FvH4_4g10820

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003798
Physical Location & Seq
Reverse (-)
14530207 .. 14531136
930 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10820.t1

Sequence Viewer

Length: 930 bp
ATGTCAAGGCCTTCGTTGAATCAACGAAATAACAAGTCCAGGTCGTGTAAGAGACGAGAATTTGATGCTAATAATATGAATGAAGATAACAAAGTAGTGTTGACAAGGAAAGTCCGGATAATGTTTAACGACCCTGATGCTACTGATTCTTCTTCATCCTCGAGCGAACAAGATGAAACGATGGCCAATAAGAAACCGTCGAAATCGAAAAGGTTTGTACGACAAGTCCTCATTCATATGCATGATTCAGATTCAGGTGGTAAGTGGGCAAAGACTCGAAAGCGACCGTCGTCGTCGTCGCCTTTCAGAGGTGTAAGGCTAAGAAAATATGGTAAATGGGCGGCTGAGATTCGAAATCCTGTCAAAGGTGGCCGACTTTGGTTGGGGACGTACGTCACTGCCGAGGAAGCTTCTCAGGCGTACGAGACCGCGAGGTCTAAATTTGATGAAATTCTCAGCAGGGCAGCAGCCGCTTCAACTAATAATAACTGCAGTGTCGACAATATCAACAACACTAGTAGTAGTAGTAGTAATGCAAACCAAGAAGACCTATTTCTTTTAAGAAAAGCAACTGATGATCCAATAATGTCGTCTCCGGTTTCGGTTCTGGATCCGGCAATGTCGACGGATTCCATCAGCGTTCACAATGTTGGACAACTGCAAGTATCATTGGACTCGAGTTTCCAGCTGAATGAAGCATTGGAGTCTCTCCACATTGATCACCAGCAGCAGCAAGACGTCTTCAACTTTGTACGACATGAGGAGCTTAACCAACTTATCTCCTCCATTGCCTCCTCCTCCTCCTTGGAGGAACTTGGAAAGCATTTAGACGGAACCTATTGTAGCATTGATGATATAAAGATTGGTGGATGTGATGATCATGGACACTACACCAGTCTTCCTGATTGTGACTTTGACGATTTATATTAA

Protein Analysis

310

Amino Acids

34.52

Weight (kDa)

6.01

Isoelectric Point (pI)

