RLG00000008761

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003798
Physical Location & Seq
Reverse (-)
36651822 .. 36652841
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008761

Sequence Viewer

Length: 1020 bp
ATGTCAAGGCCTTCATTGAATCAACGAAAACACAAGTCCAAGCAGTCGTCTAAGAGACGAGAATTTCATGCTCATAATGAAGATAACAAAGTGTTGACAAGAAAAGTCCGGATAATGTTTAACGACCCCGATGCTACTGATTCTTCATCCTCGAGCGAAGATGAAACGATGGAGACTGCACAGTATGAGAAGAAAAAATCATTAAAAAAATCAAAAAGGTTTGTACAGGAAGTCATTCATTCTTTCTTCATGCATTCAGGTAGGGCAGTTGGTTGTGAAGCACCACCAGAGACTAGAACGACTAGAAGTAGCTCAGGAAGAAAGCGATTCTCGTCTTCACCTTTCAGAGGTGTAAGGCAAAGAAAATATGGTAAATGGGCTGCTGAGATTCGAGATCCTATCAAAGGTGGCCGACTTTGGTTGGGTACGTACACCACTGCCGAGGAAGCTTCCCAGGCTTACGAGACCATGAGGTGTAAGTTCGATGAAATTTTGAGGGCCACGTGCACTACAACCAGTATTTCTGACAAAAGCAACAAAGATGACAAGAGTAATTCTGACCACGAACACCTATTTCTTTTAAGAAAAGCAACTCCAATAACAGCTTCCAACGACTCCGGTGGCAACGGCCTTTTGTCCCATAAGGGATCTCCGGTTGGGGTTCTTGATTTGGGAAAGAATGCAGATTCCGACAACGAAAACATTGGCACTAATAATGTTGGACAACTGCGGATCCCATTGGACTTGAGTTTCCTGGATGAAGCATTGCAGTCTCTCCCCACTGATCAGCAACGGCAAGACTTCTTCAACTTTGTACCAGATAATGAGGAGCTTGACAAACTTGCCTCCTCCATTGCCTCCTCCTCTTTAGAGGAACTTGGTAAGTATTTTGAAGGAATGTATTGTAGCATTGATGATATAAAGATCGGTGGAGTTGATGATGATAACCCCACCAATCTTCCTGACTGTGACGACTTTGACGATATTCTAATAGTTCAATCTAACTATGCCTATAACTAG

Protein Analysis

340

Amino Acids

38.09

Weight (kDa)

