MD13G1042600.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003798
Physical Location & Seq
Reverse (-)
2960973 .. 2963265
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1042600.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGCTGTGCCTCGGAAACAGCGAAACAGAATGAGAAAGTCCAAGAGAGAACCATTTCCTGATCACCAGTCCAAGATTATGAGGAAAGTTCGCATTATCTGTTACGATCCCGACGCCACTGATGACTCATCTTCAAGCGAGGACGAAGGAGGAAGAGAGAGAAGATTTCCCAACAACCCAAAACGCTTCGTTCGTGAAATTACTTTCTGCCCAACCGCGCACCCGAAGCCGGGCACAGCTGAGCAAGAGAGCTCAAGTCAAGACAGCAATGGAGTCAGAACCCCAAAACCCAGCAAGAAGGCGACTTTCACCAAGTCCCACCGGCAGTCCTCTTCTCCTTACAGAGGCGTGAGGCAGCGCAAATGGGGGAAATGGGCTGCTGAGATTCGTGATCCTTTCAAAGGGGTTCGTCTCTGGCTTGGTACTTTCAACACTGCGGAGGACGCCTCAAAGGCTTATGAGGCCAAGCGGCTCGAATTTGAAGAAATGTTGGCAGCTGCTACCTCAGCTTCAGCTGTCGTTTCCAACCAGAATTCCTACAACAACGTTGAACCTGTTTCGTCTGAGGATTCTGACTCTGTCGTCTCTCAGAGCTCTCCTTCTTGTGTGCTTGATTTGGAGACATCTGCTTCCAATAACATTCGGAATGGTGCTGATTTGGAGAAGGAGATTGCCGATGAGTCGACAAATCTTGAAGAGCTGCAAATACCCGATTTGGGTTTACTTGATGAGACATTGGGCCCTCTTCATTTTGATCCAGAGCTAAACTTGGGACCGGAGCTCGATTCGCTTTACCTGGAGGATTTTGGGCAGTTGTTCGACAATTACAGCAGCATTGAGGATATACAGATTCCCGGGTTCGAAAGCGATGAGCCGAGCAGTCTACCCGACTGTGACTTTGAGGATCTTGGCAAGGATGACATTGCTTGCTGGTTGGATGAACCCCTCAACATAGTATGCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

322

Amino Acids

36.02

Weight (kDa)

4.73

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 114 - 162 6.8e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
AccI GTMKAC 2 cut(s) 683, 883
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 216, 437, 469
AclI AACGTT 1 cut(s) 546
AclWI GGATC 4 cut(s) 101, 386, 749, 912
AcsI RAATTY 2 cut(s) 476, 532
AcuI CTGAAG 1 cut(s) 495
AcyI GRCGYC 2 cut(s) 114, 444
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 5 cut(s) 229, 230, 344, 401, 716
AgsI TTSAA 6 cut(s) 135, 400, 430, 482, 551, 695
AjnI CCWGG 1 cut(s) 795
AluBI AGCT 9 cut(s) 239, 252, 497, 509, 515, 594, 700, 763, 781
AluI AGCT 9 cut(s) 239, 252, 497, 509, 515, 594, 700, 763, 781
Alw21I GWGCWC 3 cut(s) 254, 596, 783
Alw26I GTCTC 4 cut(s) 416, 589, 614, 725
AlwI GGATC 4 cut(s) 101, 386, 749, 912
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 2 cut(s) 462, 739
ApaI GGGCCC 1 cut(s) 743
ApeKI GCWGC 6 cut(s) 355, 377, 494, 497, 700, 831
ApoI RAATTY 2 cut(s) 476, 532
ArsI GACNNNNNNTTYG 2 cut(s) 881, 913
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 2 cut(s) 220, 360
AspS9I GGNCC 3 cut(s) 739, 740, 773
AsuC2I CCSGG 3 cut(s) 231, 855, 856
AsuHPI GGTGA 2 cut(s) 56, 301
AsuII TTCGAA 1 cut(s) 861
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 1 cut(s) 773
BaeGI GKGCMC 2 cut(s) 236, 743
BanII GRGCYC 4 cut(s) 254, 596, 743, 783
Bbv12I GWGCWC 3 cut(s) 254, 596, 783
BbvCI CCTCAGC 1 cut(s) 505
BbvI GCAGC 6 cut(s) 364, 367, 484, 506, 687, 843
BciT130I CCWGG 1 cut(s) 797
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 3 cut(s) 231, 855, 856
BcoDI GTCTC 4 cut(s) 416, 589, 614, 725