70.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 102 - 149 1.4e-08 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AatII GACGTC 1 cut(s) 741
AccI GTMKAC 2 cut(s) 498, 623
AccII CGCG 1 cut(s) 431
AccIII TCCGGA 1 cut(s) 114
AciI CCGC 3 cut(s) 341, 429, 471
AclWI GGATC 3 cut(s) 572, 605, 618
AcoI YGGCCR 2 cut(s) 183, 370
AcsI RAATTY 3 cut(s) 59, 440, 450
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 4 cut(s) 219, 392, 422, 753
AfiI CCNNNNNNNGG 2 cut(s) 308, 365
AgsI TTSAA 3 cut(s) 19, 477, 745
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 1 cut(s) 38
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AluBI AGCT 3 cut(s) 410, 688, 766
AluI AGCT 3 cut(s) 410, 688, 766
Alw26I GTCTC 4 cut(s) 46, 419, 597, 711
AlwI GGATC 3 cut(s) 572, 605, 618
Ama87I CYCGRG 2 cut(s) 160, 676
Aor13HI TCCGGA 1 cut(s) 114
AoxI GGCC 3 cut(s) 8, 183, 370
ApeKI GCWGC 4 cut(s) 464, 467, 727, 730
ApoI RAATTY 3 cut(s) 59, 440, 450
AsuHPI GGTGA 1 cut(s) 713
AsuII TTCGAA 1 cut(s) 352
AvaI CYCGRG 2 cut(s) 160, 676
BalI TGGCCA 1 cut(s) 185
BamHI GGATCC 1 cut(s) 610
BbsI GAAGAC 3 cut(s) 552, 733, 890
BbvI GCAGC 4 cut(s) 476, 479, 739, 742
BccI CCATC 2 cut(s) 175, 641
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 1 cut(s) 40
BclI TGATCA 2 cut(s) 718, 877
BcoDI GTCTC 4 cut(s) 46, 419, 597, 711
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 490
BisI GCNGC 6 cut(s) 342, 465, 468, 471, 728, 731
BlsI GCNGC 6 cut(s) 343, 466, 469, 472, 729, 732
Bme1390I CCNGG 1 cut(s) 40
BmeT110I CYCGRG 2 cut(s) 160, 676
BmiI GGNNCC 2 cut(s) 612, 835
BmrFI CCNGG 1 cut(s) 40
BmsI GCATC 2 cut(s) 55, 127
BoxI GACNNNNGTC 2 cut(s) 289, 392
BpiI GAAGAC 3 cut(s) 552, 733, 890
Bpu14I TTCGAA 1 cut(s) 352
BsaHI GRCGYC 1 cut(s) 738
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 402, 804
BsaWI WCCGGW 2 cut(s) 114, 595
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 365
Bse1I ACTGG 1 cut(s) 894
Bse3DI GCAATG 2 cut(s) 624, 786
BseAI TCCGGA 1 cut(s) 114
BseBI CCWGG 1 cut(s) 40
BseDI CCNNGG 2 cut(s) 402, 804
BseGI GGATG 2 cut(s) 155, 875
BseLI CCNNNNNNNGG 2 cut(s) 308, 365
BseMI GCAATG 2 cut(s) 624, 786
BseMII CTCAG 3 cut(s) 336, 428, 469
BseNI ACTGG 1 cut(s) 894
BseRI GAGGAG 5 cut(s) 772, 776, 784, 787, 790
BseXI GCAGC 4 cut(s) 476, 479, 739, 742
Bsh1236I CGCG 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 287
BshFI GGCC 3 cut(s) 10, 185, 372
BsiEI CGRYCG 1 cut(s) 287
BsiHKCI CYCGRG 2 cut(s) 160, 676
BsiSI CCGG 3 cut(s) 115, 596, 614
BsiWI CGTACG 2 cut(s) 390, 420
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 308, 365
BsmAI GTCTC 4 cut(s) 46, 419, 597, 711
BsmBI CGTCTC 2 cut(s) 46, 597
BsmFI GGGAC 1 cut(s) 400
BsnI GGCC 3 cut(s) 10, 185, 372
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 2 cut(s) 160, 676
Bsp119I TTCGAA 1 cut(s) 352
Bsp13I TCCGGA 1 cut(s) 114
Bsp143I GATC 4 cut(s) 577, 610, 718, 877
BspACI CCGC 3 cut(s) 341, 429, 471
BspANI GGCC 3 cut(s) 10, 185, 372
BspCNI CTCAG 3 cut(s) 337, 427, 468
BspEI TCCGGA 1 cut(s) 114
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 612, 835
BspMAI CTGCAG 1 cut(s) 494
BspPI GGATC 3 cut(s) 572, 605, 618
BspT104I TTCGAA 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 2 cut(s) 624, 786
BsrI ACTGG 1 cut(s) 894
