Rh3AG220100

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002422
Physical Location & Seq
Reverse (-)
22469989 .. 22477004
7016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG220100.1

Sequence Viewer

Length: 684 bp
ATGGGAGATTATGTGGACCGTGGCTATTATTCGGTTGAAACTGTAACGCTTCTAGTGGCCCTGAAAGTGCGTTATCATCAGCGCATCACTATTCTTAGAGGAAACCATGAAAGTCGTCAGATTACTCAGGTTTATGGGTTTTATGATGAATGTCTTCGCAAGTATGGGAACGCCAGTGTATGGAAGACCTTCACCGACCTTTTTGACTACTTTCCACTGACAGCATTGGTTGAGTCAGAGATATTTTGTCTGCATGGTGGATTATCACCTTCAATCGAAAACCTTGATAACATAAGGAATTTTGATCGTGTTCAAGAGGTTCCGCATGAAGGGCCCATGTGTGATCTGTTATGGTCTGACCCAGATGACCGATGTGGTTGGGGTATTTCACCTCGCGGTGCAGGATATACCTTTGGCCAGGATATATCTGAACAATTCAATCATACAAATAGTTTAAAGTTGATTGCAAGAGCTCATCAATTGGTTATGGATGGATTCAACTGGGCCCATGAACAAAAGGTGGTTACCATATTCAGTGCACCTAATTATTGCTATCGCTGTGGGAACATGGCGTCTGTTTTAGAAGTTGATGACTGCAGGGGCCATACTTTCATCCAGTTTGAGCCTGCTCCTAGGAGAGGGGAACCGGATGTCACTCGTAGAACACCTGATTACTTTTTATGA

Protein Analysis

227

Amino Acids

26.18

Weight (kDa)

