MD03G1007300.v1.1

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002422
Physical Location & Seq
Reverse (-)
580509 .. 585577
5069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1007300.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGATTCAGTCCCTTCGAACTCACATGGGAACCTGGATGAACAGATTGCTCAGCTCATGCAATGCAAGCCCTTGTCCGAACAAGAGGTAAGAGTGCTCTGCGAGAAGGCCAAGGAAATATTAATGGAAGAAAGCAATGTCCAGCCTGTAAAAAGCCCTGTAACTATCTGTGGGGATATTCATGGACAATTCCATGATCTGGCGGAGCTCTTCCGCATTGGAGGAAAGTGCCCAGATACAAATTATTTGTTTATGGGAGATTATGTGGACCGTGGCTATTATTCAGTTGAAACAGTGACACTCCTAGTGGCCTTGAAGGTGCGTTATCATCAGCGTATTACTATCCTAAGAGGAAACCATGAAAGCCGTCAGATTACTCAAGTTTATGGTTTTTATGACGAGTGCCTACGGAAGTATGGTAATGCCAATGTTTGGAAGATCTTTACAGATTTATTTGACTATTTTCCACTGACAGCATTGGTTGAAAGTGAAATATTCTGTCTGCATGGAGGGCTGTCTCCATCTATTGAAACCCTTGATAACATACGTAATTTTGACCGTGTACAAGAGGTTCCTCATGAGGGTCCCATGTGTGATCTTTTATGGTCTGACCCTGATGATCGCTGTGGTTGGGGTATCTCTCCCAGGGGTGCTGGATATACGTTTGGTCAGGACATATCCGAGCAGTTTAATCACACAAATAACTTAAAGTTGATTGCTAGAGCACATCAGCTGGTCATGGAAGGATACAACTGGGGTCATGAACAAAAAGTGGTCACTATATTTAGCGCACCCAACTATTGTTATCGCTGTGGAAATATGGCATCCATCCTGGAAGTTGATGACTGCAAAGGTCACACATTTATTCAGTTTGAGCCAGCTCCAAGGAGGGGAGAACCAGATGTGACCCGAAGAACACCTGATTACTTCCTATAA

Protein Analysis

312

Amino Acids

35.62

Weight (kDa)

