MD03G1007500.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002477
Physical Location & Seq
Forward (+)
599108 .. 602080
2973 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1007500.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGGACCAGAACACTGAACACTCTTCCAATGCTCAGAGGGGCTTCAGAGTTCAAGCTCCGCTGGTTGACTCTGTATCATGCTATTGTAAAGTAGATGCAGGCTTCAAAACTGTTGCAGGGGCAAGAAAGTTTGTCCCAGGATCAAAGATTTGCATCCAGCCTGATATCAATCCTAATGCACACAGGGGAAAAAATTTACGCCGGGAAAGGACCAGAATCCAGCCACCCCTCCTGCCTGGTCTACCTGATGATCTTGCAATTGCTTGTTTAATCAGAGTCCCCCGTGTTGAGCATAGGAAACTCCGTATAGTTTGCAAAAGATGGTATCACCTTCTAGCGGGAAACTTCTTTTATTCGCTTAGGAAAAGTCTTGGAATGGCGGAAGAATGGGTTTATGTTATCAAAAGAGACCGTGATGGAAGGATCTCTTGGCATGCTTTTGATCCTACATACCAGCTCTGGCAGCCACTTCCACCCGTTCCTGGTGAGTATTCTGCCGCGCTTGGTTTTGGTTGTGCTGTCCTTAGCGGTTGTCACCTGTACTTGTTTGGAGGAAAACATCCATTAAGGGGATCTATGAGACGGGTCATTTTTTACAGTGCTCGGACAAATAAATGGCACAGGGCACCAGATATGCTTCGGAAACGCCATTTCTTTGGCTCTTGTGTTATTAATAATTGTCTTTATGTAGCTGGTGGGGAGTGTGAAGGAATTCAAAGGACTCTTCGTTCTGCTGAAATTTATGATCCAAACAAAAATCGGTGGACCTTTATTTCCGATATGAGCACAGCTATGGTGCCATTTATTGGGGTGGTTCATGACGGAATGTGGTTCCTAAAAGGACTTGGGTCACACCGTGAGGTAATGAGTGAAGCCTATACTCCTGAAGCCAATACCTGGACCCCTATTAGTGACGGGATGGTTGCTGGGTGGCGCAACCCGAGTATTTCTTTAAATGGGCAGCTCTATGCTCTGGACTGCCGTGATGGGTGCAAGCTTAGGGTTTATGATAGAGTATCAGATTCATGGAACAAATTTATAGATAGCAAGGTTCATCTAGGGAGTTCTTGTGCTTTGGAGGCTGCTGCGCTAGTTCCCCTTAACGGGAAGCTTTGCATTATTCGTAATAACATGAGCATCAGTTTAGTTGATGTTTCAAGTCCGGATAAACATGTCGAAAGCAACCCTCACCTTTGGGAAAATATCGCCGGCAAAGGTCATTTCAGAACTCTAGTCTCAAATATATGGTCAAGTATAGCAGGCCGAACTGGTTTGAAGAGTCACATTGTACACTGTCAAGTGCTCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

438

Amino Acids

49.2

Weight (kDa)

9.47

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 143 - 302 2.8e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 170 - 358 2.9e-17 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 190 - 307 1.1e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 216 - 262 3.2e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 625, 796
AccB7I CCANNNNNTGG 2 cut(s) 806, 897
AccI GTMKAC 1 cut(s) 241
AccII CGCG 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 1162
AciI CCGC 5 cut(s) 59, 338, 380, 498, 528
AclWI GGATC 5 cut(s) 148, 431, 437, 580, 740
AcsI RAATTY 4 cut(s) 193, 711, 738, 1034
AcuI CTGAAG 2 cut(s) 28, 906
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 2 cut(s) 542, 1290
AfiI CCNNNNNNNGG 7 cut(s) 337, 482, 569, 806, 897, 1103, 1104
AflIII ACRYGT 1 cut(s) 1171
AgsI TTSAA 5 cut(s) 53, 106, 716, 1158, 1276
AjnI CCWGG 4 cut(s) 136, 235, 481, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 412, 444
