pycom16g01130

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003272
Physical Location & Seq
Reverse (-)
691744 .. 693168
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01130.1

Sequence Viewer

Length: 801 bp
ATGACGCCAACGATAGTTCCTGAGGTAACACTGAGCTCTTGTGACATGACGATGCCAGTGATCGGCATGGGGACTTCATCATACCCTAAAGTTGACCCTGAAACAACCAAGGCTGCAATTCTTGAAGCCATCAGAGCGGGCTACCGCCACTTCGACACGGCCTTCATTTACGGGTCAGAGCAAGATCTCGGGGAAGCCATAGCTGAAGCACTCCGTCTTGGACTCGTCAAGTCTAGGAGTGAGCTTTTCATCACCACTAAGCTTTTTGCCAGCTTTGCTGAAAGAGACCTTGTAGTGCCTGCCATCAACATGAGTTTAAGGAATCTGCAATTGGAGTATGTGGATATGTACCTAATACATTGGCCCTTCAAATTTGGGAAAATGGTGAGCTCTATGCCAGTGGAAAAGGGGGTGTTGCTACCCTTAGATATCAAGTCGGTTTGGGAAGGCATGGAAGAATGCAAGAGACTGGGCCTTGCGAGGGGCATTGGCGTGAGTAACTTCACTTCCGAGATGCTTGAGGACCTCCTTTCGATCGCCAATATCCCTCCCGCCGTCAACCAATTGGAGGTGAACCCGCTTTGGCAGCTGAAGAAACTGAGGGAGTTCTGTAAGGCAAAGGGCATTCATGTGACGGCCTACTCCCCGTTAGGTGCATCCGGGACTAGATATGGAGAAAACAAAGTTTTAGGCACAAAAGTCCTTCAAGACATTGCCAGGTCCAAAGGAAAAACAACTGCAGATAGCGTTGAGATGGGTGTACCAACAAGGGGTGAGCTTGATAACAAAGAGCTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

267

Amino Acids

29.35

Weight (kDa)

