pycom16g03760

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003798
Physical Location & Seq
Reverse (-)
2326090 .. 2327115
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03760.1

Sequence Viewer

Length: 1026 bp
ATGCCTGAGCCTCAGAGACAGCTGGTAAATCAGCGGAACAGTATGAGAAAGTCCAAGAGAGAACTATTTCCTGCTCAACAGTCCAAGATTACGAGGAAAGTTCGTATTATTTGTTACGATCCCGACGCCACTGATGACTCATCTTCGAGCGAAGATGAAGGAGGAAGAAAGAGAAGCTTTGCTAAAAACCCAAGACGCTTCGTTCGTGAAATTACTTTCTGTCAACCCAATGTTGCGCTCCCGCCGCCGAAGGCCGCCGCTGAGCCGGAGAGCTCAGGTCAAGACAGCAACTACGGTGGAGTTAAAACCCTGAAACCCAGCAACAAGAAGGTGAAATTCACTAAATCCCATCGGCAGTCCTCTTCTCCTTACAGAGGAGTGAGACAGCGCAAATGGGGGAAATGGGCAGCTGAGATTCGCGACCCTTTCAAAGGGGCTCGTATCTGGCTTGGGACTTTCAACACTGCAGAGGACGCTTCCAAGGCCTATGAGGCGAAGCGGCTTGAAATTGAAGAAATGGTGGCTGCTGCTCCGACCGCCGTTTCAGCCTCTGATAAAAGCAACAACACCTCGGCCTCAGCTGTCGTTTCCAACAACTGTTCCTACAACAACGCTGAACCGTCTGAGGATTCCGACTGTGTCCTGTCTCAGAGGTCTCCTTCGTCAGTGCTTGATTTGGAGACATCTGCTTCTAATAACATTCAGAATGGTGCTGATTTGGAGAAGGAGATTGCCGATGATTCGACAAATCTTGGAGAGCTGCAAATACCCGATTTGGGTTTTCTTGATGATTCATTGGGCCCTCTTCATTTTGATCAAGACATAACCTTGGGACCGGAGCTCGATTCTCTTTACTTAGAGGATTTCGGGCAGTACTTCGACGATTACAGCAGCATTGAGGATATTCAGATTCCCGGATTTGAAAGCGATGAGCCGAGCAGTCTTCCCGACTGTGACTTTGAGGATCTTGGCAAGGATGACATTGCTTGCTGGTTGGATGAACCCCTCAACATAGTATGCCAATAA

Protein Analysis

342

Amino Acids

37.84

Weight (kDa)

4.67

Isoelectric Point (pI)

