RLG00000036992

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002422
Physical Location & Seq
Forward (+)
87375365 .. 87379745
4381 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036992

Sequence Viewer

Length: 936 bp
ATGGATTCGGTCCCTTCGAACTCGCTTGGTAACCTCGACGAGCAGATTGCTCAGCTCATGCAGTGCAAGCCTTTGTCCGAACAAGAGGTAAGGGGTCTCTGTGAAAAGGCTAAGGAGATATTGATGGAAGAAAGCAATGTTCAGCCTGTGAAAAGCCCAGTAACTATATGCGGTGATATTCATGGACAATTTCATGATCTTGCCGAGCTTTTCCGTATTGGAGGAAAGTGTCCAGATACCAATTATTTGTTTATGGGAGATTATGTGGATCGTGGCTATTATTCCGTTGAAACCGTAACTCTTTTGGTGGCGTTGAAGGTTCGTTACTCTCAACGTATAACCATCCTAAGAGGGAATCATGAAAGTCGTCAGATTACTCAAGTTTATGGTTTTTATGACGAGTGCTTACGGAAGTATGGTAATGCCAATGTTTGGAAGACCTTTACTGATTTATTTGACTATTTTCCGCTGACAGCTTTGGTTGAATCTGAAATCTTCTGCTTGCACGGCGGACTTTCTCCCTCAATTGAAACCCTTGATAACATACGTAATTTTGACCGTGTTCAAGAGGTTCCTCATGAAGGTCCCATGTGTGATCTTCTTTGGTCTGACCCAGATGATCGCTGTGGCTGGGGTATCTCTCCCAGAGGTGCTGGATATACTTTTGGGCAGGACATATCCGAGCAGTTCAATCATACCAATAACTTGAAGTTGATTGCTAGAGCACACCAGCTGGTCATGGAAGGTTATAACTGGGGTCATGAACAAAAAGTTGTCACCATATTCAGTGCACCCAACTACTGTTACCGCTGTGGAAATATGGCATCAATCCTAGAAGTCGATGACTGCAAAGGTCACACATTCATTCAGTTTGAGCCAGCTCCAAGGAGGGGTGAGCCAGATGTTACCCGAAGAACACCTGATTATTTCCTATAA

Protein Analysis

312

Amino Acids

35.49

Weight (kDa)

