Rw4G011410

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003798
Physical Location & Seq
Forward (+)
26944854 .. 26945852
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011410.1

Sequence Viewer

Length: 999 bp
ATGTCAAGGCCTTCATTGAATCAACGAAAACACAAGTCCAAGCAGTCGTCTAAGAGACGAGAATTTCATGCTCATAATGAAGATAACAAAGTGTTGACAAGAAAAGTCCGGATAATGTTTAACGACCCCGATGCTACTGATTCTTCATCCTCGAGCGAAGATGAAACGATGGAGACTGCACAGTATGAGAAGAAAAAATCATTAAAAAAATCAAAAAGGTTTGTACAGGAAGTCATTCATTCTTTCTTCATGCATTCAGGTAGGGTAGTTGGTTGTGAAGCCCCACCAGAGACAAGAACGACTAGAACTAGCTCAGGAAGAAAGCGATTCTCGTCTTCACCTTTCAGAGGTGTACGGCAAAGAAAATATGGTAAATGGGCTGCTGAGATTCGAGATCCTATCAAAGGTGGCCGACTTTGGTTGGGTACGTACGCCACTGCCGAGGAAGCTTCCCAGGCTTACGAGACCATGAGGTGTAAGTTCGATGAAATTTTGAGGGCCACTACAACCAGTATTTCTGACAAAAACAACAAAGATGACAAGAGTAATTCTGACCACGAACACCTATTTCTTTTAAGAAAAGCAACTCCAATAACAGCTTCCAACTCTGGTGGCAACGGCCTTTTGTCCCTTAAGGGATCTCCGGTTGCGGTTCTTGATTTGGGAAAGAATGCGGATTCAGACAAGGAAAACATTGGCACTAATAATGTTGGACAACTGCGGATACCATTGGACTTGAGTTTCCTGGATGAAGCATTGCTGTCTCTCCCCACTGATCAGCAACAGCAAGACGTCTTCAACTTTGTACCAGATAATGAGGAGCTTGACAAACTTGCCTCCTCCATTGCCTCCTCCTCTTTAGAGGAACTTGGTAAGTATTTTGACGGAATGTATTGTAGCATTGATGATATAAAGATCGGTGGAGTTGATGATGATAACCCCACCAATCTTCCTGACTGTGAGGACTTTGACGATATTCTAATAGTTCAATCTAACTAA

Protein Analysis

332

Amino Acids

37.21

Weight (kDa)

