FvH4_3g45210

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002422
Physical Location & Seq
Forward (+)
37573486 .. 37578411
4926 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45210.t2

Sequence Viewer

Length: 936 bp
ATGGATTCGGTCCCTTCCAACTCCCATGGCAACCTCGACGAGCAGATTGCTCAGCTCATGCAGTGCAAGCCTTTGTCCGAACAAGAGGTAAGGGGGCTCTGTGAGAAGGCTAAGGAGATATTGATGGAAGAAAGCAATGTTCAGCCTGTGAAAAGCCCAGTGACTATTTGTGGTGATATTCACGGACAATTTCATGATCTTGCCGAGCTTTTCCGTATTGGAGGAAAGTGTCCAGATACCAATTATTTGTTTATGGGAGATTATGTGGATCGTGGCTATTACTCTGTCGAAACCGTAACACTTTTGGTGGCCTTGAAGGTTCGTTACTCTCAACGTATAACTATCCTTAGAGGGAATCATGAAAGTCGTCAGATTACTCAAGTTTATGGTTTTTATGACGAGTGCTTACGGAAGTATGGGAATGCCAATGTTTGGAAGACCTTCACAGATTTATTTGACTATTTTCCGTTGACAGCCTTGGTTGAATCTGAAATCTTCTGCTTGCATGGTGGACTGTCTCCATCGATTGAGACTCTAGATAACATACGTAATTTTGACCGTGTTCAAGAGGTTCCTCATGAAGGTCCCATGTGTGATCTTCTTTGGTCTGACCCGGATGATCGCTGTGGCTGGGGAATCTCTCCCAGAGGTGCTGGATATACTTTTGGCCAGGACATATCTGAGCAGTTCAATCATACCAATAACTTAAAGTTGATTGCTAGAGCACACCAGCTGGTCATGGAAGGTTACAACTGGGGTCATGAACAAAAAGTTGTCACCATATTCAGTGCACCCAACTACTGCTATCGCTGTGGAAATATGGCATCCATCCTGGAAGTCGATGACTGCAAAGGTCACACATTCATTCAGTTTGAGCCAGCTCCAAGGAGGGGAGAGCCAGACGTTACCCGAAGAACACCTGATTATTTCCTATAA

Protein Analysis

312

Amino Acids

35.51

Weight (kDa)

