Rh4CG449800

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003272
Physical Location & Seq
Forward (+)
68377572 .. 68378453
882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG449800.1

Sequence Viewer

Length: 447 bp
ATGGGAACTTCATCGCACCCTCCGGTGGACCGTCAAACGGCCAAGGCTGCCATTCTTGAAGCAATCAAAGTGGGTTACCTCCACTTTGACACCGCCTTTGCTTATGGTAATTCAGAGCAACATTTGGGGGAAGCTATTGCTGATGCTCTACGTCTTGGACTCATCGAGTCGAGGGATGAGCTCTTCATCACAACCAAGCTTTGGGCCAGCTTTGCTGAAAGAGATCTTGTGCTGCCTGCAATCAACACCAGTTTAAGGAATCTTCAAATGGAGTATGTTGATATGTACGTAATACATTGGCCATTAATTCAAATTGGGGCGAGAGATGACGGTAATTTCACCTCCAAGATGCTCGAGGAGCTCCCCTCCACCGCCAAAATCCCTCCTGCCATCAATCATGTGGAGATGAGCCCAGCTTGGCAGGTGAAGAAACTGAGGGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

148

Amino Acids

16.65

Weight (kDa)

5.48

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 6 - 101 7.3e-21 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 201
AciI CCGC 2 cut(s) 93, 372
AcoI YGGCCR 2 cut(s) 39, 299
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 4 cut(s) 25, 37, 201, 255
AgsI TTSAA 3 cut(s) 59, 266, 311
AluBI AGCT 6 cut(s) 134, 181, 199, 210, 361, 416
AluI AGCT 6 cut(s) 134, 181, 199, 210, 361, 416
Alw21I GWGCWC 2 cut(s) 183, 363
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 3 cut(s) 39, 204, 299
ApeKI GCWGC 2 cut(s) 47, 232
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AseI ATTAAT 1 cut(s) 305
AspS9I GGNCC 2 cut(s) 28, 204
AsuHPI GGTGA 2 cut(s) 331, 436
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 301
BanII GRGCYC 3 cut(s) 183, 363, 413
Bbv12I GWGCWC 2 cut(s) 183, 363
BbvI GCAGC 2 cut(s) 34, 219
BccI CCATC 1 cut(s) 398
BceAI ACGGC 1 cut(s) 54
BfuAI ACCTGC 1 cut(s) 412
BglII AGATCT 1 cut(s) 223
BisI GCNGC 2 cut(s) 48, 233
BlsI GCNGC 2 cut(s) 49, 234
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 2 cut(s) 28, 204
BmsI GCATC 2 cut(s) 133, 339
BplI GAGNNNNNCTC 2 cut(s) 350, 382
BsaAI YACGTR 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 42
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 37, 201, 255
Bse1I ACTGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 4 cut(s) 25, 37, 201, 255
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 2 cut(s) 34, 219
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 3 cut(s) 41, 206, 301
BsiHKAI GWGCWC 2 cut(s) 183, 363
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 1 cut(s) 23
BslI CCNNNNNNNGG 4 cut(s) 25, 37, 201, 255
BsnI GGCC 3 cut(s) 41, 206, 301
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 3 cut(s) 183, 363, 413
Bsp143I GATC 1 cut(s) 223
BspACI CCGC 2 cut(s) 93, 372
BspANI GGCC 3 cut(s) 41, 206, 301
BspCNI CTCAG 1 cut(s) 426
BspMI ACCTGC 1 cut(s) 412
BspQI GCTCTTC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 249
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 32, 332
Bst6I CTCTTC 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 2 cut(s) 208, 237
BstDEI CTNAG 1 cut(s) 434
BstEII GGTNACC 1 cut(s) 74
BstF5I GGATG 1 cut(s) 181
BstKTI GATC 1 cut(s) 226
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 3 cut(s) 47, 212, 358
BstPI GGTNACC 1 cut(s) 74
BstSNI TACGTA 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 34, 219
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BsuRI GGCC 3 cut(s) 41, 206, 301
BtsCI GGATG 1 cut(s) 181
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 208, 237
Cfr13I GGNCC 2 cut(s) 28, 204
Csp6I GTAC 1 cut(s) 286
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviAII CATG 1 cut(s) 398
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 1 cut(s) 434
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 39, 299
