Rh2BG040800

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002477
Physical Location & Seq
Reverse (-)
2829803 .. 2833337
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG040800.1

Sequence Viewer

Length: 1293 bp
ATGGACAGAAGAAGAATGATACAGCCTCCATTGGTGGATAGCACAGCATGTTTATGCAGAGTTGATGCTGGTCTTAAAACTGTTGCTGGAGCAAAAAAGTATGTCCCTGGGGCAAAGCTTTGTCTGCAACCAGACATTAAAGCATCCATTCATCCAACTAGAAGTAAGCCTGCACGTGGTGAGAGTAGCCGCAATCAATCCCCTCTTCTTCCTGGACTCCCTGATGATCTTGCTATTGCTTGCCTAATTCGGGTCCCAAGAGTGGAGCATCGCAAGCTCCGCCTAGTTTGCAAAAGATGGTACCGTCTTCTGGCTGGCAACTTCTTTTACTCCCTCCGTAAGAGCATTGGCATTGCTGAAGAATGGATATACTTGATTAAAAGAGATCGAGATGGGAAAATATCTTGGCATGCCTTTGACCCTGTATATCAGCTATGGCAGTCCCTTCCTCCTGTTCCTAAGGAATATTCTGAAGCTCTTGGATTTGGTTGTGCTGTACTCGGTGGCTGTCACCTCTATCTGTTTGGAGGAAAGGACCCTCTAAAGGGATCAATGAGACGAGTCATTTTTTACAGTGCCAGGACCAATAAATGGCATCGTGCCCCAGATATGCTTCGCAGACGACATTTTTTTGGCTCGTGTGTTATAAATAACTGCCTCTATGTTGCTGGTGGAGAGAATGAGGGGGTGCATAGGTCCTTGAGATCAGCAGAAGTCTATGATCCCAACAAGAATAGATGGTCCTTCATTTCAGATATGAGCACTGCAATGGTGCCTTTCATCGGGGTTGTCTACGAAGGCAAATGGTTCTTGAAGGGGCTTGGGTCTCATCGACAAGTCCTGAGTGAGGTCTATCAACCAGAAAATGACAGCTGGTACTCTGTGTATGACGGAATGGTTGCAGGCTGGAGGAACCCCAGTGCTTCTTTAAATGGGCATCTCTATGCTTTGGAGTGCAAGGATGGCTGCAAACTCAGAGTTTACGATGAAGCAACTGATTCTTGGAGCAAGCATATCGACAGTAAGATACATTTGGGTAACTCTAGGGCCTTGGAGGCAGCTTCCCTGGTTCCCCTTAACGGTAAACTTTGCATCATCCGAAATAATATGAGTATTTCGCTTGTTGATGTTTCGAAATCAAATGATGCTGGTGGAGCAAGTGCTCAACATCTATGGGAAACTCTAGCAGGCAAAGGGCAGTTCAGGACAATGGTCACAAATCTGTGGTCAAGCCTTGCCGGCAGAAACCGCCTCAAAAGTCACATAGTTCACTGCCAGGTTCTTCAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

430

Amino Acids

48.36

Weight (kDa)