59.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 124 - 172 2.9e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 420
AciI CCGC 7 cut(s) 34, 242, 245, 255, 258, 499, 537
AclWI GGATC 2 cut(s) 113, 972
AcsI RAATTY 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 875
AfiI CCNNNNNNNGG 3 cut(s) 374, 431, 776
AgsI TTSAA 5 cut(s) 430, 460, 506, 512, 923
AluBI AGCT 7 cut(s) 22, 177, 273, 410, 581, 760, 841
AluI AGCT 7 cut(s) 22, 177, 273, 410, 581, 760, 841
Alw21I GWGCWC 2 cut(s) 275, 843
Alw26I GTCTC 5 cut(s) 10, 376, 651, 660, 674
AlwI GGATC 2 cut(s) 113, 972
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 4 cut(s) 252, 483, 573, 799
ApaI GGGCCC 1 cut(s) 803
ApeKI GCWGC 5 cut(s) 407, 524, 527, 760, 891
ApoI RAATTY 1 cut(s) 335
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 941, 973
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 2 cut(s) 238, 390
AspS9I GGNCC 3 cut(s) 799, 800, 833
AsuC2I CCSGG 1 cut(s) 915
AsuHPI GGTGA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 803
BanII GRGCYC 4 cut(s) 275, 439, 803, 843
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 2 cut(s) 275, 843
BbvCI CCTCAGC 1 cut(s) 577
BbvI GCAGC 5 cut(s) 419, 511, 514, 747, 903
BccI CCATC 1 cut(s) 357
BceAI ACGGC 1 cut(s) 524
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 1 cut(s) 915
BcoDI GTCTC 5 cut(s) 10, 376, 651, 660, 674
BfmI CTRYAG 1 cut(s) 465
BglI GCCNNNNNGGC 1 cut(s) 491
BisI GCNGC 9 cut(s) 245, 255, 258, 408, 500, 525, 528, 761, 892
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 9 cut(s) 246, 256, 259, 409, 501, 526, 529, 762, 893
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 1 cut(s) 915
Bme18I GGWCC 1 cut(s) 833
BmgT120I GGNCC 3 cut(s) 799, 800, 833
BmiI GGNNCC 2 cut(s) 801, 834
BmrFI CCNGG 1 cut(s) 915
BpiI GAAGAC 1 cut(s) 935
Bpu10I CCTNAGC 3 cut(s) 6, 274, 577
Bpu1102I GCTNAGC 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 3 cut(s) 480, 570, 828
BsaWI WCCGGW 1 cut(s) 835
Bsc4I CCNNNNNNNGG 3 cut(s) 374, 431, 776
Bse3DI GCAATG 1 cut(s) 981
BseDI CCNNGG 3 cut(s) 480, 570, 828
BseGI GGATG 2 cut(s) 982, 1003
BseLI CCNNNNNNNGG 3 cut(s) 374, 431, 776
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 7 cut(s) 26, 252, 288, 402, 591, 615, 662
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 5 cut(s) 419, 511, 514, 747, 903
BseYI CCCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 4 cut(s) 254, 485, 575, 801
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 275, 843
BsiSI CCGG 3 cut(s) 266, 836, 915
BslFI GGGAC 2 cut(s) 466, 846
BslI CCNNNNNNNGG 3 cut(s) 374, 431, 776
BsmAI GTCTC 5 cut(s) 10, 376, 651, 660, 674
BsmFI GGGAC 2 cut(s) 466, 846
BsnI GGCC 4 cut(s) 254, 485, 575, 801
Bso31I GGTCTC 1 cut(s) 660
Bsp120I GGGCCC 1 cut(s) 799
Bsp1286I GDGCHC 4 cut(s) 275, 439, 803, 843
Bsp143I GATC 3 cut(s) 118, 814, 964
Bsp1720I GCTNAGC 1 cut(s) 261
Bsp68I TCGCGA 1 cut(s) 420
BspACI CCGC 7 cut(s) 34, 242, 245, 255, 258, 499, 537
BspANI GGCC 4 cut(s) 254, 485, 575, 801
BspCNI CTCAG 7 cut(s) 25, 253, 287, 403, 590, 616, 661
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 2 cut(s) 801, 834
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 2 cut(s) 113, 972
