FvH4_3g45191

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002477
Physical Location & Seq
Reverse (-)
37557494 .. 37561521
4028 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45191.t2

Sequence Viewer

Length: 1302 bp
ATGGACTCAAACGCTGTACAGTCTTCGAGGGGTTATAGAGTTCAAGCTCCACTGGTTGACTCTGTATCATGCTATTGCAAAGTAGATGCAGGCTTGAAAACAGTTGCAGGGGCAAGAAAGTTTGTCCCAGGATCAAAACTTTGCATCCAGCCTGATATCAATCCAAACGCACACAGGGGGAAACAAAGAAGGGAAAGGACTAGAATCCAGCCACCCCTTCTGCCTGGCCTACCTGATGATCTTGCAATTGCTTGTTTAATCAGGGTCCCTCGGGTGGATCATAGAAAACTCCGTCTAGTTTGCAAAAGATGGTACCGCCTTCTGGCTGGAAACTTCTTTTATTCTCTTAGGAAAAGTCTTGGAATGGCAGAAGAGTGGGTTTATGTCATCAAAAGAGACCGTGATGGAAGAATCTCTTGGCATGCTTTTGATCCCACGTACCAGCTCTGGCAGCCACTTCCGCCCGTTCCTAATGAATATTCTGCAGCGCTTGGTTTTGGCTGTGCTGTCCTTAGTGGTTGCCACCTATATTTATTTGGAGGGAAAGATCCATTGAAGGGCTCTATGAGACGGGTTATTTTTTACAGTGCTCGGACAAATAAATGGCACAGGGCCCCAGATATGCTTCGGAAGCGGCACTTCTTTGGTGCTTGTGTTATTAATAACTGCCTTTATGTAGCTGGTGGAGAGTGTGAAGGAATTCAAAGGACTCTCCGTTCTGCTGAAATTTATGACCCTAACAAGAACCGGTGGAGCTTTATTTCAGATATGAGCACAGCTATGGTTCCCTTCATTGGGGTTGTTCACGATGGAATGTGGTTCCTAAAAGGACTTGGATCGCACCGTGAGGTAATGAGTGAAGCATATTCTCCTGAGGCTAACACTTGGAACCCCATTAGTGATGGGATGGTTGCTGGTTGGCGCAACCCAAGTATTTCCTTAAATGGGCAACTCTATGCTCTGGATTGCCGTGATGGGTGCAAGGTTAGGGTTTATGATCGGGTGACAGATTCATGGAACAAATGTATTGATAGCAAGCTTCATCTGGGTAGTTCTCGTGCTTTGGAGGCTGCTGCACTTGTTCCCCTCAATGGGAAGCTTTGCATTATTCGTAATAACATGAGCATCAGTTTAGTTGATGTTTCGAGTCCGGATAAACAGGTTGAAAGCAACCCTAACCTTTGGGAAAATATTGCTGGTAAAGGTCATTTCAGAACTCTAGTCTCAAATTTATGGTCAAGTATAGCAGGCCGAACTGGTTTGAAGAGTCACATTGTACACTGTCAAGTGCTTCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

434

Amino Acids

48.63

Weight (kDa)