6.02

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 223 2.3e-42 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 137
AciI CCGC 4 cut(s) 137, 145, 552, 578
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 2 cut(s) 225, 611
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 350, 762
AfiI CCNNNNNNNGG 4 cut(s) 62, 481, 568, 770
AgsI TTSAA 4 cut(s) 125, 370, 707, 797
AjnI CCWGG 1 cut(s) 716
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AluBI AGCT 9 cut(s) 36, 203, 244, 262, 273, 390, 589, 778, 793
AluI AGCT 9 cut(s) 36, 203, 244, 262, 273, 390, 589, 778, 793
Alw21I GWGCWC 2 cut(s) 38, 392
Alw26I GTCTC 2 cut(s) 279, 460
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 4 cut(s) 159, 362, 472, 636
ApeKI GCWGC 2 cut(s) 113, 586
ApoI RAATTY 1 cut(s) 371
AspS9I GGNCC 4 cut(s) 363, 472, 523, 720
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 4 cut(s) 244, 397, 583, 785
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 523, 720
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 38, 392
BarI GAAGNNNNNNTAC 2 cut(s) 490, 522
Bbv12I GWGCWC 2 cut(s) 38, 392
BbvI GCAGC 2 cut(s) 100, 598
BccI CCATC 3 cut(s) 137, 311, 748
BceAI ACGGC 3 cut(s) 174, 539, 651
BciT130I CCWGG 1 cut(s) 718
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 279, 460
BfaI CTAG 2 cut(s) 234, 666
BfmI CTRYAG 1 cut(s) 738
BglII AGATCT 1 cut(s) 184
BisI GCNGC 2 cut(s) 114, 587
BlsI GCNGC 2 cut(s) 115, 588
Bme1390I CCNGG 2 cut(s) 661, 718
Bme18I GGWCC 2 cut(s) 523, 720
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 4 cut(s) 363, 472, 523, 720
BmrFI CCNGG 2 cut(s) 661, 718
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 3 cut(s) 42, 504, 665
BmuI ACTGGG 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 481, 568, 770
Bse1I ACTGG 3 cut(s) 56, 398, 474
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 711
BseBI CCWGG 1 cut(s) 718
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 656
BseLI CCNNNNNNNGG 4 cut(s) 62, 481, 568, 770
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 3 cut(s) 12, 23, 590
BseNI ACTGG 3 cut(s) 56, 398, 474
BseXI GCAGC 2 cut(s) 100, 598
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 4 cut(s) 161, 364, 474, 638
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 38, 392
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 2 cut(s) 85, 676
BslI CCNNNNNNNGG 4 cut(s) 62, 481, 568, 770
BsmAI GTCTC 2 cut(s) 279, 460
BsmFI GGGAC 2 cut(s) 85, 676
BsmI GAATGC 2 cut(s) 464, 624
BsnI GGCC 4 cut(s) 161, 364, 474, 638
Bso31I GGTCTC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 38, 392
Bsp143I GATC 3 cut(s) 60, 184, 534
BspACI CCGC 4 cut(s) 137, 145, 552, 578
BspANI GGCC 4 cut(s) 161, 364, 474, 638
BspCNI CTCAG 3 cut(s) 13, 24, 591
BspMAI CTGCAG 1 cut(s) 742
BspTNI GGTCTC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 3 cut(s) 56, 398, 474
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 3 cut(s) 60, 184, 534
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 718
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 139, 271, 300
BstDEI CTNAG 5 cut(s) 21, 32, 258, 424, 599
BstF5I GGATG 1 cut(s) 656
BstKTI GATC 3 cut(s) 63, 187, 537
BstMAI GTCTC 2 cut(s) 279, 460
BstMBI GATC 3 cut(s) 60, 184, 534
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 3 cut(s) 134, 275, 586
BstNI CCWGG 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 659, 716
BstSFI CTRYAG 1 cut(s) 738
BstV1I GCAGC 2 cut(s) 100, 598
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 4 cut(s) 161, 364, 474, 638
BtsCI GGATG 1 cut(s) 656
BtsIMutI CAGTG 3 cut(s) 29, 63, 405
Cac8I GCNNGC 3 cut(s) 139, 271, 300
Cfr13I GGNCC 4 cut(s) 363, 472, 523, 720
CseI GACGC 1 cut(s) 13
Csp6I GTAC 2 cut(s) 349, 761
CviAII CATG 5 cut(s) 46, 67, 310, 451, 629
CviQI GTAC 2 cut(s) 349, 761
DdeI CTNAG 5 cut(s) 21, 32, 258, 424, 599
DpnI GATC 3 cut(s) 62, 186, 536
DpnII GATC 3 cut(s) 60, 184, 534
Ecl136II GAGCTC 2 cut(s) 36, 390
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 2 cut(s) 38, 392
Eco31I GGTCTC 1 cut(s) 279
Eco32I GATATC 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 523, 720