BspTNI GGTCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 981
BssECI CCNNGG 3 cut(s) 480, 570, 828
BssMI GATC 3 cut(s) 118, 814, 964
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 480, 828
Bst4CI ACNGT 7 cut(s) 41, 81, 296, 599, 621, 638, 953
Bst6I CTCTTC 2 cut(s) 367, 810
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 988
BstDEI CTNAG 9 cut(s) 6, 12, 261, 274, 411, 577, 624, 648, 856
BstENI CCTNNNNNAGG 2 cut(s) 372, 429
BstF5I GGATG 2 cut(s) 982, 1003
BstFNI CGCG 1 cut(s) 420
BstHHI GCGC 2 cut(s) 238, 390
BstKTI GATC 3 cut(s) 121, 817, 967
BstMAI GTCTC 5 cut(s) 10, 376, 651, 660, 674
BstMBI GATC 3 cut(s) 118, 814, 964
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 6 cut(s) 244, 473, 482, 491, 536, 545
BstSCI CCNGG 1 cut(s) 913
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 803
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 5 cut(s) 419, 511, 514, 747, 903
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 1 cut(s) 964
BstYI RGATCY 1 cut(s) 964
BsuRI GGCC 4 cut(s) 254, 485, 575, 801
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 982, 1003
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 3 cut(s) 129, 462, 672
BtuMI TCGCGA 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 988
CaiI CAGNNNCTG 1 cut(s) 551
CfoI GCGC 2 cut(s) 238, 390
Cfr13I GGNCC 3 cut(s) 799, 800, 833
CseI GACGC 3 cut(s) 134, 204, 482
Csp6I GTAC 1 cut(s) 874
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 9 cut(s) 6, 12, 261, 274, 411, 577, 624, 648, 856
DpnI GATC 3 cut(s) 120, 816, 966
DpnII GATC 3 cut(s) 118, 814, 964
Eam1104I CTCTTC 2 cut(s) 367, 810
EarI CTCTTC 2 cut(s) 367, 810
Ecl136II GAGCTC 2 cut(s) 273, 841
Eco130I CCWWGG 2 cut(s) 480, 828
Eco147I AGGCCT 1 cut(s) 485
Eco24I GRGCYC 4 cut(s) 275, 439, 803, 843
Eco31I GGTCTC 1 cut(s) 660
Eco47I GGWCC 1 cut(s) 833
Eco53kI GAGCTC 2 cut(s) 273, 841
EcoICRI GAGCTC 2 cut(s) 273, 841
EcoNI CCTNNNNNAGG 2 cut(s) 372, 429
EcoO109I RGGNCCY 1 cut(s) 800
EcoT14I CCWWGG 2 cut(s) 480, 828
EcoT38I GRGCYC 4 cut(s) 275, 439, 803, 843
ErhI CCWWGG 2 cut(s) 480, 828
FaiI YATR 5 cut(s) 44, 489, 824, 1013, 1018
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 2 cut(s) 466, 846
FauI CCCGC 1 cut(s) 249
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 9 cut(s) 245, 255, 258, 408, 500, 525, 528, 761, 892
FokI GGATG 2 cut(s) 989, 1010
FriOI GRGCYC 4 cut(s) 275, 439, 803, 843
Fsp4HI GCNGC 9 cut(s) 245, 255, 258, 408, 500, 525, 528, 761, 892
GlaI GCGC 2 cut(s) 237, 389
GluI GCNGC 9 cut(s) 245, 255, 258, 408, 500, 525, 528, 761, 892
GsaI CCCAGC 1 cut(s) 321
HaeIII GGCC 4 cut(s) 254, 485, 575, 801
HapII CCGG 3 cut(s) 266, 836, 915
HgaI GACGC 3 cut(s) 134, 204, 482
HhaI GCGC 2 cut(s) 238, 390
Hin1I GRCGYC 1 cut(s) 126
Hin6I GCGC 2 cut(s) 236, 388
HinP1I GCGC 2 cut(s) 236, 388
HincII GTYRAC 1 cut(s) 224
HindII GTYRAC 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 7 cut(s) 137, 415, 629, 740, 791, 845, 910
HpaII CCGG 3 cut(s) 266, 836, 915
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 8 cut(s) 15, 534, 553, 625, 634, 651, 705, 909
Hpy188III TCNNGA 7 cut(s) 122, 206, 281, 419, 785, 818, 947
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 2 cut(s) 128, 884
HpyAV CCTTC 5 cut(s) 152, 244, 322, 669, 718
