pycom13g03730

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003798
Physical Location & Seq
Reverse (-)
2474712 .. 2475590
879 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03730.1

Sequence Viewer

Length: 879 bp
ATGGCTGTGCCTCGGAAACAGCCGCTGAATCAGCGAGACAGAATGAAAAAGTCCAATCCTGCTCACCAGTCCAAGATTACGAGGAAAGTTCGCATTATCTGTTATGATCCCGACGCCACTGATGACTCATCTTCAAGCGAGGACGAAGGAGGAAGAGAGAGAAGATTTGCCAAAACCCCAAAACGCTTCGTTCGCGAAGTTACTTTCTGCCCATCTGCGCACCCGAAGCCAGGCACCGCCGAGCCAGAGAGCTCAAGTCAAGACAGCAATGGAGTCAGAACCCCAAAACCCAGCAAGAAGGCGCTTTTCGCCAAGTCCCATCGGCAGTCCTCTTCTCCTTACAGAGGCGTGAGGCAGCGCAAATGGGGGAAATGGGCTGCTGAGATTCGTGATCCTTTCAAAGGGGTTCGTCTCTGGCTTGGTACTTTCAATACCGCGGAGGACGCCTCAAAGGCCTATGAGGCCAAGCGGCTCGAATTTGAAGAAATGTTGGCAGCTGCTACCTCAGCTTCAGCTGTCGTTTCCAACAAGAATTCCTACAACAACGTCGAACCTGTTTCATCTGAGGATTCGGACTCTGTCGTTTCTCAGAGGTCTCCTTCTTGTGTGCTTGATTTGGAGAAGGAGACTGCCGATGAGTCGACAAATCTTGAAGAGCTGCAAATACCCGATTTGGGTTTTCTTGATGAGACATTGGGCCCTCTTCTTTTTGATCAAGAGCTAAACTTGGGACCGGAGCTCGATTCGCTTTACCTGGAGGATTTTGGGCAGTTCTTCGACAATTACAGCAGCATTGAGGATATACAGATTCCCGGGTTCGAAAGCGATGAGCCGAGCAGTCTACCCGACTGTGACTTTGAGCGATCTTGGCAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

293

Amino Acids

32.78

Weight (kDa)

5.0

Isoelectric Point (pI)

