RLG00000006603

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003798
Physical Location & Seq
Forward (+)
8900231 .. 8901816
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006603

Sequence Viewer

Length: 1260 bp
ATGTCGGCTTGTTTTGATTTGGTGTTGATTGGGGCTCTGTTGCAGCAGCTCTGGATTTTATCTTCAAATTCTTCTTCTTGCTCTTCTTCTGGTTCAAAGGCTTGTTCTGCTATCTGCTTTATGCTCTTCTGCTGGTTCTGGCAGAGATTCAGCCTGGTATATCCGCCGTTTTCGATGAGTTTGAAACCCGTAAGGGATTTTTCGAAAATGCCCGAGCCTCGTAATCCACGGCTGAACCAACGGAATAGTTTCAAGAAGTCTAAGATGGAACAACTGTTTCCTGCTGAAGAGCCCAAGATGGTGAGGAAGGTTCGGATAATCTGCTCCGATCCTGATGCTACTGACGACTCATCTTCCGAAGATGAAGGAATTGATAGAAAACCTCACAAACGCATGGTGAAGGAAATCACTCTTCCTTTTAATTTCACGCGCCCATCCAAGCTTGTTGTTGAGCCGGAGGGTTCTTCCAACAATGGAGTCAAAACCCCAACGCCTAAACCCAAGTTGGGGATTTTGGCTAAAACCTCGAAAAAATCATCCAATTCGCCCTTTCGAGGTGTGAGGCAAAGAAAATGGGGCAAATGGGCGGCTGAAATTCGTGACCCTTTCAGCAAGACTAGGGTCTGGTTGGGAACCTACAACACTGCTGAGGATGCTTCCAAGGCCTACGAGGAAAGGAGGCTCTACTTCGAAAAGATGCTCTCTTTGGCCACAGCTTCAGAGGTCAGTTCTGAGAAAAGCAACACCACCACCACCACCACCACCTCTTGTTCTGCCGTCGTCTCTCGGTCGGTCAACAACACCCAACGACCTGCTGTTTCATCCGAGGAGGAATCTGACTGCGTCTACTCCCAAAGGTCTCCGGCTTCAGTTCTGGATGTTGAGACCTGTGCCTCCAACAACATTCGCAGTGGTGGTGATTTGGAGAATGGTGGAGATATGGAGACTGAAGATGTTGGGACTCATGCCCTCGGGGAGCTGCCAATTCCCAACGACTTGGGATTCATGGACGAGCAGCCATTTGGGCCGGAGCTCAACTTTGATGGGATTGAACCAGTAAGCTTTCAAGCGGAGATCGACTCGCTTCTTCTGGATGAAATTGGGCAGTTTTTCAACGATTTTGGTAGCATTGATGATGTCCATATTGGTGGTTTTGAAGGAAATGAGCCCAGCAATCTTCCTGATTGTGACTTTGGGGATCTTGGCAAGGAGGACTTTGCTTGCTGGATGAATGAAACCTCCAACATACAATGCCAATAA

Protein Analysis

420

Amino Acids

46.58

Weight (kDa)

4.94

Isoelectric Point (pI)

