Rw5G049580

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002477
Physical Location & Seq
Forward (+)
86073792 .. 86077747
3956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049580.1

Sequence Viewer

Length: 1080 bp
ATGGACCCAAACGCTGTGCAGTCTTCGAGGGGATACAGAGTTCAAGCTCCACTGGTTGACTCCGTATCATGTTATTGCAAAGTAGATGCAGGCTTAAAAACAGTTGCAGGGGCAAGAAAGTTTGTCCCAGGATCAAAACTTTGTATCCAGCCTGATATCAATCCAAACGCACACAGGGGAAAAAGTTCACGTAGGGAAAGGACCAGAATCCAGCCACCCCTTTTGCCTGGCCTACCTGATGATCTTGCAATTGCTTGTTTAATCAGAGTTCCTCGGGTGGAGCATAGAAAACTCCGTCTAGTTTGCAAAAGATGGTATCGCCTTCTGGCTGGAAACTTCTTTTATTCTCTTAGGAAAAGTCTTGGAATGGCAGAAGAGTGGGTTTATGTCATCAAAAGAGACCGCGATGGAAGAATCTCTTGGCATGCTTTTGATCCCACGTACCAGCTCTGGCAGCCACTTCCGCCTGTTCCTGGTGAATATTCTGCAGCGCTTGGTTTTGGCTGTGCTGTCCTTAGCGGTTGCCACCTGTACTTATTTGGAGGAAAAGATCCATTGAAGGGATCTATGAGACGGGTTATTTTTTACAGTGCTCGGACAAATAAATGGCATAGGGCACCAGATATGCTTCGAAAGCGGCATTTCTTTGGTGCTTGTGTTATTAATAACTGCCTTTATGTAGCTGGTGGAGAGTGTGAAGGAATTCAAAGGACTCTCCGTTCTGCTGAAATTTATGACCCTAACAAGAACCGGTGGAGCTTTATTTCAGATATGAGCACAGCTATGGTTCCCTTTATTGGGGTTGTTCACGATGGTATGTGGTTTCTAAAAGGACTTGGATCGCACCGTGAAGTAATGAGTGAAGCCTATTCTCCTGAAGCTAACACTTGGAACCCCATTAGTGATGGGATGGTTGCTGGTTGGCGCAACCCAAGTATTTCTTTAAATGGGCAACTCTATGCTCTGGATTGCCGTGATGGGTGCAAGGTTAGGGTTTATGATAGAGTGACAGATTCATGGAACAAATTTATTGATAGCAAGCTTCATCTGGGAAGCTTTGCATTATTCGTAATAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

359

Amino Acids

40.63

Weight (kDa)