9.58

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 71 - 107 3.9e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 135 - 295 6.5e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 144 - 258 2.3e-10 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 149 - 337 1.1e-19 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 182 - 293 2.6e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 207 - 254 3.6e-15 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
Acc65I GGTACC 1 cut(s) 300
AccB1I GGYRCC 2 cut(s) 300, 772
AccB7I CCANNNNNTGG 1 cut(s) 591
AccI GTMKAC 1 cut(s) 792
AciI CCGC 3 cut(s) 190, 280, 1249
AclWI GGATC 2 cut(s) 556, 716
AcuI CTGAAG 2 cut(s) 378, 492
AcvI CACGTG 1 cut(s) 176
AdeI CACNNNGTG 1 cut(s) 179
AfaI GTAC 3 cut(s) 302, 498, 878
AgsI TTSAA 2 cut(s) 814, 1286
AjnI CCWGG 5 cut(s) 106, 211, 578, 1065, 1275
AluBI AGCT 7 cut(s) 118, 277, 433, 476, 873, 1061, 1289
AluI AGCT 7 cut(s) 118, 277, 433, 476, 873, 1061, 1289
Alw21I GWGCWC 2 cut(s) 764, 1165
Alw26I GTCTC 2 cut(s) 550, 831
AlwI GGATC 2 cut(s) 556, 716
AoxI GGCC 1 cut(s) 1047
ApeKI GCWGC 2 cut(s) 966, 1058
ArsI GACNNNNNNTTYG 2 cut(s) 1211, 1243
Asp718I GGTACC 1 cut(s) 300
AspS9I GGNCC 6 cut(s) 253, 535, 582, 696, 741, 1047
AsuHPI GGTGA 2 cut(s) 191, 503
AsuII TTCGAA 1 cut(s) 1133
AvaII GGWCC 5 cut(s) 253, 535, 582, 696, 741
AxyI CCTNAGG 1 cut(s) 459
BaeGI GKGCMC 1 cut(s) 604
BanI GGYRCC 2 cut(s) 300, 772
BauI CACGAG 1 cut(s) 637
BbrPI CACGTG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 764, 1165
BbvI GCAGC 2 cut(s) 953, 1070
BccI CCATC 4 cut(s) 291, 386, 732, 956
BcgI CGANNNNNNTGC 2 cut(s) 997, 1031
BciT130I CCWGG 5 cut(s) 108, 213, 580, 1067, 1277
BcoDI GTCTC 2 cut(s) 550, 831
BfaI CTAG 4 cut(s) 159, 284, 1044, 1184
BglI GCCNNNNNGGC 2 cut(s) 1055, 1239
BisI GCNGC 3 cut(s) 190, 967, 1059
BlsI GCNGC 3 cut(s) 191, 968, 1060
Bme1390I CCNGG 5 cut(s) 108, 213, 580, 1067, 1277
Bme18I GGWCC 5 cut(s) 253, 535, 582, 696, 741
BmgT120I GGNCC 6 cut(s) 253, 535, 582, 696, 741, 1047
BmiI GGNNCC 7 cut(s) 254, 255, 302, 537, 774, 914, 1071
BmrFI CCNGG 5 cut(s) 108, 213, 580, 1067, 1277
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 7 cut(s) 55, 152, 277, 604, 946, 1101, 1135
BmuI ACTGGG 1 cut(s) 912
BoxI GACNNNNGTC 1 cut(s) 1211
BpiI GAAGAC 1 cut(s) 299
BpmI CTGGAG 2 cut(s) 108, 928
Bpu14I TTCGAA 1 cut(s) 1133
BpuEI CTTGAG 1 cut(s) 721
BsaAI YACGTR 1 cut(s) 176
BsaI GGTCTC 1 cut(s) 831
BsaJI CCNNGG 4 cut(s) 106, 107, 1050, 1065
Bse118I RCCGGY 1 cut(s) 1238
Bse1I ACTGG 1 cut(s) 918
Bse21I CCTNAGG 1 cut(s) 459
Bse3DI GCAATG 2 cut(s) 351, 774
BseBI CCWGG 5 cut(s) 108, 213, 580, 1067, 1277
