Rh4AG140100

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003798
Physical Location & Seq
Forward (+)
35580874 .. 35587487
6614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG140100.1

Sequence Viewer

Length: 1164 bp
ATGTGGAGGCCGGCGCCACTAACCGTTGGACATCCTCAACGGCTACTTTCCTCGACGTGGACACCCTACTTTGGAAACTATTTGCCTTCTTCCCGGCTTTGGTTCCTAATGTGTTTCTGTATTTTGGAGAAGGAATCTACACTTGATCGAATTAGTCTAAAAACAATGTCAAGGCCTTCATTGAATCAACGAAAACAAAAGTCCAAGCAGTCGTCTAAGAGACGAGAATTTCATGCTCATAATGAAGATAACAAAGTGTTGACAAGAAAAGTCCGGATAATGTTTAACGACCCCGATGCTACTGATTCTTCATCCTCGAGCGAAGATGAAACGATGGAGACTGCACAGTATGAGAAGAAAAAATCATTAAAAAAATCAAAAAGGTTTGTACAGGAAGTCATTCATTCTTTCTTCATGCATTCAGGTAGGGCAGTTGGTTGTGAAGCCCCACCAGAGACAAGAACGACTAGAACTAGCTCAGGAAGAAAGCGATTCTCGTCTTCACCTTTCAGAGGTGTAAGGCAAAGAAAATATGGTAAATGGGCTGCTGAGATTCGAGATCCTATCAAAGGTGGCCGACTTTGGTTGGGTACGTACGCCACTGCCGAGGAAGCTTCCCAGGCTTACGAGACCATGAGGTGTAAGTTCGATGAAATTTTGAGGGCCACTACAACCAGTATTTCTGACAAAAACAACAAAGATGGCAAGAGTAATTCTGACCACGAACACCTATTTCTTTTAAGAAAAGCAACTCCAATAACAGCTTCCAACTCTGGTGGCAACGGCCTTTTGTCCCATAAGGGATCTCCGGTTGCGGTTCTTGATTTGGGAAAGAATGCGGATTCAGACAAGGAAAACATTGGCACTAATAATGTTGGACAACTGCGGATACCATTGGACTTGAGTTTCCTGGATGAAGCATTGCAGTCTCTCCCCACTGATCAGCAACAGCAAGACGTCTTCAACTTTGTACCAGATAATGAGGAGCTTGACAAACTTGCCTCCTCCATTGCCTCCTCCTCTTTAGAGGAACTTGGTAAGTATTTTGACGGAATGTATTGTAGCATTGATGATATAAAGATCGGTGGAGTTGATGATGATAACCCCACCAATCTTCCTGACTGTGACGACTTTGACGATATTCTAATAGTTCAACATAACTAG

Protein Analysis

387

Amino Acids

43.66

Weight (kDa)