60.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 184 - 232 1.4e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 820
AccI GTMKAC 1 cut(s) 846
AccII CGCG 1 cut(s) 430
AciI CCGC 3 cut(s) 164, 587, 1070
AclWI GGATC 2 cut(s) 323, 1206
AcoI YGGCCR 1 cut(s) 708
AcsI RAATTY 2 cut(s) 67, 594
AcuI CTGAAG 4 cut(s) 306, 702, 852, 969
AfiI CCNNNNNNNGG 3 cut(s) 506, 507, 554
AgsI TTSAA 8 cut(s) 66, 96, 184, 253, 1052, 1067, 1114, 1157
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 6 cut(s) 49, 442, 716, 979, 1033, 1062
AluI AGCT 6 cut(s) 49, 442, 716, 979, 1033, 1062
Alw21I GWGCWC 1 cut(s) 1035
Alw26I GTCTC 4 cut(s) 787, 864, 878, 938
AlwI GGATC 2 cut(s) 323, 1206
Ama87I CYCGRG 2 cut(s) 212, 971
AoxI GGCC 3 cut(s) 663, 708, 1025
ApeKI GCWGC 4 cut(s) 43, 46, 979, 1015
ApoI RAATTY 2 cut(s) 67, 594
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 1025
AsuHPI GGTGA 3 cut(s) 313, 409, 929
AsuII TTCGAA 2 cut(s) 203, 690
AvaI CYCGRG 2 cut(s) 212, 971
BalI TGGCCA 1 cut(s) 710
BanII GRGCYC 4 cut(s) 37, 294, 1035, 1170
Bbv12I GWGCWC 1 cut(s) 1035
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 4 cut(s) 55, 58, 966, 1027
BccI CCATC 4 cut(s) 259, 292, 442, 1037
BceAI ACGGC 3 cut(s) 151, 245, 761
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 155
BcoDI GTCTC 4 cut(s) 787, 864, 878, 938
BfaI CTAG 1 cut(s) 618
BfuAI ACCTGC 1 cut(s) 820
BglI GCCNNNNNGGC 1 cut(s) 1024
BisI GCNGC 5 cut(s) 44, 47, 588, 980, 1016
BlsI GCNGC 5 cut(s) 45, 48, 589, 981, 1017
Bme1390I CCNGG 1 cut(s) 155
BmeT110I CYCGRG 2 cut(s) 212, 971
BmgT120I GGNCC 1 cut(s) 1025
BmiI GGNNCC 1 cut(s) 634
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 3 cut(s) 325, 643, 687
BoxI GACNNNNGTC 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 1064, 1096
Bpu10I CCTNAGC 1 cut(s) 648
Bpu14I TTCGAA 2 cut(s) 203, 690
BsaI GGTCTC 2 cut(s) 864, 878
BsaJI CCNNGG 4 cut(s) 227, 660, 825, 970
Bsc4I CCNNNNNNNGG 3 cut(s) 506, 507, 554
Bse1I ACTGG 1 cut(s) 1055
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 4 cut(s) 227, 660, 825, 970
BseGI GGATG 7 cut(s) 434, 536, 658, 821, 883, 1099, 1233
BseLI CCNNNNNNNGG 3 cut(s) 506, 507, 554
BseMII CTCAG 2 cut(s) 639, 723
BseNI ACTGG 1 cut(s) 1055
BseRI GAGGAG 1 cut(s) 842
BseXI GCAGC 4 cut(s) 55, 58, 966, 1027
BseYI CCCAGC 1 cut(s) 1169
Bsh1236I CGCG 1 cut(s) 430
Bsh1285I CGRYCG 1 cut(s) 791
BshFI GGCC 3 cut(s) 665, 710, 1027
BsiEI CGRYCG 1 cut(s) 791
BsiHKAI GWGCWC 1 cut(s) 1035
BsiHKCI CYCGRG 2 cut(s) 212, 971
BsiSI CCGG 3 cut(s) 455, 863, 1028
BslFI GGGAC 1 cut(s) 973
BslI CCNNNNNNNGG 3 cut(s) 506, 507, 554
BsmAI GTCTC 4 cut(s) 787, 864, 878, 938
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 1 cut(s) 973
BsnI GGCC 3 cut(s) 665, 710, 1027
Bso31I GGTCTC 2 cut(s) 864, 878
BsoBI CYCGRG 2 cut(s) 212, 971
Bsp119I TTCGAA 2 cut(s) 203, 690
Bsp1286I GDGCHC 4 cut(s) 37, 294, 1035, 1170
Bsp143I GATC 3 cut(s) 328, 1074, 1198
BspACI CCGC 3 cut(s) 164, 587, 1070
BspANI GGCC 3 cut(s) 665, 710, 1027
BspCNI CTCAG 2 cut(s) 640, 724
BspFNI CGCG 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 634
BspMI ACCTGC 1 cut(s) 820
BspPI GGATC 2 cut(s) 323, 1206
BspQI GCTCTTC 3 cut(s) 88, 131, 282
