pycom11g00680

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002477
Physical Location & Seq
Forward (+)
508017 .. 511010
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g00680.1

Sequence Viewer

Length: 1314 bp
ATGGACCAGAACACTGAACAGTCTTCCAATGCTCAGAGGGGCTTCAGAGTTCAAGCTCCACTGGTTGACTCCGTATCATGCTATTGTAAAGTAGATGCGGGCTTAAAAACAGTTGCAGGAGCAAGAAAGTTTGTCCCAGGATCAAAAATTTGCATCCAGCCTGATATCAATCCTAATGCGCACAGGGGAAAAAACTTGCGCAGGGAAAGGACTAGAATCCAGCCACCCCTCCTGCCTGGTCTACCTGATGATCTTGCAATTGCTTGTTTAATCAGAGTACCCCGTGTTGAACATAGAAAACTCCGTCTAGTTTGCAAAAGGTGGTATCGCCTTCTAGCTGGAAACTTCTTTTATTCGCTTAGAAAAAGTCTTGGAATGGCAGAAGAATGGGTTTATGTTATCAAAAGAGACCGTGATGGAAGGATCTCTTGGCATGCTTTTGATCCTACATACCAGCTCTGGCAGCCGCTTCCACCCGTTCCTGGTGAGTATTCTGCCGCGCTTGGTTTTGGTTGTGCTGTCCCTAGCGGTTGTCACCTGTACTTGTTTGGAGGAAAAGATCCATTGAAGGGATCTATGAGAAGGGTTATTTTTTACAGTGCTCGGACAAATAAATGGCACAGGGCACCAGATATGCTTCGGAAACGCCATTTCTTTGGCTCTTGTGTTATTAATAATTGTCTTTATGTAGCCGGTGGGGAGTGTGAAGGAATTCAAAGGACTCTTCGTTCTGCTGAAATTTATGATCCGAACAAAAATCGGTGGAGCTTTATTTCCGATATGAGCACAGCTATGGTGCCCTTTATTGGGGTGGTTCATGATGGAATGTGGTTCCTAAAAGGACTTGGGTCACACCGTGAGGTAATGAGTGAAGCCTATACTCCCGAAGCCAATACCTGGACCCCTATTAGTGACGGGATGGTTGCTGGGTGGCGCAACCCGAGTATTTCTTTAAATGGGCAGCTCTATGCTCTGGACTGCCGTGATGGGTGCAAGCTTAGGGTCTATGATCGAGTATCAGATTCATGGATCAAATTTATAGATAGCAAGCTTCATCTAGGGAGTTCTCGTGCTTTGGAGGCTGCTGCACTTGTTCCCCTTAACGGGAAGCTTTGCATTATTCGTAATAACATGAGCATCAGTTTAGTTGATGTTTCAAGTCCGGATAAACATGTCGAAAGCAACCCTCAGCTTTGGGAAAATATCGCCGGTAAAGGTCATTTCAGAACTCTGGTCTCCAATTTATGGTCAAGTATAGCAGGCCGAACTGGTTTGAAGAGTCACATTGTACACTGTCAAGTGCTCCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

438

Amino Acids

49.16

Weight (kDa)

