MD13G1014600.v1.1

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003272
Physical Location & Seq
Reverse (-)
911301 .. 913289
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1014600.v1.1.491

Sequence Viewer

Length: 957 bp
ATGACGCCAATTATAGTTCCTGAAGTAACGTTGATCTCCTGTGACATGACGATGCCAGTGATCGGCATGGGGACTTCACCGTACCCTAAAGTCGACCCTGAAACAGCCATAGCTGCAATTCTTGAAGCAATCAGAGCGGGCTACCGCCACTTCGACACCGCCTTTGTTTATGGGTCAGAGCGAGATCTGGGGGGAGCCATAGCTGAAGCACTGCGTCTTGGACTTGTCAAGTCCAGGAGTGAGCTCTTCATCACCACCAAGCTTTCTGCCAGCTTTGCTGAAAGAGACCTTGTAGTGCCTGCCATCAACATGAGTTTAAGGAATCTGCAATTGGAGTACGTGGACATGTACATAATACATTGGCCCTTCAAACTGGGGAAAGTGGTGAGCTCTATGCCAGTAGAAAAGGGAGTGCTGCTACCCTTAGATATCAAGTCAGTTTGGGAAGGCATGGAAGAATGCAAGAGACTGGGCCTTGCGAGGGGCATTGGCGTCAGTAACTTCACTTCCGAGATGCTTGAAGACCTCCTTTCCATCGCCAAAATCCCTCCCGCCCTCAACCAATTGGAGATGAACCCACTTTGGCAGCTGAAGGAACTGAGGGAGTTCTGTAAGGCGAAGGGCATTCATGTGACGGCCTACTCTCCGTTAGGGTCAACCCCGTCTAGATTTGGAGACAACAAAGTCTTAGGCTCAAAAGTTCTCCAGGACATGGCCAAAGCCAAAGGAAAAACAACTGCTCAGATAGCGTTGAGATGGGTGTACCAGCAGGGGGTGAGCTTGGTAACAAAGAGTTCCAACAAGGAAAGAATGAATCAGAACCTTGACATCTTCAGTTGGTCCTTGACTGAGGAGGAATTAGACAAGATTAGTCGTCTTCCTCAACACAAAACAATCACCTTCGCCTCAATTATGGGACCGCATGATGTTGTGCTCCGGATTGATGCTGGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

319

Amino Acids

35.25

Weight (kDa)

