MD15G1175400.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002477
Physical Location & Seq
Reverse (-)
13625001 .. 13627995
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1175400.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGACAGAAGGATACAGCCTCCGTTGGTTGATTCAACAGCTTGCTTATGTAGAGTAGATGCTGGCCTTAAAACTGTTGCTGGAGCTAAAAAGTATGTCCCTGGGGCAAAGCTCTGTCTTCAACCCGACATTAAATCATCCATTCATCCAACTAGGAGTAAGCCTTCGCGTGGGGAGAGTAGCCGCATTCAACCCCCTCTGCTTCCTGGACTCCCAGATGATCTTGCTTTTGCTTGCCTAGTTCGGGTCCCAAGAGTTGAGCATCGTAAGCTCCGCCTGGTTTGCAAAAGATGGTACCGTCTTTTGGCTGCTAACTTCTTTTACTCCCTCCGTAGGAACCTTGGCATTGCAGAAGAATGGATATATTTAATTAAGAGGGATGGAGAAGGGAAAGTCTCTTGGCATGCTTTTGATCCCGTATACCAGCTCTGGCAACCCCTCCCTCCTGTCCCTAAAGAATATTCTGCAGCCCTGGGGTTTGGTTGTGCTGTACTCGGTGGCTGTCACCTGTATTTATTTGGAGGAAAAGACCCGCTAAAGGGATCAATGAGGCGAGTCATTTTTTATAATGCCAGGACCAATAAATGGCACCGTGCCCCAGATATGCTTCGGCGGCGCCATTTCTTTGGCTCGTGCGTTATAAATAACTGCTTGTATGTTGCCGGCGGGGAGAATGGGGGAATACATCGGTCCCTGAAATCAGCTGAAGTCTATGATCCCAACAAGAATAGATGGTCCTTCATTTCAGAGATGAGCACCGGAATGGCTCCCTACATTGGGGTTGTTTACGAAGGCAAATGGTTCTTGAAGGGGTTAGGGTCTCATCGACAAGTTTTGAGCGAGGTCTACCAACCAGAAAACGACAGCTGGTACTCTGTGTATGACGGAATGGTTGCAGGCTGGAGGAATCCAAGTGTTGCTTTAAACGGGCATCTCTATGCTTCGGAATGCAAAGATGGCTGCAAACTTAGAGTTTATGACAAAGCGACGGATTCTTGGAGCAAGCATATTGACAGCAAGATGCATTTAGGTAATTCTAAGGCTTTGGAGGCAGCTTCCCTTGTTCCCCTCAATGGCAAACTTTGCATCATTCGGAATAACATGAGCATCTCGCTGGTTGATGTTTCGAAATCCAGCGATGTTGGCGAGGCAAGTGCTGAATATCTATGGGAAACTATAGCAGGTAAGGGGCAGTTCAGGACCTTGGTCACGAATCTGTGGTCAAACCTCGCCGGCAGGAACCGCCTTAAAAGTCACATAGTTCACTGCCAGGTTCTTCAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

429

Amino Acids

48.1

Weight (kDa)

9.54

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 69 - 103 8.6e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 128 - 292 5.7e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 149 - 338 2e-20 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 180 - 291 7.6e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 205 - 252 1.2e-15 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 567, 641
Acc36I ACCTGC 1 cut(s) 1172
Acc65I GGTACC 1 cut(s) 294
AccB1I GGYRCC 3 cut(s) 294, 588, 615
AccB7I CCANNNNNTGG 1 cut(s) 585
AccI GTMKAC 2 cut(s) 420, 846
AccII CGCG 1 cut(s) 169
AciI CCGC 6 cut(s) 184, 274, 533, 613, 666, 1243
AclWI GGATC 3 cut(s) 407, 550, 710
AcuI CTGAAG 1 cut(s) 726
AcyI GRCGYC 1 cut(s) 616
AfaI GTAC 3 cut(s) 296, 492, 872
AgsI TTSAA 5 cut(s) 36, 122, 191, 808, 1280
AjnI CCWGG 6 cut(s) 100, 205, 276, 471, 572, 1269
AluBI AGCT 8 cut(s) 41, 86, 112, 271, 427, 704, 867, 1055
AluI AGCT 8 cut(s) 41, 86, 112, 271, 427, 704, 867, 1055
