RchiOBHm_Chr5g0082801

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002477
Physical Location & Seq
Reverse (-)
88667829 .. 88671980
4152 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35699

Sequence Viewer

Length: 1305 bp
ATGGACCCAAACGCTGTGCAGTCTTCGAGGGGATACAGAGTTCAAGCTCCACTGGTTGACTCCGTATCATGTTATTGCAAAGTAGATGCAGGCTTAAAAACAGTTGCAGGGGCAAGAAAGTTTGTCCCAGGATCAAAACTTTGTATCCAGCCTGATATCAATCCAAACGCACACAGGGGAAAAAGTTCACGTAGGGAAAGGACCAGAATCCAGCCACCCCTTCTGCCTGGCCTACCTGATGATCTTGCAATTGCTTGTTTAATCAGAGTTCCTCGGGTGGAGCATAGAAAACTCCGTCTAGTTTGCAAAAGATGGTATCGCCTTCTGGCTGGAAACTTCTTTTATTCTCTTAGGAAAAGTCTTGGAATGGCAGAAGAGTGGGTTTATGTCATCAAAAGAGACCGTGATGGAAGAATCTCTTGGCATGCTTTTGATCCCACGTACCAGCTCTGGCAGCCACTTCCGCCCGTTCCTGGTGAATATTCTGCAGCACTTGGTTTTGGCTGTGCTGTCCTTAGTGGTTGCCACCTGTACTTATTTGGAGGAAAAGATCCATTGAAGGGATCTATGAGACGGGTTATTTTTTACAGTGCTCGGACAAATAAATGGCACAGGGCACCAGATATGCTTCGAAAGCGGCATTTCTTTGGTGCTTGTGTTATTAATAACTGCCTTTATGTAGCTGGTGGAGAGTGTGAAGGAATTCAAAGGACTCTCCGTTCTGCTGAAATTTATGACCCTAACAAGAACCGGTGGAGCTTTATTTCAGATATGAGCACAGCTATGGTTCCCTTTATTGGGGTTGTTCACGATGGAATGTGGTTCCTAAAAGGACTTGGATCGCACCGTGAAGTAATGAGTGAAGCCTATTCTCCTGAAGCTAACACTTGGAACCCCATTAGTGATGGGATGGTTGCTGGTTGGCGCAACCCAAGTATTTCTTTAAATGGGCAACTCTATGCTCTGGATTGCCGTGATGGGTGCAAGGTTAGGGTTTATGATAGAGTGACAGATTCATGGAACAAATTTATTGATAGCAAGCTTCATCTGGGCAGTTCTCGTGCTTTGGAGGCTGCTGCACTTGTTCCCCTCAATGGGAAGCTTTGCATTATTCGTAATAACATGAGCATCAGTTTAGTTGATGTTTCAAGTCCGGATAAACAGGTTGAAAGCAACCCTAGCCTTTGGGAGAATATTGCTGGTAAAGGCCATTTCAGAACTCTAGTCTCGAATTTATGGTCAAGTATAGCAGGCCGAACTGGTTTGAAGAGTCACATTGTACACTGTCAAGTGCTCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

434

Amino Acids

48.66

Weight (kDa)