BssECI CCNNGG 2 cut(s) 402, 804
BssMI GATC 4 cut(s) 577, 610, 718, 877
BssNI GRCGYC 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 804
Bst2UI CCWGG 1 cut(s) 40
Bst4CI ACNGT 2 cut(s) 198, 288
BstACI GRCGYC 1 cut(s) 738
BstBI TTCGAA 1 cut(s) 352
BstDEI CTNAG 4 cut(s) 320, 345, 414, 455
BstENI CCTNNNNNAGG 2 cut(s) 306, 363
BstF5I GGATG 2 cut(s) 155, 875
BstFNI CGCG 1 cut(s) 431
BstKTI GATC 4 cut(s) 580, 613, 721, 880
BstMAI GTCTC 4 cut(s) 46, 419, 597, 711
BstMBI GATC 4 cut(s) 577, 610, 718, 877
BstMCI CGRYCG 1 cut(s) 287
BstMWI GCNNNNNNNGC 3 cut(s) 407, 416, 470
BstNI CCWGG 1 cut(s) 40
BstPAI GACNNNNGTC 2 cut(s) 289, 392
BstSCI CCNGG 1 cut(s) 38
BstSFI CTRYAG 1 cut(s) 490
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 4 cut(s) 476, 479, 739, 742
BstV2I GAAGAC 3 cut(s) 552, 733, 890
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 3 cut(s) 10, 185, 372
BtsCI GGATG 2 cut(s) 155, 875
BtsI GCAGTG 2 cut(s) 396, 499
BtsIMutI CAGTG 2 cut(s) 396, 499
Csp6I GTAC 4 cut(s) 218, 391, 421, 752
CviAII CATG 3 cut(s) 242, 758, 881
CviJI RGCY 9 cut(s) 10, 185, 319, 344, 372, 410, 470, 688, 766
CviKI_1 RGCY 9 cut(s) 10, 185, 319, 344, 372, 410, 470, 688, 766
CviQI GTAC 4 cut(s) 218, 391, 421, 752
DdeI CTNAG 4 cut(s) 320, 345, 414, 455
DpnI GATC 4 cut(s) 579, 612, 720, 879
DpnII GATC 4 cut(s) 577, 610, 718, 877
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 2 cut(s) 183, 370
Eco130I CCWWGG 1 cut(s) 804
Eco147I AGGCCT 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 419
Eco88I CYCGRG 2 cut(s) 160, 676
EcoNI CCTNNNNNAGG 2 cut(s) 306, 363
EcoRII CCWGG 1 cut(s) 38
EcoT14I CCWWGG 1 cut(s) 804
EcoT22I ATGCAT 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 804
Esp3I CGTCTC 2 cut(s) 46, 597
FaeI CATG 3 cut(s) 245, 761, 884
FaiI YATR 9 cut(s) 77, 237, 239, 243, 330, 759, 857, 882, 925
FaqI GGGAC 1 cut(s) 400
FatI CATG 3 cut(s) 241, 757, 880
FauNDI CATATG 1 cut(s) 237
FbaI TGATCA 2 cut(s) 718, 877
FblI GTMKAC 2 cut(s) 498, 623
Fnu4HI GCNGC 6 cut(s) 342, 465, 468, 471, 728, 731
FokI GGATG 2 cut(s) 142, 882
Fsp4HI GCNGC 6 cut(s) 342, 465, 468, 471, 728, 731
FspBI CTAG 1 cut(s) 516
GluI GCNGC 6 cut(s) 342, 465, 468, 471, 728, 731
HaeIII GGCC 3 cut(s) 10, 185, 372
HapII CCGG 3 cut(s) 115, 596, 614
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 3 cut(s) 245, 761, 884
HincII GTYRAC 3 cut(s) 102, 499, 624
HindII GTYRAC 3 cut(s) 102, 499, 624
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 9 cut(s) 19, 146, 245, 251, 274, 349, 629, 674, 704
HpaII CCGG 3 cut(s) 115, 596, 614
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 4 cut(s) 102, 499, 624, 643
Hpy188I TCNGA 2 cut(s) 250, 308
Hpy188III TCNNGA 3 cut(s) 115, 608, 902
Hpy8I GTNNAC 4 cut(s) 102, 499, 624, 643
Hpy99I CGWCG 6 cut(s) 202, 292, 295, 298, 301, 628
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 2 cut(s) 198, 288
HpyCH4IV ACGT 3 cut(s) 389, 393, 738
HpyCH4V TGCA 4 cut(s) 241, 492, 536, 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 416, 470
HpyF3I CTNAG 4 cut(s) 320, 345, 414, 455
HpySE526I ACGT 3 cut(s) 389, 393, 738
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 3 cut(s) 245, 761, 884
Kpn2I TCCGGA 1 cut(s) 114
Ksp22I TGATCA 2 cut(s) 718, 877
Kzo9I GATC 4 cut(s) 577, 610, 718, 877