7.11

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 170 - 217 1.4e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 960
AccB1I GGYRCC 1 cut(s) 13
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 3 cut(s) 815, 839, 886
AclWI GGATC 2 cut(s) 554, 811
AcoI YGGCCR 1 cut(s) 574
AcsI RAATTY 2 cut(s) 227, 654
AcyI GRCGYC 2 cut(s) 14, 957
AfaI GTAC 4 cut(s) 390, 592, 596, 972
AfiI CCNNNNNNNGG 5 cut(s) 57, 71, 99, 512, 569
AgsI TTSAA 3 cut(s) 184, 964, 1154
AjiI CACGTC 1 cut(s) 57
AjnI CCWGG 2 cut(s) 618, 909
AjuI GAANNNNNNNTTGG 2 cut(s) 748, 780
AluBI AGCT 4 cut(s) 477, 614, 764, 988
AluI AGCT 4 cut(s) 477, 614, 764, 988
Alw26I GTCTC 5 cut(s) 214, 332, 449, 623, 933
AlwI GGATC 2 cut(s) 554, 811
Ama87I CYCGRG 1 cut(s) 316
Aor13HI TCCGGA 1 cut(s) 273
AoxI GGCC 5 cut(s) 8, 173, 574, 663, 784
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 2 cut(s) 227, 654
Asp700I GAANNNNTTC 1 cut(s) 399
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 663
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 1 cut(s) 495
AvaI CYCGRG 1 cut(s) 316
BanI GGYRCC 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 492, 952
BbvI GCAGC 1 cut(s) 532
BccI CCATC 2 cut(s) 328, 695
BceAI ACGGC 2 cut(s) 56, 799
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 620, 911
BciVI GTATCC 1 cut(s) 882
BclI TGATCA 1 cut(s) 940
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 5 cut(s) 214, 332, 449, 623, 933
BfaI CTAG 3 cut(s) 468, 474, 1162
BfoI RGCGCY 1 cut(s) 17
BfuI GTATCC 1 cut(s) 882
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 3 cut(s) 94, 620, 911
BmeT110I CYCGRG 1 cut(s) 316
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 663
BmiI GGNNCC 2 cut(s) 15, 104
BmrFI CCNGG 3 cut(s) 94, 620, 911
BmsI GCATC 1 cut(s) 286
BpiI GAAGAC 2 cut(s) 492, 952
Bpu10I CCTNAGC 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 922
BpuMI CCSGG 1 cut(s) 94
BsaAI YACGTR 1 cut(s) 594
BsaHI GRCGYC 2 cut(s) 14, 957
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 2 cut(s) 606, 618
BsaWI WCCGGW 2 cut(s) 273, 808
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 71, 99, 512, 569
Bse118I RCCGGY 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 675
Bse3DI GCAATG 2 cut(s) 920, 1008
BseAI TCCGGA 1 cut(s) 273
BseBI CCWGG 2 cut(s) 620, 911
BseDI CCNNGG 2 cut(s) 606, 618
BseGI GGATG 3 cut(s) 31, 311, 919
BseLI CCNNNNNNNGG 5 cut(s) 57, 71, 99, 512, 569
BseMI GCAATG 2 cut(s) 920, 1008
BseMII CTCAG 2 cut(s) 492, 540
BseNI ACTGG 1 cut(s) 675
BseRI GAGGAG 4 cut(s) 994, 998, 1006, 1009
BseXI GCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 327
BshFI GGCC 5 cut(s) 10, 175, 576, 665, 786
BshNI GGYRCC 1 cut(s) 13
BsiHKCI CYCGRG 1 cut(s) 316
BsiSI CCGG 4 cut(s) 11, 94, 274, 809
BsiWI CGTACG 1 cut(s) 594
BslFI GGGAC 1 cut(s) 778
BslI CCNNNNNNNGG 5 cut(s) 57, 71, 99, 512, 569
BsmAI GTCTC 5 cut(s) 214, 332, 449, 623, 933
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 778
BsmI GAATGC 2 cut(s) 418, 841
BsnI GGCC 5 cut(s) 10, 175, 576, 665, 786
Bso31I GGTCTC 1 cut(s) 623
BsoBI CYCGRG 1 cut(s) 316
Bsp13I TCCGGA 1 cut(s) 273
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 5 cut(s) 145, 559, 803, 940, 1080
BspACI CCGC 3 cut(s) 815, 839, 886
BspANI GGCC 5 cut(s) 10, 175, 576, 665, 786
BspCNI CTCAG 2 cut(s) 491, 541