5.97

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 115 - 162 5.6e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 1 cut(s) 730
AclWI GGATC 4 cut(s) 389, 655, 727, 740
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 62, 489
AcvI CACGTG 1 cut(s) 504
AfaI GTAC 4 cut(s) 225, 427, 431, 816
AfiI CCNNNNNNNGG 2 cut(s) 347, 404
AgsI TTSAA 4 cut(s) 19, 808, 893, 998
AjnI CCWGG 2 cut(s) 453, 753
AjuI GAANNNNNNNTTGG 2 cut(s) 589, 621
AluBI AGCT 4 cut(s) 312, 449, 605, 832
AluI AGCT 4 cut(s) 312, 449, 605, 832
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 5 cut(s) 49, 167, 284, 458, 777
Alw44I GTGCAC 1 cut(s) 505
AlwI GGATC 4 cut(s) 389, 655, 727, 740
Ama87I CYCGRG 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 4 cut(s) 8, 409, 498, 628
ApaLI GTGCAC 1 cut(s) 505
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 2 cut(s) 62, 489
Asp700I GAANNNNTTC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 330
AvaI CYCGRG 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 509
BamHI GGATCC 1 cut(s) 732
BbrPI CACGTG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 1 cut(s) 367
BccI CCATC 1 cut(s) 163
BceAI ACGGC 2 cut(s) 643, 809
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 2 cut(s) 455, 755
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 5 cut(s) 49, 167, 284, 458, 777
BfaI CTAG 3 cut(s) 294, 303, 1018
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 455, 755
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 1 cut(s) 734
BmrFI CCNGG 2 cut(s) 455, 755
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 766
BsaAI YACGTR 2 cut(s) 429, 504
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 441, 453
BsaWI WCCGGW 3 cut(s) 108, 617, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 404
Bse1I ACTGG 1 cut(s) 516
Bse3DI GCAATG 2 cut(s) 764, 852
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 2 cut(s) 455, 755
BseDI CCNNGG 2 cut(s) 441, 453
BseGI GGATG 2 cut(s) 146, 763
BseLI CCNNNNNNNGG 2 cut(s) 347, 404
BseMI GCAATG 2 cut(s) 764, 852
BseMII CTCAG 2 cut(s) 327, 375
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 4 cut(s) 838, 842, 850, 853
BseSI GKGCMC 1 cut(s) 509
BseXI GCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 162
BshFI GGCC 4 cut(s) 10, 411, 500, 630
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 3 cut(s) 109, 618, 653
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 2 cut(s) 347, 404
BsmAI GTCTC 5 cut(s) 49, 167, 284, 458, 777
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 622
BsmI GAATGC 2 cut(s) 253, 685
BsnI GGCC 4 cut(s) 10, 411, 500, 630
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 151
Bsp1286I GDGCHC 1 cut(s) 509
Bsp13I TCCGGA 1 cut(s) 108
Bsp1407I TGTACA 1 cut(s) 223
Bsp143I GATC 5 cut(s) 394, 647, 732, 784, 924
BspACI CCGC 1 cut(s) 730
BspANI GGCC 4 cut(s) 10, 411, 500, 630
BspCNI CTCAG 2 cut(s) 326, 376
BspEI TCCGGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 734
BspPI GGATC 4 cut(s) 389, 655, 727, 740
BspTNI GGTCTC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 764, 852
BsrGI TGTACA 1 cut(s) 223
BsrI ACTGG 1 cut(s) 516
BssECI CCNNGG 2 cut(s) 441, 453
BssMI GATC 5 cut(s) 394, 647, 732, 784, 924
Bst2UI CCWGG 2 cut(s) 455, 755
Bst4CI ACNGT 2 cut(s) 183, 968
BstAUI TGTACA 1 cut(s) 223
BstBAI YACGTR 2 cut(s) 429, 504
BstDEI CTNAG 3 cut(s) 51, 313, 384
BstENI CCTNNNNNAGG 2 cut(s) 345, 402
BstF5I GGATG 2 cut(s) 146, 763
BstKTI GATC 5 cut(s) 397, 650, 735, 787, 927
BstMAI GTCTC 5 cut(s) 49, 167, 284, 458, 777
BstMBI GATC 5 cut(s) 394, 647, 732, 784, 924
BstMWI GCNNNNNNNGC 2 cut(s) 446, 455
BstNI CCWGG 2 cut(s) 455, 755
BstSCI CCNGG 2 cut(s) 453, 753
BstSLI GKGCMC 1 cut(s) 509
BstSNI TACGTA 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 367
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 3 cut(s) 394, 647, 732
BstYI RGATCY 3 cut(s) 394, 647, 732
BsuRI GGCC 4 cut(s) 10, 411, 500, 630
BtsCI GGATG 2 cut(s) 146, 763
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 435, 780
Cfr13I GGNCC 1 cut(s) 498
Csp6I GTAC 4 cut(s) 224, 426, 430, 815
CviAII CATG 3 cut(s) 68, 250, 469
CviQI GTAC 4 cut(s) 224, 426, 430, 815
DdeI CTNAG 3 cut(s) 51, 313, 384
DpnI GATC 5 cut(s) 396, 649, 734, 786, 926
DpnII GATC 5 cut(s) 394, 647, 732, 784, 924
EaeI YGGCCR 1 cut(s) 409
