Rw4G029610

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003798
Physical Location & Seq
Reverse (-)
56299893 .. 56300933
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029610.1

Sequence Viewer

Length: 1041 bp
ATGCCGGAGCCTCGTAATCCACGGCTGAATCAACGGAGTAGTTTCAAGAAGTCTAAGAGGGAACAACTCTTTCCTGCTGCAGAGCCCAAGATGGTGAGAAAGGTTCGTATAATCTGCTCCGATCCTGATGCTACTGATGACTCATCTTCCGAAGATGAAGGAGTTGATAGAAAACTTCACAAACGCATGGTGAAGGAAATCACTCTTCCTTTTAATTTCACACGCCCATCTAAGCTTGTTGTTGAGCCCGAGGGTTCTTCCAACAATGGAGTCGAAACCCCAACGCCTAAACCCAAGTTGGGGGTTTTGGCTAAAACCTCGAAAAAATCATCCAATTCGCCCTTTCGAGGTGTGAGGCAAAGAAAATGGGGCAAATGGGCGGCTGAAATTCGTGACCCTTTCAGCAAGACTAGGGTCTGGTTGGGAACCTACAACACTGCTGAGGATGCTTCCAAGGCCTACGAGGAAAGGAGGCTCTACTTCGAAAAGATGCTCTCTTTGGCCACAGCTTCAGAGGTCAGCTCTGAGAAAAGCAGCACCACCACCTCTTGTTCTGCCATCGTCTCTCGGTCGGTCAACAACACCCAACAACCTGCTGTTTCATCCGAGGAGGAATCTGACTGCGTCTACTCCCAAAGGTCTCCGGCCTCAGTTCTGGATGTTGAGACCTGTGCTTCCAACAACATTCGCAGTGGTGGTGATTTGGAGAATGGTGGTGATATGGAGACTGAAGATGTTGGGACTAATGCCCTCGGGGAGCTGCCAATTCCCAATGACTTGGGATTCATGGACGAGCAGCCATTTGGGCCGGAGCTCAACTTTGATGGGATTGAACCAGTAAGCTTTCAAGCTGAGATTGACTCGCTTCTTCTGGATGAAATTGGGCAGTTTTTCAACGATTTTGGTAGCATTGATGATGTCCATATTGGTGGCTTTGAAGGAAATGAGCCCAGCAATCTTCCTGATTGTGACTTTGGGGATCTTGGCAAGGAGGACTTTGCTTGCTGGATGACTGAAACCTCCAACATACAATGCCAATAA

Protein Analysis

346

Amino Acids

38.29

Weight (kDa)

4.72

Isoelectric Point (pI)

