RchiOBHm_Chr4g0435261

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003798
Physical Location & Seq
Reverse (-)
58888206 .. 58890377
2172 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40369

Sequence Viewer

Length: 1044 bp
ATGCCGGAGCCTCGTAATCCACGGCTGAATCAACGGAATAGTTTCAAGAAGTCTAAGAGGGAACAGCTGTTTCCTGCTGAAGAGCCCAAGATGGTGAGGAAGGTTCGGATAATCTGCTCCGATCCTGATGCTACTGATGACTCATCTTCCGAAGATGAAGGAATTGATAGAAAACTTCACAAACGCATGGTGAAGGAAATCACTCTTCCGTTTAATTTCACGCGCCCATCTAAGCTTGTTGTTGAGCCCGAGGGTTCTTCCAACAATGGAGTCAAAACCCCAACGCCTAAACCCAAGTTGGGGGTTTTGGCTAAAACCTCGAAAAAATCATCCAATTCGCCCTTTCGAGGTGTGAGGCAAAGAAAATGGGGCAAATGGGCGGCTGAAATTCGTGACCCTTTCAGCAAGACTAGGGTCTGGTTGGGAACCTACAACACTGCTGAGGATGCTTCCAAGGCCTACGAGGAAAGGAGGCTCTACTTCGAAAAGATGCTCTCTTTGGCCACAGCTTCAGAGGTCAGCTCTGAGAAAAGCAACACCACCACCACCTCTTGTTCTGCCGTCGTCTCTCGGTCGGTCAACAACACCCAACAACCTGCTGTTTCATCCGAGGAGGAATCTGACTGCGTCTACTCCCAAAGGTCTCCGGCTTCAGTTCTGGATGTTGAGACCTGTGCTTCCAACAACATTCGCAGTGGTGGTGATTTGGAGAATGGTGGTGATATGGAGACTGAAGATGTTGGGACTAATGCCCTCGGGGAGCTGCCAATTCCCAACGACTTGGGATTCATGGACGAGCAGCCATTTGGGCCGGAGCTCAACTTTGATGGGATTGAACCAGTAAGCTTTCAAGCGGAGATCGACTCGCTTCTTCTGGATGAAATTGGGCAGTTTTTCAACGATTTTGGTAGCATTGATGATGTCCATATTGGTGGCTTTGAAGGAAATGAGCCCAGCAATCTTCCTGATTGTGACTTTGGGGATCTTGGCAAGGAGGACTTTGCTTGCTGGATGACTGAAACCTCCAACATACAATGCCAATAA

Protein Analysis

347

Amino Acids

38.5

Weight (kDa)