7.69

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 292 4.1e-53 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 683
AccB7I CCANNNNNTGG 1 cut(s) 712
AccBSI CCGCTC 1 cut(s) 137
AccI GTMKAC 1 cut(s) 93
AccIII TCCGGA 1 cut(s) 936
AciI CCGC 5 cut(s) 137, 145, 159, 552, 920
AclI AACGTT 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 714
AcuI CTGAAG 4 cut(s) 42, 225, 611, 817
AcyI GRCGYC 2 cut(s) 5, 492
AfaI GTAC 4 cut(s) 83, 338, 350, 764
AfiI CCNNNNNNNGG 4 cut(s) 62, 481, 712, 772
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 3 cut(s) 125, 370, 521
AjnI CCWGG 2 cut(s) 233, 705
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AluBI AGCT 8 cut(s) 113, 203, 244, 262, 273, 390, 589, 780
AluI AGCT 8 cut(s) 113, 203, 244, 262, 273, 390, 589, 780
Alw21I GWGCWC 3 cut(s) 246, 392, 936
Alw26I GTCTC 3 cut(s) 279, 460, 669
Aor13HI TCCGGA 1 cut(s) 936
AoxI GGCC 4 cut(s) 362, 472, 636, 714
ApeKI GCWGC 3 cut(s) 113, 415, 586
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspS9I GGNCC 4 cut(s) 363, 472, 840, 917
AsuHPI GGTGA 5 cut(s) 69, 244, 397, 787, 889
AvaII GGWCC 2 cut(s) 840, 917
BalI TGGCCA 1 cut(s) 716
BanII GRGCYC 2 cut(s) 246, 392
BarI GAAGNNNNNNTAC 2 cut(s) 490, 522
BbsI GAAGAC 2 cut(s) 528, 869
Bbv12I GWGCWC 3 cut(s) 246, 392, 936
BbvI GCAGC 3 cut(s) 100, 402, 598
BccI CCATC 3 cut(s) 311, 542, 750
BceAI ACGGC 1 cut(s) 651
BciT130I CCWGG 2 cut(s) 235, 707
BcoDI GTCTC 3 cut(s) 279, 460, 669
BfaI CTAG 1 cut(s) 666
BglII AGATCT 1 cut(s) 184
BisI GCNGC 3 cut(s) 114, 416, 587
BlsI GCNGC 3 cut(s) 115, 417, 588
Bme1390I CCNGG 2 cut(s) 235, 707
Bme18I GGWCC 2 cut(s) 840, 917
BmgT120I GGNCC 4 cut(s) 363, 472, 840, 917
BmiI GGNNCC 2 cut(s) 196, 918
BmrFI CCNGG 2 cut(s) 235, 707
BmrI ACTGGG 2 cut(s) 383, 479
BmsI GCATC 3 cut(s) 42, 504, 934
BmuI ACTGGG 2 cut(s) 383, 479
BpiI GAAGAC 2 cut(s) 528, 869
BpmI CTGGAG 1 cut(s) 689
BsaAI YACGTR 1 cut(s) 340
BsaHI GRCGYC 2 cut(s) 5, 492
BsaI GGTCTC 1 cut(s) 279
BsaWI WCCGGW 1 cut(s) 936
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 481, 712, 772
Bse1I ACTGG 4 cut(s) 56, 378, 398, 474
BseAI TCCGGA 1 cut(s) 936
BseBI CCWGG 2 cut(s) 235, 707
BseLI CCNNNNNNNGG 4 cut(s) 62, 481, 712, 772
BseMII CTCAG 3 cut(s) 590, 755, 840
BseNI ACTGG 4 cut(s) 56, 378, 398, 474
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 100, 402, 598
BshFI GGCC 4 cut(s) 364, 474, 638, 716
BsiHKAI GWGCWC 3 cut(s) 246, 392, 936
BsiSI CCGG 1 cut(s) 937
BslFI GGGAC 2 cut(s) 85, 930
BslI CCNNNNNNNGG 4 cut(s) 62, 481, 712, 772
BsmAI GTCTC 3 cut(s) 279, 460, 669
BsmFI GGGAC 2 cut(s) 85, 930
BsmI GAATGC 2 cut(s) 464, 624
BsnI GGCC 4 cut(s) 364, 474, 638, 716
Bso31I GGTCTC 1 cut(s) 279
Bsp1286I GDGCHC 3 cut(s) 246, 392, 936
Bsp13I TCCGGA 1 cut(s) 936
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 3 cut(s) 33, 60, 184
BspACI CCGC 5 cut(s) 137, 145, 159, 552, 920
BspANI GGCC 4 cut(s) 364, 474, 638, 716
BspCNI CTCAG 3 cut(s) 591, 754, 841
BspEI TCCGGA 1 cut(s) 936
BspLI GGNNCC 2 cut(s) 196, 918
BspQI GCTCTTC 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 4 cut(s) 56, 378, 398, 474