5.14

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 2 - 162 3.2e-36 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 432
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 667
AfiI CCNNNNNNNGG 3 cut(s) 432, 581, 890
AgsI TTSAA 4 cut(s) 316, 485, 566, 691
AjnI CCWGG 2 cut(s) 669, 831
AluBI AGCT 4 cut(s) 55, 208, 733, 881
AluI AGCT 4 cut(s) 55, 208, 733, 881
Alw21I GWGCWC 2 cut(s) 727, 793
Alw26I GTCTC 2 cut(s) 522, 524
Alw44I GTGCAC 1 cut(s) 789
AlwI GGATC 1 cut(s) 276
AoxI GGCC 2 cut(s) 309, 667
ApaLI GTGCAC 1 cut(s) 789
Asp700I GAANNNNTTC 1 cut(s) 440
AspS9I GGNCC 2 cut(s) 10, 584
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 185, 769
AvaII GGWCC 2 cut(s) 10, 584
BaeGI GKGCMC 1 cut(s) 793
BalI TGGCCA 1 cut(s) 669
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 308, 340
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 2 cut(s) 727, 793
BccI CCATC 3 cut(s) 118, 529, 836
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 2 cut(s) 671, 833
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 2 cut(s) 522, 524
BfaI CTAG 2 cut(s) 536, 720
BlpI GCTNAGC 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 614, 671, 833
Bme18I GGWCC 2 cut(s) 10, 584
BmgT120I GGNCC 2 cut(s) 10, 584
BmiI GGNNCC 3 cut(s) 12, 573, 586
BmrFI CCNGG 3 cut(s) 614, 671, 833
BmrI ACTGGG 2 cut(s) 152, 763
BmsI GCATC 1 cut(s) 833
BmuI ACTGGG 2 cut(s) 152, 763
BpiI GAAGAC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 3 cut(s) 25, 477, 884
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 3 cut(s) 432, 581, 890
Bse1I ACTGG 2 cut(s) 158, 758
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 2 cut(s) 671, 833
BseCI ATCGAT 1 cut(s) 524
BseDI CCNNGG 3 cut(s) 25, 477, 884
BseGI GGATG 3 cut(s) 622, 824, 828
BseLI CCNNNNNNNGG 3 cut(s) 432, 581, 890
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 65, 672
BseNI ACTGG 2 cut(s) 158, 758
BseSI GKGCMC 1 cut(s) 793
BseYI CCCAGC 1 cut(s) 630
BshFI GGCC 2 cut(s) 311, 669
BshVI ATCGAT 1 cut(s) 524
BsiHKAI GWGCWC 2 cut(s) 727, 793
BsiSI CCGG 1 cut(s) 614
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 3 cut(s) 432, 581, 890
BsmAI GTCTC 2 cut(s) 522, 524
BsmFI GGGAC 1 cut(s) 570
BsmI GAATGC 1 cut(s) 427
BsnI GGCC 2 cut(s) 311, 669
Bsp1286I GDGCHC 3 cut(s) 99, 727, 793
Bsp143I GATC 4 cut(s) 196, 268, 595, 619
Bsp1720I GCTNAGC 1 cut(s) 51
Bsp19I CCATGG 1 cut(s) 25
BspANI GGCC 2 cut(s) 311, 669
BspCNI CTCAG 2 cut(s) 64, 673
BspDI ATCGAT 1 cut(s) 524
BspHI TCATGA 4 cut(s) 193, 358, 577, 760
BspLI GGNNCC 3 cut(s) 12, 573, 586
BspPI GGATC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 142
BsrI ACTGG 2 cut(s) 158, 758
BssECI CCNNGG 3 cut(s) 25, 477, 884
BssMI GATC 4 cut(s) 196, 268, 595, 619
BssT1I CCWWGG 3 cut(s) 25, 477, 884
Bst2UI CCWGG 2 cut(s) 671, 833
Bst4CI ACNGT 3 cut(s) 295, 516, 560
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 3 cut(s) 68, 503, 879
BstDEI CTNAG 4 cut(s) 51, 111, 347, 681
BstDSI CCRYGG 1 cut(s) 25
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 3 cut(s) 622, 824, 828
BstKTI GATC 4 cut(s) 199, 271, 598, 622
BstMAI GTCTC 2 cut(s) 522, 524
BstMBI GATC 4 cut(s) 196, 268, 595, 619
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNI CCWGG 2 cut(s) 671, 833
BstSCI CCNGG 3 cut(s) 612, 669, 831
BstSLI GKGCMC 1 cut(s) 793
BstSNI TACGTA 1 cut(s) 548
BstV2I GAAGAC 1 cut(s) 443
Bsu15I ATCGAT 1 cut(s) 524
BsuRI GGCC 2 cut(s) 311, 669
BsuTUI ATCGAT 1 cut(s) 524
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 3 cut(s) 622, 824, 828
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 3 cut(s) 68, 165, 793
Cac8I GCNNGC 3 cut(s) 68, 503, 879
CciI TCATGA 4 cut(s) 193, 358, 577, 760
Cfr13I GGNCC 2 cut(s) 10, 584
ClaI ATCGAT 1 cut(s) 524
CviAII CATG 9 cut(s) 26, 58, 194, 359, 506, 578, 589, 739, 761
DdeI CTNAG 4 cut(s) 51, 111, 347, 681
DpnI GATC 4 cut(s) 198, 270, 597, 621
DpnII GATC 4 cut(s) 196, 268, 595, 619
EaeI YGGCCR 1 cut(s) 667
Eco105I TACGTA 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 25, 477, 884
Eco24I GRGCYC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 10, 584