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 7 cut(s) 356, 378, 470, 495, 498, 701, 832
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 7 cut(s) 357, 379, 471, 496, 499, 702, 833
Bme1390I CCNGG 4 cut(s) 231, 797, 855, 856
Bme18I GGWCC 1 cut(s) 773
BmeT110I CYCGRG 1 cut(s) 854
BmgT120I GGNCC 3 cut(s) 739, 740, 773
BmiI GGNNCC 2 cut(s) 741, 774
BmrFI CCNGG 4 cut(s) 231, 797, 855, 856
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 505
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 238
BpuMI CCSGG 3 cut(s) 231, 855, 856
BsaHI GRCGYC 2 cut(s) 114, 444
BsaJI CCNNGG 2 cut(s) 11, 854
BsaWI WCCGGW 1 cut(s) 775
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 230, 344, 401, 716
Bse118I RCCGGY 1 cut(s) 321
Bse1I ACTGG 1 cut(s) 67
Bse3DI GCAATG 2 cut(s) 274, 921
BseBI CCWGG 1 cut(s) 797
BseDI CCNNGG 2 cut(s) 11, 854
BseGI GGATG 2 cut(s) 922, 943
BseLI CCNNNNNNNGG 5 cut(s) 229, 230, 344, 401, 716
BseMI GCAATG 2 cut(s) 274, 921
BseMII CTCAG 5 cut(s) 231, 372, 519, 555, 602
BseNI ACTGG 1 cut(s) 67
BseSI GKGCMC 2 cut(s) 236, 743
BseXI GCAGC 6 cut(s) 364, 367, 484, 506, 687, 843
BseYI CCCAGC 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 2 cut(s) 464, 741
BsiHKAI GWGCWC 3 cut(s) 254, 596, 783
BsiHKCI CYCGRG 1 cut(s) 854
BsiSI CCGG 4 cut(s) 230, 322, 776, 855
BslFI GGGAC 2 cut(s) 301, 786
BslI CCNNNNNNNGG 5 cut(s) 229, 230, 344, 401, 716
BsmAI GTCTC 4 cut(s) 416, 589, 614, 725
BsmBI CGTCTC 2 cut(s) 416, 589
BsmFI GGGAC 2 cut(s) 301, 786
BsnI GGCC 2 cut(s) 464, 741
BsoBI CYCGRG 1 cut(s) 854
Bsp119I TTCGAA 1 cut(s) 861
Bsp120I GGGCCC 1 cut(s) 739
Bsp1286I GDGCHC 5 cut(s) 236, 254, 596, 743, 783
Bsp143I GATC 5 cut(s) 61, 106, 391, 754, 904
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 3 cut(s) 216, 437, 469
BspANI GGCC 2 cut(s) 464, 741
BspCNI CTCAG 5 cut(s) 232, 373, 518, 556, 601
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 2 cut(s) 741, 774
BspPI GGATC 4 cut(s) 101, 386, 749, 912
BspQI GCTCTTC 1 cut(s) 690
BspT104I TTCGAA 1 cut(s) 861
BsrDI GCAATG 2 cut(s) 274, 921
BsrFI RCCGGY 1 cut(s) 321
BsrI ACTGG 1 cut(s) 67
BssAI RCCGGY 1 cut(s) 321
BssECI CCNNGG 2 cut(s) 11, 854
BssMI GATC 5 cut(s) 61, 106, 391, 754, 904
BssNI GRCGYC 2 cut(s) 114, 444
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 1 cut(s) 893
Bst6I CTCTTC 4 cut(s) 148, 337, 690, 750
BstACI GRCGYC 2 cut(s) 114, 444
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 1 cut(s) 928
BstDEI CTNAG 5 cut(s) 240, 381, 505, 564, 588
BstENI CCTNNNNNAGG 2 cut(s) 342, 399
BstF5I GGATG 2 cut(s) 922, 943
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 2 cut(s) 220, 360
BstKTI GATC 5 cut(s) 64, 109, 394, 757, 907
BstMAI GTCTC 4 cut(s) 416, 589, 614, 725
BstMBI GATC 5 cut(s) 61, 106, 391, 754, 904
BstMWI GCNNNNNNNGC 6 cut(s) 226, 443, 452, 461, 506, 787
BstNI CCWGG 1 cut(s) 797
BstSCI CCNGG 4 cut(s) 229, 795, 853, 854
BstSLI GKGCMC 2 cut(s) 236, 743
