FvH4_4g35841

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003272
Physical Location & Seq
Forward (+)
33011484 .. 33013810
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35841.t1

Sequence Viewer

Length: 960 bp
ATGGCAAAGGTTCCTTCAGTAACCCTAAGCTCCTGCGGTGATGACATCCAGACCATGCCTGTCATCGGCATGGGAACTTCATCGTACCCTCGGGCCGACCCTGAAACCGCCAAGGCTGCCATTCTCGAAGCAATCAGAGCTGGTTACCGACATTTCGACACTGCCGCTGCTTACGGCTCGGAGAAAGATCTCGGTGAAGCCATAGCCGAGGCTCTCCGTCTCCAACTCATCAAGTCTAGGGACGAGCTCTTCATCACAACCAAACTTTGGGCCAGTTTCGCCGAGAAAGACCTTGTGCTGCCCTCCATCAAAGCCAGTTTAAGCAATCTTCAAGCAGAATACATTGACATGTACATCATACACTGGCCGTTCAAATTGGGAAAAGAGGTGAGAACCATGCCTGTTGAGAGAGATCTGGTGCAGCCCCTTGATATCAAATCTGTTTGGGAAGCCATGGAAGAGTGCAAGAAACTTGGGCTTGCTAGAGGTATTGGTGTCAGTAACTTCACTAGCAGCATGCTCGAGGAGCTTCTTTCCTTCGCCGAAACCCCTCCGGCCGTAAACCAATTGGAGATGAACCCAGCTTGGCAGCTGAAGAAATTGAGGGACTTCTGCAAGGCAAAGGGAATTCATGTCACGGCTTACTCTCCGCTCGGAGCAGCTAGGACTAAATGGGGTGACGATAGGGTTTTGGGATCAGATATCATCGAAGAGATTGCCCAAGCCAAAGGAAAATCAACTGCTCAGATATCATTGAGATGGGTGTACGAACAAGGTGTGAGCATAGTAACAAAAAGTTACAACAAAGAAAGAATGAGGCAGAACCTTGACATCTTCGACTTTTGCTTGACCGAGGAGGAACTGGAGAAGATGAGTCATCTTCCACAACGGAAAGGGGTTACATTTGCCTCACTTCTAGGACCCCATGATATCGTTCTGGAAGTTGACGCAGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

320

Amino Acids

35.57

Weight (kDa)

