Rroxscaffold_2G00152320

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002477
Physical Location & Seq
Forward (+)
89316663 .. 89321351
4689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152320.1

Sequence Viewer

Length: 1419 bp
ATGCACCACATATATAACACATTGAAAATTCCAAACCAGAGCTCATACTCTGCAAGAGTAGTCCAAGATAATAAAACCCCAACTGTGGAAAATAATGAAGTTGCTAATGTGTTGAAGGAAGAAAAGATGGACAGAAGAAGAATGATACAGCCTCCATTGGTGGATAGCACAGCATGTTTATGCAGAGTTGATGCTGGTCTTAAAACTGTTGCTGGAGCAAAAAAGTATGTCCCTGGGGCAAAGCTTTGTCTGCAACCAGACATTAGAGCATCCATTCATCCAACTAGGAGTAAGCCTGCACGTGGTGAGAGTAGCCGCAATCAATCCCCTCTTCTTCCTGGACTCCCCGATGATCTTGCTATTGCTTGCCTAATTCGGGTCCCAAGAATGGAGCATCGCAAGCTCCGCCTAGTTTGCAAAAGATGGTACCGTCTTCTGGCTGGCAACTTCTTTTACTCCCTCCGTAAGAGCATTGGCATTGCTGAAGAATGGATATACTTGATTAAAAGAGATCGAGATGGGAAAATATCTTGGCATGCCTTTGACCCTGTATACCAGCTATGGCAGTCCCTTCCTCCTGTCCCTAAGGAATATTCTGAAGCTCTTGGATTTGGATGTGCTGTACTCGGTGGCTGTCACCTCTATCTGTTTGGAGGAAAGGACCCTCTAAAGGGATCAATGAGACGAGTCATTTTTTACAGTGCCAGGACCAATAAATGGCATCGTGCCCCAGATATGCTTCGCAGACGACATTTTTTTGGCTCGTGTGTTATAAATAACTGCCTCTATGTTGCTGGTGGAGAGAATGAGGGGGTGCATAGGTCCTTGAGATCAGCAGAAGTCTATGATCCCAACAAGAATAGATGGTCCTTCATTTCAGATATGAGCACTGCAATGGTGCCTTTCATCGGGGTTGTCTACGAAGGCAAATGGTTCTTGAAGGGGCTTGGGTCTCATCGACAAGTCCTGAGTGAGGTGTATCAACCAGAAAATGACAGCTGGTACTCTGTGTATGACGGAATGGTTGCAGGCTGGAGGAACCCCAGTGCTTCTTTAAATGGGCATCTCTATGCTTTGGAGTGCAAGGATGGCTGCAAACTCAGAGTTTACGATGAAGCAACTGATTCTTGGAGCAAGCATATCGACAGTAAGATACATTTGGGTAACTCTAGGGCCTTGGAGGCAGCTTCCCTGGTTCCCCTTAACGGTAAACTTTGCATCATCCGAAATAATATGAGTATTTCGCTTGTTGATGTTTCGAAATCAAATGATGCTGGTGGAGCAAGTGCTGAACATCTATGGGAAACTCTAGCAGGCAAAGGGCAGTTCAGGACAATGGTCACAAATCTGTGGTCAAGCCTTGCTGGCAGAAACCGCCTCAAAAGTCACATAGTTCACTGCCAGGTTCTTCAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

472

Amino Acids

53.25

Weight (kDa)