9.56

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 76 - 112 3.1e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 140 - 298 3e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 151 - 263 1.4e-10 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 167 - 342 9.4e-18 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 187 - 301 1.5e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 213 - 259 1.2e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 616
AccII CGCG 1 cut(s) 405
AciI CCGC 4 cut(s) 403, 464, 519, 637
AclWI GGATC 5 cut(s) 139, 428, 545, 571, 847
AcsI RAATTY 3 cut(s) 702, 729, 1025
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 2 cut(s) 443, 533
AfeI AGCGCT 1 cut(s) 492
AfiI CCNNNNNNNGG 4 cut(s) 473, 560, 797, 798
AgeI ACCGGT 1 cut(s) 750
AgsI TTSAA 3 cut(s) 44, 559, 707
AjnI CCWGG 3 cut(s) 127, 226, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AloI GAACNNNNNNTCC 4 cut(s) 24, 56, 703, 735
AluBI AGCT 8 cut(s) 47, 448, 683, 759, 782, 881, 1042, 1056
AluI AGCT 8 cut(s) 47, 448, 683, 759, 782, 881, 1042, 1056
Alw21I GWGCWC 2 cut(s) 595, 779
Alw26I GTCTC 2 cut(s) 393, 565
AlwI GGATC 5 cut(s) 139, 428, 545, 571, 847
Ama87I CYCGRG 1 cut(s) 273
Aor51HI AGCGCT 1 cut(s) 492
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 454, 488
ApoI RAATTY 3 cut(s) 702, 729, 1025
AseI ATTAAT 1 cut(s) 663
AsiGI ACCGGT 1 cut(s) 750
Asp700I GAANNNNTTC 2 cut(s) 184, 702
AspLEI GCGC 2 cut(s) 493, 927
AspS9I GGNCC 2 cut(s) 4, 201
AsuHPI GGTGA 1 cut(s) 488
AsuII TTCGAA 1 cut(s) 631
AvaI CYCGRG 1 cut(s) 273
AvaII GGWCC 2 cut(s) 4, 201
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 595, 779
BbvI GCAGC 2 cut(s) 466, 500
BccI CCATC 6 cut(s) 306, 401, 806, 899, 904, 971
BceAI ACGGC 1 cut(s) 957
BciT130I CCWGG 3 cut(s) 129, 228, 474
BciVI GTATCC 2 cut(s) 26, 155
BcoDI GTCTC 2 cut(s) 393, 565
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 494
BfuI GTATCC 2 cut(s) 26, 155
BisI GCNGC 3 cut(s) 455, 489, 638
BlsI GCNGC 3 cut(s) 456, 490, 639
Bme1390I CCNGG 3 cut(s) 129, 228, 474
Bme18I GGWCC 2 cut(s) 4, 201
BmeT110I CYCGRG 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 4, 201
BmiI GGNNCC 4 cut(s) 6, 618, 789, 893
BmrFI CCNGG 3 cut(s) 129, 228, 474
BmsI GCATC 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 631
BsaAI YACGTR 2 cut(s) 191, 441
BsaBI GATNNNNATC 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 127, 272
BsaWI WCCGGW 1 cut(s) 750
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 560, 797, 798
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 3 cut(s) 129, 228, 474
BseDI CCNNGG 2 cut(s) 127, 272
BseGI GGATG 1 cut(s) 915
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 4 cut(s) 473, 560, 797, 798
BseNI ACTGG 1 cut(s) 57
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 2 cut(s) 466, 500
BsgI GTGCAG 1 cut(s) 38
Bsh1236I CGCG 1 cut(s) 405
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 616
BshTI ACCGGT 1 cut(s) 750
BsiHKAI GWGCWC 2 cut(s) 595, 779
BsiHKCI CYCGRG 1 cut(s) 273
BsiSI CCGG 1 cut(s) 751
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 4 cut(s) 473, 560, 797, 798
BsmAI GTCTC 2 cut(s) 393, 565
BsmBI CGTCTC 1 cut(s) 565
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 1 cut(s) 231
Bso31I GGTCTC 1 cut(s) 393
BsoBI CYCGRG 1 cut(s) 273