6.04

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 115 - 162 1.1e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 795
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 3 cut(s) 650, 674, 721
AclWI GGATC 2 cut(s) 389, 646
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 62, 489
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 5 cut(s) 225, 354, 427, 431, 807
AfiI CCNNNNNNNGG 2 cut(s) 347, 404
AflII CTTAAG 1 cut(s) 632
AgsI TTSAA 3 cut(s) 19, 799, 989
AjnI CCWGG 2 cut(s) 453, 744
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 4 cut(s) 312, 449, 599, 823
AluI AGCT 4 cut(s) 312, 449, 599, 823
Alw26I GTCTC 5 cut(s) 49, 167, 284, 458, 768
AlwI GGATC 2 cut(s) 389, 646
Ama87I CYCGRG 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 4 cut(s) 8, 409, 498, 619
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 2 cut(s) 62, 489
Asp700I GAANNNNTTC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 330
AvaI CYCGRG 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 327, 787
BbvI GCAGC 1 cut(s) 367
BccI CCATC 1 cut(s) 163
BceAI ACGGC 2 cut(s) 371, 634
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 2 cut(s) 455, 746
BciVI GTATCC 1 cut(s) 717
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 5 cut(s) 49, 167, 284, 458, 768
BfaI CTAG 2 cut(s) 303, 309
BfrI CTTAAG 1 cut(s) 632
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 455, 746
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 498
BmrFI CCNGG 2 cut(s) 455, 746
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 2 cut(s) 327, 787
Bpu10I CCTNAGC 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 757
BsaAI YACGTR 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 441, 453
BsaWI WCCGGW 2 cut(s) 108, 643
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 404
Bse1I ACTGG 1 cut(s) 510
Bse3DI GCAATG 2 cut(s) 755, 843
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 2 cut(s) 455, 746
BseDI CCNNGG 2 cut(s) 441, 453
BseGI GGATG 2 cut(s) 146, 754
BseLI CCNNNNNNNGG 2 cut(s) 347, 404
BseMI GCAATG 2 cut(s) 755, 843
BseMII CTCAG 2 cut(s) 327, 375
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 4 cut(s) 829, 833, 841, 844
BseXI GCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 162
BshFI GGCC 4 cut(s) 10, 411, 500, 621
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 2 cut(s) 109, 644
BsiWI CGTACG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 347, 404
BsmAI GTCTC 5 cut(s) 49, 167, 284, 458, 768
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 2 cut(s) 253, 676
BsnI GGCC 4 cut(s) 10, 411, 500, 621
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 151
Bsp13I TCCGGA 1 cut(s) 108
Bsp1407I TGTACA 1 cut(s) 223
Bsp143I GATC 4 cut(s) 394, 638, 775, 915
BspACI CCGC 3 cut(s) 650, 674, 721
BspANI GGCC 4 cut(s) 10, 411, 500, 621
BspCNI CTCAG 2 cut(s) 326, 376
BspEI TCCGGA 1 cut(s) 108
BspPI GGATC 2 cut(s) 389, 646
BspTI CTTAAG 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 755, 843
BsrGI TGTACA 1 cut(s) 223
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 2 cut(s) 441, 453
BssMI GATC 4 cut(s) 394, 638, 775, 915
BssNI GRCGYC 1 cut(s) 792
Bst2UI CCWGG 2 cut(s) 455, 746
Bst4CI ACNGT 2 cut(s) 183, 959
BstACI GRCGYC 1 cut(s) 792
BstAFI CTTAAG 1 cut(s) 632
BstAUI TGTACA 1 cut(s) 223
BstBAI YACGTR 1 cut(s) 429
BstDEI CTNAG 3 cut(s) 51, 313, 384
BstENI CCTNNNNNAGG 2 cut(s) 345, 402
BstF5I GGATG 2 cut(s) 146, 754
BstKTI GATC 4 cut(s) 397, 641, 778, 918
BstMAI GTCTC 5 cut(s) 49, 167, 284, 458, 768
BstMBI GATC 4 cut(s) 394, 638, 775, 915
BstMWI GCNNNNNNNGC 2 cut(s) 446, 455
BstNI CCWGG 2 cut(s) 455, 746
BstSCI CCNGG 2 cut(s) 453, 744
BstSNI TACGTA 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 367
BstV2I GAAGAC 2 cut(s) 327, 787
BstX2I RGATCY 2 cut(s) 394, 638
BstYI RGATCY 2 cut(s) 394, 638
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 4 cut(s) 10, 411, 500, 621
BtsCI GGATG 2 cut(s) 146, 754
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 435, 771
Cfr13I GGNCC 1 cut(s) 498
Csp6I GTAC 5 cut(s) 224, 353, 426, 430, 806
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 3 cut(s) 68, 250, 469
CviQI GTAC 5 cut(s) 224, 353, 426, 430, 806
