Rh3DG245000

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002422
Physical Location & Seq
Reverse (-)
23902984 .. 23909703
6720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG245000.1

Sequence Viewer

Length: 813 bp
ATGGAAGAAAGCAATGTCCAGCCTGTGAAAAGCCCAGTGACAATTTGTGGCGATATTCATGGGCAGTTTCACGACCTTGCGGAGCTCTTTCGAATTGGAGGGAAGTGTCCCGATACCAATTACTTATTTATGGGAGATTATGTGGACCGTGGCTATTATTCGGTTGAAACTGTAACGCTTCTAGTGGCCCTGAAAGTGCGTTATCATCAGCGCATCACTATTCTTAGAGGAAACCATGAAAGTCGTCAGATTACTCAGGTTTATGGGTTTTATGATGAATGTCTTCGCAAGTATGGGAACGCCAGTGTATGGAAGACCTTCACCGACCTTTTTGACTACTTTCCACTGACAGCATTGGTTGAGTCAGAGATATTTTGTCTGCATGGTGGATTATCACCTTCAATCGAAAACCTTGATAACATAAGGAATTTTGATCGTGTTCAAGAGGTTCCGCATGAAGGCCCCATGTGTGATCTGTTATGGTCTGACCCAGATGACCGATGTGGTTGGGGTATTTCACCTCGCGGTGCAGGATATACCTTTGGCCAGGATATATCTGAACAATTCAATCATACAAATAGTTTAAAGTTGATTGCAAGAGCTCATCAATTGGTTATGGATGGATTCAACTGGGCCCATGAACAAAAGGTGGTTACCATATTCAGTGCACCTAATTATTGCTATCGCTGTGGGAACATGGCGTCTGTTTTAGAAGTTGATGACTGCAGGGGCCATACTTTCATCCAGTTTGAGCCTGCTCCTAGGAGAGGGGAACCGGATGTCACTCGTAGAACACCTGATTACTTTTTATGA

Protein Analysis

270

Amino Acids

30.97

Weight (kDa)