5.21

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 53 - 246 6.1e-47 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 199, 432
AciI CCGC 2 cut(s) 203, 214
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
AgsI TTSAA 4 cut(s) 290, 316, 485, 530
AjnI CCWGG 3 cut(s) 33, 644, 831
AluBI AGCT 4 cut(s) 55, 208, 733, 881
AluI AGCT 4 cut(s) 55, 208, 733, 881
Alw21I GWGCWC 3 cut(s) 99, 210, 727
Alw26I GTCTC 1 cut(s) 522
AoxI GGCC 2 cut(s) 108, 309
AseI ATTAAT 1 cut(s) 122
AspLEI GCGC 1 cut(s) 791
AspS9I GGNCC 2 cut(s) 268, 584
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 2 cut(s) 268, 584
BaeGI GKGCMC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 3 cut(s) 99, 210, 727
BccI CCATC 2 cut(s) 529, 836
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 3 cut(s) 35, 646, 833
BciVI GTATCC 1 cut(s) 740
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 2 cut(s) 305, 720
BfuI GTATCC 1 cut(s) 740
BglII AGATCT 1 cut(s) 438
BlpI GCTNAGC 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 35, 646, 833
Bme18I GGWCC 2 cut(s) 268, 584
BmgT120I GGNCC 2 cut(s) 268, 584
BmiI GGNNCC 4 cut(s) 32, 573, 585, 586
BmrFI CCNGG 3 cut(s) 35, 646, 833
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 763
Bpu1102I GCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 5 cut(s) 111, 271, 644, 645, 884
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 2 cut(s) 68, 142
BseBI CCWGG 3 cut(s) 35, 646, 833
BseDI CCNNGG 5 cut(s) 111, 271, 644, 645, 884
BseGI GGATG 3 cut(s) 43, 824, 828
BseLI CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
BseMI GCAATG 2 cut(s) 68, 142
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 1 cut(s) 758
BseSI GKGCMC 1 cut(s) 233
BshFI GGCC 2 cut(s) 110, 311
BsiHKAI GWGCWC 3 cut(s) 99, 210, 727
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 570
BsnI GGCC 2 cut(s) 110, 311
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 4 cut(s) 99, 210, 233, 727
Bsp1407I TGTACA 1 cut(s) 562
Bsp143I GATC 4 cut(s) 196, 438, 595, 619
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 2 cut(s) 203, 214
BspANI GGCC 2 cut(s) 110, 311
BspCNI CTCAG 1 cut(s) 64
BspHI TCATGA 2 cut(s) 577, 760
BspLI GGNNCC 4 cut(s) 32, 573, 585, 586
BspQI GCTCTTC 1 cut(s) 215
BspT104I TTCGAA 1 cut(s) 17
BsrDI GCAATG 2 cut(s) 68, 142
BsrGI TGTACA 1 cut(s) 562
BsrI ACTGG 1 cut(s) 758
BssECI CCNNGG 5 cut(s) 111, 271, 644, 645, 884
BssMI GATC 4 cut(s) 196, 438, 595, 619
BssT1I CCWWGG 2 cut(s) 111, 884
Bst2UI CCWGG 3 cut(s) 35, 646, 833
Bst4CI ACNGT 3 cut(s) 272, 295, 560
Bst6I CTCTTC 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 562
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 68, 879
BstDEI CTNAG 2 cut(s) 51, 347
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 3 cut(s) 43, 824, 828
BstHHI GCGC 1 cut(s) 791
BstKTI GATC 4 cut(s) 199, 441, 598, 622
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 4 cut(s) 196, 438, 595, 619
BstMWI GCNNNNNNNGC 2 cut(s) 67, 511
BstNI CCWGG 3 cut(s) 35, 646, 833
BstSCI CCNGG 3 cut(s) 33, 644, 831
BstSLI GKGCMC 1 cut(s) 233
BstSNI TACGTA 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 2 cut(s) 110, 311
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 3 cut(s) 43, 824, 828
BtsIMutI CAGTG 2 cut(s) 300, 467
Cac8I GCNNGC 2 cut(s) 68, 879
CciI TCATGA 2 cut(s) 577, 760
CfoI GCGC 1 cut(s) 791
Cfr13I GGNCC 2 cut(s) 268, 584
Csp6I GTAC 1 cut(s) 563
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 2 cut(s) 51, 347
DpnI GATC 4 cut(s) 198, 440, 597, 621
DpnII GATC 4 cut(s) 196, 438, 595, 619
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EciI GGCGGA 1 cut(s) 218
Ecl136II GAGCTC 1 cut(s) 208
