FvH4_6g21560

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002422
Physical Location & Seq
Reverse (-)
15130287 .. 15136052
5766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21560.t2

Sequence Viewer

Length: 852 bp
ATGGGCGTCAATTTGGCTTCCTCCGATTCCAGCAATGATCTCAACGAGCAGATCGAGCAGCTCATGCAGTGCAAGCCACTCTCCGAGCAACAGGTGAGAGCGCTATGCGAGAAGGCAAAGGAAATCTTAATGGAAGAAAGCAATGTCCAGCCGGTAAAAAGCCCTGTGACAATTTGTGGCGATATTCATGGGCAGTTTCACGACCTTGCAGAGCTTTTTCGAATTGGAGGGAAGTGTCCCGACACCAATTACTTATTTATGGGAGATTATGTGGACCGTGGTTATTACTCAGTCGAAACTGTGACGCTCCTAGTGGCCCTGAAAGTGCGTTATCATCAGCGTATCACTATTCTTAGAGGAAACCACGAAAGCCGTCAGATTACTCAGGTTTATGGGTTTTACGATGAATGTCTTCGCAAGTATGGGAATGCCAGTGTATGGAAGACCTTCACCGACCTTTTTGACTACTTTCCACTGACAGCATTGGTTCCACACGAAGGGCCCATGTGTGATCTGTTATGGTCTGACCCAGATGACAGATGTGGTTGGGGTATTTCACCTCGTGGTGCAGGATATACTTTTGGCCAGGATATATCTGAACAATTCAATCATACAAATAGTTTAAAGTTGATTGCAAGAGCTCATCAACTGGTTATGGATGGATTCAACTGGGCCCATGAACAAAAGGTGGTTACCATATTCAGTGCACCCAATTATTGCTATCGCTGTGGGAACATGGCATCTGTTTTAGAAGTCGATGACTGCAGGGGCCATACTTTCATCCAGTTTGAGCCTGCTCCTAGGAGAGGAGAACCAGATGTTACTCGTAGAACACCTGATTACTTTTTGTGA

Protein Analysis

284

Amino Acids

32.33

Weight (kDa)