5.45

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 2 - 162 2.5e-35 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AccII CGCG 1 cut(s) 396
AciI CCGC 2 cut(s) 323, 396
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 572
AfiI CCNNNNNNNGG 3 cut(s) 180, 329, 638
AgsI TTSAA 5 cut(s) 38, 273, 314, 439, 499
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 1 cut(s) 473
AluI AGCT 1 cut(s) 473
Alw21I GWGCWC 2 cut(s) 475, 541
Alw44I GTGCAC 1 cut(s) 537
AoxI GGCC 5 cut(s) 57, 332, 415, 504, 601
ApaI GGGCCC 2 cut(s) 336, 508
ApaLI GTGCAC 1 cut(s) 537
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 2 cut(s) 153, 188
AspA2I CCTAGG 1 cut(s) 632
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 7 cut(s) 16, 58, 332, 333, 504, 505, 601
AsuHPI GGTGA 3 cut(s) 184, 258, 381
AvaII GGWCC 1 cut(s) 16
AvrII CCTAGG 1 cut(s) 632
BaeGI GKGCMC 3 cut(s) 336, 508, 541
BalI TGGCCA 1 cut(s) 417
BanII GRGCYC 3 cut(s) 336, 475, 508
BbsI GAAGAC 2 cut(s) 146, 191
Bbv12I GWGCWC 2 cut(s) 475, 541
BccI CCATC 1 cut(s) 485
BciT130I CCWGG 1 cut(s) 419
BfaI CTAG 2 cut(s) 53, 633
BfmI CTRYAG 1 cut(s) 595
BlnI CCTAGG 1 cut(s) 632
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 7 cut(s) 16, 58, 332, 333, 504, 505, 601
BmiI GGNNCC 5 cut(s) 321, 334, 506, 602, 645
BmrFI CCNGG 1 cut(s) 419
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 511
BpiI GAAGAC 2 cut(s) 146, 191
BsaHI GRCGYC 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 19, 632
BsaWI WCCGGW 1 cut(s) 646
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 329, 638
Bse1I ACTGG 3 cut(s) 174, 506, 616
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 19, 632
BseGI GGATG 3 cut(s) 496, 612, 655
BseLI CCNNNNNNNGG 3 cut(s) 180, 329, 638
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 3 cut(s) 174, 506, 616
BseSI GKGCMC 3 cut(s) 336, 508, 541
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 1 cut(s) 396
BshFI GGCC 5 cut(s) 59, 334, 417, 506, 603
BsiHKAI GWGCWC 2 cut(s) 475, 541
BsiSI CCGG 1 cut(s) 647
BslI CCNNNNNNNGG 3 cut(s) 180, 329, 638
BsnI GGCC 5 cut(s) 59, 334, 417, 506, 603
Bsp120I GGGCCC 2 cut(s) 332, 504
Bsp1286I GDGCHC 4 cut(s) 336, 475, 508, 541
Bsp143I GATC 2 cut(s) 304, 343
BspACI CCGC 2 cut(s) 323, 396
BspANI GGCC 5 cut(s) 59, 334, 417, 506, 603
BspCNI CTCAG 1 cut(s) 139
BspFNI CGCG 1 cut(s) 396
BspLI GGNNCC 5 cut(s) 321, 334, 506, 602, 645
BspMAI CTGCAG 1 cut(s) 599
BsrI ACTGG 3 cut(s) 174, 506, 616
BssECI CCNNGG 2 cut(s) 19, 632
BssMI GATC 2 cut(s) 304, 343
BssNI GRCGYC 1 cut(s) 572
BssT1I CCWWGG 1 cut(s) 632
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 2 cut(s) 20, 43
BstACI GRCGYC 1 cut(s) 572
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 2 cut(s) 95, 126
BstDSI CCRYGG 1 cut(s) 19
BstEII GGTNACC 1 cut(s) 523
BstENI CCTNNNNNAGG 1 cut(s) 636
BstF5I GGATG 3 cut(s) 496, 612, 655
BstFNI CGCG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 2 cut(s) 307, 346
BstMBI GATC 2 cut(s) 304, 343
BstMWI GCNNNNNNNGC 1 cut(s) 331
BstNI CCWGG 1 cut(s) 419
BstPI GGTNACC 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 417
BstSFI CTRYAG 1 cut(s) 595
BstSLI GKGCMC 3 cut(s) 336, 508, 541
BstUI CGCG 1 cut(s) 396
BstV2I GAAGAC 2 cut(s) 146, 191
BsuRI GGCC 5 cut(s) 59, 334, 417, 506, 603
BtgI CCRYGG 1 cut(s) 19
BtsCI GGATG 3 cut(s) 496, 612, 655
BtsIMutI CAGTG 3 cut(s) 181, 215, 541
Cac8I GCNNGC 1 cut(s) 627
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 7 cut(s) 16, 58, 332, 333, 504, 505, 601
CseI GACGC 1 cut(s) 561
CviAII CATG 6 cut(s) 107, 254, 326, 337, 509, 568
CviJI RGCY 8 cut(s) 24, 59, 334, 417, 473, 506, 603, 625
CviKI_1 RGCY 8 cut(s) 24, 59, 334, 417, 473, 506, 603, 625
DdeI CTNAG 2 cut(s) 95, 126
DpnI GATC 2 cut(s) 306, 345
DpnII GATC 2 cut(s) 304, 343
DraI TTTAAA 1 cut(s) 456
EaeI YGGCCR 1 cut(s) 415
Ecl136II GAGCTC 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 632
