MD02G1034600.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002477
Physical Location & Seq
Reverse (-)
2644554 .. 2647609
3056 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1034600.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGACACAAGAATACAGCCTCCGCTGGTTGATACAACAGCATGCTTATGTAGAGTAGATGCTGGCCTTAAAAGTGTTGCTGGAGCTAAAAAGTATGTCCCTGGGGCAAAGCTCTGTCTTCAACCCGATACTAAATCATCCATTCATCCAACTAGGAGTAAGCCTTCACGTGGTGAGAGTAGCCGCATTCAATCCCCTCTGCTTCCTGGACTCCCAGATGATCTTGCTGTTGCTTGCCTAGTTCGGGTCCCAAGAGTCGAGCATCGCAAGCTCCGCCTGGTTTGCAAAAGATGGTTCCGTCTTTTGGCTGCTAACTTCTTTTACTCGCTCCGTAGGAACCTTGGCATTGCAGAAGAATGGATATATCTAATTAAGAGAGATCGAGAAGGGAAAATCACTTGGCATGCTTTTGATCCCGTGTGCCAGCTCTGGCAACCCCTCCCTCCTGTCCCTAAGGAATATTCTGCAGCCCTAGGGTTTGGTTGTGCTGTACTCGGTGGCTGTCACCTGTATTTATTTGGAGGAAAAGATCCGCTGAAGGGATCAATGAGGCGAGTCATTTTTTATAATGCCAGGACCAATAAATGGCACCGTGCCCCAGATATGCTTCGGAGGCGGCATTTCTTTGGCTCGTGTGTTATAAATAACTGCCTGTATGTTGCCGGCGGGGAGAATGAGGGAGTAAATCGGTCTCTGAAATCAGCTGAAGTCTATGATCCGAACAAGAACAGATGGTCCTTCATTTCGGAGATGAGCACAGCAATGGTTCCCTTCATTGGGGTTGTTTACGAAGGCAAATGGTTCTTGAAGGGTTTAGGGTCTCATCGACAAGTTTTGAGCGAGGTCTACCAACCAGAAAACGACACCTGGAACTCTGTGTATGACGGAATGGTTGCAGGCTGGAGAAATCCTAGTGCTTCTCTAAACAGCCATCTCTATGCTTTGGAATGCAAAGATGGCTGCAAACTTAGAGTCTATGACGAAGCGACGGATTCTTGGAGCAAGCACATTGACAGTAAGATGCATTTGGGTAATTCTAGGGCTTTGGAGGCAGCTTCCCTCGTTCCCCTCAACGGTAAACTTTGCATCGTTCGGAATAACATGAGCATCTCGCTGGTTGATGTTTCGAACTCCAGTGATGTTCACGGGGAAAGTGCCGAGCATCTGTGGGAAACTATAGCAGGTAAAGGGCAGTTCAAGACCTTGGTTACGAATCTGTGGTCAAGCCTTGCCGGCAGGGGCCGCCTTAAAAGTCACATAGTTCACTGCCAGGTTCTTCAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

429

Amino Acids

48.02

Weight (kDa)

9.4

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 132 - 293 5e-12 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 149 - 339 2.6e-21 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 180 - 292 1.1e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 205 - 252 4.3e-15 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 567, 641
Acc36I ACCTGC 1 cut(s) 1172
AccB1I GGYRCC 1 cut(s) 588
AccB7I CCANNNNNTGG 1 cut(s) 585
AccI GTMKAC 1 cut(s) 846
AciI CCGC 7 cut(s) 23, 184, 274, 533, 616, 666, 1243
AclWI GGATC 4 cut(s) 407, 524, 550, 710
AcuI CTGAAG 2 cut(s) 557, 726
AcvI CACGTG 1 cut(s) 170
AdeI CACNNNGTG 1 cut(s) 173
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 8 cut(s) 170, 244, 304, 539, 585, 776, 777, 1073
AgsI TTSAA 5 cut(s) 122, 191, 808, 1198, 1280
AjnI CCWGG 6 cut(s) 100, 205, 276, 572, 866, 1269
AloI GAACNNNNNNTCC 2 cut(s) 719, 751