Eco105I TACGTA 1 cut(s) 429
Eco147I AGGCCT 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 458
Eco72I CACGTG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 151
EcoNI CCTNNNNNAGG 2 cut(s) 345, 402
EcoRII CCWGG 2 cut(s) 453, 753
EcoT22I ATGCAT 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 3 cut(s) 71, 253, 472
FaqI GGGAC 1 cut(s) 622
FatI CATG 3 cut(s) 67, 249, 468
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 2 cut(s) 133, 770
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 3 cut(s) 294, 303, 1018
GluI GCNGC 1 cut(s) 381
HaeIII GGCC 4 cut(s) 10, 411, 500, 630
HapII CCGG 3 cut(s) 109, 618, 653
Hin1II CATG 3 cut(s) 71, 253, 472
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 6 cut(s) 19, 140, 327, 388, 614, 686
HpaII CCGG 3 cut(s) 109, 618, 653
HphI GGTGA 1 cut(s) 330
Hpy166II GTNNAC 3 cut(s) 96, 432, 507
Hpy188I TCNGA 4 cut(s) 347, 526, 559, 691
Hpy188III TCNNGA 5 cut(s) 109, 315, 392, 665, 962
Hpy8I GTNNAC 3 cut(s) 96, 432, 507
HpyAV CCTTC 2 cut(s) 21, 887
HpyCH4III ACNGT 2 cut(s) 183, 968
HpyCH4IV ACGT 2 cut(s) 428, 503
HpyCH4V TGCA 5 cut(s) 179, 253, 507, 683, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 455
HpyF3I CTNAG 3 cut(s) 51, 313, 384
HpySE526I ACGT 2 cut(s) 428, 503
Hsp92II CATG 3 cut(s) 71, 253, 472
Kpn2I TCCGGA 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 5 cut(s) 394, 647, 732, 784, 924
LmnI GCTCC 1 cut(s) 829
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 1 cut(s) 121
MaeI CTAG 3 cut(s) 294, 303, 1018
MaeII ACGT 2 cut(s) 428, 503
MaeIII GTNAC 1 cut(s) 968
MalI GATC 5 cut(s) 396, 649, 734, 786, 926
MboI GATC 5 cut(s) 394, 647, 732, 784, 924
MboII GAAGA 9 cut(s) 92, 135, 170, 202, 238, 327, 330, 796, 950
MflI RGATCY 3 cut(s) 394, 647, 732
MhlI GDGCHC 1 cut(s) 509
MluCI AATT 3 cut(s) 62, 489, 553
MlyI GAGTC 1 cut(s) 608
MmeI TCCRAC 3 cut(s) 633, 700, 714
Mph1103I ATGCAT 1 cut(s) 255
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 3 cut(s) 120, 203, 581
MspI CCGG 3 cut(s) 109, 618, 653
MspR9I CCNGG 2 cut(s) 455, 755
Mva1269I GAATGC 2 cut(s) 253, 685
MvaI CCWGG 2 cut(s) 455, 755
MwoI GCNNNNNNNGC 2 cut(s) 446, 455
NdeII GATC 5 cut(s) 394, 647, 732, 784, 924
NlaIII CATG 3 cut(s) 71, 253, 472
NlaIV GGNNCC 1 cut(s) 734
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 968
NsiI ATGCAT 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 151
PceI AGGCCT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 978
PctI GAATGC 2 cut(s) 253, 685
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 5 cut(s) 19, 140, 327, 388, 686
PfoI TCCNGGA 1 cut(s) 753
PkrI GCNGC 1 cut(s) 382
PleI GAGTC 1 cut(s) 608
PmaCI CACGTG 1 cut(s) 504
PmlI CACGTG 1 cut(s) 504
PpsI GAGTC 1 cut(s) 608
Ppu21I YACGTR 2 cut(s) 429, 504
Psp6I CCWGG 2 cut(s) 453, 753
PspCI CACGTG 1 cut(s) 504
PspGI CCWGG 2 cut(s) 453, 753
PspN4I GGNNCC 1 cut(s) 734
PspPI GGNCC 1 cut(s) 498
PspXI VCTCGAGB 1 cut(s) 151
PsuI RGATCY 3 cut(s) 394, 647, 732
RsaI GTAC 4 cut(s) 225, 427, 431, 816
RsaNI GTAC 4 cut(s) 224, 426, 430, 815
SaqAI TTAA 3 cut(s) 120, 203, 581
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 5 cut(s) 394, 647, 732, 784, 924
Sau96I GGNCC 1 cut(s) 498
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 2 cut(s) 455, 755
SduI GDGCHC 1 cut(s) 509
SfaNI GCATC 1 cut(s) 121
Sfr274I CTCGAG 1 cut(s) 151
SlaI CTCGAG 1 cut(s) 151
SmlI CTYRAG 2 cut(s) 151, 745
SmoI CTYRAG 2 cut(s) 151, 745
SnaBI TACGTA 1 cut(s) 429
Sse9I AATT 3 cut(s) 62, 489, 553
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 1 cut(s) 730
SspMI CTAG 3 cut(s) 294, 303, 1018
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 453, 753
TaaI ACNGT 2 cut(s) 183, 968
TaiI ACGT 2 cut(s) 431, 506
TaqI TCGA 3 cut(s) 152, 391, 483
TasI AATT 3 cut(s) 62, 489, 553
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 5 cut(s) 19, 140, 327, 388, 686
Tru1I TTAA 3 cut(s) 120, 203, 581
Tru9I TTAA 3 cut(s) 120, 203, 581
TscAI CASTG 2 cut(s) 442, 787
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 8 cut(s) 56, 93, 135, 177, 227, 238, 501, 774
TspRI CASTG 2 cut(s) 442, 787
VneI GTGCAC 1 cut(s) 505
XagI CCTNNNNNAGG 2 cut(s) 345, 402
XapI RAATTY 2 cut(s) 62, 489
XhoI CTCGAG 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 3 cut(s) 294, 303, 1018
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.