Bsp119I TTCGAA 1 cut(s) 631
Bsp1286I GDGCHC 3 cut(s) 595, 619, 779
Bsp143I GATC 6 cut(s) 131, 241, 433, 550, 563, 839
BspACI CCGC 4 cut(s) 403, 464, 519, 637
BspANI GGCC 1 cut(s) 231
BspFNI CGCG 1 cut(s) 405
BspLI GGNNCC 4 cut(s) 6, 618, 789, 893
BspMAI CTGCAG 1 cut(s) 490
BspPI GGATC 5 cut(s) 139, 428, 545, 571, 847
BspT104I TTCGAA 1 cut(s) 631
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 750
BsrI ACTGG 1 cut(s) 57
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 2 cut(s) 127, 272
BssMI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
Bst2UI CCWGG 3 cut(s) 129, 228, 474
Bst4CI ACNGT 3 cut(s) 103, 590, 848
Bst6I CTCTTC 1 cut(s) 369
BstBAI YACGTR 2 cut(s) 191, 441
BstBI TTCGAA 1 cut(s) 631
BstC8I GCNNGC 3 cut(s) 91, 426, 1040
BstDEI CTNAG 2 cut(s) 350, 515
BstF5I GGATG 1 cut(s) 915
BstFNI CGCG 1 cut(s) 405
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 2 cut(s) 493, 927
BstKTI GATC 6 cut(s) 134, 244, 436, 553, 566, 842
BstMAI GTCTC 2 cut(s) 393, 565
BstMBI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
BstMWI GCNNNNNNNGC 3 cut(s) 454, 463, 634
BstNI CCWGG 3 cut(s) 129, 228, 474
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 127, 226, 472
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 619
BstUI CGCG 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 466, 500
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 550, 563
BstYI RGATCY 2 cut(s) 550, 563
BsuI GTATCC 2 cut(s) 26, 155
BsuRI GGCC 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 420
BtsCI GGATG 1 cut(s) 915
BtsIMutI CAGTG 2 cut(s) 50, 595
Cac8I GCNNGC 3 cut(s) 91, 426, 1040
CfoI GCGC 2 cut(s) 493, 927
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 2 cut(s) 4, 201
Csp6I GTAC 2 cut(s) 442, 532
CspAI ACCGGT 1 cut(s) 750
CspCI CAANNNNNGTGG 2 cut(s) 204, 239
CviAII CATG 4 cut(s) 69, 425, 1017, 1077
CviQI GTAC 2 cut(s) 442, 532
DdeI CTNAG 2 cut(s) 350, 515
DpnI GATC 6 cut(s) 133, 243, 435, 552, 565, 841
DpnII GATC 6 cut(s) 131, 241, 433, 550, 563, 839
DraI TTTAAA 1 cut(s) 945
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EciI GGCGGA 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 4, 201
Eco47III AGCGCT 1 cut(s) 492
Eco57I CTGAAG 1 cut(s) 897
Eco88I CYCGRG 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 702
EcoRII CCWGG 3 cut(s) 127, 226, 472
EcoRV GATATC 1 cut(s) 157
Esp3I CGTCTC 1 cut(s) 565
FaeI CATG 4 cut(s) 72, 428, 1020, 1080
FalI AAGNNNNNCTT 4 cut(s) 403, 435, 925, 957
FaqI GGGAC 1 cut(s) 110
FatI CATG 4 cut(s) 68, 424, 1016, 1076
Fnu4HI GCNGC 3 cut(s) 455, 489, 638
FokI GGATG 1 cut(s) 922
Fsp4HI GCNGC 3 cut(s) 455, 489, 638
FspBI CTAG 1 cut(s) 299
GlaI GCGC 2 cut(s) 492, 926
GluI GCNGC 3 cut(s) 455, 489, 638
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 1 cut(s) 231
HapII CCGG 1 cut(s) 751
HhaI GCGC 2 cut(s) 493, 927
Hin1II CATG 4 cut(s) 72, 428, 1020, 1080
Hin6I GCGC 2 cut(s) 491, 925
HinP1I GCGC 2 cut(s) 491, 925
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 2 cut(s) 1040, 1054
HinfI GANTC 5 cut(s) 59, 207, 414, 712, 1013
HpaII CCGG 1 cut(s) 751
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 3 cut(s) 58, 188, 808
Hpy188I TCNGA 3 cut(s) 266, 597, 769
Hpy188III TCNNGA 3 cut(s) 809, 875, 965
Hpy8I GTNNAC 3 cut(s) 58, 188, 808