BseDI CCNNGG 4 cut(s) 106, 107, 1050, 1065
BseGI GGATG 4 cut(s) 143, 151, 967, 1095
BseMI GCAATG 2 cut(s) 351, 774
BseMII CTCAG 2 cut(s) 833, 988
BseNI ACTGG 1 cut(s) 918
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 2 cut(s) 953, 1070
BsgI GTGCAG 1 cut(s) 156
BshFI GGCC 1 cut(s) 1049
BshNI GGYRCC 2 cut(s) 300, 772
BsiHKAI GWGCWC 2 cut(s) 764, 1165
BsiSI CCGG 1 cut(s) 1239
BslFI GGGAC 3 cut(s) 89, 239, 427
BsmAI GTCTC 2 cut(s) 550, 831
BsmBI CGTCTC 1 cut(s) 550
BsmFI GGGAC 3 cut(s) 89, 239, 427
BsnI GGCC 1 cut(s) 1049
Bso31I GGTCTC 1 cut(s) 831
Bsp119I TTCGAA 1 cut(s) 1133
Bsp1286I GDGCHC 3 cut(s) 604, 764, 1165
Bsp143I GATC 5 cut(s) 226, 385, 548, 704, 721
BspACI CCGC 3 cut(s) 190, 280, 1249
BspANI GGCC 1 cut(s) 1049
BspCNI CTCAG 2 cut(s) 834, 987
BspLI GGNNCC 7 cut(s) 254, 255, 302, 537, 774, 914, 1071
BspPI GGATC 2 cut(s) 556, 716
BspT104I TTCGAA 1 cut(s) 1133
BspT107I GGYRCC 2 cut(s) 300, 772
BspTNI GGTCTC 1 cut(s) 831
BsrDI GCAATG 2 cut(s) 351, 774
BsrFI RCCGGY 1 cut(s) 1238
BsrI ACTGG 1 cut(s) 918
BssAI RCCGGY 1 cut(s) 1238
BssECI CCNNGG 4 cut(s) 106, 107, 1050, 1065
BssMI GATC 5 cut(s) 226, 385, 548, 704, 721
BssSI CACGAG 1 cut(s) 637
BssT1I CCWWGG 1 cut(s) 1050
Bst2BI CACGAG 1 cut(s) 637
Bst2UI CCWGG 5 cut(s) 108, 213, 580, 1067, 1277
Bst4CI ACNGT 5 cut(s) 82, 305, 575, 1022, 1082
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 176
BstBI TTCGAA 1 cut(s) 1133
BstC8I GCNNGC 9 cut(s) 171, 241, 275, 316, 411, 904, 1010, 1189, 1240
BstDEI CTNAG 4 cut(s) 459, 842, 974, 1290
BstENI CCTNNNNNAGG 1 cut(s) 845
BstF5I GGATG 4 cut(s) 143, 151, 967, 1095
BstKTI GATC 5 cut(s) 229, 388, 551, 707, 724
BstMAI GTCTC 2 cut(s) 550, 831
BstMBI GATC 5 cut(s) 226, 385, 548, 704, 721
BstMWI GCNNNNNNNGC 9 cut(s) 124, 274, 279, 288, 963, 1055, 1154, 1239, 1248
BstNI CCWGG 5 cut(s) 108, 213, 580, 1067, 1277
BstNSI RCATGY 2 cut(s) 51, 413
BstPAI GACNNNNGTC 1 cut(s) 1211
BstSCI CCNGG 5 cut(s) 106, 211, 578, 1065, 1275
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 2 cut(s) 953, 1070
BstV2I GAAGAC 1 cut(s) 299
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 1 cut(s) 1049
BtgZI GCGATG 1 cut(s) 254
BtsCI GGATG 4 cut(s) 143, 151, 967, 1095
BtsI GCAGTG 2 cut(s) 762, 1270
BtsIMutI CAGTG 4 cut(s) 580, 762, 925, 1270
Cac8I GCNNGC 9 cut(s) 171, 241, 275, 316, 411, 904, 1010, 1189, 1240
Cfr10I RCCGGY 1 cut(s) 1238
Cfr13I GGNCC 6 cut(s) 253, 535, 582, 696, 741, 1047
Csp6I GTAC 3 cut(s) 301, 497, 877
CviAII CATG 2 cut(s) 48, 410
CviQI GTAC 3 cut(s) 301, 497, 877
DdeI CTNAG 4 cut(s) 459, 842, 974, 1290
DpnI GATC 5 cut(s) 228, 387, 550, 706, 723
DpnII GATC 5 cut(s) 226, 385, 548, 704, 721
