MD11G1010600.v1.1

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002422
Physical Location & Seq
Reverse (-)
795712 .. 801172
5461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1010600.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGATTCGGTCCCTTCGAACTCCCATGGGAATCTGGATGAGCAGATTGCTCAACTCATGCAGTGCAAGCCCTTGTCCGAGCAAGAGGTAAGGGTGCTCTGCGAGAAGGCCAAGGAAATATTAATGGAAGAAAGCAATGTCCAGCCTGTAAAAAGCCCTGTAACTATCTGTGGTGATATTCATGGACAATTCCATGATCTGGCGGAGCTTTTCCGCATTGGAGGAAAGTGCCCAGATACAAACTATTTGTTTATGGGAGATTATGTGGACCGTGGCTATTATTCAGTTGAAACAGTAACTCTCCTTGTGGCCTTGAAGGTGCGTTATCATCAGCGTATTACTATCCTAAGAGGAAACCATGAAAGCCGTCAGATTACTCAAGTTTATGGTTTTTATGATGAGTGCCTACGGAAGTATGGTAATGCCAATGTTTGGAAGATCTTTACAGATTTATTTGACTATTTCCCGCTCACAGCATTGGTTGAATCTGAAATATTCTGCTTGCATGGAGGGCTGTCTCCATCCATTGAAACCCTCGATAACATACGTAATTTTGACCGTGTACAAGAGGTTCCTCATGAGGGTCCCATGTGTGATCTTTTATGGTCTGACCCTGATGATCGCTGTGGTTGGGGTATCTCTCCGAGGGGTGCTGGATATACGTTTGGCCAGGACATATCTGAGCAGTTTAATCATACAAATAACTTAAAGTTGATTGCTAGAGCACATCAGCTGGTCATGGAAGGATACAACTGGGGTCATGAACAAAAAGTGGTCACTATATTTAGTGCACCCAATTATTGTTATCGCTGTGGAAATATGGCATCCATCTTGGAAGTCGATGACTGCAAAGGTCACACATTCATTCAGTTTGAGCCAGCTCCGAGGAGGGGAGAACCAGATGTGACCCGAAGAACACCTGATTACTTCCTATAA

Protein Analysis

312

Amino Acids

35.62

Weight (kDa)

