FvH4_4g22080

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001636
Physical Location & Seq
Reverse (-)
25017847 .. 25019337
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22080.t1

Sequence Viewer

Length: 495 bp
ATGCCGGCCGGTCACGGACTCCGATCCCGCACCCGGGACCTCTTTGCCCGCGGCTTCAGGAAGAAAGGTTACATCCCTCTCTCCACCTACCTCAAAACCTACCGGACCGGCCAGTACGTCGACGTCAAGGTCAACGGCGCCGTCCACAAGGGCATGCCCCACAAGTTCTACCACGGGCGGACGGGTCGGGTCTGGAACGTCACCAAACGGGCCATCGGCGTCGAGATCAACAAGCAGGTCGGGAACCGGATCATCCGCAAGCGCATCCAGGTGCGCGTGGAGCACGTGCAGCCGTCGAGATGTGAGGAGGAGTTGAAGGCGAGGAAGGTGAGGAATGATGAGATGAAGGCGGCGGCGAATGCGCGTGGGGAAGTGATCAGCACGAAGAGGCAGCCGGAGGGGCCCAAGCCGGGGTTTATGGTTGAAGGGGCTTCTTTGGAGACTGTCACTCCTATTCCTTATGATGTTGTCAATGACCTCAAGGGAGGGTACTGA

Protein Analysis

165

Amino Acids

18.57

Weight (kDa)

