MD16G1011800.v1.1

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003272
Physical Location & Seq
Reverse (-)
905005 .. 906823
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1011800.v1.1.491

Sequence Viewer

Length: 957 bp
ATGACGCCAACGATAGTTCCTGAGGTAACACTGAGCTCTTGTGACATGACGATGCCAGTGATCGGCATGGGGACTTCATCATACCCTAAAGTTGACCCTGAAACAGCCAAGGCTGCAATTCTTGAAGCCATCAGAGCGGGCTACCGCCACTTCGACACGGCCTTCATTTACGGGTCAGAACAAGATCTCGGGGAAGCCATAGCTGAAGCACTCCGTCTTGGACTCGTCAAGTCCAGGAGTGAGCTCTTCATCACCACTAAGCTTTTTGCCAGCTTTGCTGAAAGAGACCTTGTAGTGCCTGCCATCAACATGAGCTTAAGGAATCTGCAATTGGAGTATGTGGATATGTACCTAATACATTGGCCCTTCAAATTTGGGAAAGTGGTGAGCTCTATGCCAGTGGAAAAGGGGGTGCTGCTACCCTTAGATATCAAGTCAGTTTGGGAAGGCATGGAAGAATGCAAGAGACTGGGCCTTGCGAGGGGCATTGGCGTGAGTAACTTCACTTCCGAGATGCTTGAGGACCTCCTTTCCATCGCCAAAATCCCTCCCGCCCTCAACCAATTGGAGGTGAACCCACTTTGGCAGCTGAAGAAACTGGGGGAGTTCTGTAAGGCAAAGGGAATTCATGTGACGGCCTACTCCCCGTTAGGTGCATCCGGGACTAGATTTGGAGAAAACAAAGTTTTAGGCACAAAAGTCCTCCAAGACATTGCCAAGTCCAAAGGAAAAACAACTGCTCAGATAGCCTTGAGATGGGTGTACCAGCAAGGGGTGAGCTTGATAACAAAGAGCTTCAATAAGGAAAGAATAAATCAGAACATTGACATCTTCGGTTGGTCCTTGACTGAGGAGGAATTAGACGAGATTAGTCGTCTTCCTCAACAGAAAACAATTATCTTTGCCTCAATTATGGGACCGCATGACGTTGTGCTCCAGATTGATGCTGGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

319

Amino Acids

35.21

Weight (kDa)