AloI GAACNNNNNNTCC 2 cut(s) 712, 744
AluBI AGCT 7 cut(s) 56, 457, 692, 791, 964, 997, 1111
AluI AGCT 7 cut(s) 56, 457, 692, 791, 964, 997, 1111
Alw21I GWGCWC 3 cut(s) 604, 788, 1305
Alw26I GTCTC 3 cut(s) 402, 574, 1240
AlwI GGATC 5 cut(s) 148, 431, 437, 580, 740
Ama87I CYCGRG 1 cut(s) 940
Aor13HI TCCGGA 1 cut(s) 1162
AoxI GGCC 1 cut(s) 1261
ApeKI GCWGC 4 cut(s) 463, 961, 1082, 1085
ApoI RAATTY 4 cut(s) 193, 711, 738, 1034
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 3 cut(s) 502, 936, 1090
AspS9I GGNCC 4 cut(s) 4, 210, 765, 900
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 4 cut(s) 320, 497, 527, 1181
AvaI CYCGRG 1 cut(s) 940
AvaII GGWCC 4 cut(s) 4, 210, 765, 900
BaeGI GKGCMC 1 cut(s) 628
BanI GGYRCC 2 cut(s) 625, 796
Bbv12I GWGCWC 3 cut(s) 604, 788, 1305
BbvI GCAGC 4 cut(s) 475, 973, 1069, 1072
BccI CCATC 4 cut(s) 315, 410, 913, 980
BceAI ACGGC 1 cut(s) 966
BciT130I CCWGG 4 cut(s) 138, 237, 483, 898
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 402, 574, 1240
BfaI CTAG 4 cut(s) 335, 1058, 1091, 1232
BisI GCNGC 5 cut(s) 464, 498, 962, 1083, 1086
BlsI GCNGC 5 cut(s) 465, 499, 963, 1084, 1087
Bme1390I CCNGG 5 cut(s) 138, 203, 237, 483, 898
Bme18I GGWCC 4 cut(s) 4, 210, 765, 900
BmeT110I CYCGRG 1 cut(s) 940
BmgT120I GGNCC 4 cut(s) 4, 210, 765, 900
BmiI GGNNCC 4 cut(s) 627, 798, 833, 902
BmrFI CCNGG 5 cut(s) 138, 203, 237, 483, 898
BmsI GCATC 3 cut(s) 85, 162, 1146
BoxI GACNNNNGTC 1 cut(s) 847
Bpu10I CCTNAGC 3 cut(s) 359, 524, 998
BpuMI CCSGG 1 cut(s) 203
BsaBI GATNNNNATC 2 cut(s) 152, 168
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 2 cut(s) 1054, 1084
Bsc4I CCNNNNNNNGG 7 cut(s) 337, 482, 569, 806, 897, 1103, 1104
Bse118I RCCGGY 1 cut(s) 1208
Bse1I ACTGG 1 cut(s) 1273
Bse8I GATNNNNATC 2 cut(s) 152, 168
BseAI TCCGGA 1 cut(s) 1162
BseBI CCWGG 4 cut(s) 138, 237, 483, 898
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 153, 559, 924
BseJI GATNNNNATC 2 cut(s) 152, 168
BseLI CCNNNNNNNGG 7 cut(s) 337, 482, 569, 806, 897, 1103, 1104
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 1273
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 475, 973, 1069, 1072
BseYI CCCAGC 1 cut(s) 926
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 1 cut(s) 1263
BshNI GGYRCC 2 cut(s) 625, 796
BsiHKAI GWGCWC 3 cut(s) 604, 788, 1305
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 3 cut(s) 202, 1163, 1209
BslFI GGGAC 2 cut(s) 119, 263
BslI CCNNNNNNNGG 7 cut(s) 337, 482, 569, 806, 897, 1103, 1104
BsmAI GTCTC 3 cut(s) 402, 574, 1240
BsmBI CGTCTC 1 cut(s) 574
BsmFI GGGAC 2 cut(s) 119, 263
BsnI GGCC 1 cut(s) 1263