4.75

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 115 - 163 1.1e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 630
AccII CGCG 1 cut(s) 223
AciI CCGC 2 cut(s) 380, 854
AclWI GGATC 2 cut(s) 116, 990
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 4 cut(s) 99, 495, 636, 753
AfiI CCNNNNNNNGG 3 cut(s) 299, 300, 347
AgsI TTSAA 5 cut(s) 46, 836, 851, 898, 941
AluBI AGCT 7 cut(s) 67, 235, 509, 522, 763, 817, 846
AluI AGCT 7 cut(s) 67, 235, 509, 522, 763, 817, 846
Alw21I GWGCWC 1 cut(s) 819
Alw26I GTCTC 4 cut(s) 571, 648, 662, 722
AlwI GGATC 2 cut(s) 116, 990
Ama87I CYCGRG 2 cut(s) 248, 755
AoxI GGCC 3 cut(s) 456, 501, 809
ApeKI GCWGC 2 cut(s) 763, 799
ApoI RAATTY 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 809
AsuHPI GGTGA 4 cut(s) 106, 202, 713, 731
AsuII TTCGAA 1 cut(s) 483
AvaI CYCGRG 2 cut(s) 248, 755
BalI TGGCCA 1 cut(s) 503
BanII GRGCYC 4 cut(s) 87, 249, 819, 954
Bbv12I GWGCWC 1 cut(s) 819
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 750, 811
BccI CCATC 3 cut(s) 85, 235, 821
BceAI ACGGC 2 cut(s) 38, 545
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BcoDI GTCTC 4 cut(s) 571, 648, 662, 722
BfaI CTAG 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 808
BisI GCNGC 3 cut(s) 381, 764, 800
BlsI GCNGC 3 cut(s) 382, 765, 801
BmeT110I CYCGRG 2 cut(s) 248, 755
BmgT120I GGNCC 1 cut(s) 809
BmiI GGNNCC 2 cut(s) 9, 427
BmsI GCATC 3 cut(s) 118, 436, 480
BoxI GACNNNNGTC 1 cut(s) 413
BplI GAGNNNNNCTC 4 cut(s) 506, 538, 848, 880
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 483
BsaI GGTCTC 2 cut(s) 648, 662
BsaJI CCNNGG 5 cut(s) 20, 249, 453, 609, 754
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 300, 347
Bse1I ACTGG 1 cut(s) 839
BseDI CCNNGG 5 cut(s) 20, 249, 453, 609, 754
BseGI GGATG 6 cut(s) 329, 451, 605, 667, 883, 1017
BseLI CCNNNNNNNGG 3 cut(s) 299, 300, 347
BseMII CTCAG 2 cut(s) 432, 516
BseNI ACTGG 1 cut(s) 839
BseRI GAGGAG 1 cut(s) 626
BseXI GCAGC 2 cut(s) 750, 811
BseYI CCCAGC 1 cut(s) 953
Bsh1236I CGCG 1 cut(s) 223
Bsh1285I CGRYCG 1 cut(s) 575
BshFI GGCC 3 cut(s) 458, 503, 811
BsiEI CGRYCG 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 819
BsiHKCI CYCGRG 2 cut(s) 248, 755
BsiSI CCGG 3 cut(s) 5, 647, 812
BslFI GGGAC 1 cut(s) 757
BslI CCNNNNNNNGG 3 cut(s) 299, 300, 347
BsmAI GTCTC 4 cut(s) 571, 648, 662, 722
BsmBI CGTCTC 1 cut(s) 571
BsmFI GGGAC 1 cut(s) 757
BsnI GGCC 3 cut(s) 458, 503, 811
Bso31I GGTCTC 2 cut(s) 648, 662
BsoBI CYCGRG 2 cut(s) 248, 755
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 4 cut(s) 87, 249, 819, 954
Bsp143I GATC 3 cut(s) 121, 858, 982
BspACI CCGC 2 cut(s) 380, 854
BspANI GGCC 3 cut(s) 458, 503, 811
BspCNI CTCAG 2 cut(s) 433, 517
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 9, 427
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 2 cut(s) 116, 990
BspQI GCTCTTC 1 cut(s) 75
BspT104I TTCGAA 1 cut(s) 483
BspTNI GGTCTC 2 cut(s) 648, 662
BsrI ACTGG 1 cut(s) 839
BssECI CCNNGG 5 cut(s) 20, 249, 453, 609, 754
BssMI GATC 3 cut(s) 121, 858, 982
BssT1I CCWWGG 1 cut(s) 453
Bst6I CTCTTC 2 cut(s) 75, 210
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 1006
BstDEI CTNAG 4 cut(s) 54, 231, 441, 525
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 6 cut(s) 329, 451, 605, 667, 883, 1017
BstFNI CGCG 1 cut(s) 223
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 3 cut(s) 124, 861, 985
BstMAI GTCTC 4 cut(s) 571, 648, 662, 722
BstMBI GATC 3 cut(s) 121, 858, 982
BstMCI CGRYCG 1 cut(s) 575
BstMWI GCNNNNNNNGC 3 cut(s) 446, 455, 808
BstPAI GACNNNNGTC 1 cut(s) 413
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 750, 811
BstX2I RGATCY 1 cut(s) 982
BstXI CCANNNNNNTGG 2 cut(s) 781, 932
BstYI RGATCY 1 cut(s) 982
BsuRI GGCC 3 cut(s) 458, 503, 811
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 6 cut(s) 329, 451, 605, 667, 883, 1017
BtsI GCAGTG 2 cut(s) 435, 700
BtsIMutI CAGTG 2 cut(s) 435, 700
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 1006
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 809
CseI GACGC 1 cut(s) 616
CviAII CATG 2 cut(s) 187, 790
DdeI CTNAG 4 cut(s) 54, 231, 441, 525
DpnI GATC 3 cut(s) 123, 860, 984
DpnII GATC 3 cut(s) 121, 858, 982
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 2 cut(s) 75, 210
EarI CTCTTC 2 cut(s) 75, 210
Ecl136II GAGCTC 1 cut(s) 817