10.39

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21e PF01157 1 - 101 1.1e-44 Ribosomal protein L21e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 137
AccI GTMKAC 1 cut(s) 120
AccII CGCG 3 cut(s) 51, 276, 364
AciI CCGC 7 cut(s) 28, 49, 51, 178, 256, 350, 353
AclWI GGATC 2 cut(s) 18, 257
AcoI YGGCCR 2 cut(s) 6, 109
AcuI CTGAAG 1 cut(s) 40
AcvI CACGTG 1 cut(s) 286
AcyI GRCGYC 3 cut(s) 123, 138, 219
AfaI GTAC 2 cut(s) 116, 491
AfiI CCNNNNNNNGG 4 cut(s) 33, 34, 410, 411
AgsI TTSAA 2 cut(s) 316, 425
AjnI CCWGG 1 cut(s) 267
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 434
AlwI GGATC 2 cut(s) 18, 257
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 4 cut(s) 6, 109, 210, 401
ApaI GGGCCC 1 cut(s) 405
ApeKI GCWGC 2 cut(s) 289, 391
AspLEI GCGC 4 cut(s) 140, 264, 276, 364
AspS9I GGNCC 5 cut(s) 37, 105, 210, 401, 402
AsuC2I CCSGG 3 cut(s) 34, 35, 411
AsuHPI GGTGA 2 cut(s) 193, 340
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 2 cut(s) 37, 105
BaeGI GKGCMC 1 cut(s) 405
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 405
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BbrPI CACGTG 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 301, 403
BccI CCATC 1 cut(s) 221
BceAI ACGGC 3 cut(s) 125, 151, 277
BciT130I CCWGG 1 cut(s) 269
BclI TGATCA 1 cut(s) 375
BcnI CCSGG 3 cut(s) 34, 35, 411
BcoDI GTCTC 1 cut(s) 434
BfoI RGCGCY 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 226
BglI GCCNNNNNGGC 1 cut(s) 400
BisI GCNGC 5 cut(s) 52, 290, 351, 354, 392
BlsI GCNGC 5 cut(s) 53, 291, 352, 355, 393
Bme1390I CCNGG 4 cut(s) 34, 35, 269, 411
Bme18I GGWCC 2 cut(s) 37, 105
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 5 cut(s) 37, 105, 210, 401, 402
BmiI GGNNCC 5 cut(s) 38, 139, 245, 402, 403
BmrFI CCNGG 4 cut(s) 34, 35, 269, 411
BmsI GCATC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 3 cut(s) 34, 35, 411
BsaAI YACGTR 1 cut(s) 286
BsaHI GRCGYC 3 cut(s) 123, 138, 219
BsaJI CCNNGG 4 cut(s) 33, 49, 172, 410
BsaWI WCCGGW 2 cut(s) 102, 246
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 34, 410, 411
Bse118I RCCGGY 3 cut(s) 4, 8, 107
Bse1I ACTGG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 4 cut(s) 33, 49, 172, 410
BseGI GGATG 3 cut(s) 72, 252, 264
BseLI CCNNNNNNNGG 4 cut(s) 33, 34, 410, 411
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 2 cut(s) 320, 323
BseSI GKGCMC 1 cut(s) 405
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 2 cut(s) 301, 403
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 3 cut(s) 51, 276, 364
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 4 cut(s) 8, 111, 212, 403
BshNI GGYRCC 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 8 cut(s) 5, 9, 34, 103, 108, 247, 395, 410
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 4 cut(s) 33, 34, 410, 411
BsmAI GTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 4 cut(s) 8, 111, 212, 403
BsoBI CYCGRG 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 285, 405
Bsp143I GATC 4 cut(s) 23, 225, 249, 375
BspACI CCGC 7 cut(s) 28, 49, 51, 178, 256, 350, 353
BspANI GGCC 4 cut(s) 8, 111, 212, 403
BspFNI CGCG 3 cut(s) 51, 276, 364
BspLI GGNNCC 5 cut(s) 38, 139, 245, 402, 403
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 2 cut(s) 18, 257
BspT107I GGYRCC 1 cut(s) 137
BsrFI RCCGGY 3 cut(s) 4, 8, 107
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 3 cut(s) 4, 8, 107
BssECI CCNNGG 4 cut(s) 33, 49, 172, 410
BssMI GATC 4 cut(s) 23, 225, 249, 375
BssNI GRCGYC 3 cut(s) 123, 138, 219
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 445
Bst6I CTCTTC 1 cut(s) 380
BstACI GRCGYC 3 cut(s) 123, 138, 219
BstBAI YACGTR 1 cut(s) 286
BstC8I GCNNGC 4 cut(s) 6, 49, 155, 260
BstDSI CCRYGG 2 cut(s) 49, 172
BstF5I GGATG 3 cut(s) 72, 252, 264
BstFNI CGCG 3 cut(s) 51, 276, 364
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 4 cut(s) 140, 264, 276, 364
BstKTI GATC 4 cut(s) 26, 228, 252, 378
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 4 cut(s) 23, 225, 249, 375
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 280, 289, 359, 400
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 32, 33, 267, 409
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 3 cut(s) 51, 276, 364
BstV1I GCAGC 2 cut(s) 301, 403
BstZI CGGCCG 1 cut(s) 6
BsuRI GGCC 4 cut(s) 8, 111, 212, 403
BtgI CCRYGG 2 cut(s) 49, 172
BtsCI GGATG 3 cut(s) 72, 252, 264
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 4 cut(s) 6, 49, 155, 260
CfoI GCGC 4 cut(s) 140, 264, 276, 364
Cfr10I RCCGGY 3 cut(s) 4, 8, 107
Cfr13I GGNCC 5 cut(s) 37, 105, 210, 401, 402
Cfr42I CCGCGG 1 cut(s) 52
Cfr9I CCCGGG 1 cut(s) 33
CpoI CGGWCCG 1 cut(s) 105
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 115, 490
CspI CGGWCCG 1 cut(s) 105
CviAII CATG 1 cut(s) 154
CviJI RGCY 9 cut(s) 8, 54, 111, 212, 292, 394, 403, 409, 431
CviKI_1 RGCY 9 cut(s) 8, 54, 111, 212, 292, 394, 403, 409, 431
CviQI GTAC 2 cut(s) 115, 490