Bso31I GGTCTC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 4 cut(s) 604, 628, 788, 1305
Bsp13I TCCGGA 1 cut(s) 1162
Bsp1407I TGTACA 1 cut(s) 1288
Bsp143I GATC 6 cut(s) 140, 250, 423, 442, 572, 745
BspACI CCGC 5 cut(s) 59, 338, 380, 498, 528
BspANI GGCC 1 cut(s) 1263
BspCNI CTCAG 1 cut(s) 46
BspEI TCCGGA 1 cut(s) 1162
BspFNI CGCG 1 cut(s) 500
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 4 cut(s) 627, 798, 833, 902
BspPI GGATC 5 cut(s) 148, 431, 437, 580, 740
BspT107I GGYRCC 2 cut(s) 625, 796
BspTNI GGTCTC 1 cut(s) 402
BsrFI RCCGGY 1 cut(s) 1208
BsrGI TGTACA 1 cut(s) 1288
BsrI ACTGG 1 cut(s) 1273
BssAI RCCGGY 1 cut(s) 1208
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 6 cut(s) 140, 250, 423, 442, 572, 745
Bst2UI CCWGG 4 cut(s) 138, 237, 483, 898
Bst4CI ACNGT 5 cut(s) 112, 413, 599, 857, 1295
Bst6I CTCTTC 3 cut(s) 28, 729, 1271
BstAUI TGTACA 1 cut(s) 1288
BstC8I GCNNGC 5 cut(s) 100, 435, 995, 1210, 1261
BstDEI CTNAG 4 cut(s) 33, 359, 524, 998
BstF5I GGATG 3 cut(s) 153, 559, 924
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 3 cut(s) 502, 936, 1090
BstKTI GATC 6 cut(s) 143, 253, 426, 445, 575, 748
BstMAI GTCTC 3 cut(s) 402, 574, 1240
BstMBI GATC 6 cut(s) 140, 250, 423, 442, 572, 745
BstMWI GCNNNNNNNGC 2 cut(s) 463, 1079
BstNI CCWGG 4 cut(s) 138, 237, 483, 898
BstNSI RCATGY 2 cut(s) 437, 1175
BstPAI GACNNNNGTC 1 cut(s) 847
BstSCI CCNGG 5 cut(s) 136, 201, 235, 481, 896
BstSLI GKGCMC 1 cut(s) 628
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 4 cut(s) 475, 973, 1069, 1072
BstX2I RGATCY 2 cut(s) 423, 572
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 2 cut(s) 423, 572
BsuRI GGCC 1 cut(s) 1263
BtsCI GGATG 3 cut(s) 153, 559, 924
BtsIMutI CAGTG 3 cut(s) 12, 604, 1291
Cac8I GCNNGC 5 cut(s) 100, 435, 995, 1210, 1261
CciI TCATGA 1 cut(s) 817
CfoI GCGC 3 cut(s) 502, 936, 1090
Cfr10I RCCGGY 1 cut(s) 1208
Cfr13I GGNCC 4 cut(s) 4, 210, 765, 900
Csp6I GTAC 2 cut(s) 541, 1289
CviAII CATG 7 cut(s) 78, 434, 818, 1026, 1132, 1172, 1311
CviQI GTAC 2 cut(s) 541, 1289
DdeI CTNAG 4 cut(s) 33, 359, 524, 998
DpnI GATC 6 cut(s) 142, 252, 425, 444, 574, 747
DpnII GATC 6 cut(s) 140, 250, 423, 442, 572, 745
DraI TTTAAA 1 cut(s) 954
DraIII CACNNNGTG 1 cut(s) 857
Eam1104I CTCTTC 3 cut(s) 28, 729, 1271
EarI CTCTTC 3 cut(s) 28, 729, 1271
EciI GGCGGA 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 402
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 4 cut(s) 4, 210, 765, 900
Eco57I CTGAAG 2 cut(s) 28, 906
Eco88I CYCGRG 1 cut(s) 940
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 4 cut(s) 136, 235, 481, 896
EcoRV GATATC 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 574