LmnI GCTCC 1 cut(s) 763
Lsp1109I GCAGC 4 cut(s) 476, 479, 739, 742
LweI GCATC 2 cut(s) 55, 127
MaeI CTAG 1 cut(s) 516
MaeII ACGT 3 cut(s) 389, 393, 738
MaeIII GTNAC 2 cut(s) 394, 908
MalI GATC 4 cut(s) 579, 612, 720, 879
MboI GATC 4 cut(s) 577, 610, 718, 877
MboII GAAGA 6 cut(s) 95, 141, 144, 557, 733, 890
MflI RGATCY 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 3 cut(s) 59, 440, 450
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 3 cut(s) 268, 668, 713
MmeI TCCRAC 1 cut(s) 631
Mox20I TGGCCA 1 cut(s) 185
Mph1103I ATGCAT 1 cut(s) 243
MroI TCCGGA 1 cut(s) 114
MscI TGGCCA 1 cut(s) 185
MseI TTAA 4 cut(s) 126, 560, 768, 928
MslI CAYNNNNRTG 2 cut(s) 236, 240
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 3 cut(s) 115, 596, 614
MspR9I CCNGG 1 cut(s) 40
MvaI CCWGG 1 cut(s) 40
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 3 cut(s) 407, 416, 470
NdeI CATATG 1 cut(s) 237
NdeII GATC 4 cut(s) 577, 610, 718, 877
NlaIII CATG 3 cut(s) 245, 761, 884
NlaIV GGNNCC 2 cut(s) 612, 835
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 394, 908
NsiI ATGCAT 1 cut(s) 243
NspV TTCGAA 1 cut(s) 352
PaeR7I CTCGAG 2 cut(s) 160, 676
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PfeI GAWTC 6 cut(s) 19, 146, 245, 251, 349, 629
Pfl23II CGTACG 2 cut(s) 390, 420
PkrI GCNGC 6 cut(s) 343, 466, 469, 472, 729, 732
PleI GAGTC 3 cut(s) 268, 668, 712
PpsI GAGTC 3 cut(s) 268, 668, 712
PshAI GACNNNNGTC 2 cut(s) 289, 392
Psp6I CCWGG 1 cut(s) 38
PspGI CCWGG 1 cut(s) 38
PspLI CGTACG 2 cut(s) 390, 420
PspN4I GGNNCC 2 cut(s) 612, 835
PspXI VCTCGAGB 2 cut(s) 160, 676
PstI CTGCAG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 4 cut(s) 219, 392, 422, 753
RsaNI GTAC 4 cut(s) 218, 391, 421, 752
RseI CAYNNNNRTG 2 cut(s) 236, 240
SalI GTCGAC 2 cut(s) 497, 622
SaqAI TTAA 4 cut(s) 126, 560, 768, 928
SatI GCNGC 6 cut(s) 342, 465, 468, 471, 728, 731
Sau3AI GATC 4 cut(s) 577, 610, 718, 877
SchI GAGTC 3 cut(s) 268, 668, 713
ScrFI CCNGG 1 cut(s) 40
SfaNI GCATC 2 cut(s) 55, 127
SfcI CTRYAG 1 cut(s) 490
Sfr274I CTCGAG 2 cut(s) 160, 676
SfuI TTCGAA 1 cut(s) 352
SlaI CTCGAG 2 cut(s) 160, 676
SmiMI CAYNNNNRTG 2 cut(s) 236, 240
SmlI CTYRAG 2 cut(s) 160, 676
SmoI CTYRAG 2 cut(s) 160, 676
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 3 cut(s) 59, 440, 450
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 3 cut(s) 341, 429, 471
SspMI CTAG 1 cut(s) 516
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 1 cut(s) 804
TaaI ACNGT 2 cut(s) 198, 288
TaiI ACGT 3 cut(s) 392, 396, 741
TaqI TCGA 8 cut(s) 161, 200, 206, 277, 352, 498, 623, 677
TasI AATT 3 cut(s) 59, 440, 450
TauI GCSGC 2 cut(s) 344, 473
TfiI GAWTC 6 cut(s) 19, 146, 245, 251, 349, 629
Tru1I TTAA 4 cut(s) 126, 560, 768, 928
Tru9I TTAA 4 cut(s) 126, 560, 768, 928
TscAI CASTG 2 cut(s) 403, 499
TseFI GTSAC 2 cut(s) 394, 908
TseI GCWGC 4 cut(s) 464, 467, 727, 730
Tsp45I GTSAC 2 cut(s) 394, 908
TspDTI ATGAA 7 cut(s) 92, 96, 144, 189, 224, 462, 708
TspGWI ACGGA 2 cut(s) 641, 846
TspRI CASTG 2 cut(s) 403, 499
XagI CCTNNNNNAGG 2 cut(s) 306, 363
XapI RAATTY 3 cut(s) 59, 440, 450
XhoI CTCGAG 2 cut(s) 160, 676
XmiI GTMKAC 2 cut(s) 498, 623
XspI CTAG 1 cut(s) 516
ZraI GACGTC 1 cut(s) 739
Zsp2I ATGCAT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.