BspEI TCCGGA 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 15, 104
BspPI GGATC 2 cut(s) 554, 811
BspT107I GGYRCC 1 cut(s) 13
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 2 cut(s) 920, 1008
BsrFI RCCGGY 1 cut(s) 10
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 1 cut(s) 675
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 2 cut(s) 606, 618
BssMI GATC 5 cut(s) 145, 559, 803, 940, 1080
BssNI GRCGYC 2 cut(s) 14, 957
Bst2UI CCWGG 2 cut(s) 620, 911
Bst4CI ACNGT 3 cut(s) 25, 348, 1124
BstACI GRCGYC 2 cut(s) 14, 957
BstAUI TGTACA 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 3 cut(s) 216, 478, 549
BstENI CCTNNNNNAGG 2 cut(s) 510, 567
BstF5I GGATG 3 cut(s) 31, 311, 919
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 5 cut(s) 148, 562, 806, 943, 1083
BstMAI GTCTC 5 cut(s) 214, 332, 449, 623, 933
BstMBI GATC 5 cut(s) 145, 559, 803, 940, 1080
BstMWI GCNNNNNNNGC 2 cut(s) 611, 620
BstNI CCWGG 2 cut(s) 620, 911
BstSCI CCNGG 3 cut(s) 92, 618, 909
BstSNI TACGTA 1 cut(s) 594
BstV1I GCAGC 1 cut(s) 532
BstV2I GAAGAC 2 cut(s) 492, 952
BstX2I RGATCY 2 cut(s) 559, 803
BstYI RGATCY 2 cut(s) 559, 803
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 5 cut(s) 10, 175, 576, 665, 786
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 3 cut(s) 31, 311, 919
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 600, 936
Cac8I GCNNGC 1 cut(s) 12
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 663
Csp6I GTAC 4 cut(s) 389, 591, 595, 971
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 3 cut(s) 233, 415, 634
CviQI GTAC 4 cut(s) 389, 591, 595, 971
DdeI CTNAG 3 cut(s) 216, 478, 549
DinI GGCGCC 1 cut(s) 15
DpnI GATC 5 cut(s) 147, 561, 805, 942, 1082
DpnII GATC 5 cut(s) 145, 559, 803, 940, 1080
EaeI YGGCCR 1 cut(s) 574
Eco105I TACGTA 1 cut(s) 594
Eco147I AGGCCT 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 316
EcoNI CCTNNNNNAGG 2 cut(s) 510, 567
EcoRII CCWGG 2 cut(s) 618, 909
EcoT22I ATGCAT 1 cut(s) 420
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 3 cut(s) 236, 418, 637
FaiI YATR 9 cut(s) 234, 240, 351, 416, 534, 635, 798, 1076, 1158
FaqI GGGAC 1 cut(s) 778
FatI CATG 3 cut(s) 232, 414, 633
FbaI TGATCA 1 cut(s) 940
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 3 cut(s) 18, 298, 926
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 3 cut(s) 468, 474, 1162
GlaI GCGC 1 cut(s) 15
GluI GCNGC 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 5 cut(s) 10, 175, 576, 665, 786
HapII CCGG 4 cut(s) 11, 94, 274, 809
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 2 cut(s) 14, 957
Hin1II CATG 3 cut(s) 236, 418, 637
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 612
HinfI GANTC 6 cut(s) 134, 184, 305, 492, 553, 842
HpaII CCGG 4 cut(s) 11, 94, 274, 809
HphI GGTGA 1 cut(s) 495
Hpy166II GTNNAC 2 cut(s) 60, 261
Hpy188I TCNGA 4 cut(s) 512, 685, 718, 847
Hpy188III TCNNGA 5 cut(s) 274, 480, 557, 821, 1118
Hpy8I GTNNAC 2 cut(s) 60, 261
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 96, 124, 186
HpyCH4III ACNGT 3 cut(s) 25, 348, 1124
HpyCH4IV ACGT 3 cut(s) 56, 593, 957
HpyCH4V TGCA 3 cut(s) 344, 418, 925
HpyF10VI GCNNNNNNNGC 2 cut(s) 611, 620
HpyF3I CTNAG 3 cut(s) 216, 478, 549
HpySE526I ACGT 3 cut(s) 56, 593, 957
Hsp92I GRCGYC 2 cut(s) 14, 957
