Rh4CG449600

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003272
Physical Location & Seq
Forward (+)
68365799 .. 68367816
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG449600.1

Sequence Viewer

Length: 960 bp
ATGGCAAAGGTTCCTTCAGTTATCCTAAGCTCCTCCAGTGATGAGATCCTGACCATGCCAATCATCGGCATGGGAACTTCATCGTACCCTCGGGCCGACCCAGAAACCGCCAAGGCTGCCATTCTCGAAGCGATCAGAGCTGGTTACCGACATTTTGACACCGCCTTTGCTTACGGGTCGGAGCAAGATCTCGGTGAAGCCATAGCCGAGGCTCTCCGTATCGGACTCATCAACTCTAGGGATGAGCTCTTCATCACAACCAAGCTTTGGGCTAGTTTCGCCGAGAAAGACCTTGTGCTGCCGTCCATCAAAGCCAGTTTAAGGAATCTTCAACTAGAGTGCATTGACATGTACATCATACATTGGCCTTTCAAATTGGGAAAAGAGGTGAAAAGCATGCCTGTTGAGAGAGATCTGGTGCAGCCCCTTGATATCAAATCTGTTTGGGAAGCCATGGAAGAGTGCAAGAAACTTGGCCTTGCTAGAGGTATTGGCGTCAGTAACTTCACATGCAGTATGCTCGAGGAGCTTCTTTCCTTCGCCGAAATCCCTCCGGCCGTCAACCAATTGGAGATGAACCCAGCTTGGCAGCTGAAGAAATTGATGGACTTCTGCAAGGCAAAGGGTATTCATGTCACGGCTTACTCTCCGCTCGGAGCAGCTAGGACTAAATGGGGTGACAATAGGGTTTTGGGGTCAGATGTGATAGAAGAGATTGCCCTAGCCAGAGGAAAAACAACTGCTCAGATATCATTGAGATGGGTGTATGAGCAAGGTGTGAGCATAGTAACAAAAAGCTACAACAAGGAAAGAATGAGGCAGAACCTTGACATCTTCGACTTCTGCTTGACCGAGGAGGAATTGGAGAAGATGAGTCATCTTCCACAACGGAAAGGGGTTACCTTTGCCTCACTTCTAGGACCCCATGATATCGTTCTGGAAATTGACGCAGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

319

Amino Acids

35.64

Weight (kDa)