Alw21I GWGCWC 1 cut(s) 758
Alw26I GTCTC 2 cut(s) 400, 825
AlwI GGATC 3 cut(s) 407, 550, 710
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 4 cut(s) 308, 467, 960, 1052
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
Asp718I GGTACC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 618
AspS9I GGNCC 5 cut(s) 247, 576, 690, 735, 1200
AsuHPI GGTGA 1 cut(s) 497
AsuII TTCGAA 1 cut(s) 1127
AvaII GGWCC 5 cut(s) 247, 576, 690, 735, 1200
BaeGI GKGCMC 1 cut(s) 598
BanI GGYRCC 3 cut(s) 294, 588, 615
BauI CACGAG 1 cut(s) 631
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 758
BbvI GCAGC 4 cut(s) 295, 479, 947, 1064
BccI CCATC 4 cut(s) 285, 374, 726, 950
BcgI CGANNNNNNTGC 2 cut(s) 1136, 1170
BciT130I CCWGG 6 cut(s) 102, 207, 278, 473, 574, 1271
BciVI GTATCC 1 cut(s) 6
BcoDI GTCTC 2 cut(s) 400, 825
BfaI CTAG 2 cut(s) 153, 239
BfmI CTRYAG 2 cut(s) 465, 1176
BfoI RGCGCY 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 1172
BfuI GTATCC 1 cut(s) 6
BisI GCNGC 6 cut(s) 184, 309, 468, 614, 961, 1053
BlsI GCNGC 6 cut(s) 185, 310, 469, 615, 962, 1054
Bme1390I CCNGG 6 cut(s) 102, 207, 278, 473, 574, 1271
Bme18I GGWCC 5 cut(s) 247, 576, 690, 735, 1200
BmgT120I GGNCC 5 cut(s) 247, 576, 690, 735, 1200
BmiI GGNNCC 9 cut(s) 248, 249, 296, 338, 590, 617, 692, 768, 1241
BmrFI CCNGG 6 cut(s) 102, 207, 278, 473, 574, 1271
BmsI GCATC 6 cut(s) 49, 271, 940, 1011, 1095, 1116
BoxI GACNNNNGTC 1 cut(s) 1205
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 2 cut(s) 102, 922
Bpu14I TTCGAA 1 cut(s) 1127
BsaHI GRCGYC 1 cut(s) 616
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 6 cut(s) 100, 101, 340, 471, 472, 1203
BsaWI WCCGGW 1 cut(s) 758
Bse118I RCCGGY 2 cut(s) 662, 1232
Bse3DI GCAATG 1 cut(s) 345
BseBI CCWGG 6 cut(s) 102, 207, 278, 473, 574, 1271
BseDI CCNNGG 6 cut(s) 100, 101, 340, 471, 472, 1203
BseGI GGATG 3 cut(s) 137, 145, 385
BseMI GCAATG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 4 cut(s) 295, 479, 947, 1064
Bsh1236I CGCG 1 cut(s) 169
BshFI GGCC 1 cut(s) 66
BshNI GGYRCC 3 cut(s) 294, 588, 615
BsiHKAI GWGCWC 1 cut(s) 758
BsiSI CCGG 3 cut(s) 663, 759, 1233
BslFI GGGAC 4 cut(s) 83, 233, 434, 676
BsmAI GTCTC 2 cut(s) 400, 825
BsmFI GGGAC 4 cut(s) 83, 233, 434, 676
BsmI GAATGC 2 cut(s) 186, 953
BsnI GGCC 1 cut(s) 66
Bso31I GGTCTC 1 cut(s) 825
Bsp119I TTCGAA 1 cut(s) 1127
Bsp1286I GDGCHC 2 cut(s) 598, 758
Bsp143I GATC 4 cut(s) 220, 412, 542, 715
BspACI CCGC 6 cut(s) 184, 274, 533, 613, 666, 1243
BspANI GGCC 1 cut(s) 66
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 9 cut(s) 248, 249, 296, 338, 590, 617, 692, 768, 1241