9.49

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 113 - 149 2.1e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 177 - 336 8e-11 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 186 - 299 7.6e-10 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 191 - 379 1.4e-19 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 224 - 335 2.8e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 249 - 296 4e-15 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 2 cut(s) 426, 898
AccB7I CCANNNNNTGG 1 cut(s) 717
AccI GTMKAC 2 cut(s) 552, 918
AciI CCGC 3 cut(s) 316, 406, 1375
AclWI GGATC 2 cut(s) 682, 842
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 2 cut(s) 504, 618
AcvI CACGTG 1 cut(s) 302
AdeI CACNNNGTG 1 cut(s) 305
AfaI GTAC 3 cut(s) 428, 624, 1004
AgsI TTSAA 4 cut(s) 25, 115, 940, 1412
AjnI CCWGG 5 cut(s) 232, 337, 704, 1191, 1401
AluBI AGCT 8 cut(s) 42, 244, 403, 559, 602, 999, 1187, 1415
AluI AGCT 8 cut(s) 42, 244, 403, 559, 602, 999, 1187, 1415
Alw21I GWGCWC 2 cut(s) 44, 890
Alw26I GTCTC 2 cut(s) 676, 957
AlwI GGATC 2 cut(s) 682, 842
AoxI GGCC 1 cut(s) 1173
ApeKI GCWGC 2 cut(s) 1092, 1184
ApoI RAATTY 1 cut(s) 27
ArsI GACNNNNNNTTYG 2 cut(s) 1337, 1369
Asp718I GGTACC 1 cut(s) 426
AspS9I GGNCC 6 cut(s) 379, 661, 708, 822, 867, 1173
AsuHPI GGTGA 2 cut(s) 317, 629
AsuII TTCGAA 1 cut(s) 1259
AvaII GGWCC 5 cut(s) 379, 661, 708, 822, 867
AxyI CCTNAGG 1 cut(s) 585
BaeGI GKGCMC 1 cut(s) 730
BanI GGYRCC 2 cut(s) 426, 898
BanII GRGCYC 1 cut(s) 44
BauI CACGAG 1 cut(s) 763
BbrPI CACGTG 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 425
Bbv12I GWGCWC 2 cut(s) 44, 890
BbvI GCAGC 2 cut(s) 1079, 1196
BccI CCATC 5 cut(s) 121, 417, 512, 858, 1082
BcgI CGANNNNNNTGC 4 cut(s) 338, 372, 1123, 1157
BciT130I CCWGG 5 cut(s) 234, 339, 706, 1193, 1403
BcoDI GTCTC 2 cut(s) 676, 957
BfaI CTAG 4 cut(s) 285, 410, 1170, 1310
BglI GCCNNNNNGGC 2 cut(s) 1181, 1365
BisI GCNGC 3 cut(s) 316, 1093, 1185
BlsI GCNGC 3 cut(s) 317, 1094, 1186
Bme1390I CCNGG 5 cut(s) 234, 339, 706, 1193, 1403
Bme18I GGWCC 5 cut(s) 379, 661, 708, 822, 867
BmgT120I GGNCC 6 cut(s) 379, 661, 708, 822, 867, 1173
BmiI GGNNCC 7 cut(s) 380, 381, 428, 663, 900, 1040, 1197
BmrFI CCNGG 5 cut(s) 234, 339, 706, 1193, 1403
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 7 cut(s) 181, 278, 403, 730, 1072, 1227, 1261
BmuI ACTGGG 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 1337
BpiI GAAGAC 1 cut(s) 425
BpmI CTGGAG 2 cut(s) 234, 1054
Bpu14I TTCGAA 1 cut(s) 1259
BpuEI CTTGAG 1 cut(s) 847
BsaAI YACGTR 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 4 cut(s) 232, 233, 1176, 1191
Bse1I ACTGG 1 cut(s) 1044
Bse21I CCTNAGG 1 cut(s) 585
Bse3DI GCAATG 2 cut(s) 477, 900
