 150, 187, 470, 474, 673
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 3 cut(s) 296, 354, 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 3 cut(s) 149, 332, 402
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 3 cut(s) 94, 268, 358
Kzo9I GATC 1 cut(s) 337
Lsp1109I GCAGC 2 cut(s) 121, 308
LweI GCATC 4 cut(s) 49, 249, 283, 341
MaeII ACGT 1 cut(s) 249
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 6 cut(s) 93, 96, 268, 579, 585, 588
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 2 cut(s) 363, 469
MlyI GAGTC 1 cut(s) 274
Mph1103I ATGCAT 1 cut(s) 356
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 2 cut(s) 432, 673
MslI CAYNNNNRTG 2 cut(s) 169, 269
MspI CCGG 3 cut(s) 275, 341, 504
MspR9I CCNGG 2 cut(s) 105, 505
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 296
NciI CCSGG 1 cut(s) 505
NdeI CATATG 1 cut(s) 483
NdeII GATC 1 cut(s) 337
NlaIII CATG 3 cut(s) 94, 268, 358
NlaIV GGNNCC 4 cut(s) 99, 109, 241, 273
NsiI ATGCAT 1 cut(s) 356
NspI RCATGY 1 cut(s) 94
PciI ACATGT 1 cut(s) 90
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 4 cut(s) 136, 298, 531, 552
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PscI ACATGT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 4 cut(s) 99, 109, 241, 273
PspOMI GGGCCC 1 cut(s) 97
PspPI GGNCC 3 cut(s) 97, 98, 223
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 2 cut(s) 169, 269
SaqAI TTAA 2 cut(s) 432, 673
SatI GCNGC 4 cut(s) 135, 297, 530, 551
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 3 cut(s) 97, 98, 223
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 2 cut(s) 105, 505
SduI GDGCHC 1 cut(s) 101
SfaNI GCATC 4 cut(s) 49, 249, 283, 341
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 169, 269
Sse9I AATT 2 cut(s) 363, 469
SsiI CCGC 4 cut(s) 530, 548, 551, 563
StyD4I CCNGG 2 cut(s) 103, 503
TaiI ACGT 1 cut(s) 252
TaqI TCGA 1 cut(s) 254
TaqII GACCGA 1 cut(s) 211
TasI AATT 2 cut(s) 363, 469
TatI WGTACW 1 cut(s) 603
TauI GCSGC 2 cut(s) 532, 553
TfiI GAWTC 1 cut(s) 610
Tru1I TTAA 2 cut(s) 432, 673
Tru9I TTAA 2 cut(s) 432, 673
TscAI CASTG 1 cut(s) 628
TseI GCWGC 2 cut(s) 134, 296
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 1 cut(s) 505
TspRI CASTG 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 223
XceI RCATGY 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmnI GAANNNNTTC 1 cut(s) 160
Zsp2I ATGCAT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.