6.16

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 292 1.8e-53 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 137
AciI CCGC 4 cut(s) 137, 145, 552, 920
AcsI RAATTY 2 cut(s) 371, 624
AcuI CTGAAG 2 cut(s) 225, 611
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 350, 764
AfiI CCNNNNNNNGG 5 cut(s) 62, 481, 568, 756, 772
AflII CTTAAG 1 cut(s) 316
AgsI TTSAA 3 cut(s) 125, 370, 799
AjnI CCWGG 1 cut(s) 233
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
Alw21I GWGCWC 4 cut(s) 38, 246, 392, 936
Alw26I GTCTC 2 cut(s) 279, 460
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 4 cut(s) 159, 362, 472, 636
ApeKI GCWGC 3 cut(s) 113, 415, 586
ApoI RAATTY 2 cut(s) 371, 624
AspS9I GGNCC 5 cut(s) 363, 472, 523, 840, 917
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 4 cut(s) 244, 397, 583, 787
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 3 cut(s) 523, 840, 917
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 3 cut(s) 38, 246, 392
BarI GAAGNNNNNNTAC 2 cut(s) 490, 522
BbsI GAAGAC 1 cut(s) 869
Bbv12I GWGCWC 4 cut(s) 38, 246, 392, 936
BbvI GCAGC 3 cut(s) 100, 402, 598
BccI CCATC 4 cut(s) 137, 311, 542, 750
BceAI ACGGC 2 cut(s) 174, 651
BciT130I CCWGG 1 cut(s) 235
BcnI CCSGG 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 279, 460
BfaI CTAG 1 cut(s) 666
BfrI CTTAAG 1 cut(s) 316
BglII AGATCT 1 cut(s) 184
BisI GCNGC 3 cut(s) 114, 416, 587
BlsI GCNGC 3 cut(s) 115, 417, 588
Bme1390I CCNGG 2 cut(s) 235, 661
Bme18I GGWCC 3 cut(s) 523, 840, 917
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 5 cut(s) 363, 472, 523, 840, 917
BmiI GGNNCC 1 cut(s) 918
BmrFI CCNGG 2 cut(s) 235, 661
BmrI ACTGGG 2 cut(s) 479, 608
BmsI GCATC 4 cut(s) 42, 504, 665, 934
BmuI ACTGGG 2 cut(s) 479, 608
BpiI GAAGAC 1 cut(s) 869
BpmI CTGGAG 1 cut(s) 920
BpuEI CTTGAG 2 cut(s) 539, 772
BpuMI CCSGG 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 481, 568, 756, 772
Bse1I ACTGG 4 cut(s) 56, 398, 474, 603
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 711
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 656
BseLI CCNNNNNNNGG 5 cut(s) 62, 481, 568, 756, 772
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 4 cut(s) 12, 23, 755, 840
BseNI ACTGG 4 cut(s) 56, 398, 474, 603
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 100, 402, 598
BshFI GGCC 4 cut(s) 161, 364, 474, 638
BsiHKAI GWGCWC 4 cut(s) 38, 246, 392, 936
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 3 cut(s) 85, 676, 930
BslI CCNNNNNNNGG 5 cut(s) 62, 481, 568, 756, 772
BsmAI GTCTC 2 cut(s) 279, 460
BsmFI GGGAC 3 cut(s) 85, 676, 930
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 4 cut(s) 161, 364, 474, 638
Bso31I GGTCTC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 4 cut(s) 38, 246, 392, 936
Bsp143I GATC 2 cut(s) 60, 184
BspACI CCGC 4 cut(s) 137, 145, 552, 920
BspANI GGCC 4 cut(s) 161, 364, 474, 638
BspCNI CTCAG 4 cut(s) 13, 24, 754, 841
BspLI GGNNCC 1 cut(s) 918
BspQI GCTCTTC 1 cut(s) 251
BspTI CTTAAG 1 cut(s) 316
BspTNI GGTCTC 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 4 cut(s) 56, 398, 474, 603
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 2 cut(s) 60, 184
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 316
BstC8I GCNNGC 3 cut(s) 139, 271, 300
BstDEI CTNAG 6 cut(s) 21, 32, 258, 424, 741, 849
BstF5I GGATG 1 cut(s) 656
BstKTI GATC 2 cut(s) 63, 187
BstMAI GTCTC 2 cut(s) 279, 460
BstMBI GATC 2 cut(s) 60, 184
BstMWI GCNNNNNNNGC 4 cut(s) 113, 134, 275, 746
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 233, 659
BstV1I GCAGC 3 cut(s) 100, 402, 598
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 4 cut(s) 161, 364, 474, 638
BtgZI GCGATG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 656
BtsIMutI CAGTG 3 cut(s) 29, 63, 405
Cac8I GCNNGC 3 cut(s) 139, 271, 300
Cfr13I GGNCC 5 cut(s) 363, 472, 523, 840, 917
CseI GACGC 1 cut(s) 13
Csp6I GTAC 2 cut(s) 349, 763
CviAII CATG 6 cut(s) 46, 67, 310, 451, 629, 923
CviQI GTAC 2 cut(s) 349, 763
DdeI CTNAG 6 cut(s) 21, 32, 258, 424, 741, 849
DpnI GATC 2 cut(s) 62, 186
DpnII GATC 2 cut(s) 60, 184
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Ecl136II GAGCTC 3 cut(s) 36, 244, 390
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 3 cut(s) 38, 246, 392
Eco31I GGTCTC 1 cut(s) 279
Eco32I GATATC 1 cut(s) 430
Eco47I GGWCC 3 cut(s) 523, 840, 917