9.54

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 18 - 176 1.1e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 27 - 141 3.1e-11 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 34 - 222 1.8e-18 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 65 - 179 9.4e-12 Calicin, beta-propeller domain
Kelch_1 PF01344 91 - 137 1e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccIII TCCGGA 1 cut(s) 1150
AciI CCGC 3 cut(s) 316, 461, 634
AclWI GGATC 5 cut(s) 139, 285, 425, 542, 844
AcsI RAATTY 3 cut(s) 699, 726, 1228
AfaI GTAC 4 cut(s) 18, 314, 440, 1278
AfeI AGCGCT 1 cut(s) 489
AfiI CCNNNNNNNGG 8 cut(s) 274, 322, 557, 794, 795, 945, 1091, 1092
AgeI ACCGGT 1 cut(s) 747
AgsI TTSAA 7 cut(s) 44, 97, 556, 704, 1166, 1264, 1295
AjnI CCWGG 2 cut(s) 127, 223
AjuI GAANNNNNNNTTGG 2 cut(s) 400, 432
AloI GAACNNNNNNTCC 2 cut(s) 700, 732
AluBI AGCT 7 cut(s) 47, 445, 680, 756, 779, 1039, 1099
AluI AGCT 7 cut(s) 47, 445, 680, 756, 779, 1039, 1099
Alw21I GWGCWC 2 cut(s) 592, 776
Alw26I GTCTC 3 cut(s) 390, 562, 1228
AlwI GGATC 5 cut(s) 139, 285, 425, 542, 844
Ama87I CYCGRG 1 cut(s) 270
Aor13HI TCCGGA 1 cut(s) 1150
Aor51HI AGCGCT 1 cut(s) 489
AoxI GGCC 3 cut(s) 226, 612, 1249
ApaI GGGCCC 1 cut(s) 616
ApeKI GCWGC 4 cut(s) 451, 485, 1070, 1073
ApoI RAATTY 3 cut(s) 699, 726, 1228
ArsI GACNNNNNNTTYG 2 cut(s) 1220, 1252
AseI ATTAAT 1 cut(s) 660
AsiGI ACCGGT 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 699
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 490, 924
AspS9I GGNCC 3 cut(s) 265, 612, 613
AsuHPI GGTGA 1 cut(s) 1015
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 2 cut(s) 563, 616
BauI CACGAG 1 cut(s) 1056
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 592, 776
BbvI GCAGC 4 cut(s) 463, 497, 1057, 1060
BccI CCATC 6 cut(s) 303, 398, 803, 896, 901, 968
BceAI ACGGC 1 cut(s) 954
BciT130I CCWGG 2 cut(s) 129, 225
BcoDI GTCTC 3 cut(s) 390, 562, 1228
BfaI CTAG 3 cut(s) 201, 296, 1220
BfmI CTRYAG 1 cut(s) 483
BfoI RGCGCY 1 cut(s) 491
BisI GCNGC 5 cut(s) 452, 486, 635, 1071, 1074
BlsI GCNGC 5 cut(s) 453, 487, 636, 1072, 1075
Bme1390I CCNGG 2 cut(s) 129, 225
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 265, 612, 613
BmiI GGNNCC 8 cut(s) 266, 267, 314, 614, 615, 786, 821, 890
BmrFI CCNGG 2 cut(s) 129, 225
BmsI GCATC 3 cut(s) 76, 153, 1134
BpiI GAAGAC 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 438
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 127, 269
BsaWI WCCGGW 2 cut(s) 747, 1150
Bsc4I CCNNNNNNNGG 8 cut(s) 274, 322, 557, 794, 795, 945, 1091, 1092
Bse118I RCCGGY 1 cut(s) 747
Bse1I ACTGG 2 cut(s) 57, 1261
Bse21I CCTNAGG 1 cut(s) 873
Bse8I GATNNNNATC 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 1150