AluBI AGCT 6 cut(s) 86, 112, 271, 427, 704, 1055
AluI AGCT 6 cut(s) 86, 112, 271, 427, 704, 1055
Alw21I GWGCWC 1 cut(s) 758
Alw26I GTCTC 2 cut(s) 696, 825
AlwI GGATC 4 cut(s) 407, 524, 550, 710
AoxI GGCC 2 cut(s) 64, 1240
ApeKI GCWGC 4 cut(s) 308, 467, 960, 1052
ArsI GACNNNNNNTTYG 4 cut(s) 957, 989, 1205, 1237
AspA2I CCTAGG 1 cut(s) 472
AspS9I GGNCC 4 cut(s) 247, 576, 735, 1240
AsuHPI GGTGA 2 cut(s) 185, 497
AsuII TTCGAA 1 cut(s) 1127
AvaII GGWCC 3 cut(s) 247, 576, 735
AvrII CCTAGG 1 cut(s) 472
AxyI CCTNAGG 1 cut(s) 453
BaeGI GKGCMC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 588
BauI CACGAG 1 cut(s) 631
BbrPI CACGTG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 758
BbvI GCAGC 4 cut(s) 295, 479, 947, 1064
BccI CCATC 4 cut(s) 285, 726, 939, 950
BciT130I CCWGG 6 cut(s) 102, 207, 278, 574, 868, 1271
BcoDI GTCTC 2 cut(s) 696, 825
BfaI CTAG 5 cut(s) 153, 239, 473, 912, 1038
BfmI CTRYAG 2 cut(s) 465, 1176
BfuAI ACCTGC 1 cut(s) 1172
BglI GCCNNNNNGGC 1 cut(s) 1233
BisI GCNGC 7 cut(s) 184, 309, 468, 617, 961, 1053, 1243
BlnI CCTAGG 1 cut(s) 472
BlsI GCNGC 7 cut(s) 185, 310, 469, 618, 962, 1054, 1244
Bme1390I CCNGG 6 cut(s) 102, 207, 278, 574, 868, 1271
Bme18I GGWCC 3 cut(s) 247, 576, 735
BmgT120I GGNCC 4 cut(s) 247, 576, 735, 1240
BmiI GGNNCC 7 cut(s) 248, 249, 296, 338, 590, 768, 1241
BmrFI CCNGG 6 cut(s) 102, 207, 278, 574, 868, 1271
BmsI GCATC 6 cut(s) 49, 271, 1011, 1095, 1116, 1171
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 3 cut(s) 102, 922, 1117
Bpu14I TTCGAA 1 cut(s) 1127
BsaAI YACGTR 1 cut(s) 170
BsaI GGTCTC 2 cut(s) 696, 825
BsaJI CCNNGG 5 cut(s) 100, 101, 340, 472, 1203
Bsc4I CCNNNNNNNGG 8 cut(s) 170, 244, 304, 539, 585, 776, 777, 1073
Bse118I RCCGGY 2 cut(s) 662, 1232
Bse1I ACTGG 1 cut(s) 1134
Bse21I CCTNAGG 1 cut(s) 453
Bse3DI GCAATG 2 cut(s) 345, 768
BseBI CCWGG 6 cut(s) 102, 207, 278, 574, 868, 1271
BseDI CCNNGG 5 cut(s) 100, 101, 340, 472, 1203
BseGI GGATG 2 cut(s) 137, 145
BseLI CCNNNNNNNGG 8 cut(s) 170, 244, 304, 539, 585, 776, 777, 1073
BseMI GCAATG 2 cut(s) 345, 768
BseNI ACTGG 1 cut(s) 1134
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 4 cut(s) 295, 479, 947, 1064
BshFI GGCC 2 cut(s) 66, 1242
BshNI GGYRCC 1 cut(s) 588
BsiHKAI GWGCWC 1 cut(s) 758
BsiSI CCGG 2 cut(s) 663, 1233
BslFI GGGAC 3 cut(s) 83, 233, 434
BslI CCNNNNNNNGG 8 cut(s) 170, 244, 304, 539, 585, 776, 777, 1073
BsmAI GTCTC 2 cut(s) 696, 825
BsmFI GGGAC 3 cut(s) 83, 233, 434
BsmI GAATGC 2 cut(s) 186, 953
BsnI GGCC 2 cut(s) 66, 1242
Bso31I GGTCTC 2 cut(s) 696, 825
Bsp119I TTCGAA 1 cut(s) 1127