9.54

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 79 - 115 4e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 143 - 303 1.3e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 170 - 346 1e-16 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 190 - 309 9.1e-12 Calicin, beta-propeller domain
Kelch_1 PF01344 216 - 262 3.3e-13 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 180, 200
AccB1I GGYRCC 2 cut(s) 625, 796
AccB7I CCANNNNNTGG 2 cut(s) 897, 1245
AccI GTMKAC 1 cut(s) 241
AccII CGCG 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 1162
AciI CCGC 4 cut(s) 98, 467, 498, 528
AclWI GGATC 7 cut(s) 148, 431, 437, 554, 580, 740, 1037
AcsI RAATTY 4 cut(s) 147, 711, 738, 1034
AcuI CTGAAG 1 cut(s) 28
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 3 cut(s) 279, 542, 1290
AfiI CCNNNNNNNGG 8 cut(s) 482, 569, 806, 807, 897, 1103, 1104, 1245
AflIII ACRYGT 1 cut(s) 1171
AgsI TTSAA 6 cut(s) 53, 290, 568, 716, 1158, 1276
AjnI CCWGG 4 cut(s) 136, 235, 481, 896
AjuI GAANNNNNNNTTGG 2 cut(s) 412, 444
AloI GAACNNNNNNTCC 2 cut(s) 712, 744
Alw21I GWGCWC 3 cut(s) 604, 788, 1305
Alw26I GTCTC 2 cut(s) 402, 1240
AlwI GGATC 7 cut(s) 148, 431, 437, 554, 580, 740, 1037
Ama87I CYCGRG 1 cut(s) 940
Aor13HI TCCGGA 1 cut(s) 1162
AoxI GGCC 1 cut(s) 1261
ApeKI GCWGC 4 cut(s) 463, 961, 1082, 1085
ApoI RAATTY 4 cut(s) 147, 711, 738, 1034
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 4 cut(s) 181, 201, 502, 936
AspS9I GGNCC 2 cut(s) 4, 900
AsuHPI GGTGA 2 cut(s) 497, 527
AvaI CYCGRG 1 cut(s) 940
AvaII GGWCC 2 cut(s) 4, 900
BaeGI GKGCMC 2 cut(s) 628, 801
BanI GGYRCC 2 cut(s) 625, 796
BauI CACGAG 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 3 cut(s) 604, 788, 1305
BbvCI CCTCAGC 1 cut(s) 1188
BbvI GCAGC 4 cut(s) 475, 973, 1069, 1072
BccI CCATC 4 cut(s) 410, 815, 913, 980
BceAI ACGGC 1 cut(s) 966
BciT130I CCWGG 4 cut(s) 138, 237, 483, 898
BcoDI GTCTC 2 cut(s) 402, 1240
BfaI CTAG 5 cut(s) 213, 308, 335, 525, 1058
BisI GCNGC 6 cut(s) 464, 467, 498, 962, 1083, 1086
BlsI GCNGC 6 cut(s) 465, 468, 499, 963, 1084, 1087
Bme1390I CCNGG 4 cut(s) 138, 237, 483, 898
Bme18I GGWCC 2 cut(s) 4, 900
BmeT110I CYCGRG 1 cut(s) 940
BmgT120I GGNCC 2 cut(s) 4, 900
BmiI GGNNCC 4 cut(s) 627, 798, 833, 902
BmrFI CCNGG 4 cut(s) 138, 237, 483, 898
BmsI GCATC 3 cut(s) 85, 162, 1146
BoxI GACNNNNGTC 1 cut(s) 847
BpiI GAAGAC 1 cut(s) 15
Bpu10I CCTNAGC 2 cut(s) 998, 1188
BsaBI GATNNNNATC 1 cut(s) 168
BsaI GGTCTC 2 cut(s) 402, 1240
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 2 cut(s) 1054, 1084
Bsc4I CCNNNNNNNGG 8 cut(s) 482, 569, 806, 807, 897, 1103, 1104, 1245
Bse118I RCCGGY 2 cut(s) 692, 1208
Bse1I ACTGG 2 cut(s) 66, 1273
Bse8I GATNNNNATC 1 cut(s) 168
BseAI TCCGGA 1 cut(s) 1162
BseBI CCWGG 4 cut(s) 138, 237, 483, 898
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 2 cut(s) 153, 924