FaeI CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FaqI GGGAC 2 cut(s) 119, 263
FatI CATG 7 cut(s) 77, 433, 817, 1025, 1131, 1171, 1310
FauI CCCGC 1 cut(s) 331
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 5 cut(s) 464, 498, 962, 1083, 1086
FokI GGATG 3 cut(s) 140, 546, 931
Fsp4HI GCNGC 5 cut(s) 464, 498, 962, 1083, 1086
FspBI CTAG 4 cut(s) 335, 1058, 1091, 1232
GlaI GCGC 3 cut(s) 501, 935, 1089
GluI GCNGC 5 cut(s) 464, 498, 962, 1083, 1086
GsaI CCCAGC 1 cut(s) 930
HaeIII GGCC 1 cut(s) 1263
HapII CCGG 3 cut(s) 202, 1163, 1209
HhaI GCGC 3 cut(s) 502, 936, 1090
Hin1II CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
Hin6I GCGC 3 cut(s) 500, 934, 1088
HinP1I GCGC 3 cut(s) 500, 934, 1088
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 2 cut(s) 995, 1109
HinfI GANTC 6 cut(s) 68, 216, 276, 721, 1022, 1279
HpaII CCGG 3 cut(s) 202, 1163, 1209
HphI GGTGA 4 cut(s) 320, 497, 527, 1181
Hpy166II GTNNAC 4 cut(s) 67, 242, 765, 1291
Hpy188I TCNGA 8 cut(s) 36, 47, 275, 606, 642, 778, 1021, 1226
Hpy188III TCNNGA 4 cut(s) 818, 884, 974, 1163
Hpy8I GTNNAC 4 cut(s) 67, 242, 765, 1291
HpyAV CCTTC 3 cut(s) 341, 414, 701
HpyCH4III ACNGT 5 cut(s) 112, 413, 599, 857, 1295
HpyCH4V TGCA 8 cut(s) 98, 116, 153, 179, 257, 315, 993, 1116
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 1079
HpyF3I CTNAG 4 cut(s) 33, 359, 524, 998
Hsp92II CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
HspAI GCGC 3 cut(s) 500, 934, 1088
Kpn2I TCCGGA 1 cut(s) 1162
KroI GCCGGC 1 cut(s) 1208
KroNI GCCGGC 1 cut(s) 1210
Kzo9I GATC 6 cut(s) 140, 250, 423, 442, 572, 745
LmnI GCTCC 2 cut(s) 61, 1308
Lsp1109I GCAGC 4 cut(s) 475, 973, 1069, 1072
LweI GCATC 3 cut(s) 85, 162, 1146
MaeI CTAG 4 cut(s) 335, 1058, 1091, 1232
MaeIII GTNAC 4 cut(s) 533, 849, 911, 1280
MalI GATC 6 cut(s) 142, 252, 425, 444, 574, 747
MboI GATC 6 cut(s) 140, 250, 423, 442, 572, 745
MboII GAAGA 4 cut(s) 15, 395, 716, 1288
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 2 cut(s) 423, 572
MhlI GDGCHC 4 cut(s) 604, 628, 788, 1305
MluCI AATT 6 cut(s) 193, 258, 676, 711, 738, 1034
MlyI GAGTC 4 cut(s) 62, 285, 715, 1288
MnlI CCTC 6 cut(s) 30, 239, 545, 853, 1072, 1197
MroI TCCGGA 1 cut(s) 1162
MroNI GCCGGC 1 cut(s) 1208
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 5 cut(s) 269, 566, 672, 953, 1101
MslI CAYNNNNRTG 1 cut(s) 791
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 3 cut(s) 202, 1163, 1209
MspR9I CCNGG 5 cut(s) 138, 203, 237, 483, 898
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 4 cut(s) 138, 237, 483, 898
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 2 cut(s) 463, 1079