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Ecl136II GAGCTC 2 cut(s) 181, 361
Eco105I TACGTA 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 3 cut(s) 183, 363, 413
Eco47I GGWCC 1 cut(s) 28
Eco53kI GAGCTC 2 cut(s) 181, 361
Eco88I CYCGRG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 74
EcoICRI GAGCTC 2 cut(s) 181, 361
EcoO65I GGTNACC 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 42
EcoT38I GRGCYC 3 cut(s) 183, 363, 413
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 401
FaiI YATR 4 cut(s) 105, 276, 284, 399
FatI CATG 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 48, 233
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 3 cut(s) 183, 363, 413
Fsp4HI GCNGC 2 cut(s) 48, 233
GluI GCNGC 2 cut(s) 48, 233
GsaI CCCAGC 1 cut(s) 416
HaeIII GGCC 3 cut(s) 41, 206, 301
HapII CCGG 1 cut(s) 23
Hin1II CATG 1 cut(s) 401
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 3 cut(s) 159, 167, 259
HpaII CCGG 1 cut(s) 23
HphI GGTGA 2 cut(s) 331, 436
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 115
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 1 cut(s) 28
HpyCH4III ACNGT 2 cut(s) 32, 332
HpyCH4IV ACGT 2 cut(s) 151, 288
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 212, 358
HpyF3I CTNAG 1 cut(s) 434
HpySE526I ACGT 2 cut(s) 151, 288
Hsp92II CATG 1 cut(s) 401
Kzo9I GATC 1 cut(s) 223
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 2 cut(s) 358, 366
LpnPI CCDG 7 cut(s) 36, 220, 249, 262, 399, 407, 426
Lsp1109I GCAGC 2 cut(s) 34, 219
LweI GCATC 2 cut(s) 133, 339
MaeII ACGT 2 cut(s) 151, 288
MaeIII GTNAC 1 cut(s) 74
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MboII GAAGA 3 cut(s) 175, 254, 439
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 3 cut(s) 183, 363, 413
MlsI TGGCCA 1 cut(s) 301
MluCI AATT 4 cut(s) 109, 306, 312, 334
MluNI TGGCCA 1 cut(s) 301
MlyI GAGTC 2 cut(s) 153, 176
MnlI CCTC 8 cut(s) 30, 89, 165, 349, 352, 376, 393, 429
Mox20I TGGCCA 1 cut(s) 301
MscI TGGCCA 1 cut(s) 301
MseI TTAA 2 cut(s) 254, 305
Msp20I TGGCCA 1 cut(s) 301
MspI CCGG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 47, 212, 358
NdeII GATC 1 cut(s) 223
NlaIII CATG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 353
PaqCI CACCTGC 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 201
PkrI GCNGC 2 cut(s) 49, 234
PleI GAGTC 2 cut(s) 153, 175
PpsI GAGTC 2 cut(s) 153, 175
Ppu21I YACGTR 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 305
Psp124BI GAGCTC 2 cut(s) 183, 363
PspEI GGTNACC 1 cut(s) 74
PspFI CCCAGC 1 cut(s) 412
PspPI GGNCC 2 cut(s) 28, 204
PspXI VCTCGAGB 1 cut(s) 353
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SacI GAGCTC 2 cut(s) 183, 363
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 254, 305
SatI GCNGC 2 cut(s) 48, 233
Sau3AI GATC 1 cut(s) 223
Sau96I GGNCC 2 cut(s) 28, 204
SchI GAGTC 2 cut(s) 153, 176
SduI GDGCHC 3 cut(s) 183, 363, 413
SfaNI GCATC 2 cut(s) 133, 339
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 1 cut(s) 28
SlaI CTCGAG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SnaBI TACGTA 1 cut(s) 289
Sse9I AATT 4 cut(s) 109, 306, 312, 334
SsiI CCGC 2 cut(s) 93, 372
SstI GAGCTC 2 cut(s) 183, 363
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 32, 332
TaiI ACGT 2 cut(s) 154, 291
TaqI TCGA 3 cut(s) 165, 170, 354
TasI AATT 4 cut(s) 109, 306, 312, 334
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 254, 305
Tru9I TTAA 2 cut(s) 254, 305
TseI GCWGC 2 cut(s) 47, 232
TspDTI ATGAA 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XhoI CTCGAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.