Eco24I GRGCYC 3 cut(s) 336, 475, 508
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 473
Eco91I GGTNACC 1 cut(s) 523
EcoICRI GAGCTC 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 332
EcoO65I GGTNACC 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 632
EcoT38I GRGCYC 3 cut(s) 336, 475, 508
ErhI CCWWGG 1 cut(s) 632
FaeI CATG 6 cut(s) 110, 257, 329, 340, 512, 571
FatI CATG 6 cut(s) 106, 253, 325, 336, 508, 567
FokI GGATG 3 cut(s) 503, 599, 662
FriOI GRGCYC 3 cut(s) 336, 475, 508
FspBI CTAG 2 cut(s) 53, 633
GlaI GCGC 1 cut(s) 83
HaeIII GGCC 5 cut(s) 59, 334, 417, 506, 603
HapII CCGG 1 cut(s) 647
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 84
Hin1I GRCGYC 1 cut(s) 572
Hin1II CATG 6 cut(s) 110, 257, 329, 340, 512, 571
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HinfI GANTC 2 cut(s) 233, 495
HpaII CCGG 1 cut(s) 647
HphI GGTGA 3 cut(s) 184, 258, 381
Hpy166II GTNNAC 2 cut(s) 16, 539
Hpy188I TCNGA 4 cut(s) 120, 238, 358, 430
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 2 cut(s) 16, 539
HpyAV CCTTC 3 cut(s) 199, 279, 323
HpyCH4III ACNGT 2 cut(s) 20, 43
HpyCH4V TGCA 5 cut(s) 253, 401, 467, 539, 597
HpyF10VI GCNNNNNNNGC 1 cut(s) 331
HpyF3I CTNAG 2 cut(s) 95, 126
Hsp92I GRCGYC 1 cut(s) 572
Hsp92II CATG 6 cut(s) 110, 257, 329, 340, 512, 571
HspAI GCGC 1 cut(s) 82
Kzo9I GATC 2 cut(s) 304, 343
LmnI GCTCC 1 cut(s) 634
LweI GCATC 1 cut(s) 93
MaeI CTAG 2 cut(s) 53, 633
MaeIII GTNAC 3 cut(s) 43, 523, 652
MalI GATC 2 cut(s) 306, 345
MboI GATC 2 cut(s) 304, 343
MboII GAAGA 2 cut(s) 146, 196
MfeI CAATTG 1 cut(s) 479
MhlI GDGCHC 4 cut(s) 336, 475, 508, 541
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 4 cut(s) 298, 434, 479, 544
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 4 cut(s) 92, 310, 402, 632
Mox20I TGGCCA 1 cut(s) 417
MroXI GAANNNNTTC 2 cut(s) 153, 188
MscI TGGCCA 1 cut(s) 417
MseI TTAA 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 417
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 1 cut(s) 419
MunI CAATTG 1 cut(s) 479
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 1 cut(s) 396
MwoI GCNNNNNNNGC 1 cut(s) 331
NdeII GATC 2 cut(s) 304, 343
NlaIII CATG 6 cut(s) 110, 257, 329, 340, 512, 571
NlaIV GGNNCC 5 cut(s) 321, 334, 506, 602, 645
NmuCI GTSAC 1 cut(s) 652
PdmI GAANNNNTTC 2 cut(s) 153, 188
PfeI GAWTC 1 cut(s) 495
PflMI CCANNNNNTGG 1 cut(s) 180
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 417
PspEI GGTNACC 1 cut(s) 523
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 5 cut(s) 321, 334, 506, 602, 645
PspOMI GGGCCC 2 cut(s) 332, 504
PspPI GGNCC 7 cut(s) 16, 58, 332, 333, 504, 505, 601
PstI CTGCAG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 475
SaqAI TTAA 1 cut(s) 455
Sau3AI GATC 2 cut(s) 304, 343
Sau96I GGNCC 7 cut(s) 16, 58, 332, 333, 504, 505, 601
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 4 cut(s) 336, 475, 508, 541
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 1 cut(s) 595
SinI GGWCC 1 cut(s) 16
Sse9I AATT 4 cut(s) 298, 434, 479, 544
SsiI CCGC 2 cut(s) 323, 396
SspMI CTAG 2 cut(s) 53, 633
SstI GAGCTC 1 cut(s) 475
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 2 cut(s) 20, 43
TaqI TCGA 1 cut(s) 276
TaqII GACCGA 1 cut(s) 384
TasI AATT 4 cut(s) 298, 434, 479, 544
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 1 cut(s) 455
Tru9I TTAA 1 cut(s) 455
TscAI CASTG 3 cut(s) 181, 222, 541
TseFI GTSAC 1 cut(s) 652
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 5 cut(s) 123, 162, 342, 525, 601
TspRI CASTG 3 cut(s) 181, 222, 541
Van91I CCANNNNNTGG 1 cut(s) 180
VneI GTGCAC 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 16
XagI CCTNNNNNAGG 1 cut(s) 636
XapI RAATTY 1 cut(s) 298
XmaJI CCTAGG 1 cut(s) 632
XmnI GAANNNNTTC 2 cut(s) 153, 188
XspI CTAG 2 cut(s) 53, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.