Rw5G049820

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002422
Physical Location & Seq
Reverse (-)
86354047 .. 86355134
1088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049820.1

Sequence Viewer

Length: 549 bp
ATGCCTGTTGATGCTAAATTTGAAATCATACATCTTAGTAGGATTGCTTTACAGCTAGAGTTTCACCTGTCATTTTGGCAACTCTGTTGGGTCGTTGAATCTGAAATCTTCTGCTTGCACGGTGGACTTTCTCCCTCAATTGAAACCCTTGATAACATACGTAATTTTGACCGTGTTCAAGAGGTTCCTCATGAAGGTCCCATGTGTGATCTTCTTTGGTCTGACCCGGATGATCGCTGTGGCTGGGGTATCTCTCCCAGAGGTGCTGGATATACTTTTGGCCAGGACATATCCGAGCAGTTCAATCATACCAATAACTTGAAGTTGATTGCTAGAGCACACCAGCTGGTCATGGAAGGTTATAACTGGGGTCATGAACAAAAAGTTGTCACCATATTCAGTGCACCCAACTACTGTTACCGCTGTGGAAATATGGCATCAATCCTAGAAGTCGATGACTGCAAAGGTCACACATTCATTCAGTTTGAGCCAGCTCCAAGGAGGGGAGAGCCAGATGTTACCCGAAGAACACCTGATTATTTCCTATAA

Protein Analysis

182

Amino Acids

20.96

Weight (kDa)

