MD07G1231000.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003044
Physical Location & Seq
Reverse (-)
30520094 .. 30521790
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1231000.v1.1.491

Sequence Viewer

Length: 675 bp
ATGTCCAGAGAAAGGGAGGAAGTTGATGAGTTAGGCAAGAAGTTTAAGAGAGAAACTGATGCTTCTTCCCATTATCAAATGGGAAGAAGACACATGTTGGGCCCTCCTGGAACCCTAAACACCATCACTCCTTGTGCTGCCTGTAAACTCCTCAGACGAAGGTGTGCACAGGAATGCCCCTTCTCTCCTTACTTCTCTCCCCACGAACCCCAGAAGTTTGCTTCGGTTCATAAGGTCTTTGGTGCCAGCAACGTCTCGAAGATGCTCATGGAGGTGCCGGAGACTCAAAGAGCAGATGCAGCAAATAGTCTTGTTTATGAAGCAAATGTGAGGCTACGAGATCCGGTGTATGGATGCATGGGTGCAATTTCGGCTTTGCAACAACAAGTTCAATCTTTACAAGCTGAGCTCAACGCAGTAAGGGATGAGATACTTAAATACAAATACAGGGAAGCCAATCTCCTTCCATCTAATTCTCATCATATGGCTTTGTTCTCTACTTCCGGGGCTGTTTCTGCTGCTTCCTTGCCCCAGACCCCACAGCCACCCCCACCGCCGCCACTGCCACCTCCTATGCCTCCTACTTCATCTTCTTCTTCTATGTACACTAATCAACAAACCAGTGCTGCGGATTATAGCTCCCTTTCAAGTGAAAATGTTTCCTTCTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

225

Amino Acids

24.65

Weight (kDa)

8.26

Isoelectric Point (pI)