Eco53kI GAGCTC 2 cut(s) 36, 390
Eco57I CTGAAG 2 cut(s) 225, 611
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 2 cut(s) 36, 390
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 716
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 2 cut(s) 38, 392
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 5 cut(s) 49, 70, 313, 454, 632
FaqI GGGAC 2 cut(s) 85, 676
FatI CATG 5 cut(s) 45, 66, 309, 450, 628
FauI CCCGC 3 cut(s) 130, 559, 585
Fnu4HI GCNGC 2 cut(s) 114, 587
FokI GGATG 1 cut(s) 643
FriOI GRGCYC 2 cut(s) 38, 392
Fsp4HI GCNGC 2 cut(s) 114, 587
FspBI CTAG 2 cut(s) 234, 666
GluI GCNGC 2 cut(s) 114, 587
HaeIII GGCC 4 cut(s) 161, 364, 474, 638
HapII CCGG 1 cut(s) 660
HgaI GACGC 1 cut(s) 13
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 49, 70, 313, 454, 632
HincII GTYRAC 2 cut(s) 94, 559
HindII GTYRAC 2 cut(s) 94, 559
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 2 cut(s) 222, 322
HpaII CCGG 1 cut(s) 660
HphI GGTGA 4 cut(s) 244, 397, 583, 785
Hpy166II GTNNAC 4 cut(s) 94, 559, 574, 761
Hpy188I TCNGA 3 cut(s) 134, 178, 511
Hpy188III TCNNGA 3 cut(s) 20, 122, 707
Hpy8I GTNNAC 4 cut(s) 94, 559, 574, 761
HpyAV CCTTC 4 cut(s) 172, 376, 440, 713
HpyCH4V TGCA 5 cut(s) 116, 328, 462, 656, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 275, 586
HpyF3I CTNAG 5 cut(s) 21, 32, 258, 424, 599
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 49, 70, 313, 454, 632
Kzo9I GATC 3 cut(s) 60, 184, 534
Lsp1109I GCAGC 2 cut(s) 100, 598
LweI GCATC 3 cut(s) 42, 504, 665
MaeI CTAG 2 cut(s) 234, 666
MaeIII GTNAC 4 cut(s) 25, 41, 497, 631
MalI GATC 3 cut(s) 62, 186, 536
MbiI CCGCTC 1 cut(s) 137
MboI GATC 3 cut(s) 60, 184, 534
MboII GAAGA 2 cut(s) 467, 604
MfeI CAATTG 2 cut(s) 329, 563
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 38, 392
MluCI AATT 4 cut(s) 117, 329, 371, 563
MlyI GAGTC 1 cut(s) 216
MnlI CCTC 7 cut(s) 16, 474, 514, 536, 558, 562, 594
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 4 cut(s) 50, 308, 491, 629
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 2 cut(s) 661, 718
MunI CAATTG 2 cut(s) 329, 563
Mva1269I GAATGC 2 cut(s) 464, 624
MvaI CCWGG 1 cut(s) 718
MwoI GCNNNNNNNGC 3 cut(s) 134, 275, 586
NciI CCSGG 1 cut(s) 661
NdeII GATC 3 cut(s) 60, 184, 534
NlaIII CATG 5 cut(s) 49, 70, 313, 454, 632
NmuCI GTSAC 2 cut(s) 41, 631
PctI GAATGC 2 cut(s) 464, 624
PfeI GAWTC 1 cut(s) 322
PfoI TCCNGGA 1 cut(s) 659
PkrI GCNGC 2 cut(s) 115, 588
Ple19I CGATCG 1 cut(s) 537
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PpuMI RGGWCCY 1 cut(s) 523
Psp124BI GAGCTC 2 cut(s) 38, 392
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 716
PspGI CCWGG 1 cut(s) 716
PspPI GGNCC 4 cut(s) 363, 472, 523, 720
PspPPI RGGWCCY 1 cut(s) 523
PstI CTGCAG 1 cut(s) 742
PsuI RGATCY 1 cut(s) 184
PvuI CGATCG 1 cut(s) 537
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 2 cut(s) 350, 762
RsaNI GTAC 2 cut(s) 349, 761
RseI CAYNNNNRTG 4 cut(s) 50, 308, 491, 629
SacI GAGCTC 2 cut(s) 38, 392
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 2 cut(s) 114, 587
Sau3AI GATC 3 cut(s) 60, 184, 534
Sau96I GGNCC 4 cut(s) 363, 472, 523, 720
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 2 cut(s) 661, 718
SduI GDGCHC 2 cut(s) 38, 392
SfaNI GCATC 3 cut(s) 42, 504, 665
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 2 cut(s) 523, 720
SmiMI CAYNNNNRTG 4 cut(s) 50, 308, 491, 629
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 4 cut(s) 117, 329, 371, 563
SsiI CCGC 4 cut(s) 137, 145, 552, 578
SspMI CTAG 2 cut(s) 234, 666
SstI GAGCTC 2 cut(s) 38, 392
StyD4I CCNGG 2 cut(s) 659, 716
StyI CCWWGG 1 cut(s) 108
TaqI TCGA 2 cut(s) 153, 533
TasI AATT 4 cut(s) 117, 329, 371, 563
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 3 cut(s) 36, 63, 405
TseFI GTSAC 2 cut(s) 41, 631
TseI GCWGC 2 cut(s) 113, 586
Tsp45I GTSAC 2 cut(s) 41, 631
TspDTI ATGAA 4 cut(s) 66, 154, 238, 617
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 3 cut(s) 36, 63, 405
VpaK11BI GGWCC 2 cut(s) 523, 720
XapI RAATTY 1 cut(s) 371
XspI CTAG 2 cut(s) 234, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.