BseBI CCWGG 2 cut(s) 129, 225
BseDI CCNNGG 2 cut(s) 127, 269
BseGI GGATG 2 cut(s) 144, 912
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 8 cut(s) 274, 322, 557, 794, 795, 945, 1091, 1092
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 2 cut(s) 57, 1261
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 4 cut(s) 463, 497, 1057, 1060
BsgI GTGCAG 1 cut(s) 1059
BshFI GGCC 3 cut(s) 228, 614, 1251
BshNI GGYRCC 1 cut(s) 312
BshTI ACCGGT 1 cut(s) 747
BsiHKAI GWGCWC 2 cut(s) 592, 776
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 2 cut(s) 748, 1151
BslFI GGGAC 2 cut(s) 110, 251
BslI CCNNNNNNNGG 8 cut(s) 274, 322, 557, 794, 795, 945, 1091, 1092
BsmAI GTCTC 3 cut(s) 390, 562, 1228
BsmBI CGTCTC 1 cut(s) 562
BsmFI GGGAC 2 cut(s) 110, 251
BsnI GGCC 3 cut(s) 228, 614, 1251
Bso31I GGTCTC 1 cut(s) 390
BsoBI CYCGRG 1 cut(s) 270
Bsp120I GGGCCC 1 cut(s) 612
Bsp1286I GDGCHC 4 cut(s) 563, 592, 616, 776
Bsp13I TCCGGA 1 cut(s) 1150
Bsp1407I TGTACA 2 cut(s) 16, 1276
Bsp143I GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
BspACI CCGC 3 cut(s) 316, 461, 634
BspANI GGCC 3 cut(s) 228, 614, 1251
BspCNI CTCAG 1 cut(s) 865
BspEI TCCGGA 1 cut(s) 1150
BspLI GGNNCC 8 cut(s) 266, 267, 314, 614, 615, 786, 821, 890
BspMAI CTGCAG 1 cut(s) 487
BspPI GGATC 5 cut(s) 139, 285, 425, 542, 844
BspT107I GGYRCC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 747
BsrGI TGTACA 2 cut(s) 16, 1276
BsrI ACTGG 2 cut(s) 57, 1261
BssAI RCCGGY 1 cut(s) 747
BssECI CCNNGG 2 cut(s) 127, 269
BssMI GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
BssSI CACGAG 1 cut(s) 1056
Bst2BI CACGAG 1 cut(s) 1056
Bst2UI CCWGG 2 cut(s) 129, 225
Bst4CI ACNGT 6 cut(s) 21, 103, 401, 587, 845, 1283
Bst6I CTCTTC 2 cut(s) 366, 1259
BstAUI TGTACA 2 cut(s) 16, 1276
BstBAI YACGTR 1 cut(s) 438
BstC8I GCNNGC 4 cut(s) 91, 423, 1037, 1249
BstDEI CTNAG 3 cut(s) 347, 512, 873
BstF5I GGATG 2 cut(s) 144, 912
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 2 cut(s) 490, 924
BstKTI GATC 7 cut(s) 134, 241, 280, 433, 550, 839, 1000
BstMAI GTCTC 3 cut(s) 390, 562, 1228
BstMBI GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
BstMWI GCNNNNNNNGC 4 cut(s) 451, 460, 631, 1067
BstNI CCWGG 2 cut(s) 129, 225
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 2 cut(s) 127, 223
BstSFI CTRYAG 1 cut(s) 483
BstSLI GKGCMC 1 cut(s) 616
BstV1I GCAGC 4 cut(s) 463, 497, 1057, 1060
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 3 cut(s) 228, 614, 1251
BtsCI GGATG 2 cut(s) 144, 912
BtsIMutI CAGTG 3 cut(s) 50, 592, 1279
Cac8I GCNNGC 4 cut(s) 91, 423, 1037, 1249
CfoI GCGC 2 cut(s) 490, 924
Cfr10I RCCGGY 1 cut(s) 747
Cfr13I GGNCC 3 cut(s) 265, 612, 613
Csp6I GTAC 4 cut(s) 17, 313, 439, 1277