69.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 44 - 141 7.1e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 242, 274
AciI CCGC 3 cut(s) 554, 557, 629
AclWI GGATC 1 cut(s) 335
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 2 cut(s) 12, 350
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 2 cut(s) 392, 648
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 3 cut(s) 404, 409, 639
AluI AGCT 3 cut(s) 404, 409, 639
Alw21I GWGCWC 2 cut(s) 169, 411
Alw26I GTCTC 2 cut(s) 259, 275
Alw44I GTGCAC 1 cut(s) 165
AlwI GGATC 1 cut(s) 335
AoxI GGCC 1 cut(s) 100
ApaI GGGCCC 1 cut(s) 104
ApaLI GTGCAC 1 cut(s) 165
ApeKI GCWGC 4 cut(s) 137, 299, 518, 626
AspS9I GGNCC 2 cut(s) 100, 101
AsuC2I CCSGG 1 cut(s) 505
BaeGI GKGCMC 2 cut(s) 104, 169
BanI GGYRCC 2 cut(s) 242, 274
BanII GRGCYC 2 cut(s) 104, 411
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 2 cut(s) 169, 411
BbvI GCAGC 4 cut(s) 124, 311, 505, 613
BccI CCATC 2 cut(s) 131, 475
BciT130I CCWGG 1 cut(s) 108
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 259, 275
BisI GCNGC 5 cut(s) 138, 300, 519, 557, 627
BlpI GCTNAGC 1 cut(s) 405
BlsI GCNGC 5 cut(s) 139, 301, 520, 558, 628
Bme1390I CCNGG 2 cut(s) 108, 505
BmgT120I GGNCC 2 cut(s) 100, 101
BmiI GGNNCC 4 cut(s) 102, 112, 244, 276
BmrFI CCNGG 2 cut(s) 108, 505
BmsI GCATC 4 cut(s) 49, 252, 286, 344
BpiI GAAGAC 1 cut(s) 94
Bpu1102I GCTNAGC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 504
BsaWI WCCGGW 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 112, 142
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 350
Bse1I ACTGG 1 cut(s) 621
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 2 cut(s) 359, 430
BseLI CCNNNNNNNGG 2 cut(s) 12, 350
BseMII CTCAG 2 cut(s) 166, 396
BseNI ACTGG 1 cut(s) 621
BseRI GAGGAG 1 cut(s) 140
BseSI GKGCMC 2 cut(s) 104, 169
BseXI GCAGC 4 cut(s) 124, 311, 505, 613
BshFI GGCC 1 cut(s) 102
BshNI GGYRCC 2 cut(s) 242, 274
BsiHKAI GWGCWC 2 cut(s) 169, 411
BsiSI CCGG 3 cut(s) 278, 344, 504
BslI CCNNNNNNNGG 2 cut(s) 12, 350
BsmAI GTCTC 2 cut(s) 259, 275
BsmBI CGTCTC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 1 cut(s) 102
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 104, 169, 411
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 1 cut(s) 340
Bsp1720I GCTNAGC 1 cut(s) 405
BspACI CCGC 3 cut(s) 554, 557, 629
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 2 cut(s) 165, 397
BspLI GGNNCC 4 cut(s) 102, 112, 244, 276
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 2 cut(s) 242, 274
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 1 cut(s) 621
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 108
BstAUI TGTACA 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 2 cut(s) 152, 405
BstF5I GGATG 2 cut(s) 359, 430
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 2 cut(s) 259, 275
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 299, 371, 515, 562
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 2 cut(s) 106, 503
BstSLI GKGCMC 2 cut(s) 104, 169
BstV1I GCAGC 4 cut(s) 124, 311, 505, 613
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuRI GGCC 1 cut(s) 102
BtsCI GGATG 2 cut(s) 359, 430
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 2 cut(s) 560, 628
Cac8I GCNNGC 1 cut(s) 247
Cfr13I GGNCC 2 cut(s) 100, 101
Csp6I GTAC 1 cut(s) 604
CviAII CATG 3 cut(s) 94, 268, 358
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 2 cut(s) 152, 405
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Ecl136II GAGCTC 1 cut(s) 409
Eco24I GRGCYC 2 cut(s) 104, 411
Eco53kI GAGCTC 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 409
EcoO109I RGGNCCY 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 359
EcoT38I GRGCYC 2 cut(s) 104, 411
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 3 cut(s) 97, 271, 361
FatI CATG 3 cut(s) 93, 267, 357
FauNDI CATATG 1 cut(s) 483
Fnu4HI GCNGC 5 cut(s) 138, 300, 519, 557, 627
FokI GGATG 2 cut(s) 366, 437
FriOI GRGCYC 2 cut(s) 104, 411
Fsp4HI GCNGC 5 cut(s) 138, 300, 519, 557, 627
GluI GCNGC 5 cut(s) 138, 300, 519, 557, 627
HaeIII GGCC 1 cut(s) 102
HapII CCGG 3 cut(s) 278, 344, 504
Hin1II CATG 3 cut(s) 97, 271, 361
HinfI GANTC 1 cut(s) 283
HpaII CCGG 3 cut(s) 278, 344, 504
Hpy166II GTNNAC 3 cut(s) 146, 167, 606
Hpy188I TCNGA 1 cut(s) 155
Hpy188III TCNNGA 2 cut(s) 6, 256
Hpy8I GTNNAC 3 cut(s) 146, 167, 606
HpyAV CCTTC 4 cut(s) 153, 190, 473, 673
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 5 cut(s) 167, 299, 357, 365, 379
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 371, 515, 562
HpyF3I CTNAG 2 cut(s) 152, 405
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 3 cut(s) 97, 271, 361
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 644
Lsp1109I GCAGC 4 cut(s) 124, 311, 505, 613
LweI GCATC 4 cut(s) 49, 252, 286, 344
MaeII ACGT 1 cut(s) 252
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 7 cut(s) 57, 96, 99, 271, 582, 585, 588
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 3 cut(s) 104, 169, 411
MluCI AATT 2 cut(s) 366, 472
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 7 cut(s) 10, 114, 161, 265, 324, 579, 588
Mph1103I ATGCAT 1 cut(s) 359
MseI TTAA 3 cut(s) 45, 435, 673
MslI CAYNNNNRTG 2 cut(s) 172, 272
MspI CCGG 3 cut(s) 278, 344, 504
MspR9I CCNGG 2 cut(s) 108, 505
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 4 cut(s) 299, 371, 515, 562
NciI CCSGG 1 cut(s) 505
NdeI CATATG 1 cut(s) 483
NdeII GATC 1 cut(s) 340
NlaIII CATG 3 cut(s) 97, 271, 361
NlaIV GGNNCC 4 cut(s) 102, 112, 244, 276
NsiI ATGCAT 1 cut(s) 359
NspI RCATGY 1 cut(s) 97
PciI ACATGT 1 cut(s) 93
PctI GAATGC 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 5 cut(s) 139, 301, 520, 558, 628
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PscI ACATGT 1 cut(s) 93
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 4 cut(s) 102, 112, 244, 276
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 2 cut(s) 100, 101
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 2 cut(s) 172, 272
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 45, 435, 673
SatI GCNGC 5 cut(s) 138, 300, 519, 557, 627
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 2 cut(s) 100, 101
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 2 cut(s) 108, 505
SduI GDGCHC 3 cut(s) 104, 169, 411
SetI ASST 8 cut(s) 164, 237, 255, 276, 406, 411, 571, 641
SfaNI GCATC 4 cut(s) 49, 252, 286, 344
SmiMI CAYNNNNRTG 2 cut(s) 172, 272
Sse9I AATT 2 cut(s) 366, 472
SsiI CCGC 3 cut(s) 554, 557, 629
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 2 cut(s) 106, 503
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 257
TasI AATT 2 cut(s) 366, 472
TatI WGTACW 1 cut(s) 603
TauI GCSGC 1 cut(s) 559
Tru1I TTAA 3 cut(s) 45, 435, 673
Tru9I TTAA 3 cut(s) 45, 435, 673
TscAI CASTG 2 cut(s) 567, 628
TseI GCWGC 4 cut(s) 137, 299, 518, 626
TspDTI ATGAA 3 cut(s) 218, 333, 576
TspRI CASTG 2 cut(s) 567, 628
VneI GTGCAC 1 cut(s) 165
XceI RCATGY 1 cut(s) 97
Zsp2I ATGCAT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.