DdeI CTNAG 3 cut(s) 51, 313, 384
DpnI GATC 4 cut(s) 396, 640, 777, 917
DpnII GATC 4 cut(s) 394, 638, 775, 915
EaeI YGGCCR 1 cut(s) 409
Eco105I TACGTA 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 151
EcoNI CCTNNNNNAGG 2 cut(s) 345, 402
EcoRII CCWGG 2 cut(s) 453, 744
EcoT22I ATGCAT 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 3 cut(s) 71, 253, 472
FaiI YATR 7 cut(s) 69, 75, 186, 251, 369, 470, 911
FaqI GGGAC 1 cut(s) 613
FatI CATG 3 cut(s) 67, 249, 468
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 2 cut(s) 133, 761
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 2 cut(s) 303, 309
GluI GCNGC 1 cut(s) 381
HaeIII GGCC 4 cut(s) 10, 411, 500, 621
HapII CCGG 2 cut(s) 109, 644
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 3 cut(s) 71, 253, 472
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 5 cut(s) 19, 140, 327, 388, 677
HpaII CCGG 2 cut(s) 109, 644
HphI GGTGA 1 cut(s) 330
Hpy166II GTNNAC 2 cut(s) 96, 353
Hpy188I TCNGA 4 cut(s) 347, 520, 553, 682
Hpy188III TCNNGA 5 cut(s) 109, 315, 392, 656, 953
Hpy8I GTNNAC 2 cut(s) 96, 353
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 2 cut(s) 183, 959
HpyCH4IV ACGT 2 cut(s) 428, 792
HpyCH4V TGCA 2 cut(s) 179, 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 455
HpyF3I CTNAG 3 cut(s) 51, 313, 384
HpySE526I ACGT 2 cut(s) 428, 792
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 3 cut(s) 71, 253, 472
Kpn2I TCCGGA 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 4 cut(s) 394, 638, 775, 915
LmnI GCTCC 1 cut(s) 820
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 303, 309
MaeII ACGT 2 cut(s) 428, 792
MalI GATC 4 cut(s) 396, 640, 777, 917
MboI GATC 4 cut(s) 394, 638, 775, 915
MboII GAAGA 9 cut(s) 92, 135, 170, 202, 238, 327, 330, 787, 941
MflI RGATCY 2 cut(s) 394, 638
MluCI AATT 3 cut(s) 62, 489, 547
MmeI TCCRAC 2 cut(s) 627, 691
Mph1103I ATGCAT 1 cut(s) 255
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 4 cut(s) 120, 203, 575, 633
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 2 cut(s) 109, 644
MspR9I CCNGG 2 cut(s) 455, 746
Mva1269I GAATGC 2 cut(s) 253, 676
MvaI CCWGG 2 cut(s) 455, 746
MwoI GCNNNNNNNGC 2 cut(s) 446, 455
NdeII GATC 4 cut(s) 394, 638, 775, 915
NlaIII CATG 3 cut(s) 71, 253, 472
NmeAIII GCCGAG 1 cut(s) 466
NsiI ATGCAT 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 151
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PctI GAATGC 2 cut(s) 253, 676
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 5 cut(s) 19, 140, 327, 388, 677
Pfl23II CGTACG 1 cut(s) 429
PfoI TCCNGGA 1 cut(s) 744
PkrI GCNGC 1 cut(s) 382
Ppu21I YACGTR 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 453, 744
PspGI CCWGG 2 cut(s) 453, 744
PspLI CGTACG 1 cut(s) 429
PspPI GGNCC 1 cut(s) 498
PspXI VCTCGAGB 1 cut(s) 151
PsuI RGATCY 2 cut(s) 394, 638
RsaI GTAC 5 cut(s) 225, 354, 427, 431, 807
RsaNI GTAC 5 cut(s) 224, 353, 426, 430, 806
SaqAI TTAA 4 cut(s) 120, 203, 575, 633
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 4 cut(s) 394, 638, 775, 915
Sau96I GGNCC 1 cut(s) 498
ScrFI CCNGG 2 cut(s) 455, 746
SfaNI GCATC 1 cut(s) 121
Sfr274I CTCGAG 1 cut(s) 151
SlaI CTCGAG 1 cut(s) 151
SmlI CTYRAG 3 cut(s) 151, 632, 736
SmoI CTYRAG 3 cut(s) 151, 632, 736
SnaBI TACGTA 1 cut(s) 429
Sse9I AATT 3 cut(s) 62, 489, 547
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 3 cut(s) 650, 674, 721
SspMI CTAG 2 cut(s) 303, 309
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 453, 744
TaaI ACNGT 2 cut(s) 183, 959
TaiI ACGT 2 cut(s) 431, 795
TaqI TCGA 3 cut(s) 152, 391, 483
TasI AATT 3 cut(s) 62, 489, 547
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 5 cut(s) 19, 140, 327, 388, 677
Tru1I TTAA 4 cut(s) 120, 203, 575, 633
Tru9I TTAA 4 cut(s) 120, 203, 575, 633
TscAI CASTG 2 cut(s) 442, 778
TseI GCWGC 1 cut(s) 380
TspDTI ATGAA 8 cut(s) 56, 93, 135, 177, 227, 238, 501, 765
TspGWI ACGGA 1 cut(s) 900
TspRI CASTG 2 cut(s) 442, 778
Vha464I CTTAAG 1 cut(s) 632
XagI CCTNNNNNAGG 2 cut(s) 345, 402
XapI RAATTY 2 cut(s) 62, 489
XhoI CTCGAG 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 2 cut(s) 303, 309
ZraI GACGTC 1 cut(s) 793
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.