BseBI CCWGG 5 cut(s) 234, 339, 706, 1193, 1403
BseDI CCNNGG 4 cut(s) 232, 233, 1176, 1191
BseGI GGATG 5 cut(s) 269, 277, 620, 1093, 1221
BseMI GCAATG 2 cut(s) 477, 900
BseMII CTCAG 2 cut(s) 959, 1114
BseNI ACTGG 1 cut(s) 1044
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 2 cut(s) 1079, 1196
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 1 cut(s) 1175
BshNI GGYRCC 2 cut(s) 426, 898
BsiHKAI GWGCWC 2 cut(s) 44, 890
BslFI GGGAC 4 cut(s) 215, 365, 553, 566
BsmAI GTCTC 2 cut(s) 676, 957
BsmBI CGTCTC 1 cut(s) 676
BsmFI GGGAC 4 cut(s) 215, 365, 553, 566
BsnI GGCC 1 cut(s) 1175
Bso31I GGTCTC 1 cut(s) 957
Bsp119I TTCGAA 1 cut(s) 1259
Bsp1286I GDGCHC 3 cut(s) 44, 730, 890
Bsp143I GATC 5 cut(s) 352, 511, 674, 830, 847
BspACI CCGC 3 cut(s) 316, 406, 1375
BspANI GGCC 1 cut(s) 1175
BspCNI CTCAG 2 cut(s) 960, 1113
BspLI GGNNCC 7 cut(s) 380, 381, 428, 663, 900, 1040, 1197
BspPI GGATC 2 cut(s) 682, 842
BspT104I TTCGAA 1 cut(s) 1259
BspT107I GGYRCC 2 cut(s) 426, 898
BspTNI GGTCTC 1 cut(s) 957
BsrDI GCAATG 2 cut(s) 477, 900
BsrI ACTGG 1 cut(s) 1044
BssECI CCNNGG 4 cut(s) 232, 233, 1176, 1191
BssMI GATC 5 cut(s) 352, 511, 674, 830, 847
BssNAI GTATAC 1 cut(s) 553
BssSI CACGAG 1 cut(s) 763
BssT1I CCWWGG 1 cut(s) 1176
Bst1107I GTATAC 1 cut(s) 553
Bst2BI CACGAG 1 cut(s) 763
Bst2UI CCWGG 5 cut(s) 234, 339, 706, 1193, 1403
Bst4CI ACNGT 6 cut(s) 85, 208, 431, 701, 1148, 1208
Bst6I CTCTTC 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 1259
BstC8I GCNNGC 9 cut(s) 297, 367, 401, 442, 537, 1030, 1136, 1315, 1366
BstDEI CTNAG 4 cut(s) 585, 968, 1100, 1416
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 5 cut(s) 269, 277, 620, 1093, 1221
BstKTI GATC 5 cut(s) 355, 514, 677, 833, 850
BstMAI GTCTC 2 cut(s) 676, 957
BstMBI GATC 5 cut(s) 352, 511, 674, 830, 847
BstMWI GCNNNNNNNGC 9 cut(s) 250, 400, 405, 414, 1089, 1181, 1280, 1365, 1374
BstNI CCWGG 5 cut(s) 234, 339, 706, 1193, 1403
BstNSI RCATGY 2 cut(s) 177, 539
BstPAI GACNNNNGTC 1 cut(s) 1337
BstSCI CCNGG 5 cut(s) 232, 337, 704, 1191, 1401
BstSLI GKGCMC 1 cut(s) 730
BstV1I GCAGC 2 cut(s) 1079, 1196
BstV2I GAAGAC 1 cut(s) 425
BstZ17I GTATAC 1 cut(s) 553
Bsu36I CCTNAGG 1 cut(s) 585
BsuRI GGCC 1 cut(s) 1175
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 5 cut(s) 269, 277, 620, 1093, 1221
BtsI GCAGTG 2 cut(s) 888, 1396
BtsIMutI CAGTG 4 cut(s) 706, 888, 1051, 1396
Cac8I GCNNGC 9 cut(s) 297, 367, 401, 442, 537, 1030, 1136, 1315, 1366
Cfr13I GGNCC 6 cut(s) 379, 661, 708, 822, 867, 1173
Csp6I GTAC 3 cut(s) 427, 623, 1003
CviAII CATG 2 cut(s) 174, 536
CviQI GTAC 3 cut(s) 427, 623, 1003
DdeI CTNAG 4 cut(s) 585, 968, 1100, 1416
DpnI GATC 5 cut(s) 354, 513, 676, 832, 849
DpnII GATC 5 cut(s) 352, 511, 674, 830, 847