CspAI ACCGGT 1 cut(s) 747
CviAII CATG 5 cut(s) 69, 422, 1014, 1120, 1299
CviQI GTAC 4 cut(s) 17, 313, 439, 1277
DdeI CTNAG 3 cut(s) 347, 512, 873
DpnI GATC 7 cut(s) 133, 240, 279, 432, 549, 838, 999
DpnII GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
Eam1104I CTCTTC 2 cut(s) 366, 1259
EarI CTCTTC 2 cut(s) 366, 1259
EciI GGCGGA 1 cut(s) 450
Eco24I GRGCYC 2 cut(s) 563, 616
Eco31I GGTCTC 1 cut(s) 390
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 265
Eco47III AGCGCT 1 cut(s) 489
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 270
EcoO109I RGGNCCY 3 cut(s) 265, 612, 613
EcoRI GAATTC 1 cut(s) 699
EcoRII CCWGG 2 cut(s) 127, 223
EcoRV GATATC 1 cut(s) 157
EcoT38I GRGCYC 2 cut(s) 563, 616
Esp3I CGTCTC 1 cut(s) 562
FaeI CATG 5 cut(s) 72, 425, 1017, 1123, 1302
FalI AAGNNNNNCTT 4 cut(s) 400, 432, 623, 655
FaqI GGGAC 2 cut(s) 110, 251
FatI CATG 5 cut(s) 68, 421, 1013, 1119, 1298
Fnu4HI GCNGC 5 cut(s) 452, 486, 635, 1071, 1074
FokI GGATG 2 cut(s) 131, 919
FriOI GRGCYC 2 cut(s) 563, 616
Fsp4HI GCNGC 5 cut(s) 452, 486, 635, 1071, 1074
FspBI CTAG 3 cut(s) 201, 296, 1220
GlaI GCGC 2 cut(s) 489, 923
GluI GCNGC 5 cut(s) 452, 486, 635, 1071, 1074
HaeII RGCGCY 1 cut(s) 491
HaeIII GGCC 3 cut(s) 228, 614, 1251
HapII CCGG 2 cut(s) 748, 1151
HhaI GCGC 2 cut(s) 490, 924
Hin1II CATG 5 cut(s) 72, 425, 1017, 1123, 1302
Hin6I GCGC 2 cut(s) 488, 922
HinP1I GCGC 2 cut(s) 488, 922
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 2 cut(s) 1037, 1097
HinfI GANTC 8 cut(s) 5, 59, 204, 411, 709, 1010, 1147, 1267
HpaII CCGG 2 cut(s) 748, 1151
HphI GGTGA 1 cut(s) 1015
Hpy166II GTNNAC 3 cut(s) 58, 805, 1279
Hpy188I TCNGA 4 cut(s) 594, 630, 766, 1214
Hpy188III TCNNGA 4 cut(s) 806, 872, 962, 1151
Hpy8I GTNNAC 3 cut(s) 58, 805, 1279
HpyAV CCTTC 6 cut(s) 183, 227, 329, 550, 689, 799
HpyCH4III ACNGT 6 cut(s) 21, 103, 401, 587, 845, 1283
HpyCH4IV ACGT 1 cut(s) 437
HpyF10VI GCNNNNNNNGC 4 cut(s) 451, 460, 631, 1067
HpyF3I CTNAG 3 cut(s) 347, 512, 873
HpySE526I ACGT 1 cut(s) 437
Hsp92II CATG 5 cut(s) 72, 425, 1017, 1123, 1302
HspAI GCGC 2 cut(s) 488, 922
KflI GGGWCCC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 1150
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
LmnI GCTCC 2 cut(s) 52, 753
Lsp1109I GCAGC 4 cut(s) 463, 497, 1057, 1060
LweI GCATC 3 cut(s) 76, 153, 1134
MaeI CTAG 3 cut(s) 201, 296, 1220
MaeII ACGT 1 cut(s) 437
MaeIII GTNAC 2 cut(s) 1003, 1268
MalI GATC 7 cut(s) 133, 240, 279, 432, 549, 838, 999
MboI GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
MboII GAAGA 4 cut(s) 15, 383, 420, 1276
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 4 cut(s) 563, 592, 616, 776
MluCI AATT 4 cut(s) 246, 699, 726, 1228
MlyI GAGTC 4 cut(s) 53, 703, 1156, 1276