5.15

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 22 - 117 1.9e-16 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
AciI CCGC 1 cut(s) 421
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 194, 503
AgsI TTSAA 6 cut(s) 23, 98, 143, 179, 304, 322
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 3 cut(s) 55, 346, 494
AluI AGCT 3 cut(s) 55, 346, 494
Alw21I GWGCWC 2 cut(s) 340, 406
Alw44I GTGCAC 1 cut(s) 402
AoxI GGCC 1 cut(s) 280
ApaLI GTGCAC 1 cut(s) 402
ApoI RAATTY 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 197
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 56, 382
AvaII GGWCC 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 406
BalI TGGCCA 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 340, 406
BciT130I CCWGG 1 cut(s) 284
BcnI CCSGG 1 cut(s) 227
BfaI CTAG 3 cut(s) 56, 333, 446
Bme1390I CCNGG 2 cut(s) 227, 284
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 186, 199
BmrFI CCNGG 2 cut(s) 227, 284
BmrI ACTGGG 1 cut(s) 376
BmsI GCATC 1 cut(s) 446
BmuI ACTGGG 1 cut(s) 376
BpuMI CCSGG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 497
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 503
Bse1I ACTGG 1 cut(s) 371
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 2 cut(s) 194, 503
BseNI ACTGG 1 cut(s) 371
BseSI GKGCMC 1 cut(s) 406
BseYI CCCAGC 1 cut(s) 243
BshFI GGCC 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 340, 406
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 2 cut(s) 194, 503
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 340, 406
Bsp143I GATC 2 cut(s) 208, 232
BspACI CCGC 1 cut(s) 421
BspANI GGCC 1 cut(s) 282
BspHI TCATGA 2 cut(s) 190, 373
BspLI GGNNCC 2 cut(s) 186, 199
BsrI ACTGG 1 cut(s) 371
BssECI CCNNGG 1 cut(s) 497
BssMI GATC 2 cut(s) 208, 232
BssT1I CCWWGG 1 cut(s) 497
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 3 cut(s) 122, 173, 416
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 116, 492
BstDEI CTNAG 1 cut(s) 35
BstENI CCTNNNNNAGG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 2 cut(s) 211, 235
BstMBI GATC 2 cut(s) 208, 232
BstNI CCWGG 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 225, 282
BstSLI GKGCMC 1 cut(s) 406
BstSNI TACGTA 1 cut(s) 161
BsuRI GGCC 1 cut(s) 282
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 116, 492
CciI TCATGA 2 cut(s) 190, 373
Cfr13I GGNCC 1 cut(s) 197
CviAII CATG 4 cut(s) 191, 202, 352, 374
CviJI RGCY 7 cut(s) 55, 243, 282, 346, 490, 494, 511
CviKI_1 RGCY 7 cut(s) 55, 243, 282, 346, 490, 494, 511
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 210, 234
DpnII GATC 2 cut(s) 208, 232
EaeI YGGCCR 1 cut(s) 280
Eco105I TACGTA 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 197
EcoNI CCTNNNNNAGG 1 cut(s) 192
EcoO109I RGGNCCY 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 497
ErhI CCWWGG 1 cut(s) 497
FaeI CATG 4 cut(s) 194, 205, 355, 377
FaqI GGGAC 1 cut(s) 183
FatI CATG 4 cut(s) 190, 201, 351, 373
FokI GGATG 1 cut(s) 242
FspBI CTAG 3 cut(s) 56, 333, 446
GsaI CCCAGC 1 cut(s) 247
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 227
Hin1II CATG 4 cut(s) 194, 205, 355, 377
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 227
HphI GGTGA 2 cut(s) 56, 382
Hpy166II GTNNAC 2 cut(s) 125, 404
Hpy188I TCNGA 3 cut(s) 103, 223, 295
Hpy188III TCNNGA 3 cut(s) 179, 191, 374
Hpy8I GTNNAC 2 cut(s) 125, 404
HpyAV CCTTC 2 cut(s) 188, 350
HpyCH4III ACNGT 3 cut(s) 122, 173, 416
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 118, 404, 462
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 4 cut(s) 194, 205, 355, 377
Kzo9I GATC 2 cut(s) 208, 232
LmnI GCTCC 1 cut(s) 499
LweI GCATC 1 cut(s) 446
MaeI CTAG 3 cut(s) 56, 333, 446
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 4 cut(s) 388, 416, 467, 517
MalI GATC 2 cut(s) 210, 234
MboI GATC 2 cut(s) 208, 232
MboII GAAGA 3 cut(s) 100, 203, 537
MfeI CAATTG 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 340, 406
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 3 cut(s) 17, 138, 163
MluNI TGGCCA 1 cut(s) 282
MnlI CCTC 5 cut(s) 145, 175, 198, 254, 495
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 2 cut(s) 346, 423
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 227, 284
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 284
NciI CCSGG 1 cut(s) 227
NdeII GATC 2 cut(s) 208, 232
NlaIII CATG 4 cut(s) 194, 205, 355, 377
NlaIV GGNNCC 2 cut(s) 186, 199
NmuCI GTSAC 2 cut(s) 388, 467
PagI TCATGA 2 cut(s) 190, 373
PfeI GAWTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 197
PsiI TTATAA 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 2 cut(s) 186, 199
PspPI GGNCC 1 cut(s) 197
PspPPI RGGWCCY 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 346
Sau3AI GATC 2 cut(s) 208, 232
Sau96I GGNCC 1 cut(s) 197
ScrFI CCNGG 2 cut(s) 227, 284
SduI GDGCHC 2 cut(s) 340, 406
SfaNI GCATC 1 cut(s) 446
SinI GGWCC 1 cut(s) 197
SnaBI TACGTA 1 cut(s) 161
Sse9I AATT 3 cut(s) 17, 138, 163
SsiI CCGC 1 cut(s) 421
SspMI CTAG 3 cut(s) 56, 333, 446
StyD4I CCNGG 2 cut(s) 225, 282
StyI CCWWGG 1 cut(s) 497
TaaI ACNGT 3 cut(s) 122, 173, 416
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 453
TasI AATT 3 cut(s) 17, 138, 163
TfiI GAWTC 1 cut(s) 98
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 2 cut(s) 388, 467
Tsp45I GTSAC 2 cut(s) 388, 467
TspDTI ATGAA 3 cut(s) 207, 390, 466
TspRI CASTG 1 cut(s) 406
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 197
XagI CCTNNNNNAGG 1 cut(s) 192
XapI RAATTY 1 cut(s) 17
XspI CTAG 3 cut(s) 56, 333, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.