58.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 115 - 163 1.1e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 601
AccI GTMKAC 1 cut(s) 627
AciI CCGC 1 cut(s) 380
AclWI GGATC 2 cut(s) 116, 987
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 2 cut(s) 495, 750
AfiI CCNNNNNNNGG 3 cut(s) 299, 300, 347
AgsI TTSAA 5 cut(s) 46, 833, 848, 895, 938
AluBI AGCT 7 cut(s) 235, 509, 522, 760, 814, 843, 851
AluI AGCT 7 cut(s) 235, 509, 522, 760, 814, 843, 851
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 4 cut(s) 568, 645, 659, 719
AlwI GGATC 2 cut(s) 116, 987
Ama87I CYCGRG 2 cut(s) 248, 752
AoxI GGCC 4 cut(s) 456, 501, 645, 806
ApeKI GCWGC 4 cut(s) 77, 534, 760, 796
ApoI RAATTY 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 4 cut(s) 106, 202, 710, 728
AsuII TTCGAA 1 cut(s) 483
AvaI CYCGRG 2 cut(s) 248, 752
BalI TGGCCA 1 cut(s) 503
BanII GRGCYC 4 cut(s) 87, 249, 816, 951
Bbv12I GWGCWC 1 cut(s) 816
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 4 cut(s) 64, 546, 747, 808
BccI CCATC 4 cut(s) 85, 235, 566, 818
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BcoDI GTCTC 4 cut(s) 568, 645, 659, 719
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 78
BfuAI ACCTGC 1 cut(s) 601
BglI GCCNNNNNGGC 1 cut(s) 805
BisI GCNGC 5 cut(s) 78, 381, 535, 761, 797
BlsI GCNGC 5 cut(s) 79, 382, 536, 762, 798
BmeT110I CYCGRG 2 cut(s) 248, 752
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 2 cut(s) 9, 427
BmsI GCATC 3 cut(s) 118, 436, 480
BoxI GACNNNNGTC 1 cut(s) 413
BplI GAGNNNNNCTC 4 cut(s) 506, 538, 845, 877
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 483
BsaI GGTCTC 2 cut(s) 645, 659
BsaJI CCNNGG 5 cut(s) 20, 249, 453, 606, 751
Bsc4I CCNNNNNNNGG 3 cut(s) 299, 300, 347
Bse1I ACTGG 1 cut(s) 836
BseDI CCNNGG 5 cut(s) 20, 249, 453, 606, 751
BseGI GGATG 6 cut(s) 329, 451, 602, 664, 880, 1014
BseLI CCNNNNNNNGG 3 cut(s) 299, 300, 347
BseMII CTCAG 4 cut(s) 432, 516, 663, 843
BseNI ACTGG 1 cut(s) 836
BseRI GAGGAG 1 cut(s) 623
BseXI GCAGC 4 cut(s) 64, 546, 747, 808
BseYI CCCAGC 1 cut(s) 950
Bsh1285I CGRYCG 1 cut(s) 572
BshFI GGCC 4 cut(s) 458, 503, 647, 808
BsiEI CGRYCG 1 cut(s) 572
BsiHKAI GWGCWC 1 cut(s) 816
BsiHKCI CYCGRG 2 cut(s) 248, 752
BsiSI CCGG 3 cut(s) 5, 644, 809
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 3 cut(s) 299, 300, 347
BsmAI GTCTC 4 cut(s) 568, 645, 659, 719
BsmBI CGTCTC 1 cut(s) 568
BsmFI GGGAC 1 cut(s) 754
BsnI GGCC 4 cut(s) 458, 503, 647, 808
Bso31I GGTCTC 2 cut(s) 645, 659
BsoBI CYCGRG 2 cut(s) 248, 752
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 4 cut(s) 87, 249, 816, 951
Bsp143I GATC 2 cut(s) 121, 979
BspACI CCGC 1 cut(s) 380
BspANI GGCC 4 cut(s) 458, 503, 647, 808
BspCNI CTCAG 4 cut(s) 433, 517, 662, 844
BspLI GGNNCC 2 cut(s) 9, 427
BspMAI CTGCAG 1 cut(s) 82
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 2 cut(s) 116, 987
BspT104I TTCGAA 1 cut(s) 483
BspTNI GGTCTC 2 cut(s) 645, 659
BsrI ACTGG 1 cut(s) 836
BssECI CCNNGG 5 cut(s) 20, 249, 453, 606, 751
BssMI GATC 2 cut(s) 121, 979
BssT1I CCWWGG 1 cut(s) 453
Bst6I CTCTTC 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 1 cut(s) 1003
BstDEI CTNAG 6 cut(s) 54, 231, 441, 525, 649, 852
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 6 cut(s) 329, 451, 602, 664, 880, 1014
BstKTI GATC 2 cut(s) 124, 982
BstMAI GTCTC 4 cut(s) 568, 645, 659, 719
BstMBI GATC 2 cut(s) 121, 979
BstMCI CGRYCG 1 cut(s) 572
BstMWI GCNNNNNNNGC 3 cut(s) 446, 455, 805
BstPAI GACNNNNGTC 1 cut(s) 413
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 4 cut(s) 64, 546, 747, 808
BstX2I RGATCY 1 cut(s) 979
BstXI CCANNNNNNTGG 2 cut(s) 778, 929
BstYI RGATCY 1 cut(s) 979
BsuRI GGCC 4 cut(s) 458, 503, 647, 808
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 6 cut(s) 329, 451, 602, 664, 880, 1014
BtsI GCAGTG 2 cut(s) 435, 697
BtsIMutI CAGTG 2 cut(s) 435, 697
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 1 cut(s) 1003
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 1 cut(s) 613
CviAII CATG 2 cut(s) 187, 787
DdeI CTNAG 6 cut(s) 54, 231, 441, 525, 649, 852
DpnI GATC 2 cut(s) 123, 981
DpnII GATC 2 cut(s) 121, 979