DraI TTTAAA 1 cut(s) 930
DraIII CACNNNGTG 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 1050
Eco31I GGTCTC 1 cut(s) 831
Eco47I GGWCC 5 cut(s) 253, 535, 582, 696, 741
Eco57I CTGAAG 2 cut(s) 378, 492
Eco72I CACGTG 1 cut(s) 176
Eco81I CCTNAGG 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 845
EcoO109I RGGNCCY 4 cut(s) 253, 535, 696, 1047
EcoRII CCWGG 5 cut(s) 106, 211, 578, 1065, 1275
EcoT14I CCWWGG 1 cut(s) 1050
ErhI CCWWGG 1 cut(s) 1050
Esp3I CGTCTC 1 cut(s) 550
FaeI CATG 2 cut(s) 51, 413
FaqI GGGAC 3 cut(s) 89, 239, 427
FatI CATG 2 cut(s) 47, 409
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 3 cut(s) 190, 967, 1059
FokI GGATG 4 cut(s) 130, 138, 974, 1082
Fsp4HI GCNGC 3 cut(s) 190, 967, 1059
FspBI CTAG 4 cut(s) 159, 284, 1044, 1184
GluI GCNGC 3 cut(s) 190, 967, 1059
GsuI CTGGAG 2 cut(s) 108, 928
HaeIII GGCC 1 cut(s) 1049
HapII CCGG 1 cut(s) 1239
Hin1II CATG 2 cut(s) 51, 413
HindIII AAGCTT 2 cut(s) 116, 1287
HinfI GANTC 3 cut(s) 216, 561, 998
HpaII CCGG 1 cut(s) 1239
HphI GGTGA 2 cut(s) 191, 503
Hpy166II GTNNAC 4 cut(s) 793, 982, 1085, 1270
Hpy188I TCNGA 4 cut(s) 472, 754, 977, 1100
Hpy188III TCNNGA 4 cut(s) 389, 811, 841, 1204
Hpy8I GTNNAC 4 cut(s) 793, 982, 1085, 1270
HpyAV CCTTC 4 cut(s) 455, 754, 791, 808
HpyCH4III ACNGT 5 cut(s) 82, 305, 575, 1022, 1082
HpyCH4IV ACGT 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 9 cut(s) 124, 274, 279, 288, 963, 1055, 1154, 1239, 1248
HpyF3I CTNAG 4 cut(s) 459, 842, 974, 1290
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 2 cut(s) 51, 413
KflI GGGWCCC 1 cut(s) 253
KpnI GGTACC 1 cut(s) 304
KroI GCCGGC 1 cut(s) 1238
KroNI GCCGGC 1 cut(s) 1240
Kzo9I GATC 5 cut(s) 226, 385, 548, 704, 721
LmnI GCTCC 5 cut(s) 89, 265, 282, 1005, 1154
Lsp1109I GCAGC 2 cut(s) 953, 1070
LweI GCATC 7 cut(s) 55, 152, 277, 604, 946, 1101, 1135
MaeI CTAG 4 cut(s) 159, 284, 1044, 1184
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 4 cut(s) 509, 1037, 1213, 1259
MalI GATC 5 cut(s) 228, 387, 550, 706, 723
MboI GATC 5 cut(s) 226, 385, 548, 704, 721
MboII GAAGA 7 cut(s) 21, 24, 197, 200, 299, 371, 1274
MhlI GDGCHC 3 cut(s) 604, 764, 1165
MluCI AATT 1 cut(s) 246
MlyI GAGTC 2 cut(s) 210, 570
MmeI TCCRAC 1 cut(s) 179
MroNI GCCGGC 1 cut(s) 1238
MseI TTAA 5 cut(s) 75, 138, 378, 929, 1077
MslI CAYNNNNRTG 3 cut(s) 52, 767, 942
MspA1I CMGCKG 1 cut(s) 873
MspI CCGG 1 cut(s) 1239
MspR9I CCNGG 5 cut(s) 108, 213, 580, 1067, 1277
MvaI CCWGG 5 cut(s) 108, 213, 580, 1067, 1277
MwoI GCNNNNNNNGC 9 cut(s) 124, 274, 279, 288, 963, 1055, 1154, 1239, 1248
NaeI GCCGGC 1 cut(s) 1240
NdeII GATC 5 cut(s) 226, 385, 548, 704, 721
NgoMIV GCCGGC 1 cut(s) 1238