5.36

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 55 - 248 1.9e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 438
AcoI YGGCCR 1 cut(s) 583
AcyI GRCGYC 1 cut(s) 6
AdeI CACNNNGTG 1 cut(s) 563
AfeI AGCGCT 1 cut(s) 102
AfiI CCNNNNNNNGG 3 cut(s) 438, 497, 806
AgsI TTSAA 2 cut(s) 607, 667
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 3 cut(s) 61, 214, 641
AluI AGCT 3 cut(s) 61, 214, 641
Alw21I GWGCWC 2 cut(s) 643, 709
Alw44I GTGCAC 1 cut(s) 705
Aor51HI AGCGCT 1 cut(s) 102
AoxI GGCC 5 cut(s) 315, 500, 583, 672, 769
ApaI GGGCCC 2 cut(s) 504, 676
ApaLI GTGCAC 1 cut(s) 705
ApeKI GCWGC 1 cut(s) 58
Asp700I GAANNNNTTC 2 cut(s) 411, 446
AspA2I CCTAGG 1 cut(s) 800
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 7 cut(s) 274, 316, 500, 501, 672, 673, 769
AsuHPI GGTGA 3 cut(s) 106, 442, 549
AsuII TTCGAA 1 cut(s) 220
AvaII GGWCC 1 cut(s) 274
AvrII CCTAGG 1 cut(s) 800
BaeGI GKGCMC 3 cut(s) 504, 676, 709
BalI TGGCCA 1 cut(s) 585
BanII GRGCYC 3 cut(s) 504, 643, 676
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 2 cut(s) 404, 449
Bbv12I GWGCWC 2 cut(s) 643, 709
BbvI GCAGC 1 cut(s) 70
BccI CCATC 1 cut(s) 653
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 1 cut(s) 587
BfaI CTAG 2 cut(s) 311, 801
BfmI CTRYAG 1 cut(s) 763
BfoI RGCGCY 1 cut(s) 104
BisI GCNGC 1 cut(s) 59
BlnI CCTAGG 1 cut(s) 800
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 587
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 7 cut(s) 274, 316, 500, 501, 672, 673, 769
BmiI GGNNCC 4 cut(s) 489, 502, 674, 770
BmrFI CCNGG 1 cut(s) 587
BmrI ACTGGG 1 cut(s) 679
BmsI GCATC 1 cut(s) 749
BmuI ACTGGG 1 cut(s) 679
BpiI GAAGAC 2 cut(s) 404, 449
Bpu14I TTCGAA 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 277, 800
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 3 cut(s) 438, 497, 806
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 4 cut(s) 432, 654, 674, 784
Bse3DI GCAATG 2 cut(s) 40, 148
BseBI CCWGG 1 cut(s) 587
BseDI CCNNGG 2 cut(s) 277, 800
BseGI GGATG 2 cut(s) 664, 780
BseLI CCNNNNNNNGG 3 cut(s) 438, 497, 806
BseMI GCAATG 2 cut(s) 40, 148
BseMII CTCAG 2 cut(s) 303, 398
BseNI ACTGG 4 cut(s) 432, 654, 674, 784
BseRI GAGGAG 1 cut(s) 822
BseSI GKGCMC 3 cut(s) 504, 676, 709
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 588
BshFI GGCC 5 cut(s) 317, 502, 585, 674, 771
BsiHKAI GWGCWC 2 cut(s) 643, 709
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 438, 497, 806
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 5 cut(s) 317, 502, 585, 674, 771
Bsp119I TTCGAA 1 cut(s) 220
Bsp120I GGGCCC 2 cut(s) 500, 672
Bsp1286I GDGCHC 4 cut(s) 504, 643, 676, 709
Bsp143I GATC 3 cut(s) 37, 51, 511
BspANI GGCC 5 cut(s) 317, 502, 585, 674, 771
BspCNI CTCAG 2 cut(s) 302, 397
BspLI GGNNCC 4 cut(s) 489, 502, 674, 770
BspMAI CTGCAG 1 cut(s) 767
BspT104I TTCGAA 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 40, 148
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 4 cut(s) 432, 654, 674, 784
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 277, 800
BssMI GATC 3 cut(s) 37, 51, 511
BssNI GRCGYC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 1 cut(s) 800
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 2 cut(s) 278, 301
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 2 cut(s) 74, 795
BstDEI CTNAG 3 cut(s) 289, 353, 384
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 1 cut(s) 691
BstENI CCTNNNNNAGG 1 cut(s) 804
BstF5I GGATG 2 cut(s) 664, 780
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 3 cut(s) 40, 54, 514
BstMBI GATC 3 cut(s) 37, 51, 511
BstMWI GCNNNNNNNGC 3 cut(s) 55, 64, 73
BstNI CCWGG 1 cut(s) 587
BstPI GGTNACC 1 cut(s) 691
BstSCI CCNGG 1 cut(s) 585
BstSFI CTRYAG 1 cut(s) 763
BstSLI GKGCMC 3 cut(s) 504, 676, 709
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 2 cut(s) 404, 449
BsuRI GGCC 5 cut(s) 317, 502, 585, 674, 771
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 664, 780
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 4 cut(s) 74, 439, 473, 709
Cac8I GCNNGC 2 cut(s) 74, 795
CfoI GCGC 1 cut(s) 103
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 7 cut(s) 274, 316, 500, 501, 672, 673, 769
CseI GACGC 1 cut(s) 313
CviAII CATG 5 cut(s) 64, 188, 505, 677, 736
DdeI CTNAG 3 cut(s) 289, 353, 384
DpnI GATC 3 cut(s) 39, 53, 513
DpnII GATC 3 cut(s) 37, 51, 511
DraI TTTAAA 1 cut(s) 624