57.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 114 - 162 5.9e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 219
AccB1I GGYRCC 1 cut(s) 233
AccI GTMKAC 2 cut(s) 641, 841
AccII CGCG 2 cut(s) 195, 437
AciI CCGC 5 cut(s) 23, 237, 435, 437, 469
AclWI GGATC 2 cut(s) 101, 386
AcsI RAATTY 2 cut(s) 476, 532
AcuI CTGAAG 1 cut(s) 495
AcyI GRCGYC 2 cut(s) 114, 444
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 4 cut(s) 230, 344, 401, 674
AgsI TTSAA 5 cut(s) 135, 400, 430, 482, 653
AjnI CCWGG 2 cut(s) 229, 753
AluBI AGCT 7 cut(s) 252, 497, 509, 515, 658, 721, 739
AluI AGCT 7 cut(s) 252, 497, 509, 515, 658, 721, 739
Alw21I GWGCWC 2 cut(s) 254, 741
Alw26I GTCTC 5 cut(s) 30, 416, 600, 620, 683
AlwI GGATC 2 cut(s) 101, 386
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 3 cut(s) 453, 462, 697
ApaI GGGCCC 1 cut(s) 701
ApeKI GCWGC 6 cut(s) 355, 377, 494, 497, 658, 789
ApoI RAATTY 2 cut(s) 476, 532
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
AspLEI GCGC 3 cut(s) 220, 304, 360
AspS9I GGNCC 3 cut(s) 697, 698, 731
AsuC2I CCSGG 2 cut(s) 813, 814
AsuHPI GGTGA 1 cut(s) 56
AsuII TTCGAA 1 cut(s) 819
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 1 cut(s) 731
BaeGI GKGCMC 1 cut(s) 701
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 3 cut(s) 254, 701, 741
Bbv12I GWGCWC 2 cut(s) 254, 741
BbvCI CCTCAGC 1 cut(s) 505
BbvI GCAGC 6 cut(s) 364, 367, 484, 506, 645, 801
BccI CCATC 2 cut(s) 220, 327
BciT130I CCWGG 2 cut(s) 231, 755
BclI TGATCA 1 cut(s) 712
BcnI CCSGG 2 cut(s) 813, 814
BcoDI GTCTC 5 cut(s) 30, 416, 600, 620, 683
BfoI RGCGCY 1 cut(s) 305
BglI GCCNNNNNGGC 2 cut(s) 452, 461
BisI GCNGC 8 cut(s) 23, 356, 378, 470, 495, 498, 659, 790
BlsI GCNGC 8 cut(s) 24, 357, 379, 471, 496, 499, 660, 791
Bme1390I CCNGG 4 cut(s) 231, 755, 813, 814
Bme18I GGWCC 1 cut(s) 731
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 3 cut(s) 697, 698, 731
BmiI GGNNCC 3 cut(s) 235, 699, 732
BmrFI CCNGG 4 cut(s) 231, 755, 813, 814
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 776
Bpu10I CCTNAGC 1 cut(s) 505
Bpu14I TTCGAA 1 cut(s) 819
BpuEI CTTGAG 1 cut(s) 238
BpuMI CCSGG 2 cut(s) 813, 814
BsaHI GRCGYC 2 cut(s) 114, 444
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 11, 435, 812
BsaWI WCCGGW 1 cut(s) 733
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 344, 401, 674
Bse1I ACTGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 2 cut(s) 231, 755
BseDI CCNNGG 3 cut(s) 11, 435, 812
BseLI CCNNNNNNNGG 4 cut(s) 230, 344, 401, 674
BseMI GCAATG 1 cut(s) 274
BseMII CTCAG 4 cut(s) 372, 519, 555, 602
BseNI ACTGG 1 cut(s) 67
BseSI GKGCMC 1 cut(s) 701
BseXI GCAGC 6 cut(s) 364, 367, 484, 506, 645, 801
BseYI CCCAGC 1 cut(s) 290
Bsh1236I CGCG 2 cut(s) 195, 437
BshFI GGCC 3 cut(s) 455, 464, 699
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 2 cut(s) 254, 741
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 2 cut(s) 734, 813
BslFI GGGAC 2 cut(s) 301, 744
BslI CCNNNNNNNGG 4 cut(s) 230, 344, 401, 674
BsmAI GTCTC 5 cut(s) 30, 416, 600, 620, 683
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 2 cut(s) 301, 744
BsnI GGCC 3 cut(s) 455, 464, 699
Bso31I GGTCTC 1 cut(s) 600
BsoBI CYCGRG 1 cut(s) 812
Bsp119I TTCGAA 1 cut(s) 819
Bsp120I GGGCCC 1 cut(s) 697
Bsp1286I GDGCHC 3 cut(s) 254, 701, 741
Bsp143I GATC 4 cut(s) 106, 391, 712, 863
Bsp68I TCGCGA 1 cut(s) 195
BspACI CCGC 5 cut(s) 23, 237, 435, 437, 469
