MD14G1052100.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001636
Physical Location & Seq
Reverse (-)
4990099 .. 4991694
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1052100.v1.1.491

Sequence Viewer

Length: 495 bp
ATGCCGGCTGGACACGGTCTCCGTTCCCGGACTCGCGATCTGTTCTCGAGGGCCTTCAGGAAGAAGGGGTACATCCCACTCTCCACCTACCTCAAGACCTACAGGATCGGCGACCATGTCGACGTCAAGGTTAACGGCGCCGTCCACAAGGGTATGCCCCACAAGTTCTACCACGGCCGTACCGGTCGCGTCTGGAACGTTACCAAGCGCGCCATCGGTGTCGAGATCAACAAGCAGGTTGGAAACAGAATCATCAGGAAGAGGATCCATGTGCGTGTGGAGCATGTGCAGCCATCAAGGTGCACCGAAGAATTCCGTTTGAGGAAGATTAAGAATGACCAGCTGAAGGCCGAGGCTAAAGCAAAGGGTGAGGTCATTAGCACCAAGAGGCAGCCGAAGGGACCGAAGCCTGGTTTCAGGGTGGAAGGTGCCGTCATGGAAACTGTTACTCCCATTCCTTATGATGTCGTCAACGATCTTAAAGGAGGTTACTAG

Protein Analysis

165

Amino Acids

18.72

Weight (kDa)