5.21

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 53 - 246 6.1e-47 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 199, 432
AccBSI CCGCTC 1 cut(s) 469
AciI CCGC 3 cut(s) 203, 214, 467
AcoI YGGCCR 1 cut(s) 667
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
AgsI TTSAA 4 cut(s) 290, 316, 485, 530
AjnI CCWGG 1 cut(s) 669
AluBI AGCT 3 cut(s) 208, 733, 881
AluI AGCT 3 cut(s) 208, 733, 881
Alw21I GWGCWC 3 cut(s) 99, 727, 793
Alw26I GTCTC 1 cut(s) 522
Alw44I GTGCAC 1 cut(s) 789
AoxI GGCC 3 cut(s) 108, 309, 667
ApaLI GTGCAC 1 cut(s) 789
AseI ATTAAT 1 cut(s) 122
AspS9I GGNCC 3 cut(s) 10, 268, 584
AsuHPI GGTGA 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 3 cut(s) 10, 268, 584
BaeGI GKGCMC 2 cut(s) 233, 793
BalI TGGCCA 1 cut(s) 669
Bbv12I GWGCWC 3 cut(s) 99, 727, 793
BccI CCATC 2 cut(s) 529, 836
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 1 cut(s) 671
BciVI GTATCC 1 cut(s) 740
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 720
BfuI GTATCC 1 cut(s) 740
BglII AGATCT 1 cut(s) 438
Bme1390I CCNGG 1 cut(s) 671
Bme18I GGWCC 3 cut(s) 10, 268, 584
BmgT120I GGNCC 3 cut(s) 10, 268, 584
BmiI GGNNCC 4 cut(s) 12, 573, 585, 586
BmrFI CCNGG 1 cut(s) 671
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 763
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 5 cut(s) 25, 111, 271, 644, 884
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 671
BseDI CCNNGG 5 cut(s) 25, 111, 271, 644, 884
BseGI GGATG 3 cut(s) 43, 521, 824
BseLI CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 1 cut(s) 901
BseSI GKGCMC 2 cut(s) 233, 793
BshFI GGCC 3 cut(s) 110, 311, 669
BsiHKAI GWGCWC 3 cut(s) 99, 727, 793
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 4 cut(s) 199, 432, 581, 890
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 570
BsnI GGCC 3 cut(s) 110, 311, 669
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 4 cut(s) 99, 233, 727, 793
Bsp1407I TGTACA 1 cut(s) 562
Bsp143I GATC 4 cut(s) 196, 438, 595, 619
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 3 cut(s) 203, 214, 467
BspANI GGCC 3 cut(s) 110, 311, 669
BspCNI CTCAG 1 cut(s) 673
BspHI TCATGA 2 cut(s) 577, 760
BspLI GGNNCC 4 cut(s) 12, 573, 585, 586
BspT104I TTCGAA 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 142
BsrGI TGTACA 1 cut(s) 562
BsrI ACTGG 1 cut(s) 758
BssECI CCNNGG 5 cut(s) 25, 111, 271, 644, 884
BssMI GATC 4 cut(s) 196, 438, 595, 619
BssT1I CCWWGG 2 cut(s) 25, 111
Bst2UI CCWGG 1 cut(s) 671
Bst4CI ACNGT 3 cut(s) 272, 295, 560
BstAUI TGTACA 1 cut(s) 562
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 3 cut(s) 68, 503, 879
BstDEI CTNAG 2 cut(s) 347, 681
BstDSI CCRYGG 2 cut(s) 25, 271
BstF5I GGATG 3 cut(s) 43, 521, 824
BstKTI GATC 4 cut(s) 199, 441, 598, 622
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 4 cut(s) 196, 438, 595, 619
BstMWI GCNNNNNNNGC 2 cut(s) 67, 511
BstNI CCWGG 1 cut(s) 671
BstSCI CCNGG 1 cut(s) 669
BstSLI GKGCMC 2 cut(s) 233, 793
BstSNI TACGTA 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 3 cut(s) 110, 311, 669
BtgI CCRYGG 2 cut(s) 25, 271
BtsCI GGATG 3 cut(s) 43, 521, 824
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 68
Cac8I GCNNGC 3 cut(s) 68, 503, 879
CciI TCATGA 2 cut(s) 577, 760
Cfr13I GGNCC 3 cut(s) 10, 268, 584
Csp6I GTAC 1 cut(s) 563
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 2 cut(s) 347, 681
DpnI GATC 4 cut(s) 198, 440, 597, 621
DpnII GATC 4 cut(s) 196, 438, 595, 619
EaeI YGGCCR 1 cut(s) 667