Bsp1286I GDGCHC 2 cut(s) 598, 758
Bsp143I GATC 6 cut(s) 220, 379, 412, 529, 542, 715
BspACI CCGC 7 cut(s) 23, 184, 274, 533, 616, 666, 1243
BspANI GGCC 2 cut(s) 66, 1242
BspLI GGNNCC 7 cut(s) 248, 249, 296, 338, 590, 768, 1241
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 1172
BspPI GGATC 4 cut(s) 407, 524, 550, 710
BspT104I TTCGAA 1 cut(s) 1127
BspT107I GGYRCC 1 cut(s) 588
BspTNI GGTCTC 2 cut(s) 696, 825
BsrDI GCAATG 2 cut(s) 345, 768
BsrFI RCCGGY 2 cut(s) 662, 1232
BsrI ACTGG 1 cut(s) 1134
BssAI RCCGGY 2 cut(s) 662, 1232
BssECI CCNNGG 5 cut(s) 100, 101, 340, 472, 1203
BssMI GATC 6 cut(s) 220, 379, 412, 529, 542, 715
BssSI CACGAG 1 cut(s) 631
BssT1I CCWWGG 3 cut(s) 340, 472, 1203
Bst2BI CACGAG 1 cut(s) 631
Bst2UI CCWGG 6 cut(s) 102, 207, 278, 574, 868, 1271
Bst4CI ACNGT 3 cut(s) 593, 1016, 1076
BstBAI YACGTR 1 cut(s) 170
BstBI TTCGAA 1 cut(s) 1127
BstDEI CTNAG 2 cut(s) 453, 968
BstF5I GGATG 2 cut(s) 137, 145
BstKTI GATC 6 cut(s) 223, 382, 415, 532, 545, 718
BstMAI GTCTC 2 cut(s) 696, 825
BstMBI GATC 6 cut(s) 220, 379, 412, 529, 542, 715
BstMWI GCNNNNNNNGC 8 cut(s) 268, 273, 282, 613, 957, 1049, 1233, 1242
BstNI CCWGG 6 cut(s) 102, 207, 278, 574, 868, 1271
BstNSI RCATGY 2 cut(s) 45, 407
BstSCI CCNGG 6 cut(s) 100, 205, 276, 572, 866, 1269
BstSFI CTRYAG 2 cut(s) 465, 1176
BstSLI GKGCMC 1 cut(s) 598
BstV1I GCAGC 4 cut(s) 295, 479, 947, 1064
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
Bsu36I CCTNAGG 1 cut(s) 453
BsuRI GGCC 2 cut(s) 66, 1242
BtgZI GCGATG 1 cut(s) 248
BtsCI GGATG 2 cut(s) 137, 145
BtsI GCAGTG 1 cut(s) 1264
BtsIMutI CAGTG 2 cut(s) 1141, 1264
BveI ACCTGC 1 cut(s) 1172
Cfr10I RCCGGY 2 cut(s) 662, 1232
Cfr13I GGNCC 4 cut(s) 247, 576, 735, 1240
Csp6I GTAC 1 cut(s) 491
CviAII CATG 3 cut(s) 42, 404, 1102
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 2 cut(s) 453, 968
DpnI GATC 6 cut(s) 222, 381, 414, 531, 544, 717
DpnII GATC 6 cut(s) 220, 379, 412, 529, 542, 715
DraIII CACNNNGTG 1 cut(s) 173
EciI GGCGGA 1 cut(s) 263
Eco130I CCWWGG 3 cut(s) 340, 472, 1203
Eco31I GGTCTC 2 cut(s) 696, 825
Eco47I GGWCC 3 cut(s) 247, 576, 735
Eco57I CTGAAG 2 cut(s) 557, 726
Eco72I CACGTG 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 6 cut(s) 100, 205, 276, 572, 866, 1269
EcoT14I CCWWGG 3 cut(s) 340, 472, 1203
EcoT22I ATGCAT 1 cut(s) 1026
ErhI CCWWGG 3 cut(s) 340, 472, 1203
FaeI CATG 3 cut(s) 45, 407, 1105
FaqI GGGAC 3 cut(s) 83, 233, 434
FatI CATG 3 cut(s) 41, 403, 1101
FauI CCCGC 1 cut(s) 659
