Rorug05G0480100

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002422
Physical Location & Seq
Forward (+)
66139584 .. 66143088
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0480100.1

Sequence Viewer

Length: 765 bp
ATGGATTGTACTCACCATTGTCTCACCACCATAGTATTCTACAGAGTTGTCCAGAGCAGTTCTGAAAATGTTTTGTTAAGAAGTTGCAAAAGGAGTTTCAATGGATTTGCTGCCAAGCTCTCGGACCAGGAACGAGAAAAACTTTCTAACATGAAGGAAGTAGTCTCTGTCTTTCCGAGCAGAATATTGCAACTTCAGACAACAAGATCTTGGGATTTTATGGGTTTGAATGAGAAAGTTAGTCGAAATGCCACTGTTGAGAGTGATATCATTGTTGGTGTGATTGACACTGGAATTTGGCCCGAGTCTGAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCTAAGAAGTGGAAAGGTGCTTGTGAAGGGGGAAAAAAATTTACTTGCAACAAGTATGTTCCCTCACTATTCATTAATTCATCTCTTGGCCCCTCCATAACCACACTATATGATCCTGCACTATGGATACTGACAGTTGCAGCAAGTAGCACAGATCGGCGGATCATTGACAAGGTTGTTCTTGGAAATGGTAGTATACTAGTGGGAAATTATGTTAACACTTTCACATTAAATGGAACTAGTCTTCCTCTGATACATGGAAAAGATGTTTCACGTAATTGCACTGAACGAGACGCTAGTGATTGTGTTGAAGGCTGCCTGGACAGTGATCTAGTAAAGGTCAAGATTGTGTTATGTGATGACTACATTAATGAAAATCTTGAGGCATATAAAGCTGGACGCACTGGGTTCAGTTTTATATAA

Protein Analysis

254

Amino Acids

28.11

Weight (kDa)