5.34

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 22 - 291 5.7e-53 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 652
AciI CCGC 3 cut(s) 108, 162, 650
AclWI GGATC 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 614
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 2 cut(s) 86, 353
AfiI CCNNNNNNNGG 3 cut(s) 65, 267, 321
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 2 cut(s) 332, 373
AluBI AGCT 9 cut(s) 30, 140, 247, 265, 529, 584, 592, 662, 798
AluI AGCT 9 cut(s) 30, 140, 247, 265, 529, 584, 592, 662, 798
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 1 cut(s) 40
Ama87I CYCGRG 2 cut(s) 90, 521
AoxI GGCC 4 cut(s) 93, 365, 475, 555
ApeKI GCWGC 5 cut(s) 116, 298, 421, 589, 659
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspS9I GGNCC 2 cut(s) 93, 920
AsuHPI GGTGA 3 cut(s) 206, 400, 689
AvaI CYCGRG 2 cut(s) 90, 521
AvaII GGWCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 5 cut(s) 103, 285, 433, 601, 671
BccI CCATC 3 cut(s) 314, 598, 753
BceAI ACGGC 3 cut(s) 286, 542, 654
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BfaI CTAG 7 cut(s) 237, 273, 335, 483, 663, 722, 917
BglII AGATCT 2 cut(s) 187, 412
BisI GCNGC 5 cut(s) 117, 299, 422, 590, 660
BlsI GCNGC 5 cut(s) 118, 300, 423, 591, 661
Bme18I GGWCC 1 cut(s) 920
BmeT110I CYCGRG 2 cut(s) 90, 521
BmgT120I GGNCC 2 cut(s) 93, 920
BmiI GGNNCC 2 cut(s) 12, 922
BpmI CTGGAG 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 5 cut(s) 89, 111, 207, 453, 852
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 267, 321
Bse1I ACTGG 2 cut(s) 36, 315
BseDI CCNNGG 5 cut(s) 89, 111, 207, 453, 852
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 3 cut(s) 65, 267, 321
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 2 cut(s) 36, 315
BseRI GAGGAG 3 cut(s) 22, 539, 869
BseX3I CGGCCG 1 cut(s) 555
BseXI GCAGC 5 cut(s) 103, 285, 433, 601, 671
BseYI CCCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 440
Bsh1285I CGRYCG 1 cut(s) 558
BshFI GGCC 4 cut(s) 95, 367, 477, 557
BsiEI CGRYCG 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 90, 521
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 3 cut(s) 65, 267, 321
BsnI GGCC 4 cut(s) 95, 367, 477, 557
BsoBI CYCGRG 2 cut(s) 90, 521
Bsp1286I GDGCHC 1 cut(s) 249
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 4 cut(s) 45, 132, 187, 412
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 3 cut(s) 108, 162, 650
BspANI GGCC 4 cut(s) 95, 367, 477, 557
BspCNI CTCAG 1 cut(s) 757
BspLI GGNNCC 2 cut(s) 12, 922
BspPI GGATC 1 cut(s) 40
BspQI GCTCTTC 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 652
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 2 cut(s) 36, 315
BssECI CCNNGG 5 cut(s) 89, 111, 207, 453, 852
BssMI GATC 4 cut(s) 45, 132, 187, 412
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 2 cut(s) 111, 453
Bst6I CTCTTC 3 cut(s) 254, 453, 705
BstACI GRCGYC 1 cut(s) 495
BstAUI TGTACA 1 cut(s) 351
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 2 cut(s) 26, 744
BstDSI CCRYGG 1 cut(s) 453
BstEII GGTNACC 2 cut(s) 143, 898
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 4 cut(s) 48, 135, 190, 415
BstMBI GATC 4 cut(s) 45, 132, 187, 412
BstMCI CGRYCG 1 cut(s) 558
BstMWI GCNNNNNNNGC 4 cut(s) 116, 137, 278, 526
BstNSI RCATGY 3 cut(s) 352, 400, 513
BstPI GGTNACC 2 cut(s) 143, 898
BstV1I GCAGC 5 cut(s) 103, 285, 433, 601, 671
BstX2I RGATCY 3 cut(s) 45, 187, 412
BstYI RGATCY 3 cut(s) 45, 187, 412
BstZI CGGCCG 1 cut(s) 555
BsuRI GGCC 4 cut(s) 95, 367, 477, 557
BtgI CCRYGG 1 cut(s) 453
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 93, 920
CseI GACGC 1 cut(s) 484
Csp6I GTAC 2 cut(s) 85, 352
CviAII CATG 8 cut(s) 55, 70, 349, 397, 454, 510, 632, 926
CviQI GTAC 2 cut(s) 85, 352
DdeI CTNAG 2 cut(s) 26, 744
DpnI GATC 4 cut(s) 47, 134, 189, 414
DpnII GATC 4 cut(s) 45, 132, 187, 412
EaeI YGGCCR 1 cut(s) 555
EagI CGGCCG 1 cut(s) 555
Eam1104I CTCTTC 3 cut(s) 254, 453, 705
EarI CTCTTC 3 cut(s) 254, 453, 705
Ecl136II GAGCTC 1 cut(s) 247
EclXI CGGCCG 1 cut(s) 555
Eco130I CCWWGG 2 cut(s) 111, 453
Eco24I GRGCYC 1 cut(s) 249
Eco32I GATATC 3 cut(s) 433, 750, 931
Eco47I GGWCC 1 cut(s) 920
Eco52I CGGCCG 1 cut(s) 555
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 614
Eco88I CYCGRG 2 cut(s) 90, 521
Eco91I GGTNACC 2 cut(s) 143, 898