9.57

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 76 - 112 4e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 140 - 298 4.5e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 151 - 263 2.3e-10 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 167 - 344 8.1e-18 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 187 - 301 2.2e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 213 - 259 1.6e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 616
AccIII TCCGGA 1 cut(s) 1153
AciI CCGC 2 cut(s) 464, 637
AclWI GGATC 5 cut(s) 139, 428, 545, 571, 847
AcsI RAATTY 4 cut(s) 702, 729, 1025, 1231
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 3 cut(s) 443, 533, 1281
AfiI CCNNNNNNNGG 6 cut(s) 473, 560, 797, 798, 1094, 1095
AgeI ACCGGT 1 cut(s) 750
AgsI TTSAA 6 cut(s) 44, 559, 707, 1149, 1169, 1267
AjnI CCWGG 3 cut(s) 127, 226, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AloI GAACNNNNNNTCC 4 cut(s) 24, 56, 703, 735
AluBI AGCT 8 cut(s) 47, 448, 683, 759, 782, 881, 1042, 1102
AluI AGCT 8 cut(s) 47, 448, 683, 759, 782, 881, 1042, 1102
Alw21I GWGCWC 3 cut(s) 595, 779, 1296
Alw26I GTCTC 3 cut(s) 393, 565, 1231
AlwI GGATC 5 cut(s) 139, 428, 545, 571, 847
Ama87I CYCGRG 1 cut(s) 273
Aor13HI TCCGGA 1 cut(s) 1153
AoxI GGCC 3 cut(s) 229, 1207, 1252
ApeKI GCWGC 4 cut(s) 454, 488, 1073, 1076
ApoI RAATTY 4 cut(s) 702, 729, 1025, 1231
ArsI GACNNNNNNTTYG 2 cut(s) 1223, 1255
AseI ATTAAT 1 cut(s) 663
AsiGI ACCGGT 1 cut(s) 750
Asp700I GAANNNNTTC 2 cut(s) 184, 702
AspLEI GCGC 1 cut(s) 927
AspS9I GGNCC 2 cut(s) 4, 201
AsuHPI GGTGA 1 cut(s) 488
AsuII TTCGAA 1 cut(s) 631
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 2 cut(s) 4, 201
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 616
BauI CACGAG 1 cut(s) 1059
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 3 cut(s) 595, 779, 1296
BbvI GCAGC 4 cut(s) 466, 500, 1060, 1063
BccI CCATC 6 cut(s) 306, 401, 806, 899, 904, 971
BceAI ACGGC 1 cut(s) 957
BciT130I CCWGG 3 cut(s) 129, 228, 474
BciVI GTATCC 2 cut(s) 26, 155
BcoDI GTCTC 3 cut(s) 393, 565, 1231
BfaI CTAG 3 cut(s) 299, 1179, 1223
BfmI CTRYAG 1 cut(s) 486
BfuI GTATCC 2 cut(s) 26, 155
BisI GCNGC 5 cut(s) 455, 489, 638, 1074, 1077
BlsI GCNGC 5 cut(s) 456, 490, 639, 1075, 1078
Bme1390I CCNGG 3 cut(s) 129, 228, 474
Bme18I GGWCC 2 cut(s) 4, 201
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 4, 201
BmiI GGNNCC 5 cut(s) 6, 618, 789, 824, 893
BmrFI CCNGG 3 cut(s) 129, 228, 474
BmsI GCATC 2 cut(s) 76, 1137
BpiI GAAGAC 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 631
BsaAI YACGTR 2 cut(s) 191, 441
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 127, 272
BsaWI WCCGGW 2 cut(s) 750, 1153
Bsc4I CCNNNNNNNGG 6 cut(s) 473, 560, 797, 798, 1094, 1095
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 2 cut(s) 57, 1264
Bse8I GATNNNNATC 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 1153
BseBI CCWGG 3 cut(s) 129, 228, 474
BseDI CCNNGG 2 cut(s) 127, 272
BseGI GGATG 1 cut(s) 915
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 6 cut(s) 473, 560, 797, 798, 1094, 1095
BseNI ACTGG 2 cut(s) 57, 1264
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 4 cut(s) 466, 500, 1060, 1063
BsgI GTGCAG 2 cut(s) 38, 1062
BshFI GGCC 3 cut(s) 231, 1209, 1254
BshNI GGYRCC 1 cut(s) 616
BshTI ACCGGT 1 cut(s) 750
BsiHKAI GWGCWC 3 cut(s) 595, 779, 1296
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 2 cut(s) 751, 1154
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 6 cut(s) 473, 560, 797, 798, 1094, 1095
BsmAI GTCTC 3 cut(s) 393, 565, 1231
BsmBI CGTCTC 1 cut(s) 565