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 1172
BspPI GGATC 3 cut(s) 407, 550, 710
BspT104I TTCGAA 1 cut(s) 1127
BspT107I GGYRCC 3 cut(s) 294, 588, 615
BspTNI GGTCTC 1 cut(s) 825
BsrDI GCAATG 1 cut(s) 345
BsrFI RCCGGY 2 cut(s) 662, 1232
BssAI RCCGGY 2 cut(s) 662, 1232
BssECI CCNNGG 6 cut(s) 100, 101, 340, 471, 472, 1203
BssMI GATC 4 cut(s) 220, 412, 542, 715
BssNAI GTATAC 1 cut(s) 421
BssNI GRCGYC 1 cut(s) 616
BssSI CACGAG 1 cut(s) 631
BssT1I CCWWGG 2 cut(s) 340, 1203
Bst1107I GTATAC 1 cut(s) 421
Bst2BI CACGAG 1 cut(s) 631
Bst2UI CCWGG 6 cut(s) 102, 207, 278, 473, 574, 1271
Bst4CI ACNGT 3 cut(s) 76, 299, 593
BstACI GRCGYC 1 cut(s) 616
BstAPI GCANNNNNTGC 1 cut(s) 1083
BstBI TTCGAA 1 cut(s) 1127
BstC8I GCNNGC 8 cut(s) 43, 64, 235, 405, 664, 898, 1004, 1234
BstDEI CTNAG 2 cut(s) 968, 1038
BstF5I GGATG 3 cut(s) 137, 145, 385
BstFNI CGCG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 1 cut(s) 618
BstKTI GATC 4 cut(s) 223, 415, 545, 718
BstMAI GTCTC 2 cut(s) 400, 825
BstMBI GATC 4 cut(s) 220, 412, 542, 715
BstMWI GCNNNNNNNGC 8 cut(s) 268, 282, 613, 957, 1049, 1083, 1143, 1242
BstNI CCWGG 6 cut(s) 102, 207, 278, 473, 574, 1271
BstNSI RCATGY 1 cut(s) 407
BstPAI GACNNNNGTC 1 cut(s) 1205
BstSCI CCNGG 6 cut(s) 100, 205, 276, 471, 572, 1269
BstSFI CTRYAG 2 cut(s) 465, 1176
BstSLI GKGCMC 1 cut(s) 598
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 4 cut(s) 295, 479, 947, 1064
BstV2I GAAGAC 1 cut(s) 110
BstXI CCANNNNNNTGG 1 cut(s) 626
BstZ17I GTATAC 1 cut(s) 421
BsuI GTATCC 1 cut(s) 6
BsuRI GGCC 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 1152
BtsCI GGATG 3 cut(s) 137, 145, 385
BtsI GCAGTG 1 cut(s) 1264
BtsIMutI CAGTG 1 cut(s) 1264
BveI ACCTGC 1 cut(s) 1172
Cac8I GCNNGC 8 cut(s) 43, 64, 235, 405, 664, 898, 1004, 1234
CfoI GCGC 1 cut(s) 618
Cfr10I RCCGGY 2 cut(s) 662, 1232
Cfr13I GGNCC 5 cut(s) 247, 576, 690, 735, 1200
Csp6I GTAC 3 cut(s) 295, 491, 871
CviAII CATG 2 cut(s) 404, 1102
CviQI GTAC 3 cut(s) 295, 491, 871
DdeI CTNAG 2 cut(s) 968, 1038
DinI GGCGCC 1 cut(s) 617
DpnI GATC 4 cut(s) 222, 414, 544, 717
DpnII GATC 4 cut(s) 220, 412, 542, 715
DraI TTTAAA 1 cut(s) 924
EciI GGCGGA 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 340, 1203
Eco31I GGTCTC 1 cut(s) 825
Eco47I GGWCC 5 cut(s) 247, 576, 690, 735, 1200
Eco57I CTGAAG 1 cut(s) 726
EcoO109I RGGNCCY 2 cut(s) 247, 1200
EcoRII CCWGG 6 cut(s) 100, 205, 276, 471, 572, 1269
EcoT14I CCWWGG 2 cut(s) 340, 1203
EcoT22I ATGCAT 1 cut(s) 1026
EgeI GGCGCC 1 cut(s) 617
EheI GGCGCC 1 cut(s) 617
ErhI CCWWGG 2 cut(s) 340, 1203
FaeI CATG 2 cut(s) 407, 1105
FalI AAGNNNNNCTT 2 cut(s) 904, 936