5.33

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 12 - 205 1.2e-46 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 309
AccII CGCG 1 cut(s) 525
AciI CCGC 3 cut(s) 80, 452, 525
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 1 cut(s) 427
AcyI GRCGYC 1 cut(s) 701
AfiI CCNNNNNNNGG 3 cut(s) 309, 458, 767
AgsI TTSAA 5 cut(s) 167, 402, 443, 568, 628
AjnI CCWGG 1 cut(s) 546
AluBI AGCT 2 cut(s) 85, 602
AluI AGCT 2 cut(s) 85, 602
Alw21I GWGCWC 3 cut(s) 87, 604, 670
Alw44I GTGCAC 1 cut(s) 666
AoxI GGCC 5 cut(s) 186, 460, 544, 633, 730
ApaI GGGCCC 1 cut(s) 637
ApaLI GTGCAC 1 cut(s) 666
ApoI RAATTY 1 cut(s) 427
Asp700I GAANNNNTTC 2 cut(s) 282, 317
AspA2I CCTAGG 1 cut(s) 761
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 6 cut(s) 145, 187, 461, 633, 634, 730
AsuHPI GGTGA 3 cut(s) 313, 387, 510
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 145
AvrII CCTAGG 1 cut(s) 761
BaeGI GKGCMC 2 cut(s) 637, 670
BalI TGGCCA 1 cut(s) 546
BanII GRGCYC 3 cut(s) 87, 604, 637
BbsI GAAGAC 2 cut(s) 275, 320
Bbv12I GWGCWC 3 cut(s) 87, 604, 670
BccI CCATC 1 cut(s) 614
BciT130I CCWGG 1 cut(s) 548
BfaI CTAG 2 cut(s) 182, 762
BfmI CTRYAG 1 cut(s) 724
BlnI CCTAGG 1 cut(s) 761
Bme1390I CCNGG 1 cut(s) 548
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 6 cut(s) 145, 187, 461, 633, 634, 730
BmiI GGNNCC 5 cut(s) 450, 463, 635, 731, 774
BmrFI CCNGG 1 cut(s) 548
BmrI ACTGGG 2 cut(s) 29, 640
BmsI GCATC 1 cut(s) 222
BmuI ACTGGG 2 cut(s) 29, 640
BpiI GAAGAC 2 cut(s) 275, 320
Bpu14I TTCGAA 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 701
BsaJI CCNNGG 2 cut(s) 148, 761
BsaWI WCCGGW 1 cut(s) 775
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 458, 767
Bse1I ACTGG 4 cut(s) 35, 303, 635, 745
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 1 cut(s) 548
BseDI CCNNGG 2 cut(s) 148, 761
BseGI GGATG 3 cut(s) 625, 741, 784
BseLI CCNNNNNNNGG 3 cut(s) 309, 458, 767
BseMI GCAATG 1 cut(s) 19
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 4 cut(s) 35, 303, 635, 745
BseSI GKGCMC 2 cut(s) 637, 670
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 5 cut(s) 188, 462, 546, 635, 732
BsiHKAI GWGCWC 3 cut(s) 87, 604, 670
BsiSI CCGG 1 cut(s) 776
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 3 cut(s) 309, 458, 767
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 5 cut(s) 188, 462, 546, 635, 732
Bsp119I TTCGAA 1 cut(s) 91
Bsp120I GGGCCC 1 cut(s) 633
Bsp1286I GDGCHC 4 cut(s) 87, 604, 637, 670
Bsp143I GATC 2 cut(s) 433, 472
BspACI CCGC 3 cut(s) 80, 452, 525
BspANI GGCC 5 cut(s) 188, 462, 546, 635, 732
BspCNI CTCAG 1 cut(s) 268
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 5 cut(s) 450, 463, 635, 731, 774
BspMAI CTGCAG 1 cut(s) 728
BspT104I TTCGAA 1 cut(s) 91
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 4 cut(s) 35, 303, 635, 745
BssECI CCNNGG 2 cut(s) 148, 761
BssMI GATC 2 cut(s) 433, 472
BssNI GRCGYC 1 cut(s) 701
BssT1I CCWWGG 1 cut(s) 761
Bst2UI CCWGG 1 cut(s) 548
Bst4CI ACNGT 2 cut(s) 149, 172
BstACI GRCGYC 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 2 cut(s) 224, 255
BstDSI CCRYGG 1 cut(s) 148
BstEII GGTNACC 1 cut(s) 652
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 3 cut(s) 625, 741, 784
BstFNI CGCG 1 cut(s) 525
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 2 cut(s) 436, 475
BstMBI GATC 2 cut(s) 433, 472
BstNI CCWGG 1 cut(s) 548
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 1 cut(s) 546
BstSFI CTRYAG 1 cut(s) 724
BstSLI GKGCMC 2 cut(s) 637, 670
BstUI CGCG 1 cut(s) 525
BstV2I GAAGAC 2 cut(s) 275, 320
BsuRI GGCC 5 cut(s) 188, 462, 546, 635, 732
BtgI CCRYGG 1 cut(s) 148
BtsCI GGATG 3 cut(s) 625, 741, 784
BtsIMutI CAGTG 4 cut(s) 42, 310, 344, 670
Cac8I GCNNGC 1 cut(s) 756
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 6 cut(s) 145, 187, 461, 633, 634, 730
CseI GACGC 1 cut(s) 690
CviAII CATG 7 cut(s) 59, 236, 383, 455, 466, 638, 697
DdeI CTNAG 2 cut(s) 224, 255
DpnI GATC 2 cut(s) 435, 474
DpnII GATC 2 cut(s) 433, 472
DraI TTTAAA 1 cut(s) 585