DraI TTTAAA 1 cut(s) 1056
DraIII CACNNNGTG 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 395
Ecl136II GAGCTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 1176
Eco24I GRGCYC 1 cut(s) 44
Eco31I GGTCTC 1 cut(s) 957
Eco47I GGWCC 5 cut(s) 379, 661, 708, 822, 867
Eco53kI GAGCTC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 504, 618
Eco72I CACGTG 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 42
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoO109I RGGNCCY 4 cut(s) 379, 661, 822, 1173
EcoRII CCWGG 5 cut(s) 232, 337, 704, 1191, 1401
EcoT14I CCWWGG 1 cut(s) 1176
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 1176
Esp3I CGTCTC 1 cut(s) 676
FaeI CATG 2 cut(s) 177, 539
FaqI GGGAC 4 cut(s) 215, 365, 553, 566
FatI CATG 2 cut(s) 173, 535
FblI GTMKAC 2 cut(s) 552, 918
Fnu4HI GCNGC 3 cut(s) 316, 1093, 1185
FokI GGATG 5 cut(s) 256, 264, 627, 1100, 1208
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 316, 1093, 1185
FspBI CTAG 4 cut(s) 285, 410, 1170, 1310
GluI GCNGC 3 cut(s) 316, 1093, 1185
GsuI CTGGAG 2 cut(s) 234, 1054
HaeIII GGCC 1 cut(s) 1175
Hin1II CATG 2 cut(s) 177, 539
HindIII AAGCTT 2 cut(s) 242, 1413
HinfI GANTC 3 cut(s) 342, 687, 1124
HphI GGTGA 2 cut(s) 317, 629
Hpy166II GTNNAC 5 cut(s) 553, 919, 1108, 1211, 1396
Hpy188I TCNGA 4 cut(s) 598, 880, 1103, 1226
Hpy188III TCNNGA 4 cut(s) 515, 937, 967, 1330
Hpy8I GTNNAC 5 cut(s) 553, 919, 1108, 1211, 1396
HpyAV CCTTC 5 cut(s) 109, 581, 880, 917, 934
HpyCH4III ACNGT 6 cut(s) 85, 208, 431, 701, 1148, 1208
HpyCH4IV ACGT 1 cut(s) 301
HpyF10VI GCNNNNNNNGC 9 cut(s) 250, 400, 405, 414, 1089, 1181, 1280, 1365, 1374
HpyF3I CTNAG 4 cut(s) 585, 968, 1100, 1416
HpySE526I ACGT 1 cut(s) 301
Hsp92II CATG 2 cut(s) 177, 539
KflI GGGWCCC 1 cut(s) 379
KpnI GGTACC 1 cut(s) 430
Kzo9I GATC 5 cut(s) 352, 511, 674, 830, 847
LmnI GCTCC 5 cut(s) 215, 391, 408, 1131, 1280
Lsp1109I GCAGC 2 cut(s) 1079, 1196
LweI GCATC 7 cut(s) 181, 278, 403, 730, 1072, 1227, 1261
MaeI CTAG 4 cut(s) 285, 410, 1170, 1310
MaeII ACGT 1 cut(s) 301
MaeIII GTNAC 4 cut(s) 635, 1163, 1339, 1385
MalI GATC 5 cut(s) 354, 513, 676, 832, 849
MboI GATC 5 cut(s) 352, 511, 674, 830, 847
MboII GAAGA 8 cut(s) 131, 147, 150, 323, 326, 425, 497, 1400
MhlI GDGCHC 3 cut(s) 44, 730, 890
MluCI AATT 2 cut(s) 27, 372
MlyI GAGTC 2 cut(s) 336, 696
MmeI TCCRAC 1 cut(s) 305
MseI TTAA 4 cut(s) 201, 504, 1055, 1203
MslI CAYNNNNRTG 3 cut(s) 178, 893, 1068
MspA1I CMGCKG 1 cut(s) 999
MspR9I CCNGG 5 cut(s) 234, 339, 706, 1193, 1403
MvaI CCWGG 5 cut(s) 234, 339, 706, 1193, 1403
MwoI GCNNNNNNNGC 9 cut(s) 250, 400, 405, 414, 1089, 1181, 1280, 1365, 1374
NdeII GATC 5 cut(s) 352, 511, 674, 830, 847
NlaIII CATG 2 cut(s) 177, 539