Hsp92II CATG 3 cut(s) 236, 418, 637
HspAI GCGC 1 cut(s) 14
KasI GGCGCC 1 cut(s) 13
Kpn2I TCCGGA 1 cut(s) 273
KroI GCCGGC 1 cut(s) 10
KroNI GCCGGC 1 cut(s) 12
Ksp22I TGATCA 1 cut(s) 940
Kzo9I GATC 5 cut(s) 145, 559, 803, 940, 1080
LmnI GCTCC 1 cut(s) 985
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 1 cut(s) 286
MaeI CTAG 3 cut(s) 468, 474, 1162
MaeII ACGT 3 cut(s) 56, 593, 957
MaeIII GTNAC 1 cut(s) 1124
MalI GATC 5 cut(s) 147, 561, 805, 942, 1082
MboI GATC 5 cut(s) 145, 559, 803, 940, 1080
MflI RGATCY 2 cut(s) 559, 803
MluCI AATT 4 cut(s) 150, 227, 654, 712
Mly113I GGCGCC 1 cut(s) 14
MmeI TCCRAC 3 cut(s) 7, 792, 856
Mph1103I ATGCAT 1 cut(s) 420
MroI TCCGGA 1 cut(s) 273
MroNI GCCGGC 1 cut(s) 10
MroXI GAANNNNTTC 1 cut(s) 399
MseI TTAA 3 cut(s) 285, 368, 740
MspI CCGG 4 cut(s) 11, 94, 274, 809
MspR9I CCNGG 3 cut(s) 94, 620, 911
Mva1269I GAATGC 2 cut(s) 418, 841
MvaI CCWGG 2 cut(s) 620, 911
MwoI GCNNNNNNNGC 2 cut(s) 611, 620
NaeI GCCGGC 1 cut(s) 12
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 1 cut(s) 94
NdeII GATC 5 cut(s) 145, 559, 803, 940, 1080
NgoMIV GCCGGC 1 cut(s) 10
NlaIII CATG 3 cut(s) 236, 418, 637
NlaIV GGNNCC 2 cut(s) 15, 104
NmeAIII GCCGAG 1 cut(s) 631
NmuCI GTSAC 1 cut(s) 1124
NsiI ATGCAT 1 cut(s) 420
PaeR7I CTCGAG 1 cut(s) 316
PceI AGGCCT 1 cut(s) 175
PcsI WCGNNNNNNNCGW 2 cut(s) 603, 1134
PctI GAATGC 2 cut(s) 418, 841
PdiI GCCGGC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 6 cut(s) 134, 184, 305, 492, 553, 842
Pfl23II CGTACG 1 cut(s) 594
PfoI TCCNGGA 1 cut(s) 909
PkrI GCNGC 1 cut(s) 547
PluTI GGCGCC 1 cut(s) 17
Ppu21I YACGTR 1 cut(s) 594
Psp6I CCWGG 2 cut(s) 618, 909
PspGI CCWGG 2 cut(s) 618, 909
PspLI CGTACG 1 cut(s) 594
PspN4I GGNNCC 2 cut(s) 15, 104
PspPI GGNCC 1 cut(s) 663
PspXI VCTCGAGB 1 cut(s) 316
PsuI RGATCY 2 cut(s) 559, 803
RsaI GTAC 4 cut(s) 390, 592, 596, 972
RsaNI GTAC 4 cut(s) 389, 591, 595, 971
SaqAI TTAA 3 cut(s) 285, 368, 740
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 5 cut(s) 145, 559, 803, 940, 1080
Sau96I GGNCC 1 cut(s) 663
ScrFI CCNGG 3 cut(s) 94, 620, 911
SfaNI GCATC 1 cut(s) 286
SfoI GGCGCC 1 cut(s) 15
Sfr274I CTCGAG 1 cut(s) 316
SlaI CTCGAG 1 cut(s) 316
SmlI CTYRAG 2 cut(s) 316, 901
SmoI CTYRAG 2 cut(s) 316, 901
SnaBI TACGTA 1 cut(s) 594
Sse9I AATT 4 cut(s) 150, 227, 654, 712
SseBI AGGCCT 1 cut(s) 175
SsiI CCGC 3 cut(s) 815, 839, 886
SspDI GGCGCC 1 cut(s) 13
SspMI CTAG 3 cut(s) 468, 474, 1162
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 3 cut(s) 92, 618, 909
TaaI ACNGT 3 cut(s) 25, 348, 1124
TaiI ACGT 3 cut(s) 59, 596, 960
TaqI TCGA 5 cut(s) 53, 148, 317, 556, 648
TasI AATT 4 cut(s) 150, 227, 654, 712
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 6 cut(s) 134, 184, 305, 492, 553, 842
Tru1I TTAA 3 cut(s) 285, 368, 740
Tru9I TTAA 3 cut(s) 285, 368, 740
TscAI CASTG 2 cut(s) 607, 943
TseFI GTSAC 1 cut(s) 1124
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 1124
TspDTI ATGAA 9 cut(s) 168, 221, 258, 300, 342, 392, 403, 666, 930
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 2 cut(s) 607, 943
XagI CCTNNNNNAGG 2 cut(s) 510, 567
XapI RAATTY 2 cut(s) 227, 654
XhoI CTCGAG 1 cut(s) 316
XmnI GAANNNNTTC 1 cut(s) 399
XspI CTAG 3 cut(s) 468, 474, 1162
ZraI GACGTC 1 cut(s) 958
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.