BspANI GGCC 3 cut(s) 455, 464, 699
BspCNI CTCAG 4 cut(s) 373, 518, 556, 601
BspFNI CGCG 2 cut(s) 195, 437
BspLI GGNNCC 3 cut(s) 235, 699, 732
BspPI GGATC 2 cut(s) 101, 386
BspQI GCTCTTC 1 cut(s) 648
BspT104I TTCGAA 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 600
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 11, 435, 812
BssMI GATC 4 cut(s) 106, 391, 712, 863
BssNI GRCGYC 2 cut(s) 114, 444
Bst2UI CCWGG 2 cut(s) 231, 755
Bst4CI ACNGT 1 cut(s) 851
Bst6I CTCTTC 4 cut(s) 148, 337, 648, 708
BstACI GRCGYC 2 cut(s) 114, 444
BstBI TTCGAA 1 cut(s) 819
BstDEI CTNAG 4 cut(s) 381, 505, 564, 588
BstDSI CCRYGG 1 cut(s) 435
BstENI CCTNNNNNAGG 2 cut(s) 342, 399
BstFNI CGCG 2 cut(s) 195, 437
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 3 cut(s) 220, 304, 360
BstKTI GATC 4 cut(s) 109, 394, 715, 866
BstMAI GTCTC 5 cut(s) 30, 416, 600, 620, 683
BstMBI GATC 4 cut(s) 106, 391, 712, 863
BstNI CCWGG 2 cut(s) 231, 755
BstSCI CCNGG 4 cut(s) 229, 753, 811, 812
BstSLI GKGCMC 1 cut(s) 701
BstUI CGCG 2 cut(s) 195, 437
BstV1I GCAGC 6 cut(s) 364, 367, 484, 506, 645, 801
BsuRI GGCC 3 cut(s) 455, 464, 699
BtgI CCRYGG 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 840
BtsIMutI CAGTG 1 cut(s) 117
BtuMI TCGCGA 1 cut(s) 195
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 3 cut(s) 220, 304, 360
Cfr13I GGNCC 3 cut(s) 697, 698, 731
Cfr42I CCGCGG 1 cut(s) 438
Cfr9I CCCGGG 1 cut(s) 812
CseI GACGC 2 cut(s) 122, 452
Csp6I GTAC 1 cut(s) 423
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 4 cut(s) 381, 505, 564, 588
DpnI GATC 4 cut(s) 108, 393, 714, 865
DpnII GATC 4 cut(s) 106, 391, 712, 863
Eam1104I CTCTTC 4 cut(s) 148, 337, 648, 708
EarI CTCTTC 4 cut(s) 148, 337, 648, 708
Ecl136II GAGCTC 2 cut(s) 252, 739
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 3 cut(s) 254, 701, 741
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 731
Eco53kI GAGCTC 2 cut(s) 252, 739
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 812
EcoICRI GAGCTC 2 cut(s) 252, 739
EcoNI CCTNNNNNAGG 2 cut(s) 342, 399
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 532
EcoRII CCWGG 2 cut(s) 229, 753
EcoT38I GRGCYC 3 cut(s) 254, 701, 741
Esp3I CGTCTC 1 cut(s) 416
FaiI YATR 3 cut(s) 105, 459, 803
FaqI GGGAC 2 cut(s) 301, 744
FbaI TGATCA 1 cut(s) 712
FblI GTMKAC 2 cut(s) 641, 841
Fnu4HI GCNGC 8 cut(s) 23, 356, 378, 470, 495, 498, 659, 790
FriOI GRGCYC 3 cut(s) 254, 701, 741
Fsp4HI GCNGC 8 cut(s) 23, 356, 378, 470, 495, 498, 659, 790
FspI TGCGCA 1 cut(s) 219
GlaI GCGC 3 cut(s) 219, 303, 359
GluI GCNGC 8 cut(s) 23, 356, 378, 470, 495, 498, 659, 790
GsaI CCCAGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 776
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 3 cut(s) 455, 464, 699
HapII CCGG 2 cut(s) 734, 813
HgaI GACGC 2 cut(s) 122, 452
HhaI GCGC 3 cut(s) 220, 304, 360
Hin1I GRCGYC 2 cut(s) 114, 444
Hin6I GCGC 3 cut(s) 218, 302, 358
HinP1I GCGC 3 cut(s) 218, 302, 358
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HinfI GANTC 9 cut(s) 28, 125, 273, 385, 569, 575, 638, 743, 808
HpaII CCGG 2 cut(s) 734, 813
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 2 cut(s) 642, 842
Hpy188I TCNGA 5 cut(s) 15, 278, 565, 574, 591
Hpy188III TCNNGA 7 cut(s) 110, 194, 260, 389, 650, 683, 716
Hpy8I GTNNAC 2 cut(s) 642, 842
Hpy99I CGWCG 2 cut(s) 116, 551
HpyAV CCTTC 4 cut(s) 140, 292, 609, 616