Eco105I TACGTA 1 cut(s) 548
Eco130I CCWWGG 2 cut(s) 111, 884
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 2 cut(s) 268, 584
Eco53kI GAGCTC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 3 cut(s) 33, 644, 831
EcoT14I CCWWGG 2 cut(s) 111, 884
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 2 cut(s) 111, 884
FaqI GGGAC 1 cut(s) 570
FokI GGATG 3 cut(s) 50, 811, 815
FriOI GRGCYC 1 cut(s) 210
FspBI CTAG 2 cut(s) 305, 720
GlaI GCGC 1 cut(s) 790
HaeIII GGCC 2 cut(s) 110, 311
HhaI GCGC 1 cut(s) 791
Hin6I GCGC 1 cut(s) 789
HinP1I GCGC 1 cut(s) 789
HinfI GANTC 1 cut(s) 5
Hpy166II GTNNAC 2 cut(s) 268, 563
Hpy188I TCNGA 4 cut(s) 79, 372, 610, 682
Hpy188III TCNNGA 3 cut(s) 578, 671, 761
Hpy8I GTNNAC 2 cut(s) 268, 563
HpyAV CCTTC 4 cut(s) 24, 100, 310, 737
HpyCH4III ACNGT 3 cut(s) 272, 295, 560
HpyCH4IV ACGT 2 cut(s) 547, 662
HpyCH4V TGCA 4 cut(s) 61, 66, 505, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 511
HpyF3I CTNAG 2 cut(s) 51, 347
HpySE526I ACGT 2 cut(s) 547, 662
HspAI GCGC 1 cut(s) 789
KflI GGGWCCC 1 cut(s) 584
Kzo9I GATC 4 cut(s) 196, 438, 595, 619
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 2 cut(s) 205, 886
LweI GCATC 1 cut(s) 833
MaeI CTAG 2 cut(s) 305, 720
MaeII ACGT 2 cut(s) 547, 662
MaeIII GTNAC 5 cut(s) 160, 295, 775, 854, 904
MalI GATC 4 cut(s) 198, 440, 597, 621
MboI GATC 4 cut(s) 196, 438, 595, 619
MboII GAAGA 4 cut(s) 140, 202, 448, 924
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 4 cut(s) 99, 210, 233, 727
MluCI AATT 3 cut(s) 188, 241, 550
MnlI CCTC 8 cut(s) 79, 215, 344, 503, 562, 574, 585, 882
MseI TTAA 3 cut(s) 122, 690, 707
MspA1I CMGCKG 1 cut(s) 733
MspR9I CCNGG 3 cut(s) 35, 646, 833
MvaI CCWGG 3 cut(s) 35, 646, 833
MwoI GCNNNNNNNGC 2 cut(s) 67, 511
NdeII GATC 4 cut(s) 196, 438, 595, 619
NlaIV GGNNCC 4 cut(s) 32, 573, 585, 586
NmuCI GTSAC 4 cut(s) 295, 775, 854, 904
NspV TTCGAA 1 cut(s) 17
PagI TCATGA 2 cut(s) 577, 760
PasI CCCWGGG 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 5
PflMI CCANNNNNTGG 2 cut(s) 199, 432
PfoI TCCNGGA 1 cut(s) 831
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 584
PshBI ATTAAT 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 584
Psp6I CCWGG 3 cut(s) 33, 644, 831
PspGI CCWGG 3 cut(s) 33, 644, 831
PspN4I GGNNCC 4 cut(s) 32, 573, 585, 586
PspPI GGNCC 2 cut(s) 268, 584
PspPPI RGGWCCY 1 cut(s) 584
PsrI GAACNNNNNNTAC 2 cut(s) 555, 587
PsuI RGATCY 1 cut(s) 438
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 215
SaqAI TTAA 3 cut(s) 122, 690, 707
Sau3AI GATC 4 cut(s) 196, 438, 595, 619
Sau96I GGNCC 2 cut(s) 268, 584
ScrFI CCNGG 3 cut(s) 35, 646, 833
SduI GDGCHC 4 cut(s) 99, 210, 233, 727
SfaNI GCATC 1 cut(s) 833
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 2 cut(s) 268, 584
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 3 cut(s) 188, 241, 550
SsiI CCGC 2 cut(s) 203, 214
SspI AATATT 2 cut(s) 120, 495
SspMI CTAG 2 cut(s) 305, 720
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 33, 644, 831
StyI CCWWGG 2 cut(s) 111, 884
TaaI ACNGT 3 cut(s) 272, 295, 560
TaiI ACGT 2 cut(s) 550, 665
TaqI TCGA 1 cut(s) 17
TasI AATT 3 cut(s) 188, 241, 550
TatI WGTACW 1 cut(s) 562
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 3 cut(s) 122, 690, 707
Tru9I TTAA 3 cut(s) 122, 690, 707
TscAI CASTG 2 cut(s) 300, 474
TseFI GTSAC 4 cut(s) 295, 775, 854, 904
Tsp45I GTSAC 4 cut(s) 295, 775, 854, 904
TspDTI ATGAA 4 cut(s) 54, 170, 375, 777
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 2 cut(s) 300, 474
Van91I CCANNNNNTGG 2 cut(s) 199, 432
VpaK11BI GGWCC 2 cut(s) 268, 584
VspI ATTAAT 1 cut(s) 122
XspI CTAG 2 cut(s) 305, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.