Rh5AG529500

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002477
Physical Location & Seq
Reverse (-)
90896994 .. 90907071
10078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG529500.1

Sequence Viewer

Length: 809 bp
ATGGCAGAAGAGTGGGTTTATGTCATCAAAAGAGACCGCGATGGAAGAATCTCTTGGCATGCTTTTGATCCCACGTACCAGCTCTGGCAGCCACTTCCGACCGTTCCTGGTGAATATTCTGCAGCGCTTGGTTTTGGTTGTGCTGTCCTTAGTGGTTGCCACCTGTACTTATTTGGAGGAAAAGATCCATTGAAGGGATCTATGAGACGGGTTATTTTTTACAGTGCTCGGACAAATAAATGGCACAGGGCACCAGATATGCTTCGAAAATGGCATTTCTTTGGTGCTTGTGTTATTAATAACTGCCTTTATGTAGCTGGTGGAGAGTGTGAAGGAATACAAAGGACTCTCCGTTCTGCTGAAATTTATGACCCGAACAAGAACCGGTGGAGCTTTATTTCAGATATGAGCACAGCTATGGTTCCCTTTATTGGGGTTGTTCACGATGGTATGTGGTTTCTAAAAGGACTTGGATCGCACCATGAGGTAATGAGTGAAGCCTATTCTCCTGAAGCTAACACTTGGAACCCCATTAGTGATGGGATGGTTGCTGGTTGGCGCAACCCAAGTATTTCTTTAAATGGGCAACTCTATGCTCTGGATTACCGTGATGGGTGCAAGGACTTCATTTTCAACTGTGGGGGTTCATCAGTGGCCTTCATCTTTGTCACAAGCTTGGATTCAACTAATGTCTCCCAAATCTTCGGCAGAACTCAAATACTGAAGCAGCAATTTGCACATCTTTATGTTATCTGTACCCTCCCAACCAAAGAGCAAAATGACTTGTTTGTTCGTTCTTACTTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

269

Amino Acids

30.56

Weight (kDa)