FaqI GGGAC 4 cut(s) 83, 233, 434, 676
FatI CATG 2 cut(s) 403, 1101
FauI CCCGC 2 cut(s) 540, 659
FblI GTMKAC 2 cut(s) 420, 846
Fnu4HI GCNGC 6 cut(s) 184, 309, 468, 614, 961, 1053
FokI GGATG 3 cut(s) 124, 132, 392
Fsp4HI GCNGC 6 cut(s) 184, 309, 468, 614, 961, 1053
FspBI CTAG 2 cut(s) 153, 239
GlaI GCGC 1 cut(s) 617
GluI GCNGC 6 cut(s) 184, 309, 468, 614, 961, 1053
GsuI CTGGAG 2 cut(s) 102, 922
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 1 cut(s) 66
HapII CCGG 3 cut(s) 663, 759, 1233
HhaI GCGC 1 cut(s) 618
Hin1I GRCGYC 1 cut(s) 616
Hin1II CATG 2 cut(s) 407, 1105
Hin6I GCGC 1 cut(s) 616
HinP1I GCGC 1 cut(s) 616
HinfI GANTC 6 cut(s) 32, 210, 555, 907, 992, 1213
HpaII CCGG 3 cut(s) 663, 759, 1233
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 4 cut(s) 421, 787, 847, 1264
Hpy188I TCNGA 3 cut(s) 748, 946, 1095
Hpy188III TCNNGA 3 cut(s) 805, 1198, 1210
Hpy8I GTNNAC 4 cut(s) 421, 787, 847, 1264
Hpy99I CGWCG 1 cut(s) 991
HpyAV CCTTC 6 cut(s) 3, 174, 380, 748, 785, 802
HpyCH4III ACNGT 3 cut(s) 76, 299, 593
HpyCH4V TGCA 8 cut(s) 285, 350, 467, 896, 951, 963, 1024, 1086
HpyF10VI GCNNNNNNNGC 8 cut(s) 268, 282, 613, 957, 1049, 1083, 1143, 1242
HpyF3I CTNAG 2 cut(s) 968, 1038
Hsp92I GRCGYC 1 cut(s) 616
Hsp92II CATG 2 cut(s) 407, 1105
HspAI GCGC 1 cut(s) 616
KasI GGCGCC 1 cut(s) 615
KflI GGGWCCC 1 cut(s) 247
KpnI GGTACC 1 cut(s) 298
KroI GCCGGC 2 cut(s) 662, 1232
KroNI GCCGGC 2 cut(s) 664, 1234
Kzo9I GATC 4 cut(s) 220, 412, 542, 715
LmnI GCTCC 4 cut(s) 83, 276, 772, 999
Lsp1109I GCAGC 4 cut(s) 295, 479, 947, 1064
LweI GCATC 6 cut(s) 49, 271, 940, 1011, 1095, 1116
MaeI CTAG 2 cut(s) 153, 239
MaeIII GTNAC 3 cut(s) 503, 1207, 1253
MalI GATC 4 cut(s) 222, 414, 544, 717
MboI GATC 4 cut(s) 220, 412, 542, 715
MboII GAAGA 3 cut(s) 110, 365, 1268
MhlI GDGCHC 2 cut(s) 598, 758
MluCI AATT 2 cut(s) 369, 1033
Mly113I GGCGCC 1 cut(s) 616
MlyI GAGTC 2 cut(s) 204, 564
MmeI TCCRAC 1 cut(s) 173
Mph1103I ATGCAT 1 cut(s) 1026
MroNI GCCGGC 2 cut(s) 662, 1232
MseI TTAA 6 cut(s) 69, 132, 368, 372, 923, 1248
MslI CAYNNNNRTG 2 cut(s) 761, 936
MspA1I CMGCKG 2 cut(s) 704, 867
MspI CCGG 3 cut(s) 663, 759, 1233
MspR9I CCNGG 6 cut(s) 102, 207, 278, 473, 574, 1271
Mva1269I GAATGC 2 cut(s) 186, 953
MvaI CCWGG 6 cut(s) 102, 207, 278, 473, 574, 1271
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 8 cut(s) 268, 282, 613, 957, 1049, 1083, 1143, 1242
NaeI GCCGGC 2 cut(s) 664, 1234
NarI GGCGCC 1 cut(s) 616
NdeII GATC 4 cut(s) 220, 412, 542, 715
NgoMIV GCCGGC 2 cut(s) 662, 1232
NlaIII CATG 2 cut(s) 407, 1105
NlaIV GGNNCC 9 cut(s) 248, 249, 296, 338, 590, 617, 692, 768, 1241