DraIII CACNNNGTG 1 cut(s) 563
EaeI YGGCCR 1 cut(s) 583
Ecl136II GAGCTC 1 cut(s) 641
Eco130I CCWWGG 1 cut(s) 800
Eco24I GRGCYC 3 cut(s) 504, 643, 676
Eco47I GGWCC 1 cut(s) 274
Eco47III AGCGCT 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 641
Eco91I GGTNACC 1 cut(s) 691
EcoICRI GAGCTC 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 804
EcoO109I RGGNCCY 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 585
EcoT14I CCWWGG 1 cut(s) 800
EcoT38I GRGCYC 3 cut(s) 504, 643, 676
ErhI CCWWGG 1 cut(s) 800
FaeI CATG 5 cut(s) 67, 191, 508, 680, 739
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 222
FatI CATG 5 cut(s) 63, 187, 504, 676, 735
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 2 cut(s) 671, 767
FriOI GRGCYC 3 cut(s) 504, 643, 676
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 2 cut(s) 311, 801
GlaI GCGC 1 cut(s) 102
GluI GCNGC 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 5 cut(s) 317, 502, 585, 674, 771
HapII CCGG 1 cut(s) 152
HgaI GACGC 1 cut(s) 313
HhaI GCGC 1 cut(s) 103
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 5 cut(s) 67, 191, 508, 680, 739
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HinfI GANTC 2 cut(s) 26, 663
HpaII CCGG 1 cut(s) 152
HphI GGTGA 3 cut(s) 106, 442, 549
Hpy166II GTNNAC 2 cut(s) 274, 707
Hpy188I TCNGA 5 cut(s) 25, 85, 378, 526, 598
Hpy188III TCNNGA 2 cut(s) 200, 239
Hpy8I GTNNAC 2 cut(s) 274, 707
HpyAV CCTTC 3 cut(s) 106, 457, 491
HpyCH4III ACNGT 2 cut(s) 278, 301
HpyCH4V TGCA 7 cut(s) 67, 72, 209, 569, 635, 707, 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 64, 73
HpyF3I CTNAG 3 cut(s) 289, 353, 384
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 5 cut(s) 67, 191, 508, 680, 739
HspAI GCGC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 37, 51, 511
LmnI GCTCC 2 cut(s) 312, 802
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 749
MaeI CTAG 2 cut(s) 311, 801
MaeIII GTNAC 4 cut(s) 166, 301, 691, 820
MalI GATC 3 cut(s) 39, 53, 513
MboI GATC 3 cut(s) 37, 51, 511
MboII GAAGA 3 cut(s) 146, 404, 454
MhlI GDGCHC 4 cut(s) 504, 643, 676, 709
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 6 cut(s) 10, 171, 222, 247, 602, 712
MluNI TGGCCA 1 cut(s) 585
MnlI CCTC 5 cut(s) 31, 221, 350, 570, 800
Mox20I TGGCCA 1 cut(s) 585
MroXI GAANNNNTTC 2 cut(s) 411, 446
MscI TGGCCA 1 cut(s) 585
MseI TTAA 2 cut(s) 128, 623
Msp20I TGGCCA 1 cut(s) 585
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 587
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 1 cut(s) 587
MwoI GCNNNNNNNGC 3 cut(s) 55, 64, 73
NdeII GATC 3 cut(s) 37, 51, 511
NlaIII CATG 5 cut(s) 67, 191, 508, 680, 739
NlaIV GGNNCC 4 cut(s) 489, 502, 674, 770
NmuCI GTSAC 2 cut(s) 166, 301
NspV TTCGAA 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 433
PdmI GAANNNNTTC 2 cut(s) 411, 446
PfeI GAWTC 2 cut(s) 26, 663
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 585
PspEI GGTNACC 1 cut(s) 691
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 4 cut(s) 489, 502, 674, 770
PspOMI GGGCCC 2 cut(s) 500, 672
PspPI GGNCC 7 cut(s) 274, 316, 500, 501, 672, 673, 769
PstI CTGCAG 1 cut(s) 767
SacI GAGCTC 1 cut(s) 643
SaqAI TTAA 2 cut(s) 128, 623
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 3 cut(s) 37, 51, 511
Sau96I GGNCC 7 cut(s) 274, 316, 500, 501, 672, 673, 769
ScrFI CCNGG 1 cut(s) 587
SduI GDGCHC 4 cut(s) 504, 643, 676, 709
SfaNI GCATC 1 cut(s) 749
SfcI CTRYAG 1 cut(s) 763
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 1 cut(s) 274
Sse9I AATT 6 cut(s) 10, 171, 222, 247, 602, 712
SspMI CTAG 2 cut(s) 311, 801
SstI GAGCTC 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 585
StyI CCWWGG 1 cut(s) 800
TaaI ACNGT 2 cut(s) 278, 301
TaqI TCGA 4 cut(s) 54, 220, 294, 756
TasI AATT 6 cut(s) 10, 171, 222, 247, 602, 712
TfiI GAWTC 2 cut(s) 26, 663
Tru1I TTAA 2 cut(s) 128, 623
Tru9I TTAA 2 cut(s) 128, 623
TscAI CASTG 4 cut(s) 74, 439, 480, 709
TseFI GTSAC 2 cut(s) 166, 301
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 2 cut(s) 166, 301
TspDTI ATGAA 4 cut(s) 176, 420, 693, 769
TspRI CASTG 4 cut(s) 74, 439, 480, 709
Van91I CCANNNNNTGG 1 cut(s) 438
VneI GTGCAC 1 cut(s) 705
VpaK11BI GGWCC 1 cut(s) 274
XagI CCTNNNNNAGG 1 cut(s) 804
XmaJI CCTAGG 1 cut(s) 800
XmnI GAANNNNTTC 2 cut(s) 411, 446
XspI CTAG 2 cut(s) 311, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.