5.45

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 22 - 291 1.7e-54 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 652
AciI CCGC 4 cut(s) 36, 108, 165, 650
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 2 cut(s) 365, 555
AcsI RAATTY 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 3 cut(s) 86, 353, 767
AfiI CCNNNNNNNGG 2 cut(s) 65, 267
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 2 cut(s) 332, 373
AluBI AGCT 7 cut(s) 30, 140, 247, 529, 584, 592, 662
AluI AGCT 7 cut(s) 30, 140, 247, 529, 584, 592, 662
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 1 cut(s) 703
Ama87I CYCGRG 2 cut(s) 90, 521
AoxI GGCC 4 cut(s) 93, 270, 365, 555
ApeKI GCWGC 7 cut(s) 116, 167, 298, 421, 513, 589, 659
ApoI RAATTY 1 cut(s) 627
AspS9I GGNCC 3 cut(s) 93, 270, 920
AsuHPI GGTGA 4 cut(s) 50, 206, 400, 689
AvaI CYCGRG 2 cut(s) 90, 521
AvaII GGWCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 7 cut(s) 103, 154, 285, 433, 525, 601, 671
BccI CCATC 2 cut(s) 314, 753
BceAI ACGGC 4 cut(s) 190, 352, 542, 654
BcgI CGANNNNNNTGC 4 cut(s) 502, 536, 698, 732
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 5 cut(s) 237, 483, 510, 663, 917
BglII AGATCT 2 cut(s) 187, 412
BisI GCNGC 8 cut(s) 117, 165, 168, 299, 422, 514, 590, 660
BlsI GCNGC 8 cut(s) 118, 166, 169, 300, 423, 515, 591, 661
Bme18I GGWCC 1 cut(s) 920
BmeT110I CYCGRG 2 cut(s) 90, 521
BmgT120I GGNCC 3 cut(s) 93, 270, 920
BmiI GGNNCC 2 cut(s) 12, 922
BpmI CTGGAG 1 cut(s) 884
Bpu10I CCTNAGC 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 89, 111, 207, 453, 852
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 267
Bse1I ACTGG 4 cut(s) 273, 315, 368, 867
BseDI CCNNGG 5 cut(s) 89, 111, 207, 453, 852
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 65, 267
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 4 cut(s) 273, 315, 368, 867
BseRI GAGGAG 2 cut(s) 539, 869
BseX3I CGGCCG 1 cut(s) 555
BseXI GCAGC 7 cut(s) 103, 154, 285, 433, 525, 601, 671
BseYI CCCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 440
Bsh1285I CGRYCG 1 cut(s) 558
BshFI GGCC 4 cut(s) 95, 272, 367, 557
BsiEI CGRYCG 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 90, 521
BsiSI CCGG 1 cut(s) 554
BslFI GGGAC 2 cut(s) 254, 620
BslI CCNNNNNNNGG 2 cut(s) 65, 267
BsmAI GTCTC 1 cut(s) 224
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 2 cut(s) 254, 620
BsnI GGCC 4 cut(s) 95, 272, 367, 557
BsoBI CYCGRG 2 cut(s) 90, 521
Bsp1286I GDGCHC 1 cut(s) 249
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 3 cut(s) 187, 412, 695
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 4 cut(s) 36, 108, 165, 650
BspANI GGCC 4 cut(s) 95, 272, 367, 557
BspCNI CTCAG 1 cut(s) 757
BspLI GGNNCC 2 cut(s) 12, 922
BspPI GGATC 1 cut(s) 703
BspQI GCTCTTC 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 652
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 4 cut(s) 273, 315, 368, 867
BssECI CCNNGG 5 cut(s) 89, 111, 207, 453, 852
BssMI GATC 3 cut(s) 187, 412, 695
BssT1I CCWWGG 2 cut(s) 111, 453
Bst6I CTCTTC 3 cut(s) 254, 453, 705
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 480, 518
BstDEI CTNAG 2 cut(s) 26, 744
BstDSI CCRYGG 1 cut(s) 453
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 3 cut(s) 190, 415, 698
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 3 cut(s) 187, 412, 695
BstMCI CGRYCG 1 cut(s) 558
BstMWI GCNNNNNNNGC 4 cut(s) 116, 137, 278, 526
BstNSI RCATGY 2 cut(s) 352, 520
BstPI GGTNACC 1 cut(s) 143
BstV1I GCAGC 7 cut(s) 103, 154, 285, 433, 525, 601, 671
BstX2I RGATCY 2 cut(s) 187, 412
BstYI RGATCY 2 cut(s) 187, 412
BstZI CGGCCG 1 cut(s) 555
BsuRI GGCC 4 cut(s) 95, 272, 367, 557
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 159, 361
Cac8I GCNNGC 2 cut(s) 480, 518
Cfr13I GGNCC 3 cut(s) 93, 270, 920
Csp6I GTAC 3 cut(s) 85, 352, 766
CviAII CATG 8 cut(s) 55, 70, 349, 397, 454, 517, 632, 926
CviQI GTAC 3 cut(s) 85, 352, 766
DdeI CTNAG 2 cut(s) 26, 744
DpnI GATC 3 cut(s) 189, 414, 697
DpnII GATC 3 cut(s) 187, 412, 695
EaeI YGGCCR 2 cut(s) 365, 555
EagI CGGCCG 1 cut(s) 555
Eam1104I CTCTTC 3 cut(s) 254, 453, 705
EarI CTCTTC 3 cut(s) 254, 453, 705
Ecl136II GAGCTC 1 cut(s) 247
EclXI CGGCCG 1 cut(s) 555
Eco130I CCWWGG 2 cut(s) 111, 453
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 4 cut(s) 433, 703, 750, 931
Eco47I GGWCC 1 cut(s) 920
Eco52I CGGCCG 1 cut(s) 555
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 614
Eco88I CYCGRG 2 cut(s) 90, 521