NmuCI GTSAC 3 cut(s) 503, 1207, 1253
NsiI ATGCAT 1 cut(s) 1026
NspI RCATGY 1 cut(s) 407
NspV TTCGAA 1 cut(s) 1127
PacI TTAATTAA 1 cut(s) 372
PaeI GCATGC 1 cut(s) 407
PasI CCCWGGG 2 cut(s) 101, 472
PctI GAATGC 2 cut(s) 186, 953
PdiI GCCGGC 2 cut(s) 664, 1234
PfeI GAWTC 4 cut(s) 32, 907, 992, 1213
PflMI CCANNNNNTGG 1 cut(s) 585
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 6 cut(s) 185, 310, 469, 615, 962, 1054
PleI GAGTC 2 cut(s) 204, 563
PluTI GGCGCC 1 cut(s) 619
PpsI GAGTC 2 cut(s) 204, 563
PpuMI RGGWCCY 2 cut(s) 247, 1200
PshAI GACNNNNGTC 1 cut(s) 1205
PsiI TTATAA 2 cut(s) 567, 641
Psp5II RGGWCCY 2 cut(s) 247, 1200
Psp6I CCWGG 6 cut(s) 100, 205, 276, 471, 572, 1269
PspGI CCWGG 6 cut(s) 100, 205, 276, 471, 572, 1269
PspN4I GGNNCC 9 cut(s) 248, 249, 296, 338, 590, 617, 692, 768, 1241
PspPI GGNCC 5 cut(s) 247, 576, 690, 735, 1200
PspPPI RGGWCCY 2 cut(s) 247, 1200
PstI CTGCAG 1 cut(s) 469
PvuII CAGCTG 2 cut(s) 704, 867
RsaI GTAC 3 cut(s) 296, 492, 872
RsaNI GTAC 3 cut(s) 295, 491, 871
RseI CAYNNNNRTG 2 cut(s) 761, 936
SaqAI TTAA 6 cut(s) 69, 132, 368, 372, 923, 1248
SatI GCNGC 6 cut(s) 184, 309, 468, 614, 961, 1053
Sau3AI GATC 4 cut(s) 220, 412, 542, 715
Sau96I GGNCC 5 cut(s) 247, 576, 690, 735, 1200
SchI GAGTC 2 cut(s) 204, 564
ScrFI CCNGG 6 cut(s) 102, 207, 278, 473, 574, 1271
SduI GDGCHC 2 cut(s) 598, 758
SfaNI GCATC 6 cut(s) 49, 271, 940, 1011, 1095, 1116
SfcI CTRYAG 2 cut(s) 465, 1176
SfoI GGCGCC 1 cut(s) 617
SfuI TTCGAA 1 cut(s) 1127
SinI GGWCC 5 cut(s) 247, 576, 690, 735, 1200
SmiMI CAYNNNNRTG 2 cut(s) 761, 936
SphI GCATGC 1 cut(s) 407
Sse9I AATT 2 cut(s) 369, 1033
SsiI CCGC 6 cut(s) 184, 274, 533, 613, 666, 1243
SspDI GGCGCC 1 cut(s) 615
SspI AATATT 1 cut(s) 461
SspMI CTAG 2 cut(s) 153, 239
StyD4I CCNGG 6 cut(s) 100, 205, 276, 471, 572, 1269
StyI CCWWGG 2 cut(s) 340, 1203
TaaI ACNGT 3 cut(s) 76, 299, 593
TaqI TCGA 2 cut(s) 826, 1127
TaqII GACCGA 1 cut(s) 678
TasI AATT 2 cut(s) 369, 1033
TatI WGTACW 1 cut(s) 490
TauI GCSGC 2 cut(s) 186, 616
TfiI GAWTC 4 cut(s) 32, 907, 992, 1213
Tru1I TTAA 6 cut(s) 69, 132, 368, 372, 923, 1248
Tru9I TTAA 6 cut(s) 69, 132, 368, 372, 923, 1248
TscAI CASTG 1 cut(s) 1271
TseFI GTSAC 3 cut(s) 503, 1207, 1253
TseI GCWGC 4 cut(s) 308, 467, 960, 1052
Tsp45I GTSAC 3 cut(s) 503, 1207, 1253
TspDTI ATGAA 2 cut(s) 134, 730
TspGWI ACGGA 4 cut(s) 12, 320, 900, 1004
TspRI CASTG 1 cut(s) 1271
Van91I CCANNNNNTGG 1 cut(s) 585
VpaK11BI GGWCC 5 cut(s) 247, 576, 690, 735, 1200
XceI RCATGY 1 cut(s) 407
XmiI GTMKAC 2 cut(s) 420, 846
XspI CTAG 2 cut(s) 153, 239
Zsp2I ATGCAT 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.