Eco53kI GAGCTC 3 cut(s) 36, 244, 390
Eco57I CTGAAG 2 cut(s) 225, 611
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 3 cut(s) 36, 244, 390
EcoO109I RGGNCCY 1 cut(s) 523
EcoRI GAATTC 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 233
EcoRV GATATC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 3 cut(s) 38, 246, 392
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 6 cut(s) 49, 70, 313, 454, 632, 926
FaqI GGGAC 3 cut(s) 85, 676, 930
FatI CATG 6 cut(s) 45, 66, 309, 450, 628, 922
FauI CCCGC 2 cut(s) 130, 559
Fnu4HI GCNGC 3 cut(s) 114, 416, 587
FokI GGATG 1 cut(s) 643
FriOI GRGCYC 3 cut(s) 38, 246, 392
Fsp4HI GCNGC 3 cut(s) 114, 416, 587
FspBI CTAG 1 cut(s) 666
GluI GCNGC 3 cut(s) 114, 416, 587
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 4 cut(s) 161, 364, 474, 638
HapII CCGG 1 cut(s) 660
HgaI GACGC 1 cut(s) 13
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 49, 70, 313, 454, 632, 926
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 2 cut(s) 222, 322
HpaII CCGG 1 cut(s) 660
HphI GGTGA 4 cut(s) 244, 397, 583, 787
Hpy166II GTNNAC 3 cut(s) 94, 574, 763
Hpy188I TCNGA 5 cut(s) 134, 178, 511, 744, 819
Hpy188III TCNNGA 3 cut(s) 20, 122, 937
Hpy8I GTNNAC 3 cut(s) 94, 574, 763
HpyAV CCTTC 3 cut(s) 172, 376, 440
HpyCH4IV ACGT 1 cut(s) 927
HpyCH4V TGCA 4 cut(s) 116, 328, 462, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 134, 275, 746
HpyF3I CTNAG 6 cut(s) 21, 32, 258, 424, 741, 849
HpySE526I ACGT 1 cut(s) 927
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 49, 70, 313, 454, 632, 926
Kzo9I GATC 2 cut(s) 60, 184
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 1 cut(s) 939
Lsp1109I GCAGC 3 cut(s) 100, 402, 598
LweI GCATC 4 cut(s) 42, 504, 665, 934
MaeI CTAG 1 cut(s) 666
MaeII ACGT 1 cut(s) 927
MaeIII GTNAC 4 cut(s) 25, 41, 497, 631
MalI GATC 2 cut(s) 62, 186
MbiI CCGCTC 1 cut(s) 137
MboI GATC 2 cut(s) 60, 184
MboII GAAGA 5 cut(s) 238, 467, 604, 823, 869
MfeI CAATTG 2 cut(s) 329, 563
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 4 cut(s) 38, 246, 392, 936
MluCI AATT 8 cut(s) 117, 329, 371, 563, 624, 857, 894, 909
MlyI GAGTC 1 cut(s) 216
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 3 cut(s) 50, 308, 491
MspA1I CMGCKG 1 cut(s) 589
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 2 cut(s) 235, 661
MunI CAATTG 2 cut(s) 329, 563
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 4 cut(s) 113, 134, 275, 746
NciI CCSGG 1 cut(s) 661
NdeII GATC 2 cut(s) 60, 184
NlaIII CATG 6 cut(s) 49, 70, 313, 454, 632, 926
NlaIV GGNNCC 1 cut(s) 918
NmuCI GTSAC 2 cut(s) 41, 631
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 322
PfoI TCCNGGA 2 cut(s) 233, 659
PkrI GCNGC 3 cut(s) 115, 417, 588
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PpuMI RGGWCCY 1 cut(s) 523
Psp124BI GAGCTC 3 cut(s) 38, 246, 392
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 918
PspPI GGNCC 5 cut(s) 363, 472, 523, 840, 917
PspPPI RGGWCCY 1 cut(s) 523
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 2 cut(s) 350, 764
RsaNI GTAC 2 cut(s) 349, 763
RseI CAYNNNNRTG 3 cut(s) 50, 308, 491
SacI GAGCTC 3 cut(s) 38, 246, 392
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 114, 416, 587
Sau3AI GATC 2 cut(s) 60, 184
Sau96I GGNCC 5 cut(s) 363, 472, 523, 840, 917
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 2 cut(s) 235, 661
SduI GDGCHC 4 cut(s) 38, 246, 392, 936
SfaNI GCATC 4 cut(s) 42, 504, 665, 934
SinI GGWCC 3 cut(s) 523, 840, 917
SmiMI CAYNNNNRTG 3 cut(s) 50, 308, 491
SmlI CTYRAG 3 cut(s) 316, 518, 751
SmoI CTYRAG 3 cut(s) 316, 518, 751
Sse9I AATT 8 cut(s) 117, 329, 371, 563, 624, 857, 894, 909
SsiI CCGC 4 cut(s) 137, 145, 552, 920
SspMI CTAG 1 cut(s) 666
SstI GAGCTC 3 cut(s) 38, 246, 392
StyD4I CCNGG 2 cut(s) 233, 659
StyI CCWWGG 1 cut(s) 108
TaiI ACGT 1 cut(s) 930
TaqI TCGA 1 cut(s) 153
TasI AATT 8 cut(s) 117, 329, 371, 563, 624, 857, 894, 909
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 3 cut(s) 36, 63, 405
TseFI GTSAC 2 cut(s) 41, 631
TseI GCWGC 3 cut(s) 113, 415, 586
Tsp45I GTSAC 2 cut(s) 41, 631
TspDTI ATGAA 4 cut(s) 66, 154, 238, 617
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 3 cut(s) 36, 63, 405
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 3 cut(s) 523, 840, 917
XapI RAATTY 2 cut(s) 371, 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.