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 7 cut(s) 184, 309, 468, 617, 961, 1053, 1243
FokI GGATG 2 cut(s) 124, 132
Fsp4HI GCNGC 7 cut(s) 184, 309, 468, 617, 961, 1053, 1243
FspBI CTAG 5 cut(s) 153, 239, 473, 912, 1038
GluI GCNGC 7 cut(s) 184, 309, 468, 617, 961, 1053, 1243
GsuI CTGGAG 3 cut(s) 102, 922, 1117
HaeIII GGCC 2 cut(s) 66, 1242
HapII CCGG 2 cut(s) 663, 1233
Hin1II CATG 3 cut(s) 45, 407, 1105
HinfI GANTC 6 cut(s) 210, 255, 555, 972, 992, 1213
HpaII CCGG 2 cut(s) 663, 1233
HphI GGTGA 2 cut(s) 185, 497
Hpy166II GTNNAC 5 cut(s) 787, 847, 1079, 1144, 1264
Hpy188I TCNGA 5 cut(s) 612, 696, 720, 748, 1095
Hpy188III TCNNGA 3 cut(s) 383, 805, 1198
Hpy8I GTNNAC 5 cut(s) 787, 847, 1079, 1144, 1264
Hpy99I CGWCG 1 cut(s) 991
HpyAV CCTTC 7 cut(s) 174, 380, 532, 748, 781, 785, 802
HpyCH4III ACNGT 3 cut(s) 593, 1016, 1076
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 8 cut(s) 285, 350, 467, 896, 951, 963, 1024, 1086
HpyF10VI GCNNNNNNNGC 8 cut(s) 268, 273, 282, 613, 957, 1049, 1233, 1242
HpyF3I CTNAG 2 cut(s) 453, 968
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 3 cut(s) 45, 407, 1105
KflI GGGWCCC 1 cut(s) 247
KroI GCCGGC 2 cut(s) 662, 1232
KroNI GCCGGC 2 cut(s) 664, 1234
Kzo9I GATC 6 cut(s) 220, 379, 412, 529, 542, 715
LmnI GCTCC 4 cut(s) 83, 276, 333, 999
Lsp1109I GCAGC 4 cut(s) 295, 479, 947, 1064
LweI GCATC 6 cut(s) 49, 271, 1011, 1095, 1116, 1171
MaeI CTAG 5 cut(s) 153, 239, 473, 912, 1038
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 3 cut(s) 503, 1207, 1253
MalI GATC 6 cut(s) 222, 381, 414, 531, 544, 717
MboI GATC 6 cut(s) 220, 379, 412, 529, 542, 715
MboII GAAGA 3 cut(s) 110, 365, 1268
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 2 cut(s) 598, 758
MluCI AATT 2 cut(s) 369, 1033
MlyI GAGTC 4 cut(s) 204, 264, 564, 981
MmeI TCCRAC 1 cut(s) 173
Mph1103I ATGCAT 1 cut(s) 1026
MroNI GCCGGC 2 cut(s) 662, 1232
MseI TTAA 3 cut(s) 69, 372, 1248
MslI CAYNNNNRTG 3 cut(s) 46, 761, 936
MspA1I CMGCKG 3 cut(s) 25, 535, 704
MspI CCGG 2 cut(s) 663, 1233
MspR9I CCNGG 6 cut(s) 102, 207, 278, 574, 868, 1271
Mva1269I GAATGC 2 cut(s) 186, 953
MvaI CCWGG 6 cut(s) 102, 207, 278, 574, 868, 1271
MwoI GCNNNNNNNGC 8 cut(s) 268, 273, 282, 613, 957, 1049, 1233, 1242
NaeI GCCGGC 2 cut(s) 664, 1234
NdeII GATC 6 cut(s) 220, 379, 412, 529, 542, 715
NgoMIV GCCGGC 2 cut(s) 662, 1232
NlaIII CATG 3 cut(s) 45, 407, 1105
NlaIV GGNNCC 7 cut(s) 248, 249, 296, 338, 590, 768, 1241
NmeAIII GCCGAG 1 cut(s) 1183
NmuCI GTSAC 2 cut(s) 503, 1253
NsiI ATGCAT 1 cut(s) 1026
NspI RCATGY 2 cut(s) 45, 407
NspV TTCGAA 1 cut(s) 1127