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 6 cut(s) 364, 367, 484, 506, 687, 843
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
BsuRI GGCC 2 cut(s) 464, 741
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 2 cut(s) 922, 943
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 117, 432
Cac8I GCNNGC 1 cut(s) 928
CfoI GCGC 2 cut(s) 220, 360
Cfr10I RCCGGY 1 cut(s) 321
Cfr13I GGNCC 3 cut(s) 739, 740, 773
Cfr9I CCCGGG 1 cut(s) 854
CseI GACGC 2 cut(s) 122, 452
Csp6I GTAC 1 cut(s) 423
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 5 cut(s) 240, 381, 505, 564, 588
DpnI GATC 5 cut(s) 63, 108, 393, 756, 906
DpnII GATC 5 cut(s) 61, 106, 391, 754, 904
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
Eam1104I CTCTTC 4 cut(s) 148, 337, 690, 750
EarI CTCTTC 4 cut(s) 148, 337, 690, 750
Ecl136II GAGCTC 3 cut(s) 252, 594, 781
Eco24I GRGCYC 4 cut(s) 254, 596, 743, 783
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 3 cut(s) 252, 594, 781
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 854
EcoICRI GAGCTC 3 cut(s) 252, 594, 781
EcoNI CCTNNNNNAGG 2 cut(s) 342, 399
EcoO109I RGGNCCY 1 cut(s) 740
EcoRI GAATTC 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 795
EcoT38I GRGCYC 4 cut(s) 254, 596, 743, 783
Esp3I CGTCTC 2 cut(s) 416, 589
FaiI YATR 5 cut(s) 80, 459, 845, 953, 958
FaqI GGGAC 2 cut(s) 301, 786
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 2 cut(s) 683, 883
Fnu4HI GCNGC 7 cut(s) 356, 378, 470, 495, 498, 701, 832
FokI GGATG 2 cut(s) 929, 950
FriOI GRGCYC 4 cut(s) 254, 596, 743, 783
Fsp4HI GCNGC 7 cut(s) 356, 378, 470, 495, 498, 701, 832
GlaI GCGC 2 cut(s) 219, 359
GluI GCNGC 7 cut(s) 356, 378, 470, 495, 498, 701, 832
GsaI CCCAGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 2 cut(s) 464, 741
HapII CCGG 4 cut(s) 230, 322, 776, 855
HgaI GACGC 2 cut(s) 122, 452
HhaI GCGC 2 cut(s) 220, 360
Hin1I GRCGYC 2 cut(s) 114, 444
Hin6I GCGC 2 cut(s) 218, 358
HinP1I GCGC 2 cut(s) 218, 358
HincII GTYRAC 1 cut(s) 684
HindII GTYRAC 1 cut(s) 684
HinfI GANTC 8 cut(s) 125, 273, 385, 569, 575, 680, 785, 850
HpaII CCGG 4 cut(s) 230, 322, 776, 855
HphI GGTGA 2 cut(s) 56, 301
Hpy166II GTNNAC 3 cut(s) 684, 722, 884
Hpy188I TCNGA 6 cut(s) 15, 278, 565, 574, 591, 645
Hpy188III TCNNGA 7 cut(s) 59, 110, 194, 260, 389, 692, 758
Hpy8I GTNNAC 3 cut(s) 684, 722, 884
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 4 cut(s) 140, 292, 609, 658
HpyCH4III ACNGT 1 cut(s) 893
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 1 cut(s) 703
HpyF10VI GCNNNNNNNGC 6 cut(s) 226, 443, 452, 461, 506, 787
HpyF3I CTNAG 5 cut(s) 240, 381, 505, 564, 588
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 2 cut(s) 114, 444
HspAI GCGC 2 cut(s) 218, 358
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 5 cut(s) 61, 106, 391, 754, 904
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 1 cut(s) 778