NaeI GCCGGC 1 cut(s) 1210
NciI CCSGG 1 cut(s) 203
NdeII GATC 6 cut(s) 140, 250, 423, 442, 572, 745
NgoMIV GCCGGC 1 cut(s) 1208
NlaIII CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
NlaIV GGNNCC 4 cut(s) 627, 798, 833, 902
NmuCI GTSAC 4 cut(s) 533, 849, 911, 1280
NspI RCATGY 2 cut(s) 437, 1175
PaeI GCATGC 1 cut(s) 437
PagI TCATGA 1 cut(s) 817
PciI ACATGT 1 cut(s) 1171
PdiI GCCGGC 1 cut(s) 1210
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 2 cut(s) 216, 1022
PflMI CCANNNNNTGG 2 cut(s) 806, 897
PkrI GCNGC 5 cut(s) 465, 499, 963, 1084, 1087
PleI GAGTC 4 cut(s) 62, 284, 715, 1287
PpsI GAGTC 4 cut(s) 62, 284, 715, 1287
PscI ACATGT 1 cut(s) 1171
PshAI GACNNNNGTC 1 cut(s) 847
PshBI ATTAAT 1 cut(s) 672
Psp6I CCWGG 4 cut(s) 136, 235, 481, 896
PspFI CCCAGC 1 cut(s) 926
PspGI CCWGG 4 cut(s) 136, 235, 481, 896
PspN4I GGNNCC 4 cut(s) 627, 798, 833, 902
PspPI GGNCC 4 cut(s) 4, 210, 765, 900
PsuI RGATCY 2 cut(s) 423, 572
RsaI GTAC 2 cut(s) 542, 1290
RsaNI GTAC 2 cut(s) 541, 1289
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 5 cut(s) 269, 566, 672, 953, 1101
SatI GCNGC 5 cut(s) 464, 498, 962, 1083, 1086
Sau3AI GATC 6 cut(s) 140, 250, 423, 442, 572, 745
Sau96I GGNCC 4 cut(s) 4, 210, 765, 900
SchI GAGTC 4 cut(s) 62, 285, 715, 1288
ScrFI CCNGG 5 cut(s) 138, 203, 237, 483, 898
SduI GDGCHC 4 cut(s) 604, 628, 788, 1305
SfaNI GCATC 3 cut(s) 85, 162, 1146
SinI GGWCC 4 cut(s) 4, 210, 765, 900
SmiMI CAYNNNNRTG 1 cut(s) 791
SphI GCATGC 1 cut(s) 437
Sse9I AATT 6 cut(s) 193, 258, 676, 711, 738, 1034
SsiI CCGC 5 cut(s) 59, 338, 380, 498, 528
SspMI CTAG 4 cut(s) 335, 1058, 1091, 1232
StyD4I CCNGG 5 cut(s) 136, 201, 235, 481, 896
TaaI ACNGT 5 cut(s) 112, 413, 599, 857, 1295
TaqI TCGA 1 cut(s) 1176
TasI AATT 6 cut(s) 193, 258, 676, 711, 738, 1034
TatI WGTACW 2 cut(s) 540, 1288
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 2 cut(s) 216, 1022
Tru1I TTAA 5 cut(s) 269, 566, 672, 953, 1101
Tru9I TTAA 5 cut(s) 269, 566, 672, 953, 1101
TscAI CASTG 3 cut(s) 19, 604, 1298
TseFI GTSAC 4 cut(s) 533, 849, 911, 1280
TseI GCWGC 4 cut(s) 463, 961, 1082, 1085
Tsp45I GTSAC 4 cut(s) 533, 849, 911, 1280
TspDTI ATGAA 3 cut(s) 806, 1014, 1043
TspGWI ACGGA 2 cut(s) 293, 837
TspRI CASTG 3 cut(s) 19, 604, 1298
Van91I CCANNNNNTGG 2 cut(s) 806, 897
VpaK11BI GGWCC 4 cut(s) 4, 210, 765, 900
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 4 cut(s) 193, 711, 738, 1034
XceI RCATGY 2 cut(s) 437, 1175
XmiI GTMKAC 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 4 cut(s) 335, 1058, 1091, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.