EciI GGCGGA 1 cut(s) 218
Eco105I TACGTA 1 cut(s) 548
Eco130I CCWWGG 2 cut(s) 25, 111
Eco47I GGWCC 3 cut(s) 10, 268, 584
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 25, 111
ErhI CCWWGG 2 cut(s) 25, 111
FaqI GGGAC 1 cut(s) 570
FauI CCCGC 1 cut(s) 474
FokI GGATG 3 cut(s) 50, 508, 811
FspBI CTAG 1 cut(s) 720
HaeIII GGCC 3 cut(s) 110, 311, 669
HinfI GANTC 3 cut(s) 5, 31, 485
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 3 cut(s) 268, 563, 791
Hpy188I TCNGA 7 cut(s) 79, 372, 490, 610, 645, 682, 885
Hpy188III TCNNGA 3 cut(s) 35, 578, 761
Hpy8I GTNNAC 3 cut(s) 268, 563, 791
HpyAV CCTTC 4 cut(s) 24, 100, 310, 737
HpyCH4III ACNGT 3 cut(s) 272, 295, 560
HpyCH4IV ACGT 2 cut(s) 547, 662
HpyCH4V TGCA 5 cut(s) 61, 66, 505, 791, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 511
HpyF3I CTNAG 2 cut(s) 347, 681
HpySE526I ACGT 2 cut(s) 547, 662
KflI GGGWCCC 1 cut(s) 584
Kzo9I GATC 4 cut(s) 196, 438, 595, 619
LmnI GCTCC 2 cut(s) 205, 886
LweI GCATC 1 cut(s) 833
MaeI CTAG 1 cut(s) 720
MaeII ACGT 2 cut(s) 547, 662
MaeIII GTNAC 5 cut(s) 160, 295, 775, 854, 904
MalI GATC 4 cut(s) 198, 440, 597, 621
MbiI CCGCTC 1 cut(s) 469
MboI GATC 4 cut(s) 196, 438, 595, 619
MboII GAAGA 3 cut(s) 140, 448, 924
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 4 cut(s) 99, 233, 727, 793
MlsI TGGCCA 1 cut(s) 669
MluCI AATT 3 cut(s) 188, 550, 796
MluNI TGGCCA 1 cut(s) 669
Mox20I TGGCCA 1 cut(s) 669
MscI TGGCCA 1 cut(s) 669
MseI TTAA 3 cut(s) 122, 690, 707
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 733
MspR9I CCNGG 1 cut(s) 671
MvaI CCWGG 1 cut(s) 671
MwoI GCNNNNNNNGC 2 cut(s) 67, 511
NcoI CCATGG 1 cut(s) 25
NdeII GATC 4 cut(s) 196, 438, 595, 619
NlaIV GGNNCC 4 cut(s) 12, 573, 585, 586
NmuCI GTSAC 3 cut(s) 775, 854, 904
NspV TTCGAA 1 cut(s) 17
PagI TCATGA 2 cut(s) 577, 760
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 3 cut(s) 5, 31, 485
PflMI CCANNNNNTGG 2 cut(s) 199, 432
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 584
PshBI ATTAAT 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 669
PspGI CCWGG 1 cut(s) 669
PspN4I GGNNCC 4 cut(s) 12, 573, 585, 586
PspPI GGNCC 3 cut(s) 10, 268, 584
PspPPI RGGWCCY 1 cut(s) 584
PsrI GAACNNNNNNTAC 2 cut(s) 555, 587
PsuI RGATCY 1 cut(s) 438
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
SaqAI TTAA 3 cut(s) 122, 690, 707
Sau3AI GATC 4 cut(s) 196, 438, 595, 619
Sau96I GGNCC 3 cut(s) 10, 268, 584
ScrFI CCNGG 1 cut(s) 671
SduI GDGCHC 4 cut(s) 99, 233, 727, 793
SfaNI GCATC 1 cut(s) 833
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 3 cut(s) 10, 268, 584
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 3 cut(s) 188, 550, 796
SsiI CCGC 3 cut(s) 203, 214, 467
SspI AATATT 2 cut(s) 120, 495
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 1 cut(s) 669
StyI CCWWGG 2 cut(s) 25, 111
TaaI ACNGT 3 cut(s) 272, 295, 560
TaiI ACGT 2 cut(s) 550, 665
TaqI TCGA 3 cut(s) 17, 537, 840
TasI AATT 3 cut(s) 188, 550, 796
TatI WGTACW 1 cut(s) 562
TfiI GAWTC 3 cut(s) 5, 31, 485
Tru1I TTAA 3 cut(s) 122, 690, 707
Tru9I TTAA 3 cut(s) 122, 690, 707
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 3 cut(s) 775, 854, 904
Tsp45I GTSAC 3 cut(s) 775, 854, 904
TspDTI ATGAA 4 cut(s) 170, 375, 777, 853
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 1 cut(s) 68
Van91I CCANNNNNTGG 2 cut(s) 199, 432
VneI GTGCAC 1 cut(s) 789
VpaK11BI GGWCC 3 cut(s) 10, 268, 584
VspI ATTAAT 1 cut(s) 122
XspI CTAG 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.