10.61

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21e PF01157 1 - 101 2.2e-45 Ribosomal protein L21e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 226
AccB1I GGYRCC 2 cut(s) 137, 428
AccI GTMKAC 1 cut(s) 120
AccII CGCG 3 cut(s) 36, 189, 210
AclI AACGTT 1 cut(s) 198
AclWI GGATC 3 cut(s) 113, 259, 272
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 2 cut(s) 40, 365
AcyI GRCGYC 2 cut(s) 123, 138
AfaI GTAC 2 cut(s) 71, 181
AfiI CCNNNNNNNGG 2 cut(s) 346, 410
AgeI ACCGGT 1 cut(s) 182
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 1 cut(s) 343
AluI AGCT 1 cut(s) 343
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 23
Alw44I GTGCAC 1 cut(s) 301
AlwI GGATC 3 cut(s) 113, 259, 272
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 3 cut(s) 51, 175, 348
ApaLI GTGCAC 1 cut(s) 301
ApeKI GCWGC 2 cut(s) 289, 391
ApoI RAATTY 1 cut(s) 311
AsiGI ACCGGT 1 cut(s) 182
AspLEI GCGC 3 cut(s) 140, 210, 212
AspS9I GGNCC 2 cut(s) 51, 401
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 46
AvaII GGWCC 1 cut(s) 401
BaeGI GKGCMC 1 cut(s) 305
BaeI ACNNNNGTAYC 2 cut(s) 163, 196
BamHI GGATCC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 137, 428
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
Bbv12I GWGCWC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 301, 403
BccI CCATC 2 cut(s) 221, 301
BceAI ACGGC 5 cut(s) 125, 151, 162, 190, 416
BciT130I CCWGG 1 cut(s) 411
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 1 cut(s) 493
BfmI CTRYAG 1 cut(s) 100
BfoI RGCGCY 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 226
BisI GCNGC 2 cut(s) 290, 392
BlsI GCNGC 2 cut(s) 291, 393
Bme1390I CCNGG 2 cut(s) 28, 411
Bme18I GGWCC 1 cut(s) 401
BmeT110I CYCGRG 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 51, 401
BmiI GGNNCC 4 cut(s) 139, 266, 402, 430
BmrFI CCNGG 2 cut(s) 28, 411
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 28
BsaHI GRCGYC 2 cut(s) 123, 138
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 172, 351
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 3 cut(s) 33, 433, 463
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 410
Bse118I RCCGGY 2 cut(s) 4, 182
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 172, 351
BseGI GGATG 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 346, 410
BsePI GCGCGC 1 cut(s) 208
BseSI GKGCMC 1 cut(s) 305
BseX3I CGGCCG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 301, 403
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 3 cut(s) 36, 189, 210
Bsh1285I CGRYCG 2 cut(s) 178, 187
BshFI GGCC 3 cut(s) 53, 177, 350
BshNI GGYRCC 2 cut(s) 137, 428
BshTI ACCGGT 1 cut(s) 182
BsiEI CGRYCG 2 cut(s) 178, 187
BsiHKAI GWGCWC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 3 cut(s) 5, 28, 183
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 2 cut(s) 346, 410
BsmAI GTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 414
BsnI GGCC 3 cut(s) 53, 177, 350
Bso31I GGTCTC 1 cut(s) 23
BsoBI CYCGRG 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 305
Bsp143I GATC 5 cut(s) 37, 105, 225, 264, 475
Bsp68I TCGCGA 1 cut(s) 36
BspANI GGCC 3 cut(s) 53, 177, 350
BspFNI CGCG 3 cut(s) 36, 189, 210
BspLI GGNNCC 4 cut(s) 139, 266, 402, 430
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 3 cut(s) 113, 259, 272
BspT107I GGYRCC 2 cut(s) 137, 428
BspTNI GGTCTC 1 cut(s) 23
BsrFI RCCGGY 2 cut(s) 4, 182
BssAI RCCGGY 2 cut(s) 4, 182
BssECI CCNNGG 2 cut(s) 172, 351
BssHII GCGCGC 1 cut(s) 208
BssMI GATC 5 cut(s) 37, 105, 225, 264, 475
BssNI GRCGYC 2 cut(s) 123, 138
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 17, 445
Bst6I CTCTTC 1 cut(s) 254
BstACI GRCGYC 2 cut(s) 123, 138
BstC8I GCNNGC 2 cut(s) 6, 210
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 72
BstFNI CGCG 3 cut(s) 36, 189, 210
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 3 cut(s) 140, 210, 212
BstKTI GATC 5 cut(s) 40, 108, 228, 267, 478
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 5 cut(s) 37, 105, 225, 264, 475
BstMCI CGRYCG 2 cut(s) 178, 187
BstMWI GCNNNNNNNGC 2 cut(s) 280, 289
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 287
BstSCI CCNGG 2 cut(s) 26, 409
BstSFI CTRYAG 1 cut(s) 100
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 3 cut(s) 36, 189, 210
BstV1I GCAGC 2 cut(s) 301, 403
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
BstZI CGGCCG 1 cut(s) 175
BsuRI GGCC 3 cut(s) 53, 177, 350
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 72
BtuMI TCGCGA 1 cut(s) 36
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 6, 210
CfoI GCGC 3 cut(s) 140, 210, 212
Cfr10I RCCGGY 2 cut(s) 4, 182
Cfr13I GGNCC 2 cut(s) 51, 401
CseI GACGC 1 cut(s) 178
Csp6I GTAC 2 cut(s) 70, 180
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 4 cut(s) 116, 269, 284, 436
CviJI RGCY 9 cut(s) 8, 53, 177, 292, 343, 350, 356, 394, 409
CviKI_1 RGCY 9 cut(s) 8, 53, 177, 292, 343, 350, 356, 394, 409
CviQI GTAC 2 cut(s) 70, 180
DinI GGCGCC 1 cut(s) 139
DpnI GATC 5 cut(s) 39, 107, 227, 266, 477