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 8 cut(s) 482, 569, 806, 807, 897, 1103, 1104, 1245
BseMII CTCAG 2 cut(s) 47, 1202
BseNI ACTGG 2 cut(s) 66, 1273
BseSI GKGCMC 2 cut(s) 628, 801
BseXI GCAGC 4 cut(s) 475, 973, 1069, 1072
BseYI CCCAGC 1 cut(s) 926
BsgI GTGCAG 1 cut(s) 1071
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 1 cut(s) 1263
BshNI GGYRCC 2 cut(s) 625, 796
BsiHKAI GWGCWC 3 cut(s) 604, 788, 1305
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 3 cut(s) 693, 1163, 1209
BslFI GGGAC 2 cut(s) 119, 506
BslI CCNNNNNNNGG 8 cut(s) 482, 569, 806, 807, 897, 1103, 1104, 1245
BsmAI GTCTC 2 cut(s) 402, 1240
BsmFI GGGAC 2 cut(s) 119, 506
BsnI GGCC 1 cut(s) 1263
Bso31I GGTCTC 2 cut(s) 402, 1240
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 5 cut(s) 604, 628, 788, 801, 1305
Bsp13I TCCGGA 1 cut(s) 1162
Bsp1407I TGTACA 1 cut(s) 1288
Bsp143I GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
BspACI CCGC 4 cut(s) 98, 467, 498, 528
BspANI GGCC 1 cut(s) 1263
BspCNI CTCAG 2 cut(s) 46, 1201
BspEI TCCGGA 1 cut(s) 1162
BspFNI CGCG 1 cut(s) 500
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 4 cut(s) 627, 798, 833, 902
BspPI GGATC 7 cut(s) 148, 431, 437, 554, 580, 740, 1037
BspT107I GGYRCC 2 cut(s) 625, 796
BspTNI GGTCTC 2 cut(s) 402, 1240
BsrFI RCCGGY 2 cut(s) 692, 1208
BsrGI TGTACA 1 cut(s) 1288
BsrI ACTGG 2 cut(s) 66, 1273
BssAI RCCGGY 2 cut(s) 692, 1208
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
BssSI CACGAG 1 cut(s) 1068
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 4 cut(s) 138, 237, 483, 898
Bst4CI ACNGT 6 cut(s) 21, 112, 413, 599, 857, 1295
Bst6I CTCTTC 2 cut(s) 729, 1271
BstAUI TGTACA 1 cut(s) 1288
BstC8I GCNNGC 5 cut(s) 100, 435, 995, 1049, 1261
BstDEI CTNAG 4 cut(s) 33, 359, 998, 1188
BstF5I GGATG 2 cut(s) 153, 924
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 4 cut(s) 181, 201, 502, 936
BstKTI GATC 9 cut(s) 143, 253, 426, 445, 562, 575, 748, 1012, 1032
BstMAI GTCTC 2 cut(s) 402, 1240
BstMBI GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
BstMWI GCNNNNNNNGC 2 cut(s) 463, 1079
BstNI CCWGG 4 cut(s) 138, 237, 483, 898
BstNSI RCATGY 2 cut(s) 437, 1175
BstPAI GACNNNNGTC 1 cut(s) 847
BstSCI CCNGG 4 cut(s) 136, 235, 481, 896
BstSLI GKGCMC 2 cut(s) 628, 801
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 4 cut(s) 475, 973, 1069, 1072
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 3 cut(s) 423, 559, 572
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 3 cut(s) 423, 559, 572
BsuRI GGCC 1 cut(s) 1263
BtsCI GGATG 2 cut(s) 153, 924
BtsIMutI CAGTG 4 cut(s) 12, 59, 604, 1291
Cac8I GCNNGC 5 cut(s) 100, 435, 995, 1049, 1261
CciI TCATGA 1 cut(s) 817
CfoI GCGC 4 cut(s) 181, 201, 502, 936
Cfr10I RCCGGY 2 cut(s) 692, 1208
Cfr13I GGNCC 2 cut(s) 4, 900
Csp6I GTAC 3 cut(s) 278, 541, 1289
CviAII CATG 7 cut(s) 78, 434, 818, 1026, 1132, 1172, 1311
