RchiOBHm_Chr5g0082431

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002477
Physical Location & Seq
Reverse (-)
88422721 .. 88423455
735 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35664

Sequence Viewer

Length: 735 bp
ATGTCAAGCATCCTGACATGGCTAGTTTTGCAGGTTGACTCCGTATCATGCTATTGCAAAGTAGATGCAGGCTTAAAAACAGTTGCAGGGGCAAGAAAGTTTTTCCCAGGATCAAAACTTTGTATCCAGCCTGATATCAATCCAAACTCACACAAGGGGAAAAGTTCACGTAGGGAAAGGACGAGAATCCAGCCACCTCTTCTGCCTGGCCTACCTGATGATCTTGCAATTGCTTGTTTAATCAGAGTCCCTCGGGTGGAGCATAGAAAACTCTGTCTAGTTTGCAAAAGATGGTATCGCCTTCTGGCTGGAAACTTCTTTTATTCTCTTAGGAAAAGTCTTGGAATGGCAGAAGAGTGGGTTTATGTCATCAAAAGAGACCGCAATGGAAGAATCTCTTGGCATGCTTTTGATCCCACGTACCAGCTCTGGCAGCCACTTCCGCCCGTTCCTGGTGAATATTCTGCAGCGCTTGGTTTTGGTTGTGCTGTCCTTAGTGGTTGCCACTTGTACTTATTTGGAGGAAAAGATCCATTGAAGGGATCTATGAGACAGGTTATTTTTTACAGTGCTCGGACAAATAAATGGCACAGGGCACCAGATATGCTTCGAAAACGGCATTTCTTTGGTGCTTGTGTTATTAATAACTGCCTTTATGTAGCTGGTGGAGAGTGTGAAGGAATACAAAGGACTCTCCGTTCTGCTGAAATTTATGACCCGAACAAGAACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

244

Amino Acids

27.75

Weight (kDa)