DpnII GATC 5 cut(s) 37, 105, 225, 264, 475
EaeI YGGCCR 1 cut(s) 175
EagI CGGCCG 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
EclXI CGGCCG 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 401
Eco52I CGGCCG 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 40, 365
Eco88I CYCGRG 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 409
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
FaeI CATG 4 cut(s) 119, 272, 287, 439
FaiI YATR 6 cut(s) 117, 155, 270, 285, 437, 462
FaqI GGGAC 1 cut(s) 414
FatI CATG 4 cut(s) 115, 268, 283, 435
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 290, 392
FokI GGATG 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 290, 392
FspBI CTAG 1 cut(s) 493
GlaI GCGC 3 cut(s) 139, 209, 211
GluI GCNGC 2 cut(s) 290, 392
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 3 cut(s) 53, 177, 350
HapII CCGG 3 cut(s) 5, 28, 183
HgaI GACGC 1 cut(s) 178
HhaI GCGC 3 cut(s) 140, 210, 212
Hin1I GRCGYC 2 cut(s) 123, 138
Hin1II CATG 4 cut(s) 119, 272, 287, 439
Hin6I GCGC 3 cut(s) 138, 208, 210
HinP1I GCGC 3 cut(s) 138, 208, 210
HincII GTYRAC 3 cut(s) 121, 133, 472
HindII GTYRAC 3 cut(s) 121, 133, 472
HinfI GANTC 2 cut(s) 31, 249
HpaI GTTAAC 1 cut(s) 133
HpaII CCGG 3 cut(s) 5, 28, 183
HphI GGTGA 1 cut(s) 380
Hpy166II GTNNAC 5 cut(s) 121, 133, 145, 303, 472
Hpy188III TCNNGA 7 cut(s) 35, 46, 58, 94, 193, 223, 256
Hpy8I GTNNAC 5 cut(s) 121, 133, 145, 303, 472
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 5 cut(s) 58, 64, 340, 391, 419
HpyCH4III ACNGT 2 cut(s) 17, 445
HpyCH4IV ACGT 2 cut(s) 123, 198
HpyCH4V TGCA 2 cut(s) 289, 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 289
HpySE526I ACGT 2 cut(s) 123, 198
Hsp92I GRCGYC 2 cut(s) 123, 138
Hsp92II CATG 4 cut(s) 119, 272, 287, 439
HspAI GCGC 3 cut(s) 138, 208, 210
KasI GGCGCC 1 cut(s) 137
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 133
Kzo9I GATC 5 cut(s) 37, 105, 225, 264, 475
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 2 cut(s) 301, 403
MaeI CTAG 1 cut(s) 493
MaeII ACGT 2 cut(s) 123, 198
MaeIII GTNAC 3 cut(s) 199, 445, 488
MalI GATC 5 cut(s) 39, 107, 227, 266, 477
MboI GATC 5 cut(s) 37, 105, 225, 264, 475
MboII GAAGA 4 cut(s) 73, 271, 320, 337
MflI RGATCY 1 cut(s) 264
MhlI GDGCHC 1 cut(s) 305
MluCI AATT 1 cut(s) 311
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 8 cut(s) 42, 101, 255, 315, 346, 364, 381, 479
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 3 cut(s) 132, 330, 480
MslI CAYNNNNRTG 2 cut(s) 273, 298
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 3 cut(s) 5, 28, 183
MspR9I CCNGG 2 cut(s) 28, 411
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 3 cut(s) 36, 189, 210
MwoI GCNNNNNNNGC 2 cut(s) 280, 289
NaeI GCCGGC 1 cut(s) 6
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 1 cut(s) 28
NdeII GATC 5 cut(s) 37, 105, 225, 264, 475
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 4 cut(s) 119, 272, 287, 439
NlaIV GGNNCC 4 cut(s) 139, 266, 402, 430
NmeAIII GCCGAG 1 cut(s) 376
NruI TCGCGA 1 cut(s) 36
NspI RCATGY 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 46
PauI GCGCGC 1 cut(s) 208
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 1 cut(s) 249
PflFI GACNNNGTC 2 cut(s) 15, 116
PfoI TCCNGGA 1 cut(s) 26
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 2 cut(s) 291, 393
PleI GAGTC 1 cut(s) 25
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 25
Psp1406I AACGTT 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 4 cut(s) 139, 266, 402, 430
PspPI GGNCC 2 cut(s) 51, 401
PsuI RGATCY 1 cut(s) 264
PsyI GACNNNGTC 2 cut(s) 15, 116
PteI GCGCGC 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 343
RruI TCGCGA 1 cut(s) 36
RsaI GTAC 2 cut(s) 71, 181
RsaNI GTAC 2 cut(s) 70, 180
RseI CAYNNNNRTG 2 cut(s) 273, 298
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 3 cut(s) 132, 330, 480
SatI GCNGC 2 cut(s) 290, 392
Sau3AI GATC 5 cut(s) 37, 105, 225, 264, 475
Sau96I GGNCC 2 cut(s) 51, 401
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 28, 411
SduI GDGCHC 1 cut(s) 305
SfcI CTRYAG 1 cut(s) 100
SfoI GGCGCC 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 46
SinI GGWCC 1 cut(s) 401
SlaI CTCGAG 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 273, 298
SmlI CTYRAG 2 cut(s) 46, 92
SmoI CTYRAG 2 cut(s) 46, 92
Sse9I AATT 1 cut(s) 311
SspDI GGCGCC 1 cut(s) 137
SspMI CTAG 1 cut(s) 493
StyD4I CCNGG 2 cut(s) 26, 409
TaaI ACNGT 2 cut(s) 17, 445
TaiI ACGT 2 cut(s) 126, 201
TaqI TCGA 3 cut(s) 47, 120, 222
TaqII GACCGA 1 cut(s) 418
TasI AATT 1 cut(s) 311
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 3 cut(s) 132, 330, 480
Tru9I TTAA 3 cut(s) 132, 330, 480
TseI GCWGC 2 cut(s) 289, 391
TspGWI ACGGA 2 cut(s) 11, 305
Tth111I GACNNNGTC 2 cut(s) 15, 116
VneI GTGCAC 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 1 cut(s) 311
XceI RCATGY 1 cut(s) 287
XhoI CTCGAG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 120
XspI CTAG 1 cut(s) 493
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.