MD16G1043500.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003798
Physical Location & Seq
Reverse (-)
3058483 .. 3060008
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1043500.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGCCTGAGCCTCGGAGACAGCTGGTAAATCAGCGGAACAGTATGAGAAAGTCCAAGAGAGAACTATTTCCTGCTCAACAGTCCAAGATTATGAGGAAAGTCCGTATTATTTGTTACGATCCCGACGCCACTGATGACTCATCTTCGAGTGAAGATGAAGGAGGAAGAGAAAGAAGCTTTGCTAAAAACCCAAGACGCTTCGTTCGTGAAATTACTTTCTGTCAACCCAATGTTGCGCTCCCGCCGCCGAAGGCCGCCGCTGAGCCGGAGAGCTCAGGTCAAGACAGCAACTACGGTGGAGTTAAAACCCTGAAACCCAGCAACAAGAAGGTGATTTTCACTAAATCCCATCGGCAGTCCTCTTCTCCTTACAGAGGAGTGAGGCAGCGCAAATGGGGGAAATGGGCAGCTGAGATTCGTGACCCTTTCAAAGGGGCTCGTATCTGGCTTGGTACTTTCAACACTGCAGAGGACGCTTCCAAGGCCTATGAGGCGAAGCGGCTTGAAATTGAAGAAATGGTGGCAGCTGCTCCGGCTGCCGTTTCAGCCTCTGATAAAAGCAACACCACCTCGGCTTCAGCTGTCGTTTCCAACAACTGTTCCTACAACAACGTTGAACCTGTTTTGTCTGAGGATTCCGACTGTGTTCTGTCTCAGAGGTCTCCTTCGTCAGTGCTTGATTTGGAGACATCTGCTTCTAATAACATTCAGAATGGTGCTGATTTGGAGAAGGAGATTGCCGATGATTCGACAAATCTTGGAGAGCTACAAATACCCGATTTGGGTTTTCTTGATGATACATTCGGCCCTCTTCATTTTGATCAAGAGATAAACTTGGGACCGGAGCTCGATTCTCTTTACTTAGAGGATTTCGGGCAGTACTTCGACGATTACAGCAGCATTGAGGATATTCAGATTCCCGGATTTGAAAGCGATGAGCCGAGCAGTCTTCCCGACTGTGACTTTGAGGATCTTGGCAAGGATGACATTGCTTGCTGGTTGGATGAACCCCTCAACATAGTATGCCAATAA

Protein Analysis

344

Amino Acids

38.15

Weight (kDa)

4.61

Isoelectric Point (pI)