MnlI CCTC 7 cut(s) 21, 279, 533, 841, 868, 1060, 1097
MroI TCCGGA 1 cut(s) 1150
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 3 cut(s) 257, 660, 941
MslI CAYNNNNRTG 1 cut(s) 779
MspI CCGG 2 cut(s) 748, 1151
MspR9I CCNGG 2 cut(s) 129, 225
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 2 cut(s) 129, 225
MwoI GCNNNNNNNGC 4 cut(s) 451, 460, 631, 1067
NdeII GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
NlaIII CATG 5 cut(s) 72, 425, 1017, 1123, 1302
NlaIV GGNNCC 8 cut(s) 266, 267, 314, 614, 615, 786, 821, 890
NmuCI GTSAC 2 cut(s) 1003, 1268
NspI RCATGY 1 cut(s) 425
PaeI GCATGC 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 3 cut(s) 204, 411, 1010
PinAI ACCGGT 1 cut(s) 747
PkrI GCNGC 5 cut(s) 453, 487, 636, 1072, 1075
PleI GAGTC 4 cut(s) 53, 703, 1155, 1275
PpsI GAGTC 4 cut(s) 53, 703, 1155, 1275
Ppu21I YACGTR 1 cut(s) 438
PpuMI RGGWCCY 1 cut(s) 265
PshBI ATTAAT 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 127, 223
PspGI CCWGG 2 cut(s) 127, 223
PspN4I GGNNCC 8 cut(s) 266, 267, 314, 614, 615, 786, 821, 890
PspOMI GGGCCC 1 cut(s) 612
PspPI GGNCC 3 cut(s) 265, 612, 613
PspPPI RGGWCCY 1 cut(s) 265
PstI CTGCAG 1 cut(s) 487
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 4 cut(s) 18, 314, 440, 1278
RsaNI GTAC 4 cut(s) 17, 313, 439, 1277
RseI CAYNNNNRTG 1 cut(s) 779
SaqAI TTAA 3 cut(s) 257, 660, 941
SatI GCNGC 5 cut(s) 452, 486, 635, 1071, 1074
Sau3AI GATC 7 cut(s) 131, 238, 277, 430, 547, 836, 997
Sau96I GGNCC 3 cut(s) 265, 612, 613
SchI GAGTC 4 cut(s) 53, 703, 1156, 1276
ScrFI CCNGG 2 cut(s) 129, 225
SduI GDGCHC 4 cut(s) 563, 592, 616, 776
SfaNI GCATC 3 cut(s) 76, 153, 1134
SfcI CTRYAG 1 cut(s) 483
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 779
SphI GCATGC 1 cut(s) 425
Sse9I AATT 4 cut(s) 246, 699, 726, 1228
SsiI CCGC 3 cut(s) 316, 461, 634
SspI AATATT 2 cut(s) 479, 1192
SspMI CTAG 3 cut(s) 201, 296, 1220
StyD4I CCNGG 2 cut(s) 127, 223
TaaI ACNGT 6 cut(s) 21, 103, 401, 587, 845, 1283
TaiI ACGT 1 cut(s) 440
TaqI TCGA 2 cut(s) 26, 1145
TasI AATT 4 cut(s) 246, 699, 726, 1228
TatI WGTACW 2 cut(s) 16, 1276
TauI GCSGC 1 cut(s) 637
TfiI GAWTC 3 cut(s) 204, 411, 1010
Tru1I TTAA 3 cut(s) 257, 660, 941
Tru9I TTAA 3 cut(s) 257, 660, 941
TscAI CASTG 3 cut(s) 57, 592, 1286
TseFI GTSAC 2 cut(s) 1003, 1268
TseI GCWGC 4 cut(s) 451, 485, 1070, 1073
Tsp45I GTSAC 2 cut(s) 1003, 1268
TspDTI ATGAA 4 cut(s) 489, 781, 1002, 1031
TspGWI ACGGA 2 cut(s) 281, 704
TspRI CASTG 3 cut(s) 57, 592, 1286
VpaK11BI GGWCC 1 cut(s) 265
VspI ATTAAT 1 cut(s) 660
XapI RAATTY 3 cut(s) 699, 726, 1228
XceI RCATGY 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 3 cut(s) 201, 296, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.