CviQI GTAC 3 cut(s) 278, 541, 1289
DdeI CTNAG 4 cut(s) 33, 359, 998, 1188
DpnI GATC 9 cut(s) 142, 252, 425, 444, 561, 574, 747, 1011, 1031
DpnII GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
DraI TTTAAA 1 cut(s) 954
DraIII CACNNNGTG 1 cut(s) 857
Eam1104I CTCTTC 2 cut(s) 729, 1271
EarI CTCTTC 2 cut(s) 729, 1271
Eco31I GGTCTC 2 cut(s) 402, 1240
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 2 cut(s) 4, 900
Eco57I CTGAAG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 940
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 4 cut(s) 136, 235, 481, 896
EcoRV GATATC 1 cut(s) 166
FaeI CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FaqI GGGAC 2 cut(s) 119, 506
FatI CATG 7 cut(s) 77, 433, 817, 1025, 1131, 1171, 1310
FauI CCCGC 1 cut(s) 91
FblI GTMKAC 1 cut(s) 241
Fnu4HI GCNGC 6 cut(s) 464, 467, 498, 962, 1083, 1086
FokI GGATG 2 cut(s) 140, 931
Fsp4HI GCNGC 6 cut(s) 464, 467, 498, 962, 1083, 1086
FspAI RTGCGCAY 1 cut(s) 180
FspBI CTAG 5 cut(s) 213, 308, 335, 525, 1058
FspI TGCGCA 2 cut(s) 180, 200
GlaI GCGC 4 cut(s) 180, 200, 501, 935
GluI GCNGC 6 cut(s) 464, 467, 498, 962, 1083, 1086
GsaI CCCAGC 1 cut(s) 930
HaeIII GGCC 1 cut(s) 1263
HapII CCGG 3 cut(s) 693, 1163, 1209
HhaI GCGC 4 cut(s) 181, 201, 502, 936
Hin1II CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
Hin6I GCGC 4 cut(s) 179, 199, 500, 934
HinP1I GCGC 4 cut(s) 179, 199, 500, 934
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 3 cut(s) 995, 1049, 1109
HinfI GANTC 5 cut(s) 68, 216, 721, 1022, 1279
HpaII CCGG 3 cut(s) 693, 1163, 1209
HphI GGTGA 2 cut(s) 497, 527
Hpy166II GTNNAC 3 cut(s) 67, 242, 1291
Hpy188I TCNGA 9 cut(s) 36, 47, 275, 606, 642, 750, 778, 1021, 1226
Hpy188III TCNNGA 4 cut(s) 818, 884, 974, 1163
Hpy8I GTNNAC 3 cut(s) 67, 242, 1291
HpyAV CCTTC 5 cut(s) 341, 414, 562, 576, 701
HpyCH4III ACNGT 6 cut(s) 21, 112, 413, 599, 857, 1295
HpyCH4V TGCA 7 cut(s) 116, 153, 257, 315, 993, 1088, 1116
HpyF10VI GCNNNNNNNGC 2 cut(s) 463, 1079
HpyF3I CTNAG 4 cut(s) 33, 359, 998, 1188
Hsp92II CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
HspAI GCGC 4 cut(s) 179, 199, 500, 934
Kpn2I TCCGGA 1 cut(s) 1162
Kzo9I GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
LmnI GCTCC 4 cut(s) 61, 119, 765, 1308
Lsp1109I GCAGC 4 cut(s) 475, 973, 1069, 1072
LweI GCATC 3 cut(s) 85, 162, 1146
MaeI CTAG 5 cut(s) 213, 308, 335, 525, 1058
MaeIII GTNAC 4 cut(s) 533, 849, 911, 1280
MalI GATC 9 cut(s) 142, 252, 425, 444, 561, 574, 747, 1011, 1031
MboI GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
MboII GAAGA 4 cut(s) 15, 395, 716, 1288
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 3 cut(s) 423, 559, 572
MhlI GDGCHC 5 cut(s) 604, 628, 788, 801, 1305
MluCI AATT 7 cut(s) 147, 258, 676, 711, 738, 1034, 1240
MlyI GAGTC 3 cut(s) 62, 715, 1288
MnlI CCTC 6 cut(s) 30, 239, 545, 853, 1072, 1197
MroI TCCGGA 1 cut(s) 1162