Eco91I GGTNACC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 920
EcoO65I GGTNACC 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 627
EcoRV GATATC 4 cut(s) 433, 703, 750, 931
EcoT14I CCWWGG 2 cut(s) 111, 453
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 2 cut(s) 111, 453
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 8 cut(s) 58, 73, 352, 400, 457, 520, 635, 929
FaqI GGGAC 2 cut(s) 254, 620
FatI CATG 8 cut(s) 54, 69, 348, 396, 453, 516, 631, 925
Fnu4HI GCNGC 8 cut(s) 117, 165, 168, 299, 422, 514, 590, 660
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 8 cut(s) 117, 165, 168, 299, 422, 514, 590, 660
FspBI CTAG 5 cut(s) 237, 483, 510, 663, 917
GluI GCNGC 8 cut(s) 117, 165, 168, 299, 422, 514, 590, 660
GsaI CCCAGC 1 cut(s) 584
GsuI CTGGAG 1 cut(s) 884
HaeIII GGCC 4 cut(s) 95, 272, 367, 557
HapII CCGG 1 cut(s) 554
Hin1II CATG 8 cut(s) 58, 73, 352, 400, 457, 520, 635, 929
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HinfI GANTC 1 cut(s) 874
HpaII CCGG 1 cut(s) 554
HphI GGTGA 4 cut(s) 50, 206, 400, 689
Hpy166II GTNNAC 3 cut(s) 562, 766, 946
Hpy188I TCNGA 5 cut(s) 137, 181, 656, 700, 747
Hpy188III TCNNGA 3 cut(s) 49, 125, 938
Hpy8I GTNNAC 3 cut(s) 562, 766, 946
HpyAV CCTTC 2 cut(s) 24, 547
HpyCH4V TGCA 3 cut(s) 421, 465, 615
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 137, 278, 526
HpyF3I CTNAG 2 cut(s) 26, 744
Hsp92II CATG 8 cut(s) 58, 73, 352, 400, 457, 520, 635, 929
Kzo9I GATC 3 cut(s) 187, 412, 695
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 3 cut(s) 35, 526, 656
Lsp1109I GCAGC 7 cut(s) 103, 154, 285, 433, 525, 601, 671
MaeI CTAG 5 cut(s) 237, 483, 510, 663, 917
MaeIII GTNAC 8 cut(s) 19, 143, 500, 634, 677, 787, 797, 898
MalI GATC 3 cut(s) 189, 414, 697
MbiI CCGCTC 1 cut(s) 652
MboI GATC 3 cut(s) 187, 412, 695
MboII GAAGA 8 cut(s) 241, 320, 470, 607, 722, 826, 872, 880
MfeI CAATTG 1 cut(s) 566
MflI RGATCY 2 cut(s) 187, 412
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 5 cut(s) 374, 566, 599, 627, 953
MlyI GAGTC 1 cut(s) 883
MmeI TCCRAC 1 cut(s) 247
MseI TTAA 1 cut(s) 320
MslI CAYNNNNRTG 3 cut(s) 68, 347, 757
MspA1I CMGCKG 2 cut(s) 167, 592
MspI CCGG 1 cut(s) 554
MunI CAATTG 1 cut(s) 566
MwoI GCNNNNNNNGC 4 cut(s) 116, 137, 278, 526
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 187, 412, 695
NlaIII CATG 8 cut(s) 58, 73, 352, 400, 457, 520, 635, 929
NlaIV GGNNCC 2 cut(s) 12, 922
NmeAIII GCCGAG 2 cut(s) 232, 307
NmuCI GTSAC 2 cut(s) 634, 677
NspI RCATGY 2 cut(s) 352, 520
PaeI GCATGC 1 cut(s) 520
PaeR7I CTCGAG 1 cut(s) 521
PciI ACATGT 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 254
PflMI CCANNNNNTGG 1 cut(s) 267
PkrI GCNGC 8 cut(s) 118, 166, 169, 300, 423, 515, 591, 661
PleI GAGTC 1 cut(s) 882
PpsI GAGTC 1 cut(s) 882
PpuMI RGGWCCY 1 cut(s) 920
PscI ACATGT 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 920
PspEI GGTNACC 1 cut(s) 143
PspFI CCCAGC 1 cut(s) 580
PspN4I GGNNCC 2 cut(s) 12, 922
PspPI GGNCC 3 cut(s) 93, 270, 920
PspPPI RGGWCCY 1 cut(s) 920
PspXI VCTCGAGB 1 cut(s) 521
PsuI RGATCY 2 cut(s) 187, 412
PvuII CAGCTG 1 cut(s) 592
RsaI GTAC 3 cut(s) 86, 353, 767
RsaNI GTAC 3 cut(s) 85, 352, 766
RseI CAYNNNNRTG 3 cut(s) 68, 347, 757
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 8 cut(s) 117, 165, 168, 299, 422, 514, 590, 660
Sau3AI GATC 3 cut(s) 187, 412, 695
Sau96I GGNCC 3 cut(s) 93, 270, 920
SchI GAGTC 1 cut(s) 883
SduI GDGCHC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 521
SinI GGWCC 1 cut(s) 920
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 3 cut(s) 68, 347, 757
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SphI GCATGC 1 cut(s) 520
Sse9I AATT 5 cut(s) 374, 566, 599, 627, 953
SsiI CCGC 4 cut(s) 36, 108, 165, 650
SspMI CTAG 5 cut(s) 237, 483, 510, 663, 917
SstI GAGCTC 1 cut(s) 249
StyI CCWWGG 2 cut(s) 111, 453
TaqI TCGA 5 cut(s) 126, 156, 522, 708, 837
TaqII GACCGA 1 cut(s) 866
TasI AATT 5 cut(s) 374, 566, 599, 627, 953
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 2 cut(s) 166, 368
TseFI GTSAC 2 cut(s) 634, 677
TseI GCWGC 7 cut(s) 116, 167, 298, 421, 513, 589, 659
Tsp45I GTSAC 2 cut(s) 634, 677
TspDTI ATGAA 4 cut(s) 69, 241, 590, 620
TspGWI ACGGA 2 cut(s) 206, 904
TspRI CASTG 2 cut(s) 166, 368
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 920
XapI RAATTY 1 cut(s) 627
XceI RCATGY 2 cut(s) 352, 520
XhoI CTCGAG 1 cut(s) 521
XspI CTAG 5 cut(s) 237, 483, 510, 663, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.