5.08

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 53 - 246 2.3e-47 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AccB7I CCANNNNNTGG 1 cut(s) 432
AciI CCGC 4 cut(s) 171, 467, 510, 808
AclWI GGATC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 432, 581, 890
AgsI TTSAA 7 cut(s) 290, 316, 485, 530, 566, 691, 709
AluBI AGCT 5 cut(s) 55, 208, 476, 733, 881
AluI AGCT 5 cut(s) 55, 208, 476, 733, 881
Alw21I GWGCWC 2 cut(s) 727, 793
Alw26I GTCTC 1 cut(s) 101
Alw44I GTGCAC 1 cut(s) 789
AlwI GGATC 1 cut(s) 276
ApaLI GTGCAC 1 cut(s) 789
AspS9I GGNCC 2 cut(s) 10, 584
AsuHPI GGTGA 3 cut(s) 185, 769, 905
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 2 cut(s) 10, 584
BaeGI GKGCMC 1 cut(s) 793
BarI GAAGNNNNNNTAC 2 cut(s) 308, 340
BbsI GAAGAC 1 cut(s) 443
Bbv12I GWGCWC 2 cut(s) 727, 793
BccI CCATC 2 cut(s) 118, 350
BceAI ACGGC 1 cut(s) 523
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 720, 833
BlpI GCTNAGC 1 cut(s) 51
Bme18I GGWCC 2 cut(s) 10, 584
BmgT120I GGNCC 2 cut(s) 10, 584
BmiI GGNNCC 3 cut(s) 12, 573, 586
BmrI ACTGGG 2 cut(s) 152, 763
BmsI GCATC 1 cut(s) 833
BmuI ACTGGG 2 cut(s) 152, 763
BpiI GAAGAC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 548
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 884
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 3 cut(s) 432, 581, 890
Bse1I ACTGG 2 cut(s) 158, 758
Bse3DI GCAATG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 884
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 3 cut(s) 432, 581, 890
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 2 cut(s) 158, 758
BseSI GKGCMC 1 cut(s) 793
BseYI CCCAGC 1 cut(s) 630
BsiHKAI GWGCWC 2 cut(s) 727, 793
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 3 cut(s) 432, 581, 890
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 1 cut(s) 570
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 727, 793
Bsp143I GATC 4 cut(s) 196, 268, 595, 619
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 4 cut(s) 171, 467, 510, 808
BspCNI CTCAG 1 cut(s) 64
BspHI TCATGA 4 cut(s) 193, 358, 577, 760
BspLI GGNNCC 3 cut(s) 12, 573, 586
BspPI GGATC 1 cut(s) 276
BspT104I TTCGAA 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 142
BsrI ACTGG 2 cut(s) 158, 758
BssECI CCNNGG 1 cut(s) 884
BssMI GATC 4 cut(s) 196, 268, 595, 619
BssT1I CCWWGG 1 cut(s) 884
Bst4CI ACNGT 3 cut(s) 295, 560, 803
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 3 cut(s) 68, 503, 879
BstDEI CTNAG 3 cut(s) 51, 111, 347
BstEII GGTNACC 1 cut(s) 29
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 1 cut(s) 342
BstKTI GATC 4 cut(s) 199, 271, 598, 622
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 4 cut(s) 196, 268, 595, 619
BstMWI GCNNNNNNNGC 2 cut(s) 67, 507
BstPI GGTNACC 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 793
BstSNI TACGTA 1 cut(s) 548
BstV2I GAAGAC 1 cut(s) 443
BtsCI GGATG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 68, 793
Cac8I GCNNGC 3 cut(s) 68, 503, 879
CciI TCATGA 4 cut(s) 193, 358, 577, 760
Cfr13I GGNCC 2 cut(s) 10, 584
CviAII CATG 8 cut(s) 58, 182, 194, 359, 578, 589, 739, 761
DdeI CTNAG 3 cut(s) 51, 111, 347
DpnI GATC 4 cut(s) 198, 270, 597, 621
DpnII GATC 4 cut(s) 196, 268, 595, 619
EciI GGCGGA 1 cut(s) 525
Eco105I TACGTA 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 884
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 2 cut(s) 10, 584
Eco91I GGTNACC 1 cut(s) 29
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 584
EcoO65I GGTNACC 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 884
ErhI CCWWGG 1 cut(s) 884
FaeI CATG 8 cut(s) 61, 185, 197, 362, 581, 592, 742, 764
FaqI GGGAC 1 cut(s) 570
FatI CATG 8 cut(s) 57, 181, 193, 358, 577, 588, 738, 760
FokI GGATG 1 cut(s) 329
FspBI CTAG 2 cut(s) 720, 833
GsaI CCCAGC 1 cut(s) 634
Hin1II CATG 8 cut(s) 61, 185, 197, 362, 581, 592, 742, 764
HinfI GANTC 3 cut(s) 5, 355, 485
HphI GGTGA 3 cut(s) 185, 769, 905
Hpy166II GTNNAC 1 cut(s) 791
Hpy188I TCNGA 5 cut(s) 79, 372, 490, 610, 682
Hpy188III TCNNGA 6 cut(s) 194, 233, 359, 566, 578, 761
Hpy8I GTNNAC 1 cut(s) 791
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 24, 310, 575, 737
HpyCH4III ACNGT 3 cut(s) 295, 560, 803
HpyCH4IV ACGT 2 cut(s) 334, 547
HpyCH4V TGCA 5 cut(s) 61, 66, 505, 791, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 507
HpyF3I CTNAG 3 cut(s) 51, 111, 347
HpySE526I ACGT 2 cut(s) 334, 547
Hsp92II CATG 8 cut(s) 61, 185, 197, 362, 581, 592, 742, 764
Kzo9I GATC 4 cut(s) 196, 268, 595, 619
LmnI GCTCC 1 cut(s) 886
LweI GCATC 1 cut(s) 833
MaeI CTAG 2 cut(s) 720, 833
MaeII ACGT 2 cut(s) 334, 547
MaeIII GTNAC 8 cut(s) 29, 160, 295, 323, 775, 803, 854, 904
MalI GATC 4 cut(s) 198, 270, 597, 621
MboI GATC 4 cut(s) 196, 268, 595, 619
MboII GAAGA 5 cut(s) 140, 448, 487, 590, 924
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 2 cut(s) 727, 793
MluCI AATT 4 cut(s) 188, 241, 525, 550
MnlI CCTC 9 cut(s) 44, 79, 215, 344, 532, 562, 585, 641, 882
MspA1I CMGCKG 3 cut(s) 469, 733, 810
MunI CAATTG 1 cut(s) 525
MwoI GCNNNNNNNGC 2 cut(s) 67, 507
NdeII GATC 4 cut(s) 196, 268, 595, 619
NlaIII CATG 8 cut(s) 61, 185, 197, 362, 581, 592, 742, 764
NlaIV GGNNCC 3 cut(s) 12, 573, 586
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 2 cut(s) 775, 854
NspV TTCGAA 1 cut(s) 17
PagI TCATGA 4 cut(s) 193, 358, 577, 760
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 3 cut(s) 5, 355, 485
PflMI CCANNNNNTGG 1 cut(s) 432
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 584
PsiI TTATAA 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 584
PspEI GGTNACC 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 630
PspN4I GGNNCC 3 cut(s) 12, 573, 586
PspPI GGNCC 2 cut(s) 10, 584
PspPPI RGGWCCY 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 733
Sau3AI GATC 4 cut(s) 196, 268, 595, 619
Sau96I GGNCC 2 cut(s) 10, 584
SduI GDGCHC 2 cut(s) 727, 793
SfaNI GCATC 1 cut(s) 833
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 2 cut(s) 10, 584
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 4 cut(s) 188, 241, 525, 550
SsiI CCGC 4 cut(s) 171, 467, 510, 808
SspMI CTAG 2 cut(s) 720, 833
StyI CCWWGG 1 cut(s) 884
TaaI ACNGT 3 cut(s) 295, 560, 803
TaiI ACGT 2 cut(s) 337, 550
TaqI TCGA 3 cut(s) 17, 36, 840
TasI AATT 4 cut(s) 188, 241, 525, 550
TfiI GAWTC 3 cut(s) 5, 355, 485
TscAI CASTG 2 cut(s) 68, 793
TseFI GTSAC 2 cut(s) 775, 854
Tsp45I GTSAC 2 cut(s) 775, 854
TspDTI ATGAA 6 cut(s) 170, 182, 375, 594, 777, 853
TspGWI ACGGA 3 cut(s) 203, 274, 424
TspRI CASTG 2 cut(s) 68, 793
Van91I CCANNNNNTGG 1 cut(s) 432
VneI GTGCAC 1 cut(s) 789
VpaK11BI GGWCC 2 cut(s) 10, 584
XagI CCTNNNNNAGG 1 cut(s) 579
XspI CTAG 2 cut(s) 720, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.