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 3 cut(s) 231, 1209, 1254
Bso31I GGTCTC 1 cut(s) 393
BsoBI CYCGRG 1 cut(s) 273
Bsp119I TTCGAA 1 cut(s) 631
Bsp1286I GDGCHC 4 cut(s) 595, 619, 779, 1296
Bsp13I TCCGGA 1 cut(s) 1153
Bsp1407I TGTACA 1 cut(s) 1279
Bsp143I GATC 6 cut(s) 131, 241, 433, 550, 563, 839
BspACI CCGC 2 cut(s) 464, 637
BspANI GGCC 3 cut(s) 231, 1209, 1254
BspEI TCCGGA 1 cut(s) 1153
BspLI GGNNCC 5 cut(s) 6, 618, 789, 824, 893
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 5 cut(s) 139, 428, 545, 571, 847
BspT104I TTCGAA 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 750
BsrGI TGTACA 1 cut(s) 1279
BsrI ACTGG 2 cut(s) 57, 1264
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 2 cut(s) 127, 272
BssMI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
BssSI CACGAG 1 cut(s) 1059
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 3 cut(s) 129, 228, 474
Bst4CI ACNGT 5 cut(s) 103, 404, 590, 848, 1286
Bst6I CTCTTC 2 cut(s) 369, 1262
BstAUI TGTACA 1 cut(s) 1279
BstBAI YACGTR 2 cut(s) 191, 441
BstBI TTCGAA 1 cut(s) 631
BstC8I GCNNGC 4 cut(s) 91, 426, 1040, 1252
BstDEI CTNAG 2 cut(s) 350, 515
BstF5I GGATG 1 cut(s) 915
BstHHI GCGC 1 cut(s) 927
BstKTI GATC 6 cut(s) 134, 244, 436, 553, 566, 842
BstMAI GTCTC 3 cut(s) 393, 565, 1231
BstMBI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
BstMWI GCNNNNNNNGC 5 cut(s) 454, 463, 634, 1070, 1179
BstNI CCWGG 3 cut(s) 129, 228, 474
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 127, 226, 472
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 4 cut(s) 466, 500, 1060, 1063
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 550, 563
BstYI RGATCY 2 cut(s) 550, 563
BsuI GTATCC 2 cut(s) 26, 155
BsuRI GGCC 3 cut(s) 231, 1209, 1254
BtsCI GGATG 1 cut(s) 915
BtsIMutI CAGTG 3 cut(s) 50, 595, 1282
Cac8I GCNNGC 4 cut(s) 91, 426, 1040, 1252
CfoI GCGC 1 cut(s) 927
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 2 cut(s) 4, 201
Csp6I GTAC 3 cut(s) 442, 532, 1280
CspAI ACCGGT 1 cut(s) 750
CviAII CATG 5 cut(s) 69, 425, 1017, 1123, 1302
CviQI GTAC 3 cut(s) 442, 532, 1280
DdeI CTNAG 2 cut(s) 350, 515
DpnI GATC 6 cut(s) 133, 243, 435, 552, 565, 841
DpnII GATC 6 cut(s) 131, 241, 433, 550, 563, 839
DraI TTTAAA 1 cut(s) 945
Eam1104I CTCTTC 2 cut(s) 369, 1262
EarI CTCTTC 2 cut(s) 369, 1262
EciI GGCGGA 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 4, 201
Eco57I CTGAAG 1 cut(s) 897
Eco88I CYCGRG 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 702
EcoRII CCWGG 3 cut(s) 127, 226, 472
EcoRV GATATC 1 cut(s) 157
Esp3I CGTCTC 1 cut(s) 565
FaeI CATG 5 cut(s) 72, 428, 1020, 1126, 1305
FalI AAGNNNNNCTT 4 cut(s) 403, 435, 925, 957
FaqI GGGAC 1 cut(s) 110
FatI CATG 5 cut(s) 68, 424, 1016, 1122, 1301
Fnu4HI GCNGC 5 cut(s) 455, 489, 638, 1074, 1077
FokI GGATG 1 cut(s) 922
Fsp4HI GCNGC 5 cut(s) 455, 489, 638, 1074, 1077
FspBI CTAG 3 cut(s) 299, 1179, 1223
GlaI GCGC 1 cut(s) 926
GluI GCNGC 5 cut(s) 455, 489, 638, 1074, 1077
HaeIII GGCC 3 cut(s) 231, 1209, 1254
HapII CCGG 2 cut(s) 751, 1154
HhaI GCGC 1 cut(s) 927
Hin1II CATG 5 cut(s) 72, 428, 1020, 1126, 1305
Hin6I GCGC 1 cut(s) 925
HinP1I GCGC 1 cut(s) 925
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 2 cut(s) 1040, 1100
HinfI GANTC 6 cut(s) 59, 207, 414, 712, 1013, 1270
HpaII CCGG 2 cut(s) 751, 1154
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 4 cut(s) 58, 188, 808, 1282
Hpy188I TCNGA 4 cut(s) 266, 597, 769, 1217
Hpy188III TCNNGA 5 cut(s) 809, 875, 965, 1154, 1228
Hpy8I GTNNAC 4 cut(s) 58, 188, 808, 1282
HpyAV CCTTC 4 cut(s) 230, 332, 553, 692