BssMI GATC 3 cut(s) 33, 60, 184
BssNI GRCGYC 2 cut(s) 5, 492
Bst2UI CCWGG 2 cut(s) 235, 707
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 2 cut(s) 5, 492
BstAUI TGTACA 1 cut(s) 348
BstBAI YACGTR 1 cut(s) 340
BstC8I GCNNGC 3 cut(s) 139, 271, 300
BstDEI CTNAG 5 cut(s) 424, 599, 688, 741, 849
BstKTI GATC 3 cut(s) 36, 63, 187
BstMAI GTCTC 3 cut(s) 279, 460, 669
BstMBI GATC 3 cut(s) 33, 60, 184
BstMWI GCNNNNNNNGC 4 cut(s) 113, 134, 275, 746
BstNI CCWGG 2 cut(s) 235, 707
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 233, 705
BstV1I GCAGC 3 cut(s) 100, 402, 598
BstV2I GAAGAC 2 cut(s) 528, 869
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 4 cut(s) 364, 474, 638, 716
BtgZI GCGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 209
BtsIMutI CAGTG 2 cut(s) 63, 209
Cac8I GCNNGC 3 cut(s) 139, 271, 300
Cfr13I GGNCC 4 cut(s) 363, 472, 840, 917
CseI GACGC 3 cut(s) 13, 203, 481
Csp6I GTAC 4 cut(s) 82, 337, 349, 763
CviAII CATG 8 cut(s) 46, 67, 310, 346, 451, 629, 712, 923
CviQI GTAC 4 cut(s) 82, 337, 349, 763
DdeI CTNAG 5 cut(s) 424, 599, 688, 741, 849
DpnI GATC 3 cut(s) 35, 62, 186
DpnII GATC 3 cut(s) 33, 60, 184
DrdI GACNNNNNNGTC 1 cut(s) 683
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 1 cut(s) 714
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Ecl136II GAGCTC 2 cut(s) 244, 390
Eco24I GRGCYC 2 cut(s) 246, 392
Eco31I GGTCTC 1 cut(s) 279
Eco32I GATATC 1 cut(s) 430
Eco47I GGWCC 2 cut(s) 840, 917
Eco53kI GAGCTC 2 cut(s) 244, 390
Eco57I CTGAAG 4 cut(s) 42, 225, 611, 817
EcoICRI GAGCTC 2 cut(s) 244, 390
EcoRII CCWGG 2 cut(s) 233, 705
EcoRV GATATC 1 cut(s) 430
EcoT38I GRGCYC 2 cut(s) 246, 392
FaeI CATG 8 cut(s) 49, 70, 313, 349, 454, 632, 715, 926
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 2 cut(s) 85, 930
FatI CATG 8 cut(s) 45, 66, 309, 345, 450, 628, 711, 922
FauI CCCGC 2 cut(s) 130, 559
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 114, 416, 587
FriOI GRGCYC 2 cut(s) 246, 392
Fsp4HI GCNGC 3 cut(s) 114, 416, 587
FspBI CTAG 1 cut(s) 666
GluI GCNGC 3 cut(s) 114, 416, 587
GsuI CTGGAG 1 cut(s) 689
HaeIII GGCC 4 cut(s) 364, 474, 638, 716
HapII CCGG 1 cut(s) 937
HgaI GACGC 3 cut(s) 13, 203, 481
Hin1I GRCGYC 2 cut(s) 5, 492
Hin1II CATG 8 cut(s) 49, 70, 313, 349, 454, 632, 715, 926
HincII GTYRAC 2 cut(s) 94, 657
HindII GTYRAC 2 cut(s) 94, 657
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 2 cut(s) 322, 814
HpaII CCGG 1 cut(s) 937
HphI GGTGA 5 cut(s) 69, 244, 397, 787, 889
Hpy166II GTNNAC 4 cut(s) 94, 343, 657, 763
Hpy188I TCNGA 5 cut(s) 134, 178, 511, 744, 819
Hpy188III TCNNGA 4 cut(s) 20, 122, 666, 937
Hpy8I GTNNAC 4 cut(s) 94, 343, 657, 763
HpyAV CCTTC 5 cut(s) 376, 440, 586, 613, 910
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4IV ACGT 2 cut(s) 29, 339
HpyCH4V TGCA 3 cut(s) 116, 328, 462
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 134, 275, 746
HpyF3I CTNAG 5 cut(s) 424, 599, 688, 741, 849
HpySE526I ACGT 2 cut(s) 29, 339
Hsp92I GRCGYC 2 cut(s) 5, 492
Hsp92II CATG 8 cut(s) 49, 70, 313, 349, 454, 632, 715, 926
Kpn2I TCCGGA 1 cut(s) 936
Kzo9I GATC 3 cut(s) 33, 60, 184