NlaIV GGNNCC 7 cut(s) 380, 381, 428, 663, 900, 1040, 1197
NmuCI GTSAC 3 cut(s) 635, 1339, 1385
NspI RCATGY 2 cut(s) 177, 539
NspV TTCGAA 1 cut(s) 1259
PaeI GCATGC 1 cut(s) 539
PasI CCCWGGG 1 cut(s) 233
PfeI GAWTC 1 cut(s) 1124
PflMI CCANNNNNTGG 1 cut(s) 717
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 3 cut(s) 317, 1094, 1186
PleI GAGTC 2 cut(s) 336, 695
PmaCI CACGTG 1 cut(s) 302
PmlI CACGTG 1 cut(s) 302
PpsI GAGTC 2 cut(s) 336, 695
Ppu21I YACGTR 1 cut(s) 302
PpuMI RGGWCCY 3 cut(s) 379, 661, 822
PshAI GACNNNNGTC 1 cut(s) 1337
PsiI TTATAA 1 cut(s) 773
Psp124BI GAGCTC 1 cut(s) 44
Psp5II RGGWCCY 3 cut(s) 379, 661, 822
Psp6I CCWGG 5 cut(s) 232, 337, 704, 1191, 1401
PspCI CACGTG 1 cut(s) 302
PspGI CCWGG 5 cut(s) 232, 337, 704, 1191, 1401
PspN4I GGNNCC 7 cut(s) 380, 381, 428, 663, 900, 1040, 1197
PspPI GGNCC 6 cut(s) 379, 661, 708, 822, 867, 1173
PspPPI RGGWCCY 3 cut(s) 379, 661, 822
PvuII CAGCTG 1 cut(s) 999
RsaI GTAC 3 cut(s) 428, 624, 1004
RsaNI GTAC 3 cut(s) 427, 623, 1003
RseI CAYNNNNRTG 3 cut(s) 178, 893, 1068
SacI GAGCTC 1 cut(s) 44
SaqAI TTAA 4 cut(s) 201, 504, 1055, 1203
SatI GCNGC 3 cut(s) 316, 1093, 1185
Sau3AI GATC 5 cut(s) 352, 511, 674, 830, 847
Sau96I GGNCC 6 cut(s) 379, 661, 708, 822, 867, 1173
SchI GAGTC 2 cut(s) 336, 696
ScrFI CCNGG 5 cut(s) 234, 339, 706, 1193, 1403
SduI GDGCHC 3 cut(s) 44, 730, 890
SfaNI GCATC 7 cut(s) 181, 278, 403, 730, 1072, 1227, 1261
SfuI TTCGAA 1 cut(s) 1259
SinI GGWCC 5 cut(s) 379, 661, 708, 822, 867
SmiMI CAYNNNNRTG 3 cut(s) 178, 893, 1068
SmlI CTYRAG 1 cut(s) 826
SmoI CTYRAG 1 cut(s) 826
SphI GCATGC 1 cut(s) 539
Sse9I AATT 2 cut(s) 27, 372
SsiI CCGC 3 cut(s) 316, 406, 1375
SspI AATATT 1 cut(s) 593
SspMI CTAG 4 cut(s) 285, 410, 1170, 1310
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 5 cut(s) 232, 337, 704, 1191, 1401
StyI CCWWGG 1 cut(s) 1176
TaaI ACNGT 6 cut(s) 85, 208, 431, 701, 1148, 1208
TaiI ACGT 1 cut(s) 304
TaqI TCGA 4 cut(s) 514, 958, 1143, 1259
TasI AATT 2 cut(s) 27, 372
TatI WGTACW 1 cut(s) 622
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 1124
Tru1I TTAA 4 cut(s) 201, 504, 1055, 1203
Tru9I TTAA 4 cut(s) 201, 504, 1055, 1203
TscAI CASTG 4 cut(s) 706, 895, 1051, 1403
TseFI GTSAC 3 cut(s) 635, 1339, 1385
TseI GCWGC 2 cut(s) 1092, 1184
Tsp45I GTSAC 3 cut(s) 635, 1339, 1385
TspDTI ATGAA 5 cut(s) 111, 266, 862, 895, 1128
TspGWI ACGGA 2 cut(s) 452, 1032
TspRI CASTG 4 cut(s) 706, 895, 1051, 1403
Van91I CCANNNNNTGG 1 cut(s) 717
VpaK11BI GGWCC 5 cut(s) 379, 661, 708, 822, 867
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 1 cut(s) 27
XceI RCATGY 2 cut(s) 177, 539
XmiI GTMKAC 2 cut(s) 552, 918
XspI CTAG 4 cut(s) 285, 410, 1170, 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.