9.72

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 69 - 105 5.9e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 133 - 242 1.3e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 160 - 242 5.1e-10 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 174 - 243 4.2e-07 Calicin, beta-propeller domain
Kelch_1 PF01344 206 - 243 2.8e-09 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 595
AciI CCGC 2 cut(s) 382, 443
AclWI GGATC 4 cut(s) 118, 407, 524, 550
AcsI RAATTY 1 cut(s) 708
AfaI GTAC 2 cut(s) 422, 512
AfeI AGCGCT 1 cut(s) 471
AfiI CCNNNNNNNGG 3 cut(s) 256, 452, 539
AgeI ACCGGT 1 cut(s) 729
AgsI TTSAA 1 cut(s) 538
AjnI CCWGG 3 cut(s) 106, 205, 451
AjuI GAANNNNNNNTTGG 2 cut(s) 382, 414
AloI GAACNNNNNNTCC 2 cut(s) 682, 714
AluBI AGCT 2 cut(s) 427, 662
AluI AGCT 2 cut(s) 427, 662
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 2 cut(s) 372, 544
AlwI GGATC 4 cut(s) 118, 407, 524, 550
Ama87I CYCGRG 1 cut(s) 252
Aor51HI AGCGCT 1 cut(s) 471
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 2 cut(s) 433, 467
ApoI RAATTY 1 cut(s) 708
AseI ATTAAT 1 cut(s) 642
AsiGI ACCGGT 1 cut(s) 729
AspLEI GCGC 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 467
AsuII TTCGAA 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 595
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 2 cut(s) 445, 479
BccI CCATC 1 cut(s) 285
BceAI ACGGC 1 cut(s) 632
BciT130I CCWGG 3 cut(s) 108, 207, 453
BciVI GTATCC 1 cut(s) 134
BcoDI GTCTC 2 cut(s) 372, 544
BfaI CTAG 2 cut(s) 23, 278
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 22
BfuI GTATCC 1 cut(s) 134
BisI GCNGC 2 cut(s) 434, 468
BlsI GCNGC 2 cut(s) 435, 469
Bme1390I CCNGG 3 cut(s) 108, 207, 453
BmeT110I CYCGRG 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 597
BmrFI CCNGG 3 cut(s) 108, 207, 453
BmsI GCATC 2 cut(s) 18, 55
Bpu14I TTCGAA 1 cut(s) 610
BsaAI YACGTR 2 cut(s) 170, 420
BsaBI GATNNNNATC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 106, 251
BsaWI WCCGGW 1 cut(s) 729
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 452, 539
Bse118I RCCGGY 1 cut(s) 729
Bse3DI GCAATG 1 cut(s) 391
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 3 cut(s) 108, 207, 453
BseDI CCNNGG 2 cut(s) 106, 251
BseGI GGATG 1 cut(s) 9
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 3 cut(s) 256, 452, 539
BseMI GCAATG 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 2 cut(s) 445, 479
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 595
BshTI ACCGGT 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 574
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 1 cut(s) 730
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 3 cut(s) 256, 452, 539
BsmAI GTCTC 2 cut(s) 372, 544
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 1 cut(s) 372
BsoBI CYCGRG 1 cut(s) 252
Bsp119I TTCGAA 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 574, 598
Bsp143I GATC 5 cut(s) 110, 220, 412, 529, 542
BspACI CCGC 2 cut(s) 382, 443
BspANI GGCC 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 597
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 4 cut(s) 118, 407, 524, 550
BspT104I TTCGAA 1 cut(s) 610
BspT107I GGYRCC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 1 cut(s) 729
BssAI RCCGGY 1 cut(s) 729
BssECI CCNNGG 2 cut(s) 106, 251
BssMI GATC 5 cut(s) 110, 220, 412, 529, 542
Bst2UI CCWGG 3 cut(s) 108, 207, 453
Bst4CI ACNGT 2 cut(s) 82, 569
Bst6I CTCTTC 2 cut(s) 204, 348
BstBAI YACGTR 2 cut(s) 170, 420
BstBI TTCGAA 1 cut(s) 610
BstC8I GCNNGC 2 cut(s) 70, 405
BstDEI CTNAG 2 cut(s) 329, 494
BstF5I GGATG 1 cut(s) 9
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 5 cut(s) 113, 223, 415, 532, 545
BstMAI GTCTC 2 cut(s) 372, 544
BstMBI GATC 5 cut(s) 110, 220, 412, 529, 542
BstMWI GCNNNNNNNGC 3 cut(s) 28, 433, 442
BstNI CCWGG 3 cut(s) 108, 207, 453
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 3 cut(s) 106, 205, 451
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 2 cut(s) 445, 479
BstX2I RGATCY 2 cut(s) 529, 542
BstYI RGATCY 2 cut(s) 529, 542
BsuI GTATCC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 210
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 574