59.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 124 - 172 2.9e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 34, 242, 245, 255, 258, 499
AclI AACGTT 1 cut(s) 612
AclWI GGATC 2 cut(s) 113, 978
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 454, 881
AfiI CCNNNNNNNGG 3 cut(s) 374, 431, 782
AgsI TTSAA 6 cut(s) 430, 460, 506, 512, 617, 929
AluBI AGCT 8 cut(s) 22, 177, 273, 410, 527, 581, 766, 847
AluI AGCT 8 cut(s) 22, 177, 273, 410, 527, 581, 766, 847
Alw21I GWGCWC 2 cut(s) 275, 849
Alw26I GTCTC 4 cut(s) 10, 657, 666, 680
AlwI GGATC 2 cut(s) 113, 978
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 3 cut(s) 252, 483, 805
ApeKI GCWGC 6 cut(s) 385, 407, 524, 527, 536, 897
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 947, 979
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 2 cut(s) 238, 390
AspS9I GGNCC 2 cut(s) 806, 839
AsuC2I CCSGG 1 cut(s) 921
AsuHPI GGTGA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 839
BanII GRGCYC 3 cut(s) 275, 439, 849
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 2 cut(s) 275, 849
BbvI GCAGC 6 cut(s) 397, 419, 514, 523, 536, 909
BccI CCATC 1 cut(s) 357
BceAI ACGGC 1 cut(s) 524
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 921
BcoDI GTCTC 4 cut(s) 10, 657, 666, 680
BfmI CTRYAG 1 cut(s) 465
BglI GCCNNNNNGGC 1 cut(s) 491
BlpI GCTNAGC 1 cut(s) 261
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 1 cut(s) 921
Bme18I GGWCC 1 cut(s) 839
BmgT120I GGNCC 2 cut(s) 806, 839
BmiI GGNNCC 1 cut(s) 840
BmrFI CCNGG 1 cut(s) 921
BpiI GAAGAC 1 cut(s) 941
Bpu10I CCTNAGC 2 cut(s) 6, 274
Bpu1102I GCTNAGC 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 921
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 11, 480, 570
BsaWI WCCGGW 1 cut(s) 841
Bsc4I CCNNNNNNNGG 3 cut(s) 374, 431, 782
Bse3DI GCAATG 1 cut(s) 987
BseDI CCNNGG 3 cut(s) 11, 480, 570
BseGI GGATG 2 cut(s) 988, 1009
BseLI CCNNNNNNNGG 3 cut(s) 374, 431, 782
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 5 cut(s) 252, 288, 402, 621, 668
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 6 cut(s) 397, 419, 514, 523, 536, 909
BseYI CCCAGC 1 cut(s) 317
BshFI GGCC 3 cut(s) 254, 485, 807
BsiHKAI GWGCWC 2 cut(s) 275, 849
BsiSI CCGG 4 cut(s) 266, 533, 842, 921
BslFI GGGAC 1 cut(s) 852
BslI CCNNNNNNNGG 3 cut(s) 374, 431, 782
BsmAI GTCTC 4 cut(s) 10, 657, 666, 680
BsmFI GGGAC 1 cut(s) 852
BsnI GGCC 3 cut(s) 254, 485, 807
Bso31I GGTCTC 1 cut(s) 666
Bsp1286I GDGCHC 3 cut(s) 275, 439, 849
Bsp143I GATC 3 cut(s) 118, 820, 970
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 6 cut(s) 34, 242, 245, 255, 258, 499
BspANI GGCC 3 cut(s) 254, 485, 807
BspCNI CTCAG 5 cut(s) 253, 287, 403, 622, 667
BspLI GGNNCC 1 cut(s) 840
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 2 cut(s) 113, 978
BspTNI GGTCTC 1 cut(s) 666
BsrDI GCAATG 1 cut(s) 987
BssECI CCNNGG 3 cut(s) 11, 480, 570
BssMI GATC 3 cut(s) 118, 820, 970
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 480
Bst4CI ACNGT 6 cut(s) 41, 81, 296, 599, 644, 959
Bst6I CTCTTC 3 cut(s) 160, 367, 816
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 994
BstDEI CTNAG 7 cut(s) 6, 261, 274, 411, 630, 654, 862
BstENI CCTNNNNNAGG 2 cut(s) 372, 429
BstF5I GGATG 2 cut(s) 988, 1009
BstHHI GCGC 2 cut(s) 238, 390
BstKTI GATC 3 cut(s) 121, 823, 973
BstMAI GTCTC 4 cut(s) 10, 657, 666, 680
BstMBI GATC 3 cut(s) 118, 820, 970
BstMWI GCNNNNNNNGC 7 cut(s) 244, 473, 482, 491, 533, 536, 545
BstSCI CCNGG 1 cut(s) 919
BstSFI CTRYAG 1 cut(s) 465
BstV1I GCAGC 6 cut(s) 397, 419, 514, 523, 536, 909
BstV2I GAAGAC 1 cut(s) 941
BstX2I RGATCY 1 cut(s) 970
BstYI RGATCY 1 cut(s) 970
BsuRI GGCC 3 cut(s) 254, 485, 807
BtgZI GCGATG 1 cut(s) 948
BtsCI GGATG 2 cut(s) 988, 1009
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 129, 462, 678
Cac8I GCNNGC 1 cut(s) 994
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 2 cut(s) 238, 390
Cfr13I GGNCC 2 cut(s) 806, 839
CseI GACGC 3 cut(s) 134, 204, 482
Csp6I GTAC 2 cut(s) 453, 880
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviQI GTAC 2 cut(s) 453, 880
DdeI CTNAG 7 cut(s) 6, 261, 274, 411, 630, 654, 862
DpnI GATC 3 cut(s) 120, 822, 972
DpnII GATC 3 cut(s) 118, 820, 970
Eam1104I CTCTTC 3 cut(s) 160, 367, 816