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 5 cut(s) 104, 269, 672, 953, 1101
MslI CAYNNNNRTG 1 cut(s) 791
MspI CCGG 3 cut(s) 693, 1163, 1209
MspR9I CCNGG 4 cut(s) 138, 237, 483, 898
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 4 cut(s) 138, 237, 483, 898
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 2 cut(s) 463, 1079
NdeII GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
NlaIII CATG 7 cut(s) 81, 437, 821, 1029, 1135, 1175, 1314
NlaIV GGNNCC 4 cut(s) 627, 798, 833, 902
NmuCI GTSAC 4 cut(s) 533, 849, 911, 1280
NsbI TGCGCA 2 cut(s) 180, 200
NspI RCATGY 2 cut(s) 437, 1175
PaeI GCATGC 1 cut(s) 437
PagI TCATGA 1 cut(s) 817
PciI ACATGT 1 cut(s) 1171
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 2 cut(s) 216, 1022
PflMI CCANNNNNTGG 2 cut(s) 897, 1245
PkrI GCNGC 6 cut(s) 465, 468, 499, 963, 1084, 1087
PleI GAGTC 3 cut(s) 62, 715, 1287
PpsI GAGTC 3 cut(s) 62, 715, 1287
PscI ACATGT 1 cut(s) 1171
PshAI GACNNNNGTC 1 cut(s) 847
PshBI ATTAAT 1 cut(s) 672
Psp6I CCWGG 4 cut(s) 136, 235, 481, 896
PspFI CCCAGC 1 cut(s) 926
PspGI CCWGG 4 cut(s) 136, 235, 481, 896
PspN4I GGNNCC 4 cut(s) 627, 798, 833, 902
PspPI GGNCC 2 cut(s) 4, 900
PsuI RGATCY 3 cut(s) 423, 559, 572
RsaI GTAC 3 cut(s) 279, 542, 1290
RsaNI GTAC 3 cut(s) 278, 541, 1289
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 5 cut(s) 104, 269, 672, 953, 1101
SatI GCNGC 6 cut(s) 464, 467, 498, 962, 1083, 1086
Sau3AI GATC 9 cut(s) 140, 250, 423, 442, 559, 572, 745, 1009, 1029
Sau96I GGNCC 2 cut(s) 4, 900
SchI GAGTC 3 cut(s) 62, 715, 1288
ScrFI CCNGG 4 cut(s) 138, 237, 483, 898
SduI GDGCHC 5 cut(s) 604, 628, 788, 801, 1305
SfaNI GCATC 3 cut(s) 85, 162, 1146
SinI GGWCC 2 cut(s) 4, 900
SmiMI CAYNNNNRTG 1 cut(s) 791
SphI GCATGC 1 cut(s) 437
Sse9I AATT 7 cut(s) 147, 258, 676, 711, 738, 1034, 1240
SsiI CCGC 4 cut(s) 98, 467, 498, 528
SspMI CTAG 5 cut(s) 213, 308, 335, 525, 1058
StyD4I CCNGG 4 cut(s) 136, 235, 481, 896
TaaI ACNGT 6 cut(s) 21, 112, 413, 599, 857, 1295
TaqI TCGA 2 cut(s) 1012, 1176
TasI AATT 7 cut(s) 147, 258, 676, 711, 738, 1034, 1240
TatI WGTACW 2 cut(s) 540, 1288
TauI GCSGC 2 cut(s) 469, 500
TfiI GAWTC 2 cut(s) 216, 1022
Tru1I TTAA 5 cut(s) 104, 269, 672, 953, 1101
Tru9I TTAA 5 cut(s) 104, 269, 672, 953, 1101
TscAI CASTG 4 cut(s) 19, 66, 604, 1298
TseFI GTSAC 4 cut(s) 533, 849, 911, 1280
TseI GCWGC 4 cut(s) 463, 961, 1082, 1085
Tsp45I GTSAC 4 cut(s) 533, 849, 911, 1280
TspDTI ATGAA 3 cut(s) 806, 1014, 1043
TspGWI ACGGA 2 cut(s) 61, 293
TspRI CASTG 4 cut(s) 19, 66, 604, 1298
Van91I CCANNNNNTGG 2 cut(s) 897, 1245
VpaK11BI GGWCC 2 cut(s) 4, 900
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 4 cut(s) 147, 711, 738, 1034
XceI RCATGY 2 cut(s) 437, 1175
XmiI GTMKAC 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 5 cut(s) 213, 308, 335, 525, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.