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 2 cut(s) 70, 405
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 729
Csp6I GTAC 2 cut(s) 421, 511
CspAI ACCGGT 1 cut(s) 729
CviAII CATG 3 cut(s) 18, 48, 404
CviJI RGCY 9 cut(s) 22, 72, 130, 193, 210, 308, 427, 436, 662
CviKI_1 RGCY 9 cut(s) 22, 72, 130, 193, 210, 308, 427, 436, 662
CviQI GTAC 2 cut(s) 421, 511
DdeI CTNAG 2 cut(s) 329, 494
DpnI GATC 5 cut(s) 112, 222, 414, 531, 544
DpnII GATC 5 cut(s) 110, 220, 412, 529, 542
Eam1104I CTCTTC 2 cut(s) 204, 348
EarI CTCTTC 2 cut(s) 204, 348
EciI GGCGGA 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 372
Eco32I GATATC 1 cut(s) 136
Eco47III AGCGCT 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 252
EcoRII CCWGG 3 cut(s) 106, 205, 451
EcoRV GATATC 1 cut(s) 136
FaeI CATG 3 cut(s) 21, 51, 407
FaiI YATR 9 cut(s) 19, 49, 264, 366, 405, 548, 605, 657, 714
FalI AAGNNNNNCTT 2 cut(s) 382, 414
FaqI GGGAC 1 cut(s) 233
FatI CATG 3 cut(s) 17, 47, 403
Fnu4HI GCNGC 2 cut(s) 434, 468
Fsp4HI GCNGC 2 cut(s) 434, 468
FspBI CTAG 2 cut(s) 23, 278
GlaI GCGC 1 cut(s) 471
GluI GCNGC 2 cut(s) 434, 468
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 730
HhaI GCGC 1 cut(s) 472
Hin1II CATG 3 cut(s) 21, 51, 407
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 5 cut(s) 38, 186, 246, 393, 691
HpaII CCGG 1 cut(s) 730
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 2 cut(s) 37, 167
Hpy188I TCNGA 2 cut(s) 245, 576
Hpy188III TCNNGA 1 cut(s) 13
Hpy8I GTNNAC 2 cut(s) 37, 167
HpyAV CCTTC 3 cut(s) 311, 532, 671
HpyCH4III ACNGT 2 cut(s) 82, 569
HpyCH4IV ACGT 2 cut(s) 169, 419
HpyCH4V TGCA 7 cut(s) 31, 57, 68, 86, 227, 285, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 433, 442
HpyF3I CTNAG 2 cut(s) 329, 494
HpySE526I ACGT 2 cut(s) 169, 419
Hsp92II CATG 3 cut(s) 21, 51, 407
HspAI GCGC 1 cut(s) 470
Kzo9I GATC 5 cut(s) 110, 220, 412, 529, 542
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 2 cut(s) 445, 479
LweI GCATC 2 cut(s) 18, 55
MaeI CTAG 2 cut(s) 23, 278
MaeII ACGT 2 cut(s) 169, 419
MalI GATC 5 cut(s) 112, 222, 414, 531, 544
MboI GATC 5 cut(s) 110, 220, 412, 529, 542
MboII GAAGA 3 cut(s) 191, 365, 402
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 2 cut(s) 529, 542
MhlI GDGCHC 2 cut(s) 574, 598
MluCI AATT 2 cut(s) 228, 708
MlyI GAGTC 3 cut(s) 32, 255, 685
MnlI CCTC 3 cut(s) 207, 261, 515
MseI TTAA 3 cut(s) 74, 239, 642
MspI CCGG 1 cut(s) 730
MspR9I CCNGG 3 cut(s) 108, 207, 453
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 3 cut(s) 108, 207, 453
MwoI GCNNNNNNNGC 3 cut(s) 28, 433, 442
NdeII GATC 5 cut(s) 110, 220, 412, 529, 542
NlaIII CATG 3 cut(s) 21, 51, 407
NlaIV GGNNCC 1 cut(s) 597
NspI RCATGY 1 cut(s) 407
NspV TTCGAA 1 cut(s) 610
PaeI GCATGC 1 cut(s) 407
PfeI GAWTC 2 cut(s) 186, 393
PinAI ACCGGT 1 cut(s) 729
PkrI GCNGC 2 cut(s) 435, 469
PleI GAGTC 3 cut(s) 32, 254, 685
PpsI GAGTC 3 cut(s) 32, 254, 685
Ppu21I YACGTR 2 cut(s) 170, 420
PshBI ATTAAT 1 cut(s) 642
Psp6I CCWGG 3 cut(s) 106, 205, 451
PspGI CCWGG 3 cut(s) 106, 205, 451
PspN4I GGNNCC 1 cut(s) 597
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 2 cut(s) 529, 542
RsaI GTAC 2 cut(s) 422, 512
RsaNI GTAC 2 cut(s) 421, 511
SaqAI TTAA 3 cut(s) 74, 239, 642
SatI GCNGC 2 cut(s) 434, 468
Sau3AI GATC 5 cut(s) 110, 220, 412, 529, 542
SchI GAGTC 3 cut(s) 32, 255, 685
ScrFI CCNGG 3 cut(s) 108, 207, 453
SduI GDGCHC 2 cut(s) 574, 598
SetI ASST 8 cut(s) 36, 172, 199, 217, 422, 429, 558, 664
SfaNI GCATC 2 cut(s) 18, 55
SfcI CTRYAG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 610
SphI GCATGC 1 cut(s) 407
Sse9I AATT 2 cut(s) 228, 708
SsiI CCGC 2 cut(s) 382, 443
SspI AATATT 1 cut(s) 461
SspMI CTAG 2 cut(s) 23, 278
StyD4I CCNGG 3 cut(s) 106, 205, 451
TaaI ACNGT 2 cut(s) 82, 569
TaiI ACGT 2 cut(s) 172, 422
TaqI TCGA 1 cut(s) 610
TasI AATT 2 cut(s) 228, 708
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 2 cut(s) 186, 393
Tru1I TTAA 3 cut(s) 74, 239, 642
Tru9I TTAA 3 cut(s) 74, 239, 642
TscAI CASTG 1 cut(s) 574
TseI GCWGC 2 cut(s) 433, 467
TspGWI ACGGA 2 cut(s) 31, 686
TspRI CASTG 1 cut(s) 574
VspI ATTAAT 1 cut(s) 642
XapI RAATTY 1 cut(s) 708
XceI RCATGY 1 cut(s) 407
XspI CTAG 2 cut(s) 23, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.