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 2 cut(s) 669, 831
EcoT14I CCWWGG 3 cut(s) 25, 477, 884
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 3 cut(s) 25, 477, 884
FaeI CATG 9 cut(s) 29, 61, 197, 362, 509, 581, 592, 742, 764
FaqI GGGAC 1 cut(s) 570
FatI CATG 9 cut(s) 25, 57, 193, 358, 505, 577, 588, 738, 760
FokI GGATG 3 cut(s) 629, 811, 815
FriOI GRGCYC 1 cut(s) 99
FspBI CTAG 2 cut(s) 536, 720
GsaI CCCAGC 1 cut(s) 634
HaeIII GGCC 2 cut(s) 311, 669
HapII CCGG 1 cut(s) 614
Hin1II CATG 9 cut(s) 29, 61, 197, 362, 509, 581, 592, 742, 764
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HinfI GANTC 5 cut(s) 5, 355, 485, 532, 636
HpaII CCGG 1 cut(s) 614
HphI GGTGA 2 cut(s) 185, 769
Hpy166II GTNNAC 3 cut(s) 471, 512, 791
Hpy188I TCNGA 5 cut(s) 79, 372, 490, 610, 682
Hpy188III TCNNGA 7 cut(s) 194, 233, 359, 536, 566, 578, 761
Hpy8I GTNNAC 3 cut(s) 471, 512, 791
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 6 cut(s) 24, 100, 310, 451, 575, 737
HpyCH4III ACNGT 3 cut(s) 295, 516, 560
HpyCH4IV ACGT 3 cut(s) 334, 547, 903
HpyCH4V TGCA 5 cut(s) 61, 66, 505, 791, 849
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 4 cut(s) 51, 111, 347, 681
HpySE526I ACGT 3 cut(s) 334, 547, 903
Hsp92II CATG 9 cut(s) 29, 61, 197, 362, 509, 581, 592, 742, 764
Kzo9I GATC 4 cut(s) 196, 268, 595, 619
LmnI GCTCC 1 cut(s) 886
LweI GCATC 1 cut(s) 833
MaeI CTAG 2 cut(s) 536, 720
MaeII ACGT 3 cut(s) 334, 547, 903
MaeIII GTNAC 7 cut(s) 160, 295, 323, 746, 775, 854, 904
MalI GATC 4 cut(s) 198, 270, 597, 621
MboI GATC 4 cut(s) 196, 268, 595, 619
MboII GAAGA 5 cut(s) 140, 448, 487, 590, 924
MhlI GDGCHC 3 cut(s) 99, 727, 793
MlsI TGGCCA 1 cut(s) 669
MluCI AATT 3 cut(s) 188, 241, 550
MluNI TGGCCA 1 cut(s) 669
MlyI GAGTC 1 cut(s) 526
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 8 cut(s) 44, 79, 215, 344, 562, 585, 641, 882
Mox20I TGGCCA 1 cut(s) 669
MroXI GAANNNNTTC 1 cut(s) 440
MscI TGGCCA 1 cut(s) 669
MseI TTAA 1 cut(s) 707
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 614, 671, 833
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 2 cut(s) 671, 833
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 614
NcoI CCATGG 1 cut(s) 25
NdeII GATC 4 cut(s) 196, 268, 595, 619
NlaIII CATG 9 cut(s) 29, 61, 197, 362, 509, 581, 592, 742, 764
NlaIV GGNNCC 3 cut(s) 12, 573, 586
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 3 cut(s) 160, 775, 854
PagI TCATGA 4 cut(s) 193, 358, 577, 760
PctI GAATGC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 4 cut(s) 5, 355, 485, 636
PflMI CCANNNNNTGG 1 cut(s) 432
PfoI TCCNGGA 1 cut(s) 831
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 584
Psp6I CCWGG 2 cut(s) 669, 831
PspFI CCCAGC 1 cut(s) 630
PspGI CCWGG 2 cut(s) 669, 831
PspN4I GGNNCC 3 cut(s) 12, 573, 586
PspPI GGNCC 2 cut(s) 10, 584
PspPPI RGGWCCY 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 733
SaqAI TTAA 1 cut(s) 707
Sau3AI GATC 4 cut(s) 196, 268, 595, 619
Sau96I GGNCC 2 cut(s) 10, 584
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 3 cut(s) 614, 671, 833
SduI GDGCHC 3 cut(s) 99, 727, 793
SfaNI GCATC 1 cut(s) 833
SinI GGWCC 2 cut(s) 10, 584
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 3 cut(s) 188, 241, 550
SspMI CTAG 2 cut(s) 536, 720
StyD4I CCNGG 3 cut(s) 612, 669, 831
StyI CCWWGG 3 cut(s) 25, 477, 884
TaaI ACNGT 3 cut(s) 295, 516, 560
TaiI ACGT 3 cut(s) 337, 550, 906
TaqI TCGA 4 cut(s) 36, 288, 524, 840
TasI AATT 3 cut(s) 188, 241, 550
TfiI GAWTC 4 cut(s) 5, 355, 485, 636
Tru1I TTAA 1 cut(s) 707
Tru9I TTAA 1 cut(s) 707
TscAI CASTG 3 cut(s) 68, 165, 793
TseFI GTSAC 3 cut(s) 160, 775, 854
Tsp45I GTSAC 3 cut(s) 160, 775, 854
TspDTI ATGAA 5 cut(s) 182, 375, 594, 777, 853
TspGWI ACGGA 4 cut(s) 198, 203, 424, 456
TspRI CASTG 3 cut(s) 68, 165, 793
Van91I CCANNNNNTGG 1 cut(s) 432
VneI GTGCAC 1 cut(s) 789
VpaK11BI GGWCC 2 cut(s) 10, 584
XagI CCTNNNNNAGG 1 cut(s) 579
XbaI TCTAGA 1 cut(s) 535
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 2 cut(s) 536, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.