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 4 cut(s) 87, 249, 819, 954
Eco31I GGTCTC 2 cut(s) 648, 662
Eco53kI GAGCTC 1 cut(s) 817
Eco57I CTGAAG 4 cut(s) 99, 495, 636, 753
Eco88I CYCGRG 2 cut(s) 248, 755
EcoICRI GAGCTC 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 4 cut(s) 87, 249, 819, 954
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 571
FaeI CATG 2 cut(s) 190, 793
FaiI YATR 5 cut(s) 188, 725, 791, 927, 1031
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FaqI GGGAC 1 cut(s) 757
FatI CATG 2 cut(s) 186, 789
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 3 cut(s) 381, 764, 800
FokI GGATG 6 cut(s) 316, 458, 592, 674, 890, 1024
FriOI GRGCYC 4 cut(s) 87, 249, 819, 954
Fsp4HI GCNGC 3 cut(s) 381, 764, 800
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 224
GluI GCNGC 3 cut(s) 381, 764, 800
GsaI CCCAGC 1 cut(s) 957
HaeIII GGCC 3 cut(s) 458, 503, 811
HapII CCGG 3 cut(s) 5, 647, 812
HgaI GACGC 1 cut(s) 616
HhaI GCGC 1 cut(s) 225
Hin1II CATG 2 cut(s) 190, 793
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 2 cut(s) 233, 844
HinfI GANTC 6 cut(s) 28, 140, 270, 617, 786, 863
HpaII CCGG 3 cut(s) 5, 647, 812
HphI GGTGA 4 cut(s) 106, 202, 713, 731
Hpy166II GTNNAC 2 cut(s) 580, 631
Hpy188I TCNGA 7 cut(s) 108, 121, 151, 514, 526, 610, 622
Hpy188III TCNNGA 6 cut(s) 46, 125, 392, 659, 875, 965
Hpy8I GTNNAC 2 cut(s) 580, 631
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 4 cut(s) 94, 152, 187, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 455, 808
HpyF3I CTNAG 4 cut(s) 54, 231, 441, 525
Hsp92II CATG 2 cut(s) 190, 793
HspAI GCGC 1 cut(s) 223
Kzo9I GATC 3 cut(s) 121, 858, 982
LguI GCTCTTC 1 cut(s) 75
LmnI GCTCC 4 cut(s) 7, 122, 760, 814
Lsp1109I GCAGC 2 cut(s) 750, 811
LweI GCATC 3 cut(s) 118, 436, 480
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 392, 971
MalI GATC 3 cut(s) 123, 860, 984
MboI GATC 3 cut(s) 121, 858, 982
MboII GAAGA 8 cut(s) 92, 138, 164, 197, 249, 746, 863, 953
MflI RGATCY 1 cut(s) 982
MhlI GDGCHC 4 cut(s) 87, 249, 819, 954
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 6 cut(s) 162, 214, 334, 387, 768, 882
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 3 cut(s) 134, 279, 857
MmeI TCCRAC 2 cut(s) 285, 705
Mox20I TGGCCA 1 cut(s) 503
MroXI GAANNNNTTC 1 cut(s) 41
MscI TGGCCA 1 cut(s) 503
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 930
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 3 cut(s) 5, 647, 812
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 3 cut(s) 446, 455, 808
NdeII GATC 3 cut(s) 121, 858, 982
NlaIII CATG 2 cut(s) 190, 793
NlaIV GGNNCC 2 cut(s) 9, 427
NmuCI GTSAC 2 cut(s) 392, 971
NspV TTCGAA 1 cut(s) 483
PceI AGGCCT 1 cut(s) 458
PciSI GCTCTTC 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 3 cut(s) 28, 617, 786
PflFI GACNNNGTC 1 cut(s) 626
PkrI GCNGC 3 cut(s) 382, 765, 801
PleI GAGTC 3 cut(s) 134, 278, 857
PpsI GAGTC 3 cut(s) 134, 278, 857
PshAI GACNNNNGTC 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 819
PspFI CCCAGC 1 cut(s) 953
PspN4I GGNNCC 2 cut(s) 9, 427
PspPI GGNCC 1 cut(s) 809
PsuI RGATCY 1 cut(s) 982
PsyI GACNNNGTC 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 930
SacI GAGCTC 1 cut(s) 819
SapI GCTCTTC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 3 cut(s) 381, 764, 800
Sau3AI GATC 3 cut(s) 121, 858, 982
Sau96I GGNCC 1 cut(s) 809
SchI GAGTC 3 cut(s) 134, 279, 857
SduI GDGCHC 4 cut(s) 87, 249, 819, 954
SfaNI GCATC 3 cut(s) 118, 436, 480
SfuI TTCGAA 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 930
Sse9I AATT 6 cut(s) 162, 214, 334, 387, 768, 882
SseBI AGGCCT 1 cut(s) 458
SsiI CCGC 2 cut(s) 380, 854
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 819
StuI AGGCCT 1 cut(s) 458
StyI CCWWGG 1 cut(s) 453
TaqI TCGA 4 cut(s) 320, 346, 483, 861
TaqII GACCGA 2 cut(s) 561, 565
TasI AATT 6 cut(s) 162, 214, 334, 387, 768, 882
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 3 cut(s) 28, 617, 786
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 2 cut(s) 442, 700
TseFI GTSAC 2 cut(s) 392, 971
TseI GCWGC 2 cut(s) 763, 799
Tsp45I GTSAC 2 cut(s) 392, 971
TspDTI ATGAA 4 cut(s) 171, 594, 778, 894
TspGWI ACGGA 2 cut(s) 49, 198
TspRI CASTG 2 cut(s) 442, 700
Tth111I GACNNNGTC 1 cut(s) 626
XapI RAATTY 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 630
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.