HpyCH4III ACNGT 5 cut(s) 103, 404, 590, 848, 1286
HpyCH4IV ACGT 2 cut(s) 190, 440
HpyF10VI GCNNNNNNNGC 5 cut(s) 454, 463, 634, 1070, 1179
HpyF3I CTNAG 2 cut(s) 350, 515
HpySE526I ACGT 2 cut(s) 190, 440
Hsp92II CATG 5 cut(s) 72, 428, 1020, 1126, 1305
HspAI GCGC 1 cut(s) 925
Kpn2I TCCGGA 1 cut(s) 1153
Kzo9I GATC 6 cut(s) 131, 241, 433, 550, 563, 839
LmnI GCTCC 4 cut(s) 52, 280, 756, 1299
Lsp1109I GCAGC 4 cut(s) 466, 500, 1060, 1063
LweI GCATC 2 cut(s) 76, 1137
MaeI CTAG 3 cut(s) 299, 1179, 1223
MaeII ACGT 2 cut(s) 190, 440
MaeIII GTNAC 2 cut(s) 1006, 1271
MalI GATC 6 cut(s) 133, 243, 435, 552, 565, 841
MboI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
MboII GAAGA 4 cut(s) 15, 386, 423, 1279
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 2 cut(s) 550, 563
MhlI GDGCHC 4 cut(s) 595, 619, 779, 1296
MluCI AATT 5 cut(s) 249, 702, 729, 1025, 1231
MlyI GAGTC 3 cut(s) 53, 706, 1279
MnlI CCTC 5 cut(s) 21, 282, 536, 1063, 1100
MroI TCCGGA 1 cut(s) 1153
MroXI GAANNNNTTC 2 cut(s) 184, 702
MseI TTAA 4 cut(s) 95, 260, 663, 944
MslI CAYNNNNRTG 1 cut(s) 782
MspI CCGG 2 cut(s) 751, 1154
MspR9I CCNGG 3 cut(s) 129, 228, 474
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 3 cut(s) 129, 228, 474
MwoI GCNNNNNNNGC 5 cut(s) 454, 463, 634, 1070, 1179
NdeII GATC 6 cut(s) 131, 241, 433, 550, 563, 839
NlaIII CATG 5 cut(s) 72, 428, 1020, 1126, 1305
NlaIV GGNNCC 5 cut(s) 6, 618, 789, 824, 893
NmuCI GTSAC 2 cut(s) 1006, 1271
NspI RCATGY 1 cut(s) 428
NspV TTCGAA 1 cut(s) 631
PaeI GCATGC 1 cut(s) 428
PdmI GAANNNNTTC 2 cut(s) 184, 702
PfeI GAWTC 3 cut(s) 207, 414, 1013
PinAI ACCGGT 1 cut(s) 750
PkrI GCNGC 5 cut(s) 456, 490, 639, 1075, 1078
PleI GAGTC 3 cut(s) 53, 706, 1278
PpsI GAGTC 3 cut(s) 53, 706, 1278
Ppu21I YACGTR 2 cut(s) 191, 441
PshBI ATTAAT 1 cut(s) 663
Psp6I CCWGG 3 cut(s) 127, 226, 472
PspGI CCWGG 3 cut(s) 127, 226, 472
PspN4I GGNNCC 5 cut(s) 6, 618, 789, 824, 893
PspPI GGNCC 2 cut(s) 4, 201
PstI CTGCAG 1 cut(s) 490
PsuI RGATCY 2 cut(s) 550, 563
RsaI GTAC 3 cut(s) 443, 533, 1281
RsaNI GTAC 3 cut(s) 442, 532, 1280
RseI CAYNNNNRTG 1 cut(s) 782
SaqAI TTAA 4 cut(s) 95, 260, 663, 944
SatI GCNGC 5 cut(s) 455, 489, 638, 1074, 1077
Sau3AI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
Sau96I GGNCC 2 cut(s) 4, 201
SchI GAGTC 3 cut(s) 53, 706, 1279
ScrFI CCNGG 3 cut(s) 129, 228, 474
SduI GDGCHC 4 cut(s) 595, 619, 779, 1296
SfaNI GCATC 2 cut(s) 76, 1137
SfcI CTRYAG 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 631
SinI GGWCC 2 cut(s) 4, 201
SmiMI CAYNNNNRTG 1 cut(s) 782
SphI GCATGC 1 cut(s) 428
Sse9I AATT 5 cut(s) 249, 702, 729, 1025, 1231
SsiI CCGC 2 cut(s) 464, 637
SspI AATATT 2 cut(s) 482, 1195
SspMI CTAG 3 cut(s) 299, 1179, 1223
StyD4I CCNGG 3 cut(s) 127, 226, 472
TaaI ACNGT 5 cut(s) 103, 404, 590, 848, 1286
TaiI ACGT 2 cut(s) 193, 443
TaqI TCGA 3 cut(s) 26, 631, 1229
TasI AATT 5 cut(s) 249, 702, 729, 1025, 1231
TatI WGTACW 2 cut(s) 531, 1279
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 3 cut(s) 207, 414, 1013
Tru1I TTAA 4 cut(s) 95, 260, 663, 944
Tru9I TTAA 4 cut(s) 95, 260, 663, 944
TscAI CASTG 3 cut(s) 57, 595, 1289
TseFI GTSAC 2 cut(s) 1006, 1271
TseI GCWGC 4 cut(s) 454, 488, 1073, 1076
Tsp45I GTSAC 2 cut(s) 1006, 1271
TspDTI ATGAA 2 cut(s) 1005, 1034
TspGWI ACGGA 3 cut(s) 52, 284, 707
TspRI CASTG 3 cut(s) 57, 595, 1289
VpaK11BI GGWCC 2 cut(s) 4, 201
VspI ATTAAT 1 cut(s) 663
XapI RAATTY 4 cut(s) 702, 729, 1025, 1231
XceI RCATGY 1 cut(s) 428
XmnI GAANNNNTTC 2 cut(s) 184, 702
XspI CTAG 3 cut(s) 299, 1179, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.