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 920
EcoO65I GGTNACC 2 cut(s) 143, 898
EcoRV GATATC 3 cut(s) 433, 750, 931
EcoT14I CCWWGG 2 cut(s) 111, 453
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 2 cut(s) 111, 453
FaeI CATG 8 cut(s) 58, 73, 352, 400, 457, 513, 635, 929
FatI CATG 8 cut(s) 54, 69, 348, 396, 453, 509, 631, 925
Fnu4HI GCNGC 5 cut(s) 117, 299, 422, 590, 660
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 5 cut(s) 117, 299, 422, 590, 660
FspBI CTAG 7 cut(s) 237, 273, 335, 483, 663, 722, 917
GluI GCNGC 5 cut(s) 117, 299, 422, 590, 660
GsaI CCCAGC 1 cut(s) 584
GsuI CTGGAG 1 cut(s) 19
HaeIII GGCC 4 cut(s) 95, 367, 477, 557
HapII CCGG 1 cut(s) 554
HgaI GACGC 1 cut(s) 484
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 8 cut(s) 58, 73, 352, 400, 457, 513, 635, 929
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 3 cut(s) 225, 325, 874
HpaII CCGG 1 cut(s) 554
HphI GGTGA 3 cut(s) 206, 400, 689
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 6 cut(s) 137, 181, 224, 656, 700, 747
Hpy188III TCNNGA 3 cut(s) 49, 125, 938
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 2 cut(s) 24, 547
HpyCH4V TGCA 5 cut(s) 342, 421, 465, 513, 615
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 137, 278, 526
HpyF3I CTNAG 2 cut(s) 26, 744
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 8 cut(s) 58, 73, 352, 400, 457, 513, 635, 929
Kzo9I GATC 4 cut(s) 45, 132, 187, 412
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 4 cut(s) 35, 181, 526, 656
Lsp1109I GCAGC 5 cut(s) 103, 285, 433, 601, 671
MaeI CTAG 7 cut(s) 237, 273, 335, 483, 663, 722, 917
MaeIII GTNAC 6 cut(s) 143, 500, 634, 677, 787, 898
MalI GATC 4 cut(s) 47, 134, 189, 414
MbiI CCGCTC 1 cut(s) 652
MboI GATC 4 cut(s) 45, 132, 187, 412
MboII GAAGA 8 cut(s) 241, 320, 470, 607, 722, 826, 872, 880
MfeI CAATTG 1 cut(s) 566
MflI RGATCY 3 cut(s) 45, 187, 412
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 6 cut(s) 374, 566, 599, 860, 942, 953
MlyI GAGTC 2 cut(s) 219, 883
MmeI TCCRAC 1 cut(s) 159
MseI TTAA 1 cut(s) 320
MslI CAYNNNNRTG 3 cut(s) 68, 347, 757
MspA1I CMGCKG 1 cut(s) 592
MspI CCGG 1 cut(s) 554
MunI CAATTG 1 cut(s) 566
MwoI GCNNNNNNNGC 4 cut(s) 116, 137, 278, 526
NcoI CCATGG 1 cut(s) 453
NdeII GATC 4 cut(s) 45, 132, 187, 412
NlaIII CATG 8 cut(s) 58, 73, 352, 400, 457, 513, 635, 929
NlaIV GGNNCC 2 cut(s) 12, 922
NmeAIII GCCGAG 2 cut(s) 232, 307
NmuCI GTSAC 2 cut(s) 634, 677
NspI RCATGY 3 cut(s) 352, 400, 513
PaeI GCATGC 1 cut(s) 400
PaeR7I CTCGAG 1 cut(s) 521
PciI ACATGT 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 325
PflMI CCANNNNNTGG 1 cut(s) 267
PkrI GCNGC 5 cut(s) 118, 300, 423, 591, 661
PleI GAGTC 2 cut(s) 219, 882
PpsI GAGTC 2 cut(s) 219, 882
PpuMI RGGWCCY 1 cut(s) 920
PscI ACATGT 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 920
PspEI GGTNACC 2 cut(s) 143, 898
PspFI CCCAGC 1 cut(s) 580
PspN4I GGNNCC 2 cut(s) 12, 922
PspPI GGNCC 2 cut(s) 93, 920
PspPPI RGGWCCY 1 cut(s) 920
PspXI VCTCGAGB 1 cut(s) 521
PsuI RGATCY 3 cut(s) 45, 187, 412
PvuII CAGCTG 1 cut(s) 592
RsaI GTAC 2 cut(s) 86, 353
RsaNI GTAC 2 cut(s) 85, 352
RseI CAYNNNNRTG 3 cut(s) 68, 347, 757
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 5 cut(s) 117, 299, 422, 590, 660
Sau3AI GATC 4 cut(s) 45, 132, 187, 412
Sau96I GGNCC 2 cut(s) 93, 920
SchI GAGTC 2 cut(s) 219, 883
SduI GDGCHC 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 521
SinI GGWCC 1 cut(s) 920
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 3 cut(s) 68, 347, 757
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SphI GCATGC 1 cut(s) 400
Sse9I AATT 6 cut(s) 374, 566, 599, 860, 942, 953
SsiI CCGC 3 cut(s) 108, 162, 650
SspMI CTAG 7 cut(s) 237, 273, 335, 483, 663, 722, 917
SstI GAGCTC 1 cut(s) 249
StyI CCWWGG 2 cut(s) 111, 453
TaqI TCGA 3 cut(s) 126, 522, 837
TaqII GACCGA 1 cut(s) 866
TasI AATT 6 cut(s) 374, 566, 599, 860, 942, 953
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 2 cut(s) 634, 677
TseI GCWGC 5 cut(s) 116, 298, 421, 589, 659
Tsp45I GTSAC 2 cut(s) 634, 677
TspDTI ATGAA 4 cut(s) 69, 241, 590, 620
TspGWI ACGGA 2 cut(s) 206, 904
TspRI CASTG 1 cut(s) 43
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 920
XceI RCATGY 3 cut(s) 352, 400, 513
XhoI CTCGAG 1 cut(s) 521
XspI CTAG 7 cut(s) 237, 273, 335, 483, 663, 722, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.