HpyCH4III ACNGT 7 cut(s) 41, 81, 296, 599, 621, 638, 953
HpyCH4V TGCA 2 cut(s) 467, 763
HpyF10VI GCNNNNNNNGC 6 cut(s) 244, 473, 482, 491, 536, 545
HpyF3I CTNAG 9 cut(s) 6, 12, 261, 274, 411, 577, 624, 648, 856
Hsp92I GRCGYC 1 cut(s) 126
HspAI GCGC 2 cut(s) 236, 388
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 3 cut(s) 118, 814, 964
LmnI GCTCC 3 cut(s) 243, 535, 838
Lsp1109I GCAGC 5 cut(s) 419, 511, 514, 747, 903
MaeIII GTNAC 2 cut(s) 113, 953
MalI GATC 3 cut(s) 120, 816, 966
MboI GATC 3 cut(s) 118, 814, 964
MboII GAAGA 7 cut(s) 135, 164, 177, 354, 524, 797, 935
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 4 cut(s) 275, 439, 803, 843
MluCI AATT 3 cut(s) 210, 335, 507
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 4 cut(s) 557, 615, 657, 975
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 1 cut(s) 303
MspA1I CMGCKG 5 cut(s) 22, 34, 260, 410, 581
MspI CCGG 3 cut(s) 266, 836, 915
MspR9I CCNGG 1 cut(s) 915
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 6 cut(s) 244, 473, 482, 491, 536, 545
NciI CCSGG 1 cut(s) 915
NdeII GATC 3 cut(s) 118, 814, 964
NlaIV GGNNCC 2 cut(s) 801, 834
NmeAIII GCCGAG 2 cut(s) 551, 960
NmuCI GTSAC 1 cut(s) 953
NruI TCGCGA 1 cut(s) 420
PceI AGGCCT 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 6 cut(s) 415, 629, 740, 791, 845, 910
PflFI GACNNNGTC 1 cut(s) 638
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 9 cut(s) 246, 256, 259, 409, 501, 526, 529, 762, 893
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 275, 843
PspFI CCCAGC 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 801, 834
PspOMI GGGCCC 1 cut(s) 799
PspPI GGNCC 3 cut(s) 799, 800, 833
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 551
PsuI RGATCY 1 cut(s) 964
PsyI GACNNNGTC 1 cut(s) 638
PvuII CAGCTG 3 cut(s) 22, 410, 581
RruI TCGCGA 1 cut(s) 420
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
SacI GAGCTC 2 cut(s) 275, 843
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 9 cut(s) 245, 255, 258, 408, 500, 525, 528, 761, 892
Sau3AI GATC 3 cut(s) 118, 814, 964
Sau96I GGNCC 3 cut(s) 799, 800, 833
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 1 cut(s) 915
SduI GDGCHC 4 cut(s) 275, 439, 803, 843
SfcI CTRYAG 1 cut(s) 465
SinI GGWCC 1 cut(s) 833
Sse9I AATT 3 cut(s) 210, 335, 507
SseBI AGGCCT 1 cut(s) 485
SsiI CCGC 7 cut(s) 34, 242, 245, 255, 258, 499, 537
SstI GAGCTC 2 cut(s) 275, 843
StuI AGGCCT 1 cut(s) 485
StyD4I CCNGG 1 cut(s) 913
StyI CCWWGG 2 cut(s) 480, 828
TaaI ACNGT 7 cut(s) 41, 81, 296, 599, 621, 638, 953
TaqI TCGA 4 cut(s) 146, 743, 843, 879
TasI AATT 3 cut(s) 210, 335, 507
TatI WGTACW 1 cut(s) 873
TauI GCSGC 4 cut(s) 247, 257, 260, 502
TfiI GAWTC 6 cut(s) 415, 629, 740, 791, 845, 910
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 3 cut(s) 136, 469, 672
TseFI GTSAC 1 cut(s) 953
TseI GCWGC 5 cut(s) 407, 524, 527, 760, 891
Tsp45I GTSAC 1 cut(s) 953
TspDTI ATGAA 4 cut(s) 171, 783, 797, 1014
TspRI CASTG 3 cut(s) 136, 469, 672
Tth111I GACNNNGTC 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 833
XagI CCTNNNNNAGG 2 cut(s) 372, 429
XapI RAATTY 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 66
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.