EaeI YGGCCR 1 cut(s) 544
Ecl136II GAGCTC 2 cut(s) 85, 602
Eco130I CCWWGG 1 cut(s) 761
Eco24I GRGCYC 3 cut(s) 87, 604, 637
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 2 cut(s) 85, 602
Eco91I GGTNACC 1 cut(s) 652
EcoICRI GAGCTC 2 cut(s) 85, 602
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 1 cut(s) 546
EcoT14I CCWWGG 1 cut(s) 761
EcoT38I GRGCYC 3 cut(s) 87, 604, 637
ErhI CCWWGG 1 cut(s) 761
FaeI CATG 7 cut(s) 62, 239, 386, 458, 469, 641, 700
FaqI GGGAC 1 cut(s) 93
FatI CATG 7 cut(s) 58, 235, 382, 454, 465, 637, 696
FokI GGATG 3 cut(s) 632, 728, 791
FriOI GRGCYC 3 cut(s) 87, 604, 637
FspBI CTAG 2 cut(s) 182, 762
GlaI GCGC 1 cut(s) 212
HaeIII GGCC 5 cut(s) 188, 462, 546, 635, 732
HapII CCGG 1 cut(s) 776
HgaI GACGC 1 cut(s) 690
HhaI GCGC 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 701
Hin1II CATG 7 cut(s) 62, 239, 386, 458, 469, 641, 700
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 2 cut(s) 362, 624
HpaII CCGG 1 cut(s) 776
HphI GGTGA 3 cut(s) 313, 387, 510
Hpy166II GTNNAC 2 cut(s) 145, 668
Hpy188I TCNGA 4 cut(s) 249, 367, 487, 559
Hpy188III TCNNGA 3 cut(s) 71, 110, 443
Hpy8I GTNNAC 2 cut(s) 145, 668
HpyAV CCTTC 3 cut(s) 328, 408, 452
HpyCH4III ACNGT 2 cut(s) 149, 172
HpyCH4V TGCA 5 cut(s) 382, 530, 596, 668, 726
HpyF3I CTNAG 2 cut(s) 224, 255
Hsp92I GRCGYC 1 cut(s) 701
Hsp92II CATG 7 cut(s) 62, 239, 386, 458, 469, 641, 700
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 433, 472
LmnI GCTCC 2 cut(s) 82, 763
LweI GCATC 1 cut(s) 222
MaeI CTAG 2 cut(s) 182, 762
MaeIII GTNAC 4 cut(s) 37, 172, 652, 781
MalI GATC 2 cut(s) 435, 474
MboI GATC 2 cut(s) 433, 472
MboII GAAGA 3 cut(s) 17, 275, 325
MfeI CAATTG 1 cut(s) 608
MhlI GDGCHC 4 cut(s) 87, 604, 637, 670
MlsI TGGCCA 1 cut(s) 546
MluCI AATT 7 cut(s) 42, 93, 118, 427, 563, 608, 673
MluNI TGGCCA 1 cut(s) 546
MlyI GAGTC 1 cut(s) 371
MnlI CCTC 5 cut(s) 92, 221, 439, 531, 761
Mox20I TGGCCA 1 cut(s) 546
MroXI GAANNNNTTC 2 cut(s) 282, 317
MscI TGGCCA 1 cut(s) 546
MseI TTAA 1 cut(s) 584
Msp20I TGGCCA 1 cut(s) 546
MspI CCGG 1 cut(s) 776
MspR9I CCNGG 1 cut(s) 548
MunI CAATTG 1 cut(s) 608
MvaI CCWGG 1 cut(s) 548
MvnI CGCG 1 cut(s) 525
NdeII GATC 2 cut(s) 433, 472
NlaIII CATG 7 cut(s) 62, 239, 386, 458, 469, 641, 700
NlaIV GGNNCC 5 cut(s) 450, 463, 635, 731, 774
NmuCI GTSAC 2 cut(s) 37, 781
NspV TTCGAA 1 cut(s) 91
PdmI GAANNNNTTC 2 cut(s) 282, 317
PfeI GAWTC 1 cut(s) 624
PflMI CCANNNNNTGG 1 cut(s) 309
PleI GAGTC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 370
Psp124BI GAGCTC 2 cut(s) 87, 604
Psp6I CCWGG 1 cut(s) 546
PspEI GGTNACC 1 cut(s) 652
PspGI CCWGG 1 cut(s) 546
PspN4I GGNNCC 5 cut(s) 450, 463, 635, 731, 774
PspOMI GGGCCC 1 cut(s) 633
PspPI GGNCC 6 cut(s) 145, 187, 461, 633, 634, 730
PstI CTGCAG 1 cut(s) 728
SacI GAGCTC 2 cut(s) 87, 604
SaqAI TTAA 1 cut(s) 584
Sau3AI GATC 2 cut(s) 433, 472
Sau96I GGNCC 6 cut(s) 145, 187, 461, 633, 634, 730
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 548
SduI GDGCHC 4 cut(s) 87, 604, 637, 670
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 724
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 1 cut(s) 145
Sse9I AATT 7 cut(s) 42, 93, 118, 427, 563, 608, 673
SsiI CCGC 3 cut(s) 80, 452, 525
SspMI CTAG 2 cut(s) 182, 762
SstI GAGCTC 2 cut(s) 87, 604
StyD4I CCNGG 1 cut(s) 546
StyI CCWWGG 1 cut(s) 761
TaaI ACNGT 2 cut(s) 149, 172
TaqI TCGA 2 cut(s) 91, 405
TaqII GACCGA 1 cut(s) 513
TasI AATT 7 cut(s) 42, 93, 118, 427, 563, 608, 673
TfiI GAWTC 1 cut(s) 624
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 4 cut(s) 42, 310, 351, 670
TseFI GTSAC 2 cut(s) 37, 781
Tsp45I GTSAC 2 cut(s) 37, 781
TspDTI ATGAA 6 cut(s) 47, 252, 291, 471, 654, 730
TspRI CASTG 4 cut(s) 42, 310, 351, 670
Van91I CCANNNNNTGG 1 cut(s) 309
VneI GTGCAC 1 cut(s) 666
VpaK11BI GGWCC 1 cut(s) 145
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 1 cut(s) 427
XmaJI CCTAGG 1 cut(s) 761
XmnI GAANNNNTTC 2 cut(s) 282, 317
XspI CTAG 2 cut(s) 182, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.