HpyCH4III ACNGT 1 cut(s) 851
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 1 cut(s) 661
HpyF3I CTNAG 4 cut(s) 381, 505, 564, 588
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 2 cut(s) 114, 444
HspAI GCGC 3 cut(s) 218, 302, 358
Ksp22I TGATCA 1 cut(s) 712
KspI CCGCGG 1 cut(s) 438
Kzo9I GATC 4 cut(s) 106, 391, 712, 863
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 1 cut(s) 736
Lsp1109I GCAGC 6 cut(s) 364, 367, 484, 506, 645, 801
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 2 cut(s) 199, 851
MalI GATC 4 cut(s) 108, 393, 714, 865
MboI GATC 4 cut(s) 106, 391, 712, 863
MboII GAAGA 8 cut(s) 123, 165, 174, 324, 494, 665, 695, 766
MhlI GDGCHC 3 cut(s) 254, 701, 741
MluCI AATT 3 cut(s) 476, 532, 781
MlyI GAGTC 4 cut(s) 119, 282, 569, 647
MmeI TCCRAC 1 cut(s) 549
MspA1I CMGCKG 4 cut(s) 25, 437, 497, 515
MspI CCGG 2 cut(s) 734, 813
MspR9I CCNGG 4 cut(s) 231, 755, 813, 814
MvaI CCWGG 2 cut(s) 231, 755
MvnI CGCG 2 cut(s) 195, 437
NciI CCSGG 2 cut(s) 813, 814
NdeII GATC 4 cut(s) 106, 391, 712, 863
NlaIV GGNNCC 3 cut(s) 235, 699, 732
NmeAIII GCCGAG 2 cut(s) 265, 858
NmuCI GTSAC 1 cut(s) 851
NruI TCGCGA 1 cut(s) 195
NsbI TGCGCA 1 cut(s) 219
NspV TTCGAA 1 cut(s) 819
PceI AGGCCT 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 648
PfeI GAWTC 5 cut(s) 28, 385, 569, 743, 808
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 8 cut(s) 24, 357, 379, 471, 496, 499, 660, 791
PleI GAGTC 4 cut(s) 119, 281, 569, 646
PpsI GAGTC 4 cut(s) 119, 281, 569, 646
Psp124BI GAGCTC 2 cut(s) 254, 741
Psp6I CCWGG 2 cut(s) 229, 753
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 2 cut(s) 229, 753
PspN4I GGNNCC 3 cut(s) 235, 699, 732
PspOMI GGGCCC 1 cut(s) 697
PspPI GGNCC 3 cut(s) 697, 698, 731
PstNI CAGNNNCTG 1 cut(s) 25
PsyI GACNNNGTC 1 cut(s) 578
PvuII CAGCTG 2 cut(s) 497, 515
RruI TCGCGA 1 cut(s) 195
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SacI GAGCTC 2 cut(s) 254, 741
SacII CCGCGG 1 cut(s) 438
SalI GTCGAC 1 cut(s) 640
SapI GCTCTTC 1 cut(s) 648
SatI GCNGC 8 cut(s) 23, 356, 378, 470, 495, 498, 659, 790
Sau3AI GATC 4 cut(s) 106, 391, 712, 863
Sau96I GGNCC 3 cut(s) 697, 698, 731
SchI GAGTC 4 cut(s) 119, 282, 569, 647
ScrFI CCNGG 4 cut(s) 231, 755, 813, 814
SduI GDGCHC 3 cut(s) 254, 701, 741
SfiI GGCCNNNNNGGCC 1 cut(s) 461
Sfr303I CCGCGG 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 819
SgrBI CCGCGG 1 cut(s) 438
SinI GGWCC 1 cut(s) 731
SmaI CCCGGG 1 cut(s) 814
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
Sse9I AATT 3 cut(s) 476, 532, 781
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 5 cut(s) 23, 237, 435, 437, 469
SstI GAGCTC 2 cut(s) 254, 741
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 4 cut(s) 229, 753, 811, 812
TaaI ACNGT 1 cut(s) 851
TaiI ACGT 1 cut(s) 549
TaqI TCGA 6 cut(s) 474, 549, 641, 741, 777, 819
TasI AATT 3 cut(s) 476, 532, 781
TauI GCSGC 2 cut(s) 25, 472
TfiI GAWTC 5 cut(s) 28, 385, 569, 743, 808
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 6 cut(s) 355, 377, 494, 497, 658, 789
Tsp45I GTSAC 1 cut(s) 851
TspDTI ATGAA 2 cut(s) 59, 549
TspMI CCCGGG 1 cut(s) 812
TspRI CASTG 1 cut(s) 124
Tth111I GACNNNGTC 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 731
XagI CCTNNNNNAGG 2 cut(s) 342, 399
XapI RAATTY 2 cut(s) 476, 532
XmaI CCCGGG 1 cut(s) 812
XmiI GTMKAC 2 cut(s) 641, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.