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 2 cut(s) 194, 939
Lsp1109I GCAGC 3 cut(s) 100, 402, 598
LweI GCATC 3 cut(s) 42, 504, 934
MaeI CTAG 1 cut(s) 666
MaeII ACGT 2 cut(s) 29, 339
MaeIII GTNAC 5 cut(s) 25, 41, 497, 631, 784
MalI GATC 3 cut(s) 35, 62, 186
MbiI CCGCTC 1 cut(s) 137
MboI GATC 3 cut(s) 33, 60, 184
MboII GAAGA 5 cut(s) 238, 467, 533, 823, 869
MfeI CAATTG 2 cut(s) 329, 563
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 3 cut(s) 246, 392, 936
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 6 cut(s) 9, 117, 329, 563, 857, 909
MluNI TGGCCA 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 822
MnlI CCTC 9 cut(s) 474, 536, 558, 566, 594, 844, 847, 891, 916
Mox20I TGGCCA 1 cut(s) 716
MroI TCCGGA 1 cut(s) 936
MscI TGGCCA 1 cut(s) 716
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 3 cut(s) 50, 308, 629
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 1 cut(s) 937
MspR9I CCNGG 2 cut(s) 235, 707
MunI CAATTG 2 cut(s) 329, 563
Mva1269I GAATGC 2 cut(s) 464, 624
MvaI CCWGG 2 cut(s) 235, 707
MwoI GCNNNNNNNGC 4 cut(s) 113, 134, 275, 746
NdeII GATC 3 cut(s) 33, 60, 184
NlaIII CATG 8 cut(s) 49, 70, 313, 349, 454, 632, 715, 926
NlaIV GGNNCC 2 cut(s) 196, 918
NmuCI GTSAC 2 cut(s) 41, 631
NspI RCATGY 1 cut(s) 349
PciI ACATGT 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 2 cut(s) 464, 624
PfeI GAWTC 2 cut(s) 322, 814
PflMI CCANNNNNTGG 1 cut(s) 712
PfoI TCCNGGA 2 cut(s) 233, 705
PkrI GCNGC 3 cut(s) 115, 417, 588
Ppu21I YACGTR 1 cut(s) 340
PscI ACATGT 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 246, 392
Psp1406I AACGTT 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 233, 705
PspGI CCWGG 2 cut(s) 233, 705
PspN4I GGNNCC 2 cut(s) 196, 918
PspPI GGNCC 4 cut(s) 363, 472, 840, 917
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 4 cut(s) 83, 338, 350, 764
RsaNI GTAC 4 cut(s) 82, 337, 349, 763
RseI CAYNNNNRTG 3 cut(s) 50, 308, 629
SacI GAGCTC 2 cut(s) 246, 392
SalI GTCGAC 1 cut(s) 92
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 114, 416, 587
Sau3AI GATC 3 cut(s) 33, 60, 184
Sau96I GGNCC 4 cut(s) 363, 472, 840, 917
ScrFI CCNGG 2 cut(s) 235, 707
SduI GDGCHC 3 cut(s) 246, 392, 936
SfaNI GCATC 3 cut(s) 42, 504, 934
SinI GGWCC 2 cut(s) 840, 917
SmiMI CAYNNNNRTG 3 cut(s) 50, 308, 629
Sse9I AATT 6 cut(s) 9, 117, 329, 563, 857, 909
SsiI CCGC 5 cut(s) 137, 145, 159, 552, 920
SspMI CTAG 1 cut(s) 666
SstI GAGCTC 2 cut(s) 246, 392
StyD4I CCNGG 2 cut(s) 233, 705
TaaI ACNGT 1 cut(s) 81
TaiI ACGT 2 cut(s) 32, 342
TaqI TCGA 2 cut(s) 93, 153
TasI AATT 6 cut(s) 9, 117, 329, 563, 857, 909
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 2 cut(s) 322, 814
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 2 cut(s) 63, 216
TseFI GTSAC 2 cut(s) 41, 631
TseI GCWGC 3 cut(s) 113, 415, 586
Tsp45I GTSAC 2 cut(s) 41, 631
TspDTI ATGAA 4 cut(s) 238, 587, 617, 827
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 2 cut(s) 63, 216
Van91I CCANNNNNTGG 1 cut(s) 712
VpaK11BI GGWCC 2 cut(s) 840, 917
XbaI TCTAGA 1 cut(s) 665
XceI RCATGY 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.