7.6

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 18 - 176 1.7e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 46 - 206 1.8e-14 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 65 - 180 1.2e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 92 - 137 4.5e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AccII CGCG 1 cut(s) 39
AciI CCGC 1 cut(s) 37
AclWI GGATC 4 cut(s) 62, 179, 205, 481
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 2 cut(s) 531, 743
AfaI GTAC 3 cut(s) 77, 167, 757
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 3 cut(s) 194, 431, 432
AgeI ACCGGT 1 cut(s) 384
AgsI TTSAA 4 cut(s) 193, 634, 684, 805
AjnI CCWGG 1 cut(s) 106
AjuI GAANNNNNNNTTGG 2 cut(s) 37, 69
AloI GAACNNNNNNTCC 2 cut(s) 337, 369
AluBI AGCT 6 cut(s) 82, 317, 393, 416, 515, 675
AluI AGCT 6 cut(s) 82, 317, 393, 416, 515, 675
Alw21I GWGCWC 2 cut(s) 229, 413
Alw26I GTCTC 3 cut(s) 27, 199, 697
AlwI GGATC 4 cut(s) 62, 179, 205, 481
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 3 cut(s) 88, 122, 727
ApoI RAATTY 1 cut(s) 363
AseI ATTAAT 1 cut(s) 297
AsiGI ACCGGT 1 cut(s) 384
AspLEI GCGC 2 cut(s) 127, 561
AsuHPI GGTGA 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 265
BaeGI GKGCMC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 229, 413
BbvI GCAGC 3 cut(s) 100, 134, 739
BccI CCATC 5 cut(s) 35, 440, 533, 538, 605
BciT130I CCWGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 27, 199, 697
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 128
BisI GCNGC 3 cut(s) 89, 123, 728
BlsI GCNGC 3 cut(s) 90, 124, 729
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 3 cut(s) 252, 423, 527
BmrFI CCNGG 1 cut(s) 108
Bpu14I TTCGAA 1 cut(s) 265
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 384
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 431, 432
Bse118I RCCGGY 1 cut(s) 384
BseBI CCWGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 549
BseLI CCNNNNNNNGG 3 cut(s) 194, 431, 432
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 3 cut(s) 100, 134, 739
Bsh1236I CGCG 1 cut(s) 39
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 656
BshNI GGYRCC 1 cut(s) 250
BshTI ACCGGT 1 cut(s) 384
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 2 cut(s) 229, 413
BsiSI CCGG 1 cut(s) 385
BslI CCNNNNNNNGG 3 cut(s) 194, 431, 432
BsmAI GTCTC 3 cut(s) 27, 199, 697
BsmBI CGTCTC 1 cut(s) 199
BsnI GGCC 1 cut(s) 656
Bso31I GGTCTC 1 cut(s) 27
Bsp119I TTCGAA 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 229, 253, 413
Bsp143I GATC 4 cut(s) 67, 184, 197, 473
BspACI CCGC 1 cut(s) 37
BspANI GGCC 1 cut(s) 656
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 252, 423, 527
BspMAI CTGCAG 1 cut(s) 124
BspPI GGATC 4 cut(s) 62, 179, 205, 481
BspT104I TTCGAA 1 cut(s) 265
BspT107I GGYRCC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 384
BssAI RCCGGY 1 cut(s) 384
BssMI GATC 4 cut(s) 67, 184, 197, 473
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 4 cut(s) 103, 224, 608, 638
Bst6I CTCTTC 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 549
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 2 cut(s) 127, 561
BstKTI GATC 4 cut(s) 70, 187, 200, 476
BstMAI GTCTC 3 cut(s) 27, 199, 697
BstMBI GATC 4 cut(s) 67, 184, 197, 473
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 100, 134, 739
BstX2I RGATCY 2 cut(s) 184, 197
BstYI RGATCY 2 cut(s) 184, 197
BsuRI GGCC 1 cut(s) 656
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 549
BtsIMutI CAGTG 2 cut(s) 229, 657
Cac8I GCNNGC 1 cut(s) 60
CfoI GCGC 2 cut(s) 127, 561
Cfr10I RCCGGY 1 cut(s) 384
Csp6I GTAC 3 cut(s) 76, 166, 756
CspAI ACCGGT 1 cut(s) 384
CviAII CATG 2 cut(s) 59, 482
CviJI RGCY 9 cut(s) 82, 91, 317, 393, 416, 500, 515, 656, 675
CviKI_1 RGCY 9 cut(s) 82, 91, 317, 393, 416, 500, 515, 656, 675
CviQI GTAC 3 cut(s) 76, 166, 756
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 69, 186, 199, 475
DpnII GATC 4 cut(s) 67, 184, 197, 473
DraI TTTAAA 1 cut(s) 579
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 27
Eco47III AGCGCT 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 531, 743
EcoRII CCWGG 1 cut(s) 106
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 2 cut(s) 62, 485
FalI AAGNNNNNCTT 4 cut(s) 37, 69, 559, 591
FatI CATG 2 cut(s) 58, 481
Fnu4HI GCNGC 3 cut(s) 89, 123, 728
FokI GGATG 1 cut(s) 556
Fsp4HI GCNGC 3 cut(s) 89, 123, 728
GlaI GCGC 2 cut(s) 126, 560
GluI GCNGC 3 cut(s) 89, 123, 728
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 1 cut(s) 656
HapII CCGG 1 cut(s) 385
HhaI GCGC 2 cut(s) 127, 561
Hin1II CATG 2 cut(s) 62, 485
Hin6I GCGC 2 cut(s) 125, 559
HinP1I GCGC 2 cut(s) 125, 559
HindIII AAGCTT 1 cut(s) 673
HinfI GANTC 3 cut(s) 48, 346, 680
HpaII CCGG 1 cut(s) 385
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 3 cut(s) 99, 231, 403
Hpy188III TCNNGA 3 cut(s) 443, 509, 599
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 3 cut(s) 187, 326, 667
HpyCH4III ACNGT 4 cut(s) 103, 224, 608, 638
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 3 cut(s) 122, 618, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 2 cut(s) 62, 485
HspAI GCGC 2 cut(s) 125, 559
Kzo9I GATC 4 cut(s) 67, 184, 197, 473
LmnI GCTCC 1 cut(s) 390
Lsp1109I GCAGC 3 cut(s) 100, 134, 739
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 1 cut(s) 667
MalI GATC 4 cut(s) 69, 186, 199, 475
MboI GATC 4 cut(s) 67, 184, 197, 473
MboII GAAGA 3 cut(s) 20, 57, 694
MflI RGATCY 2 cut(s) 184, 197
MhlI GDGCHC 3 cut(s) 229, 253, 413
MluCI AATT 2 cut(s) 363, 731
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 3 cut(s) 170, 478, 770
MseI TTAA 2 cut(s) 297, 578
MslI CAYNNNNRTG 2 cut(s) 416, 744
MspI CCGG 1 cut(s) 385
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 88
NdeII GATC 4 cut(s) 67, 184, 197, 473
NlaIII CATG 2 cut(s) 62, 485
NlaIV GGNNCC 3 cut(s) 252, 423, 527
NmuCI GTSAC 1 cut(s) 667
NspI RCATGY 1 cut(s) 62
NspV TTCGAA 1 cut(s) 265
PaeI GCATGC 1 cut(s) 62
PfeI GAWTC 2 cut(s) 48, 680
PinAI ACCGGT 1 cut(s) 384
PkrI GCNGC 3 cut(s) 90, 124, 729
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Ppu21I YACGTR 1 cut(s) 75
PshBI ATTAAT 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 3 cut(s) 252, 423, 527
PstI CTGCAG 1 cut(s) 124
PsuI RGATCY 2 cut(s) 184, 197
RsaI GTAC 3 cut(s) 77, 167, 757
RsaNI GTAC 3 cut(s) 76, 166, 756
RseI CAYNNNNRTG 2 cut(s) 416, 744
SaqAI TTAA 2 cut(s) 297, 578
SatI GCNGC 3 cut(s) 89, 123, 728
Sau3AI GATC 4 cut(s) 67, 184, 197, 473
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 3 cut(s) 229, 253, 413
SetI ASST 9 cut(s) 77, 84, 165, 319, 395, 418, 489, 517, 677
SfcI CTRYAG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 416, 744
SphI GCATGC 1 cut(s) 62
Sse9I AATT 2 cut(s) 363, 731
SsiI CCGC 1 cut(s) 37
SspI AATATT 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 4 cut(s) 103, 224, 608, 638
TaiI ACGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 265
TasI AATT 2 cut(s) 363, 731
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 2 cut(s) 48, 680
Tru1I TTAA 2 cut(s) 297, 578
Tru9I TTAA 2 cut(s) 297, 578
TscAI CASTG 2 cut(s) 229, 657
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 3 cut(s) 88, 122, 727
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 3 cut(s) 616, 636, 649
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 2 cut(s) 229, 657
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 1 cut(s) 363
XceI RCATGY 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.