Lsp1109I GCAGC 6 cut(s) 364, 367, 484, 506, 687, 843
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 2 cut(s) 101, 893
MalI GATC 5 cut(s) 63, 108, 393, 756, 906
MboI GATC 5 cut(s) 61, 106, 391, 754, 904
MboII GAAGA 7 cut(s) 123, 165, 174, 324, 494, 707, 737
MflI RGATCY 1 cut(s) 904
MhlI GDGCHC 5 cut(s) 236, 254, 596, 743, 783
MluCI AATT 4 cut(s) 198, 476, 532, 823
MlyI GAGTC 4 cut(s) 119, 282, 569, 689
MmeI TCCRAC 2 cut(s) 549, 915
MroXI GAANNNNTTC 1 cut(s) 54
MspA1I CMGCKG 3 cut(s) 239, 497, 515
MspI CCGG 4 cut(s) 230, 322, 776, 855
MspR9I CCNGG 4 cut(s) 231, 797, 855, 856
MvaI CCWGG 1 cut(s) 797
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 6 cut(s) 226, 443, 452, 461, 506, 787
NciI CCSGG 3 cut(s) 231, 855, 856
NdeII GATC 5 cut(s) 61, 106, 391, 754, 904
NlaIV GGNNCC 2 cut(s) 741, 774
NmeAIII GCCGAG 1 cut(s) 900
NmuCI GTSAC 1 cut(s) 893
NspV TTCGAA 1 cut(s) 861
PciSI GCTCTTC 1 cut(s) 690
PcsI WCGNNNNNNNCGW 2 cut(s) 19, 190
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 4 cut(s) 385, 569, 785, 850
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 7 cut(s) 357, 379, 471, 496, 499, 702, 833
PleI GAGTC 4 cut(s) 119, 281, 569, 688
PpsI GAGTC 4 cut(s) 119, 281, 569, 688
Psp124BI GAGCTC 3 cut(s) 254, 596, 783
Psp1406I AACGTT 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 795
PspN4I GGNNCC 2 cut(s) 741, 774
PspOMI GGGCCC 1 cut(s) 739
PspPI GGNCC 3 cut(s) 739, 740, 773
PsuI RGATCY 1 cut(s) 904
PsyI GACNNNGTC 1 cut(s) 578
PvuII CAGCTG 3 cut(s) 239, 497, 515
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SacI GAGCTC 3 cut(s) 254, 596, 783
SalI GTCGAC 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 690
SatI GCNGC 7 cut(s) 356, 378, 470, 495, 498, 701, 832
Sau3AI GATC 5 cut(s) 61, 106, 391, 754, 904
Sau96I GGNCC 3 cut(s) 739, 740, 773
SchI GAGTC 4 cut(s) 119, 282, 569, 689
ScrFI CCNGG 4 cut(s) 231, 797, 855, 856
SduI GDGCHC 5 cut(s) 236, 254, 596, 743, 783
SfuI TTCGAA 1 cut(s) 861
SinI GGWCC 1 cut(s) 773
SmaI CCCGGG 1 cut(s) 856
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
Sse9I AATT 4 cut(s) 198, 476, 532, 823
SsiI CCGC 3 cut(s) 216, 437, 469
SstI GAGCTC 3 cut(s) 254, 596, 783
StyD4I CCNGG 4 cut(s) 229, 795, 853, 854
TaaI ACNGT 1 cut(s) 893
TaiI ACGT 1 cut(s) 549
TaqI TCGA 5 cut(s) 474, 683, 783, 819, 861
TasI AATT 4 cut(s) 198, 476, 532, 823
TauI GCSGC 1 cut(s) 472
TfiI GAWTC 4 cut(s) 385, 569, 785, 850
TscAI CASTG 2 cut(s) 124, 439
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 6 cut(s) 355, 377, 494, 497, 700, 831
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 2 cut(s) 737, 954
TspMI CCCGGG 1 cut(s) 854
TspRI CASTG 2 cut(s) 124, 439
Tth111I GACNNNGTC 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 773
XagI CCTNNNNNAGG 2 cut(s) 342, 399
XapI RAATTY 2 cut(s) 476, 532
XmaI CCCGGG 1 cut(s) 854
XmiI GTMKAC 2 cut(s) 683, 883
XmnI GAANNNNTTC 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.