6.32

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 19 - 66 5.7e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 538
AciI CCGC 1 cut(s) 502
AclWI GGATC 2 cut(s) 449, 512
AcsI RAATTY 2 cut(s) 294, 380
AcuI CTGAAG 1 cut(s) 179
AfaI GTAC 1 cut(s) 10
AgsI TTSAA 3 cut(s) 100, 229, 653
AhlI ACTAGT 2 cut(s) 541, 581
AjnI CCWGG 2 cut(s) 126, 660
AloI GAACNNNNNNTCC 2 cut(s) 571, 603
AluBI AGCT 3 cut(s) 118, 315, 737
AluI AGCT 3 cut(s) 118, 315, 737
Alw26I GTCTC 3 cut(s) 26, 169, 627
AlwI GGATC 2 cut(s) 449, 512
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 2 cut(s) 299, 430
ApeKI GCWGC 3 cut(s) 110, 482, 657
ApoI RAATTY 2 cut(s) 294, 380
AseI ATTAAT 2 cut(s) 417, 711
AspS9I GGNCC 4 cut(s) 124, 300, 335, 431
AsuHPI GGTGA 2 cut(s) 5, 16
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 2 cut(s) 124, 335
BbsI GAAGAC 1 cut(s) 578
BbvI GCAGC 3 cut(s) 97, 494, 644
BccI CCATC 1 cut(s) 320
BciT130I CCWGG 2 cut(s) 128, 662
BciVI GTATCC 1 cut(s) 462
BcoDI GTCTC 3 cut(s) 26, 169, 627
BcuI ACTAGT 2 cut(s) 541, 581
BfaI CTAG 4 cut(s) 542, 582, 639, 674
BfmI CTRYAG 1 cut(s) 40
BfuI GTATCC 1 cut(s) 462
BglII AGATCT 1 cut(s) 206
BisI GCNGC 3 cut(s) 111, 483, 658
BlsI GCNGC 3 cut(s) 112, 484, 659
Bme1390I CCNGG 2 cut(s) 128, 662
Bme18I GGWCC 2 cut(s) 124, 335
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 4 cut(s) 124, 300, 335, 431
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 2 cut(s) 128, 662
BmrI ACTGGG 1 cut(s) 756
BmuI ACTGGG 1 cut(s) 756
BpiI GAAGAC 1 cut(s) 578
BpuEI CTTGAG 1 cut(s) 743
BsaAI YACGTR 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 295, 751
BseBI CCWGG 2 cut(s) 128, 662
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 295, 751
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 3 cut(s) 97, 494, 644
BsgI GTGCAG 1 cut(s) 444
BshFI GGCC 2 cut(s) 301, 432
BsiHKCI CYCGRG 1 cut(s) 302
BsmAI GTCTC 3 cut(s) 26, 169, 627
BsmBI CGTCTC 1 cut(s) 627
BsnI GGCC 2 cut(s) 301, 432
BsoBI CYCGRG 1 cut(s) 302
Bsp143I GATC 5 cut(s) 206, 454, 496, 504, 670
BspACI CCGC 1 cut(s) 502
BspANI GGCC 2 cut(s) 301, 432
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 433
BspPI GGATC 2 cut(s) 449, 512
BsrI ACTGG 2 cut(s) 295, 751
BssMI GATC 5 cut(s) 206, 454, 496, 504, 670
BssNAI GTATAC 1 cut(s) 539
Bst1107I GTATAC 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 128, 662
Bst4CI ACNGT 3 cut(s) 256, 478, 668
BstBAI YACGTR 1 cut(s) 617
BstDEI CTNAG 2 cut(s) 309, 345
BstKTI GATC 5 cut(s) 209, 457, 499, 507, 673
BstMAI GTCTC 3 cut(s) 26, 169, 627
BstMBI GATC 5 cut(s) 206, 454, 496, 504, 670
BstMWI GCNNNNNNNGC 1 cut(s) 734
BstNI CCWGG 2 cut(s) 128, 662
BstSCI CCNGG 2 cut(s) 126, 660
BstSFI CTRYAG 1 cut(s) 40
BstV1I GCAGC 3 cut(s) 97, 494, 644
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BstZ17I GTATAC 1 cut(s) 539
BsuI GTATCC 1 cut(s) 462
BsuRI GGCC 2 cut(s) 301, 432
BtsIMutI CAGTG 5 cut(s) 252, 288, 624, 673, 744
Cfr13I GGNCC 4 cut(s) 124, 300, 335, 431
CseI GACGC 2 cut(s) 644, 750
Csp6I GTAC 1 cut(s) 9
CviAII CATG 2 cut(s) 151, 599
CviJI RGCY 6 cut(s) 118, 301, 315, 432, 657, 737
CviKI_1 RGCY 6 cut(s) 118, 301, 315, 432, 657, 737
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 2 cut(s) 309, 345
DpnI GATC 5 cut(s) 208, 456, 498, 506, 672
DpnII GATC 5 cut(s) 206, 454, 496, 504, 670
DraI TTTAAA 1 cut(s) 319
EciI GGCGGA 1 cut(s) 517