BspT104I TTCGAA 2 cut(s) 203, 690
BspTNI GGTCTC 2 cut(s) 864, 878
BsrI ACTGG 1 cut(s) 1055
BssECI CCNNGG 4 cut(s) 227, 660, 825, 970
BssMI GATC 3 cut(s) 328, 1074, 1198
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 4 cut(s) 88, 131, 282, 417
BstBI TTCGAA 2 cut(s) 203, 690
BstC8I GCNNGC 1 cut(s) 1222
BstDEI CTNAG 3 cut(s) 261, 648, 732
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 7 cut(s) 434, 536, 658, 821, 883, 1099, 1233
BstFNI CGCG 1 cut(s) 430
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 3 cut(s) 331, 1077, 1201
BstMAI GTCTC 4 cut(s) 787, 864, 878, 938
BstMBI GATC 3 cut(s) 328, 1074, 1198
BstMCI CGRYCG 1 cut(s) 791
BstMWI GCNNNNNNNGC 4 cut(s) 107, 653, 662, 1024
BstNI CCWGG 1 cut(s) 155
BstPAI GACNNNNGTC 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 153
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 4 cut(s) 55, 58, 966, 1027
BstX2I RGATCY 1 cut(s) 1198
BstXI CCANNNNNNTGG 2 cut(s) 997, 1148
BstYI RGATCY 1 cut(s) 1198
BsuRI GGCC 3 cut(s) 665, 710, 1027
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 7 cut(s) 434, 536, 658, 821, 883, 1099, 1233
BtsI GCAGTG 2 cut(s) 642, 916
BtsIMutI CAGTG 2 cut(s) 642, 916
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 1 cut(s) 1222
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 1025
CseI GACGC 1 cut(s) 832
CviAII CATG 3 cut(s) 394, 965, 1006
DdeI CTNAG 3 cut(s) 261, 648, 732
DpnI GATC 3 cut(s) 330, 1076, 1200
DpnII GATC 3 cut(s) 328, 1074, 1198
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 4 cut(s) 88, 131, 282, 417
EarI CTCTTC 4 cut(s) 88, 131, 282, 417
EciI GGCGGA 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 1033
Eco130I CCWWGG 1 cut(s) 660
Eco147I AGGCCT 1 cut(s) 665
Eco24I GRGCYC 4 cut(s) 37, 294, 1035, 1170
Eco31I GGTCTC 2 cut(s) 864, 878
Eco53kI GAGCTC 1 cut(s) 1033
Eco57I CTGAAG 4 cut(s) 306, 702, 852, 969
Eco88I CYCGRG 2 cut(s) 212, 971
EcoICRI GAGCTC 1 cut(s) 1033
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 4 cut(s) 37, 294, 1035, 1170
ErhI CCWWGG 1 cut(s) 660
Esp3I CGTCTC 1 cut(s) 787
FaeI CATG 3 cut(s) 397, 968, 1009
FaiI YATR 8 cut(s) 122, 160, 395, 941, 966, 1007, 1143, 1247
FalI AAGNNNNNCTT 2 cut(s) 1199, 1231
FaqI GGGAC 1 cut(s) 973
FatI CATG 3 cut(s) 393, 964, 1005
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 5 cut(s) 44, 47, 588, 980, 1016
FokI GGATG 7 cut(s) 421, 523, 665, 808, 890, 1106, 1240
FriOI GRGCYC 4 cut(s) 37, 294, 1035, 1170
Fsp4HI GCNGC 5 cut(s) 44, 47, 588, 980, 1016
FspBI CTAG 1 cut(s) 618
GlaI GCGC 1 cut(s) 431
GluI GCNGC 5 cut(s) 44, 47, 588, 980, 1016
GsaI CCCAGC 1 cut(s) 1173
HaeIII GGCC 3 cut(s) 665, 710, 1027
HapII CCGG 3 cut(s) 455, 863, 1028
HgaI GACGC 1 cut(s) 832
HhaI GCGC 1 cut(s) 432
Hin1II CATG 3 cut(s) 397, 968, 1009
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HindIII AAGCTT 2 cut(s) 440, 1060
HinfI GANTC 7 cut(s) 147, 347, 477, 833, 961, 1002, 1079
HpaII CCGG 3 cut(s) 455, 863, 1028
HphI GGTGA 3 cut(s) 313, 409, 929
Hpy166II GTNNAC 2 cut(s) 796, 847
Hpy188I TCNGA 7 cut(s) 315, 328, 358, 721, 733, 826, 838
Hpy188III TCNNGA 7 cut(s) 52, 253, 332, 599, 875, 1091, 1181
Hpy8I GTNNAC 2 cut(s) 796, 847