DinI GGCGCC 1 cut(s) 139
DpnI GATC 4 cut(s) 25, 227, 251, 377
DpnII GATC 4 cut(s) 23, 225, 249, 375
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 2 cut(s) 6, 109
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
EciI GGCGGA 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 37, 105
Eco52I CGGCCG 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 40
Eco72I CACGTG 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 33
EcoO109I RGGNCCY 2 cut(s) 37, 401
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 405
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
FaeI CATG 1 cut(s) 157
FaiI YATR 3 cut(s) 155, 419, 462
FaqI GGGAC 1 cut(s) 50
FatI CATG 1 cut(s) 153
FauI CCCGC 2 cut(s) 35, 56
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 5 cut(s) 52, 290, 351, 354, 392
FokI GGATG 3 cut(s) 59, 239, 251
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 5 cut(s) 52, 290, 351, 354, 392
GlaI GCGC 4 cut(s) 139, 263, 275, 363
GluI GCNGC 5 cut(s) 52, 290, 351, 354, 392
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 4 cut(s) 8, 111, 212, 403
HapII CCGG 8 cut(s) 5, 9, 34, 103, 108, 247, 395, 410
HgaI GACGC 1 cut(s) 208
HhaI GCGC 4 cut(s) 140, 264, 276, 364
Hin1I GRCGYC 3 cut(s) 123, 138, 219
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 4 cut(s) 138, 262, 274, 362
HinP1I GCGC 4 cut(s) 138, 262, 274, 362
HincII GTYRAC 2 cut(s) 121, 133
HindII GTYRAC 2 cut(s) 121, 133
HinfI GANTC 1 cut(s) 18
HpaII CCGG 8 cut(s) 5, 9, 34, 103, 108, 247, 395, 410
HphI GGTGA 2 cut(s) 193, 340
Hpy166II GTNNAC 3 cut(s) 121, 133, 145
Hpy188I TCNGA 1 cut(s) 23
Hpy188III TCNNGA 5 cut(s) 58, 193, 223, 241, 297
Hpy8I GTNNAC 3 cut(s) 121, 133, 145
Hpy99I CGWCG 4 cut(s) 122, 125, 224, 298
HpyAV CCTTC 4 cut(s) 310, 319, 340, 419
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4IV ACGT 4 cut(s) 117, 123, 198, 285
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 4 cut(s) 280, 289, 359, 400
HpySE526I ACGT 4 cut(s) 117, 123, 198, 285
Hsp92I GRCGYC 3 cut(s) 123, 138, 219
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 4 cut(s) 138, 262, 274, 362
KasI GGCGCC 1 cut(s) 137
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 375
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 4 cut(s) 23, 225, 249, 375
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 2 cut(s) 301, 403
LweI GCATC 1 cut(s) 273
MaeII ACGT 4 cut(s) 117, 123, 198, 285
MaeIII GTNAC 4 cut(s) 11, 68, 199, 445
MalI GATC 4 cut(s) 25, 227, 251, 377
MboI GATC 4 cut(s) 23, 225, 249, 375
MboII GAAGA 2 cut(s) 73, 397
MhlI GDGCHC 2 cut(s) 285, 405
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 1 cut(s) 12
MroNI GCCGGC 1 cut(s) 4
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 8 cut(s) 5, 9, 34, 103, 108, 247, 395, 410
MspR9I CCNGG 4 cut(s) 34, 35, 269, 411
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 3 cut(s) 51, 276, 364
MwoI GCNNNNNNNGC 4 cut(s) 280, 289, 359, 400
NaeI GCCGGC 1 cut(s) 6
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 3 cut(s) 34, 35, 411
NdeII GATC 4 cut(s) 23, 225, 249, 375
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 5 cut(s) 38, 139, 245, 402, 403
NmuCI GTSAC 3 cut(s) 11, 199, 445
NspI RCATGY 1 cut(s) 157
PaeI GCATGC 1 cut(s) 157
PctI GAATGC 1 cut(s) 364
PdiI GCCGGC 1 cut(s) 6
PkrI GCNGC 5 cut(s) 53, 291, 352, 355, 393
PleI GAGTC 1 cut(s) 12
PluTI GGCGCC 1 cut(s) 141
PmaCI CACGTG 1 cut(s) 286
PmlI CACGTG 1 cut(s) 286
PpsI GAGTC 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 267
PspCI CACGTG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 5 cut(s) 38, 139, 245, 402, 403
PspOMI GGGCCC 1 cut(s) 401
PspPI GGNCC 5 cut(s) 37, 105, 210, 401, 402
PspPPI RGGWCCY 1 cut(s) 37
RsaI GTAC 2 cut(s) 116, 491
RsaNI GTAC 2 cut(s) 115, 490
RseI CAYNNNNRTG 1 cut(s) 269
Rsr2I CGGWCCG 1 cut(s) 105
RsrII CGGWCCG 1 cut(s) 105
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 119
SatI GCNGC 5 cut(s) 52, 290, 351, 354, 392
Sau3AI GATC 4 cut(s) 23, 225, 249, 375
Sau96I GGNCC 5 cut(s) 37, 105, 210, 401, 402
SchI GAGTC 1 cut(s) 12
ScrFI CCNGG 4 cut(s) 34, 35, 269, 411
SduI GDGCHC 2 cut(s) 285, 405
SfaNI GCATC 1 cut(s) 273
SfoI GGCGCC 1 cut(s) 139
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 2 cut(s) 37, 105
SmaI CCCGGG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SphI GCATGC 1 cut(s) 157
SsiI CCGC 7 cut(s) 28, 49, 51, 178, 256, 350, 353
SspDI GGCGCC 1 cut(s) 137
StyD4I CCNGG 4 cut(s) 32, 33, 267, 409
TaaI ACNGT 1 cut(s) 445
TaiI ACGT 4 cut(s) 120, 126, 201, 288
TaqI TCGA 3 cut(s) 120, 222, 296
TauI GCSGC 3 cut(s) 54, 353, 356
TseFI GTSAC 3 cut(s) 11, 199, 445
TseI GCWGC 2 cut(s) 289, 391
Tsp45I GTSAC 3 cut(s) 11, 199, 445
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 1 cut(s) 30
TspMI CCCGGG 1 cut(s) 33
VpaK11BI GGWCC 2 cut(s) 37, 105
XceI RCATGY 1 cut(s) 157
XmaI CCCGGG 1 cut(s) 33
XmiI GTMKAC 1 cut(s) 120
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.