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 2 cut(s) 124, 335
Eco57I CTGAAG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 302
EcoRII CCWGG 2 cut(s) 126, 660
EcoRV GATATC 1 cut(s) 268
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 2 cut(s) 154, 602
FatI CATG 2 cut(s) 150, 598
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 3 cut(s) 111, 483, 658
Fsp4HI GCNGC 3 cut(s) 111, 483, 658
FspBI CTAG 4 cut(s) 542, 582, 639, 674
GluI GCNGC 3 cut(s) 111, 483, 658
HaeIII GGCC 2 cut(s) 301, 432
HgaI GACGC 2 cut(s) 644, 750
Hin1II CATG 2 cut(s) 154, 602
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 1 cut(s) 305
HpaI GTTAAC 1 cut(s) 559
HphI GGTGA 2 cut(s) 5, 16
Hpy166II GTNNAC 2 cut(s) 539, 559
Hpy188I TCNGA 6 cut(s) 64, 124, 177, 198, 310, 594
Hpy188III TCNNGA 3 cut(s) 52, 685, 722
Hpy8I GTNNAC 2 cut(s) 539, 559
HpyAV CCTTC 3 cut(s) 148, 362, 647
HpyCH4III ACNGT 3 cut(s) 256, 478, 668
HpyCH4IV ACGT 1 cut(s) 616
HpyCH4V TGCA 6 cut(s) 87, 190, 390, 461, 482, 624
HpyF10VI GCNNNNNNNGC 1 cut(s) 734
HpyF3I CTNAG 2 cut(s) 309, 345
HpySE526I ACGT 1 cut(s) 616
Hsp92II CATG 2 cut(s) 154, 602
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 5 cut(s) 206, 454, 496, 504, 670
LpnPI CCDG 9 cut(s) 65, 113, 140, 276, 471, 647, 674, 723, 732
Lsp1109I GCAGC 3 cut(s) 97, 494, 644
MaeI CTAG 4 cut(s) 542, 582, 639, 674
MaeII ACGT 1 cut(s) 616
MalI GATC 5 cut(s) 208, 456, 498, 506, 672
MboI GATC 5 cut(s) 206, 454, 496, 504, 670
MboII GAAGA 1 cut(s) 578
MflI RGATCY 1 cut(s) 206
MluCI AATT 5 cut(s) 294, 380, 418, 550, 619
MlyI GAGTC 1 cut(s) 314
MnlI CCTC 6 cut(s) 348, 351, 415, 445, 600, 718
MseI TTAA 6 cut(s) 77, 318, 417, 558, 572, 711
MspR9I CCNGG 2 cut(s) 128, 662
MvaI CCWGG 2 cut(s) 128, 662
MwoI GCNNNNNNNGC 1 cut(s) 734
NdeII GATC 5 cut(s) 206, 454, 496, 504, 670
NlaIII CATG 2 cut(s) 154, 602
NlaIV GGNNCC 1 cut(s) 433
PkrI GCNGC 3 cut(s) 112, 484, 659
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Ppu21I YACGTR 1 cut(s) 617
PshBI ATTAAT 2 cut(s) 417, 711
Psp6I CCWGG 2 cut(s) 126, 660
PspGI CCWGG 2 cut(s) 126, 660
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 4 cut(s) 124, 300, 335, 431
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
SaqAI TTAA 6 cut(s) 77, 318, 417, 558, 572, 711
SatI GCNGC 3 cut(s) 111, 483, 658
Sau3AI GATC 5 cut(s) 206, 454, 496, 504, 670
Sau96I GGNCC 4 cut(s) 124, 300, 335, 431
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 128, 662
SetI ASST 7 cut(s) 120, 317, 361, 519, 619, 684, 739
SfcI CTRYAG 1 cut(s) 40
SinI GGWCC 2 cut(s) 124, 335
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
SpeI ACTAGT 2 cut(s) 541, 581
Sse9I AATT 5 cut(s) 294, 380, 418, 550, 619
SsiI CCGC 1 cut(s) 502
SspI AATATT 1 cut(s) 186
SspMI CTAG 4 cut(s) 542, 582, 639, 674
StyD4I CCNGG 2 cut(s) 126, 660
TaaI ACNGT 3 cut(s) 256, 478, 668
TaiI ACGT 1 cut(s) 619
TaqI TCGA 1 cut(s) 244
TasI AATT 5 cut(s) 294, 380, 418, 550, 619
TatI WGTACW 1 cut(s) 8
Tru1I TTAA 6 cut(s) 77, 318, 417, 558, 572, 711
Tru9I TTAA 6 cut(s) 77, 318, 417, 558, 572, 711
TscAI CASTG 5 cut(s) 259, 295, 631, 673, 751
TseI GCWGC 3 cut(s) 110, 482, 657
TspDTI ATGAA 4 cut(s) 167, 403, 411, 729
TspRI CASTG 5 cut(s) 259, 295, 631, 673, 751
VpaK11BI GGWCC 2 cut(s) 124, 335
VspI ATTAAT 2 cut(s) 417, 711
XapI RAATTY 2 cut(s) 294, 380
XmiI GTMKAC 1 cut(s) 538
XspI CTAG 4 cut(s) 542, 582, 639, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.