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 4 cut(s) 301, 359, 394, 1151
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 43
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 653, 662, 1024
HpyF3I CTNAG 3 cut(s) 261, 648, 732
Hsp92II CATG 3 cut(s) 397, 968, 1009
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 3 cut(s) 328, 1074, 1198
LguI GCTCTTC 3 cut(s) 88, 131, 282
LmnI GCTCC 3 cut(s) 329, 976, 1030
Lsp1109I GCAGC 4 cut(s) 55, 58, 966, 1027
LweI GCATC 3 cut(s) 325, 643, 687
MaeI CTAG 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 599, 1187
MalI GATC 3 cut(s) 330, 1076, 1200
MboI GATC 3 cut(s) 328, 1074, 1198
MflI RGATCY 1 cut(s) 1198
MhlI GDGCHC 4 cut(s) 37, 294, 1035, 1170
MlsI TGGCCA 1 cut(s) 710
MluCI AATT 7 cut(s) 67, 369, 421, 541, 594, 984, 1098
MluNI TGGCCA 1 cut(s) 710
MlyI GAGTC 4 cut(s) 341, 486, 955, 1073
MmeI TCCRAC 2 cut(s) 492, 921
Mox20I TGGCCA 1 cut(s) 710
MroXI GAANNNNTTC 1 cut(s) 248
MscI TGGCCA 1 cut(s) 710
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 1146
Msp20I TGGCCA 1 cut(s) 710
MspI CCGG 3 cut(s) 455, 863, 1028
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 4 cut(s) 107, 653, 662, 1024
NdeII GATC 3 cut(s) 328, 1074, 1198
NlaIII CATG 3 cut(s) 397, 968, 1009
NlaIV GGNNCC 1 cut(s) 634
NmuCI GTSAC 2 cut(s) 599, 1187
NspV TTCGAA 2 cut(s) 203, 690
PceI AGGCCT 1 cut(s) 665
PciSI GCTCTTC 3 cut(s) 88, 131, 282
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 147, 833, 1002
PflFI GACNNNGTC 1 cut(s) 842
PkrI GCNGC 5 cut(s) 45, 48, 589, 981, 1017
PleI GAGTC 4 cut(s) 341, 485, 955, 1073
PpsI GAGTC 4 cut(s) 341, 485, 955, 1073
PshAI GACNNNNGTC 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 1035
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 1 cut(s) 1025
PsuI RGATCY 1 cut(s) 1198
PsyI GACNNNGTC 1 cut(s) 842
RseI CAYNNNNRTG 1 cut(s) 1146
SacI GAGCTC 1 cut(s) 1035
SapI GCTCTTC 3 cut(s) 88, 131, 282
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 5 cut(s) 44, 47, 588, 980, 1016
Sau3AI GATC 3 cut(s) 328, 1074, 1198
Sau96I GGNCC 1 cut(s) 1025
SchI GAGTC 4 cut(s) 341, 486, 955, 1073
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 4 cut(s) 37, 294, 1035, 1170
SfaNI GCATC 3 cut(s) 325, 643, 687
SfuI TTCGAA 2 cut(s) 203, 690
SmiMI CAYNNNNRTG 1 cut(s) 1146
Sse9I AATT 7 cut(s) 67, 369, 421, 541, 594, 984, 1098
SseBI AGGCCT 1 cut(s) 665
SsiI CCGC 3 cut(s) 164, 587, 1070
SspMI CTAG 1 cut(s) 618
SstI GAGCTC 1 cut(s) 1035
StuI AGGCCT 1 cut(s) 665
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 6 cut(s) 173, 203, 527, 553, 690, 1077
TaqII GACCGA 2 cut(s) 777, 781
TasI AATT 7 cut(s) 67, 369, 421, 541, 594, 984, 1098
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 3 cut(s) 147, 833, 1002
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 2 cut(s) 649, 916
TseFI GTSAC 2 cut(s) 599, 1187
TseI GCWGC 4 cut(s) 43, 46, 979, 1015
Tsp45I GTSAC 2 cut(s) 599, 1187
TspDTI ATGAA 6 cut(s) 378, 810, 994, 1110, 1244, 1248
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 2 cut(s) 649, 916
Tth111I GACNNNGTC 1 cut(s) 842
XapI RAATTY 2 cut(s) 67, 594
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.