NlaIII CATG 2 cut(s) 51, 413
NlaIV GGNNCC 7 cut(s) 254, 255, 302, 537, 774, 914, 1071
NmuCI GTSAC 3 cut(s) 509, 1213, 1259
NspI RCATGY 2 cut(s) 51, 413
NspV TTCGAA 1 cut(s) 1133
PaeI GCATGC 1 cut(s) 413
PasI CCCWGGG 1 cut(s) 107
PdiI GCCGGC 1 cut(s) 1240
PfeI GAWTC 1 cut(s) 998
PflMI CCANNNNNTGG 1 cut(s) 591
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 3 cut(s) 191, 968, 1060
PleI GAGTC 2 cut(s) 210, 569
PmaCI CACGTG 1 cut(s) 176
PmlI CACGTG 1 cut(s) 176
PpsI GAGTC 2 cut(s) 210, 569
Ppu21I YACGTR 1 cut(s) 176
PpuMI RGGWCCY 3 cut(s) 253, 535, 696
PshAI GACNNNNGTC 1 cut(s) 1211
PsiI TTATAA 1 cut(s) 647
Psp5II RGGWCCY 3 cut(s) 253, 535, 696
Psp6I CCWGG 5 cut(s) 106, 211, 578, 1065, 1275
PspCI CACGTG 1 cut(s) 176
PspGI CCWGG 5 cut(s) 106, 211, 578, 1065, 1275
PspN4I GGNNCC 7 cut(s) 254, 255, 302, 537, 774, 914, 1071
PspPI GGNCC 6 cut(s) 253, 535, 582, 696, 741, 1047
PspPPI RGGWCCY 3 cut(s) 253, 535, 696
PvuII CAGCTG 1 cut(s) 873
RsaI GTAC 3 cut(s) 302, 498, 878
RsaNI GTAC 3 cut(s) 301, 497, 877
RseI CAYNNNNRTG 3 cut(s) 52, 767, 942
SaqAI TTAA 5 cut(s) 75, 138, 378, 929, 1077
SatI GCNGC 3 cut(s) 190, 967, 1059
Sau3AI GATC 5 cut(s) 226, 385, 548, 704, 721
Sau96I GGNCC 6 cut(s) 253, 535, 582, 696, 741, 1047
SchI GAGTC 2 cut(s) 210, 570
ScrFI CCNGG 5 cut(s) 108, 213, 580, 1067, 1277
SduI GDGCHC 3 cut(s) 604, 764, 1165
SfaNI GCATC 7 cut(s) 55, 152, 277, 604, 946, 1101, 1135
SfuI TTCGAA 1 cut(s) 1133
SinI GGWCC 5 cut(s) 253, 535, 582, 696, 741
SmiMI CAYNNNNRTG 3 cut(s) 52, 767, 942
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
SphI GCATGC 1 cut(s) 413
Sse9I AATT 1 cut(s) 246
SsiI CCGC 3 cut(s) 190, 280, 1249
SspI AATATT 1 cut(s) 467
SspMI CTAG 4 cut(s) 159, 284, 1044, 1184
StyD4I CCNGG 5 cut(s) 106, 211, 578, 1065, 1275
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 5 cut(s) 82, 305, 575, 1022, 1082
TaiI ACGT 1 cut(s) 178
TaqI TCGA 4 cut(s) 388, 832, 1017, 1133
TasI AATT 1 cut(s) 246
TatI WGTACW 1 cut(s) 496
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 1 cut(s) 998
Tru1I TTAA 5 cut(s) 75, 138, 378, 929, 1077
Tru9I TTAA 5 cut(s) 75, 138, 378, 929, 1077
TscAI CASTG 4 cut(s) 580, 769, 925, 1277
TseFI GTSAC 3 cut(s) 509, 1213, 1259
TseI GCWGC 2 cut(s) 966, 1058
Tsp45I GTSAC 3 cut(s) 509, 1213, 1259
TspDTI ATGAA 4 cut(s) 140, 736, 769, 1002
TspGWI ACGGA 2 cut(s) 326, 906
TspRI CASTG 4 cut(s) 580, 769, 925, 1277
Van91I CCANNNNNTGG 1 cut(s) 591
VpaK11BI GGWCC 5 cut(s) 253, 535, 582, 696, 741
XagI CCTNNNNNAGG 1 cut(s) 845
XceI RCATGY 2 cut(s) 51, 413
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 4 cut(s) 159, 284, 1044, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.