PaeI GCATGC 2 cut(s) 45, 407
PasI CCCWGGG 1 cut(s) 101
PctI GAATGC 2 cut(s) 186, 953
PdiI GCCGGC 2 cut(s) 664, 1234
PfeI GAWTC 2 cut(s) 992, 1213
PflMI CCANNNNNTGG 1 cut(s) 585
PfoI TCCNGGA 1 cut(s) 205
PkrI GCNGC 7 cut(s) 185, 310, 469, 618, 962, 1054, 1244
PleI GAGTC 4 cut(s) 204, 263, 563, 980
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
PpsI GAGTC 4 cut(s) 204, 263, 563, 980
Ppu21I YACGTR 1 cut(s) 170
PpuMI RGGWCCY 1 cut(s) 247
PsiI TTATAA 2 cut(s) 567, 641
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 6 cut(s) 100, 205, 276, 572, 866, 1269
PspCI CACGTG 1 cut(s) 170
PspGI CCWGG 6 cut(s) 100, 205, 276, 572, 866, 1269
PspN4I GGNNCC 7 cut(s) 248, 249, 296, 338, 590, 768, 1241
PspPI GGNCC 4 cut(s) 247, 576, 735, 1240
PspPPI RGGWCCY 1 cut(s) 247
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 1 cut(s) 529
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
RseI CAYNNNNRTG 3 cut(s) 46, 761, 936
SaqAI TTAA 3 cut(s) 69, 372, 1248
SatI GCNGC 7 cut(s) 184, 309, 468, 617, 961, 1053, 1243
Sau3AI GATC 6 cut(s) 220, 379, 412, 529, 542, 715
Sau96I GGNCC 4 cut(s) 247, 576, 735, 1240
SchI GAGTC 4 cut(s) 204, 264, 564, 981
ScrFI CCNGG 6 cut(s) 102, 207, 278, 574, 868, 1271
SduI GDGCHC 2 cut(s) 598, 758
SfaNI GCATC 6 cut(s) 49, 271, 1011, 1095, 1116, 1171
SfcI CTRYAG 2 cut(s) 465, 1176
SfuI TTCGAA 1 cut(s) 1127
SinI GGWCC 3 cut(s) 247, 576, 735
SmiMI CAYNNNNRTG 3 cut(s) 46, 761, 936
SphI GCATGC 2 cut(s) 45, 407
Sse9I AATT 2 cut(s) 369, 1033
SsiI CCGC 7 cut(s) 23, 184, 274, 533, 616, 666, 1243
SspI AATATT 1 cut(s) 461
SspMI CTAG 5 cut(s) 153, 239, 473, 912, 1038
StyD4I CCNGG 6 cut(s) 100, 205, 276, 572, 866, 1269
StyI CCWWGG 3 cut(s) 340, 472, 1203
TaaI ACNGT 3 cut(s) 593, 1016, 1076
TaiI ACGT 1 cut(s) 172
TaqI TCGA 4 cut(s) 258, 382, 826, 1127
TaqII GACCGA 1 cut(s) 678
TasI AATT 2 cut(s) 369, 1033
TatI WGTACW 1 cut(s) 490
TauI GCSGC 3 cut(s) 186, 619, 1245
TfiI GAWTC 2 cut(s) 992, 1213
Tru1I TTAA 3 cut(s) 69, 372, 1248
Tru9I TTAA 3 cut(s) 69, 372, 1248
TscAI CASTG 2 cut(s) 1141, 1271
TseFI GTSAC 2 cut(s) 503, 1253
TseI GCWGC 4 cut(s) 308, 467, 960, 1052
Tsp45I GTSAC 2 cut(s) 503, 1253
TspDTI ATGAA 3 cut(s) 134, 730, 763
TspGWI ACGGA 4 cut(s) 287, 320, 900, 1004
TspRI CASTG 2 cut(s) 1141, 1271
Van91I CCANNNNNTGG 1 cut(s) 585
VpaK11BI GGWCC 3 cut(s) 247, 576, 735
XceI RCATGY 2 cut(s) 45, 407
XmaJI CCTAGG 1 cut(s) 472
XmiI GTMKAC 1 cut(s) 846
XspI CTAG 5 cut(s) 153, 239, 473, 912, 1038
Zsp2I ATGCAT 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.