EaeI YGGCCR 1 cut(s) 501
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 4 cut(s) 87, 249, 816, 951
Eco31I GGTCTC 2 cut(s) 645, 659
Eco53kI GAGCTC 1 cut(s) 814
Eco57I CTGAAG 2 cut(s) 495, 750
Eco88I CYCGRG 2 cut(s) 248, 752
EcoICRI GAGCTC 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 4 cut(s) 87, 249, 816, 951
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 568
FaeI CATG 2 cut(s) 190, 790
FaiI YATR 6 cut(s) 110, 188, 722, 788, 924, 1028
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FaqI GGGAC 1 cut(s) 754
FatI CATG 2 cut(s) 186, 786
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 5 cut(s) 78, 381, 535, 761, 797
FokI GGATG 6 cut(s) 316, 458, 589, 671, 887, 1021
FriOI GRGCYC 4 cut(s) 87, 249, 816, 951
Fsp4HI GCNGC 5 cut(s) 78, 381, 535, 761, 797
FspBI CTAG 1 cut(s) 411
GluI GCNGC 5 cut(s) 78, 381, 535, 761, 797
GsaI CCCAGC 1 cut(s) 954
HaeIII GGCC 4 cut(s) 458, 503, 647, 808
HapII CCGG 3 cut(s) 5, 644, 809
HgaI GACGC 1 cut(s) 613
Hin1II CATG 2 cut(s) 190, 790
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 2 cut(s) 233, 841
HinfI GANTC 6 cut(s) 28, 140, 270, 614, 783, 860
HpaII CCGG 3 cut(s) 5, 644, 809
HphI GGTGA 4 cut(s) 106, 202, 710, 728
Hpy166II GTNNAC 2 cut(s) 577, 628
Hpy188I TCNGA 6 cut(s) 121, 151, 514, 526, 607, 619
Hpy188III TCNNGA 6 cut(s) 46, 125, 392, 656, 872, 962
Hpy8I GTNNAC 2 cut(s) 577, 628
HpyAV CCTTC 3 cut(s) 152, 187, 932
HpyCH4V TGCA 1 cut(s) 80
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 455, 805
HpyF3I CTNAG 6 cut(s) 54, 231, 441, 525, 649, 852
Hsp92II CATG 2 cut(s) 190, 790
Kzo9I GATC 2 cut(s) 121, 979
LmnI GCTCC 4 cut(s) 7, 122, 757, 811
Lsp1109I GCAGC 4 cut(s) 64, 546, 747, 808
LweI GCATC 3 cut(s) 118, 436, 480
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 392, 968
MalI GATC 2 cut(s) 123, 981
MboI GATC 2 cut(s) 121, 979
MboII GAAGA 7 cut(s) 138, 164, 197, 249, 743, 860, 950
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 4 cut(s) 87, 249, 816, 951
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 5 cut(s) 214, 334, 387, 765, 879
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 3 cut(s) 134, 279, 854
MmeI TCCRAC 2 cut(s) 285, 702
Mox20I TGGCCA 1 cut(s) 503
MscI TGGCCA 1 cut(s) 503
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 927
Msp20I TGGCCA 1 cut(s) 503
MspI CCGG 3 cut(s) 5, 644, 809
MwoI GCNNNNNNNGC 3 cut(s) 446, 455, 805
NdeII GATC 2 cut(s) 121, 979
NlaIII CATG 2 cut(s) 190, 790
NlaIV GGNNCC 2 cut(s) 9, 427
NmuCI GTSAC 2 cut(s) 392, 968
NspV TTCGAA 1 cut(s) 483
PceI AGGCCT 1 cut(s) 458
PfeI GAWTC 3 cut(s) 28, 614, 783
PflFI GACNNNGTC 1 cut(s) 623
PkrI GCNGC 5 cut(s) 79, 382, 536, 762, 798
PleI GAGTC 3 cut(s) 134, 278, 854
PpsI GAGTC 3 cut(s) 134, 278, 854
PshAI GACNNNNGTC 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 816
PspFI CCCAGC 1 cut(s) 950
PspN4I GGNNCC 2 cut(s) 9, 427
PspPI GGNCC 1 cut(s) 806
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 979
PsyI GACNNNGTC 1 cut(s) 623
RseI CAYNNNNRTG 1 cut(s) 927
SacI GAGCTC 1 cut(s) 816
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 5 cut(s) 78, 381, 535, 761, 797
Sau3AI GATC 2 cut(s) 121, 979
Sau96I GGNCC 1 cut(s) 806
SchI GAGTC 3 cut(s) 134, 279, 854
SduI GDGCHC 4 cut(s) 87, 249, 816, 951
SfaNI GCATC 3 cut(s) 118, 436, 480
SfcI CTRYAG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 927
Sse9I AATT 5 cut(s) 214, 334, 387, 765, 879
SseBI AGGCCT 1 cut(s) 458
SsiI CCGC 1 cut(s) 380
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 816
StuI AGGCCT 1 cut(s) 458
StyI CCWWGG 1 cut(s) 453
TaqI TCGA 4 cut(s) 273, 320, 346, 483
TaqII GACCGA 2 cut(s) 558, 562
TasI AATT 5 cut(s) 214, 334, 387, 765, 879
TauI GCSGC 1 cut(s) 383
TfiI GAWTC 3 cut(s) 28, 614, 783
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 2 cut(s) 442, 697
TseFI GTSAC 2 cut(s) 392, 968
TseI GCWGC 4 cut(s) 77, 534, 760, 796
Tsp45I GTSAC 2 cut(s) 392, 968
TspDTI ATGAA 4 cut(s) 171, 591, 775, 891
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 2 cut(s) 442, 697
Tth111I GACNNNGTC 1 cut(s) 623
XapI RAATTY 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.