EarI CTCTTC 3 cut(s) 160, 367, 816
Ecl136II GAGCTC 2 cut(s) 273, 847
Eco130I CCWWGG 1 cut(s) 480
Eco147I AGGCCT 1 cut(s) 485
Eco24I GRGCYC 3 cut(s) 275, 439, 849
Eco31I GGTCTC 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 839
Eco53kI GAGCTC 2 cut(s) 273, 847
Eco57I CTGAAG 1 cut(s) 561
EcoICRI GAGCTC 2 cut(s) 273, 847
EcoNI CCTNNNNNAGG 2 cut(s) 372, 429
EcoT14I CCWWGG 1 cut(s) 480
EcoT38I GRGCYC 3 cut(s) 275, 439, 849
ErhI CCWWGG 1 cut(s) 480
FaiI YATR 5 cut(s) 44, 92, 489, 1019, 1024
FaqI GGGAC 1 cut(s) 852
FauI CCCGC 1 cut(s) 249
FbaI TGATCA 1 cut(s) 820
FokI GGATG 2 cut(s) 995, 1016
FriOI GRGCYC 3 cut(s) 275, 439, 849
GlaI GCGC 2 cut(s) 237, 389
GsaI CCCAGC 1 cut(s) 321
HaeIII GGCC 3 cut(s) 254, 485, 807
HapII CCGG 4 cut(s) 266, 533, 842, 921
HgaI GACGC 3 cut(s) 134, 204, 482
HhaI GCGC 2 cut(s) 238, 390
Hin1I GRCGYC 1 cut(s) 126
Hin6I GCGC 2 cut(s) 236, 388
HinP1I GCGC 2 cut(s) 236, 388
HincII GTYRAC 1 cut(s) 224
HindII GTYRAC 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 6 cut(s) 137, 415, 635, 746, 851, 916
HpaII CCGG 4 cut(s) 266, 533, 842, 921
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 7 cut(s) 15, 553, 631, 640, 657, 711, 915
Hpy188III TCNNGA 7 cut(s) 122, 206, 281, 419, 791, 824, 953
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 2 cut(s) 128, 890
HpyAV CCTTC 5 cut(s) 152, 244, 322, 675, 724
HpyCH4III ACNGT 6 cut(s) 41, 81, 296, 599, 644, 959
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 7 cut(s) 244, 473, 482, 491, 533, 536, 545
HpyF3I CTNAG 7 cut(s) 6, 261, 274, 411, 630, 654, 862
HpySE526I ACGT 1 cut(s) 612
Hsp92I GRCGYC 1 cut(s) 126
HspAI GCGC 2 cut(s) 236, 388
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 3 cut(s) 118, 820, 970
LmnI GCTCC 3 cut(s) 243, 535, 844
Lsp1109I GCAGC 6 cut(s) 397, 419, 514, 523, 536, 909
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 3 cut(s) 113, 419, 959
MalI GATC 3 cut(s) 120, 822, 972
MboI GATC 3 cut(s) 118, 820, 970
MboII GAAGA 7 cut(s) 135, 164, 177, 354, 524, 803, 941
MflI RGATCY 1 cut(s) 970
MhlI GDGCHC 3 cut(s) 275, 439, 849
MluCI AATT 2 cut(s) 210, 507
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 3 cut(s) 615, 663, 981
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 303
MspA1I CMGCKG 6 cut(s) 22, 34, 260, 410, 527, 581
MspI CCGG 4 cut(s) 266, 533, 842, 921
MspR9I CCNGG 1 cut(s) 921
MwoI GCNNNNNNNGC 7 cut(s) 244, 473, 482, 491, 533, 536, 545
NciI CCSGG 1 cut(s) 921
NdeII GATC 3 cut(s) 118, 820, 970
NlaIV GGNNCC 1 cut(s) 840
NmeAIII GCCGAG 2 cut(s) 551, 966
NmuCI GTSAC 2 cut(s) 419, 959
PceI AGGCCT 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 5 cut(s) 415, 635, 746, 851, 916
PfoI TCCNGGA 1 cut(s) 919
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 275, 849
Psp1406I AACGTT 1 cut(s) 612
PspFI CCCAGC 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 840
PspPI GGNCC 2 cut(s) 806, 839
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 970
PvuII CAGCTG 4 cut(s) 22, 410, 527, 581
RsaI GTAC 2 cut(s) 454, 881
RsaNI GTAC 2 cut(s) 453, 880
SacI GAGCTC 2 cut(s) 275, 849
SaqAI TTAA 1 cut(s) 303
Sau3AI GATC 3 cut(s) 118, 820, 970
Sau96I GGNCC 2 cut(s) 806, 839
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 921
SduI GDGCHC 3 cut(s) 275, 439, 849
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 839
Sse9I AATT 2 cut(s) 210, 507
SseBI AGGCCT 1 cut(s) 485
SsiI CCGC 6 cut(s) 34, 242, 245, 255, 258, 499
SstI GAGCTC 2 cut(s) 275, 849
StuI AGGCCT 1 cut(s) 485
StyD4I CCNGG 1 cut(s) 919
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 6 cut(s) 41, 81, 296, 599, 644, 959
TaiI ACGT 1 cut(s) 615
TaqI TCGA 4 cut(s) 146, 749, 849, 885
TasI AATT 2 cut(s) 210, 507
TatI WGTACW 1 cut(s) 879
TauI GCSGC 4 cut(s) 247, 257, 260, 502
TfiI GAWTC 5 cut(s) 415, 635, 746, 851, 916
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 3 cut(s) 136, 469, 678
TseFI GTSAC 2 cut(s) 419, 959
TseI GCWGC 6 cut(s) 385, 407, 524, 527, 536, 897
Tsp45I GTSAC 2 cut(s) 419, 959
TspDTI ATGAA 3 cut(s) 171, 803, 1020
TspGWI ACGGA 1 cut(s) 92
TspRI CASTG 3 cut(s) 136, 469, 678
VpaK11BI GGWCC 1 cut(s) 839
XagI CCTNNNNNAGG 2 cut(s) 372, 429
XmnI GAANNNNTTC 1 cut(s) 66
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.