HpyAV CCTTC 3 cut(s) 332, 553, 692
HpyCH4III ACNGT 3 cut(s) 103, 590, 848
HpyCH4IV ACGT 2 cut(s) 190, 440
HpyCH4V TGCA 9 cut(s) 19, 78, 89, 107, 248, 306, 488, 984, 1061
HpyF10VI GCNNNNNNNGC 3 cut(s) 454, 463, 634
HpyF3I CTNAG 2 cut(s) 350, 515
HpySE526I ACGT 2 cut(s) 190, 440
Hsp92II CATG 4 cut(s) 72, 428, 1020, 1080
HspAI GCGC 2 cut(s) 491, 925
Kzo9I GATC 6 cut(s) 131, 241, 433, 550, 563, 839
LmnI GCTCC 3 cut(s) 52, 280, 756
Lsp1109I GCAGC 2 cut(s) 466, 500
LweI GCATC 1 cut(s) 76
MaeI CTAG 1 cut(s) 299
MaeII ACGT 2 cut(s) 190, 440
MaeIII GTNAC 1 cut(s) 1006
MalI GATC 6 cut(s) 133, 243, 435, 552, 565, 841
MboI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
MboII GAAGA 3 cut(s) 15, 386, 423
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 2 cut(s) 550, 563
MhlI GDGCHC 3 cut(s) 595, 619, 779
MluCI AATT 4 cut(s) 249, 702, 729, 1025
MlyI GAGTC 2 cut(s) 53, 706
MnlI CCTC 3 cut(s) 21, 282, 536
MroXI GAANNNNTTC 2 cut(s) 184, 702
MseI TTAA 4 cut(s) 95, 260, 663, 944
MslI CAYNNNNRTG 1 cut(s) 782
MspI CCGG 1 cut(s) 751
MspR9I CCNGG 3 cut(s) 129, 228, 474
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 3 cut(s) 129, 228, 474
MvnI CGCG 1 cut(s) 405
MwoI GCNNNNNNNGC 3 cut(s) 454, 463, 634
NdeII GATC 6 cut(s) 131, 241, 433, 550, 563, 839
NlaIII CATG 4 cut(s) 72, 428, 1020, 1080
NlaIV GGNNCC 4 cut(s) 6, 618, 789, 893
NmuCI GTSAC 1 cut(s) 1006
NspI RCATGY 1 cut(s) 428
NspV TTCGAA 1 cut(s) 631
PaeI GCATGC 1 cut(s) 428
PdmI GAANNNNTTC 2 cut(s) 184, 702
PfeI GAWTC 3 cut(s) 207, 414, 1013
PinAI ACCGGT 1 cut(s) 750
PkrI GCNGC 3 cut(s) 456, 490, 639
PleI GAGTC 2 cut(s) 53, 706
PpsI GAGTC 2 cut(s) 53, 706
Ppu21I YACGTR 2 cut(s) 191, 441
PshBI ATTAAT 1 cut(s) 663
Psp6I CCWGG 3 cut(s) 127, 226, 472
PspGI CCWGG 3 cut(s) 127, 226, 472
PspN4I GGNNCC 4 cut(s) 6, 618, 789, 893
PspPI GGNCC 2 cut(s) 4, 201
PstI CTGCAG 1 cut(s) 490
PsuI RGATCY 2 cut(s) 550, 563
RsaI GTAC 2 cut(s) 443, 533
RsaNI GTAC 2 cut(s) 442, 532
RseI CAYNNNNRTG 1 cut(s) 782
SaqAI TTAA 4 cut(s) 95, 260, 663, 944
SatI GCNGC 3 cut(s) 455, 489, 638
Sau3AI GATC 6 cut(s) 131, 241, 433, 550, 563, 839
Sau96I GGNCC 2 cut(s) 4, 201
SchI GAGTC 2 cut(s) 53, 706
ScrFI CCNGG 3 cut(s) 129, 228, 474
SduI GDGCHC 3 cut(s) 595, 619, 779
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 631
SinI GGWCC 2 cut(s) 4, 201
SmiMI CAYNNNNRTG 1 cut(s) 782
SphI GCATGC 1 cut(s) 428
Sse9I AATT 4 cut(s) 249, 702, 729, 1025
SsiI CCGC 4 cut(s) 403, 464, 519, 637
SspI AATATT 1 cut(s) 482
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 3 cut(s) 127, 226, 472
TaaI ACNGT 3 cut(s) 103, 590, 848
TaiI ACGT 2 cut(s) 193, 443
TaqI TCGA 2 cut(s) 26, 631
TasI AATT 4 cut(s) 249, 702, 729, 1025
TatI WGTACW 1 cut(s) 531
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 3 cut(s) 207, 414, 1013
Tru1I TTAA 4 cut(s) 95, 260, 663, 944
Tru9I TTAA 4 cut(s) 95, 260, 663, 944
TscAI CASTG 2 cut(s) 57, 595
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 2 cut(s) 454, 488
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 2 cut(s) 1005, 1034
TspGWI ACGGA 3 cut(s) 52, 284, 707
TspRI CASTG 2 cut(s) 57, 595
VpaK11BI GGWCC 2 cut(s) 4, 201
VspI ATTAAT 1 cut(s) 663
XapI RAATTY 3 cut(s) 702, 729, 1025
XceI RCATGY 1 cut(s) 428
XmnI GAANNNNTTC 2 cut(s) 184, 702
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.