MD16G1012000.v1.1

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003272
Physical Location & Seq
Reverse (-)
921448 .. 923147
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1012000.v1.1.491

Sequence Viewer

Length: 957 bp
ATGACGCCAATGATAGTTCCTGAGGTAACACTGAGCTCCTGTGACACGACGATGCCAGTGATCGGCATGGGGACTTCATCGTACCCTAAAGTTGACCCTGAAACAGCCAAGGCTGCAATTCTTGAAGCCATCAGAGCGGGCTACCGCCACTTCGACACTGCCTTTGTATACGGGTCAGAGCAAGATCTGGGGGAAGCCATAGCTGAGGCACTGCGTCTTGGACTCGTCAAGTCCAGGAGTGAGCTCTTCATCACCACCAAGCTTTTTGCCAGCTTTGCTGAAAGAGAGCTTGTAGTGCCTGCCATCAACAAGAGTTTAAGGAATCTGCAATTGGAGTATGTGGATATGTACCTAATACATTGGCCCTTCAAATTTGGGAAAGTGGTGAGCTCTATGCCAGTGGAAAAGGGGGTGCTGCTACCCTTAGACATCAAGTCAGTTTGGGAAGGCATGGAAGAATGCAAGAGATTGGGCCTTGCGAGGGGCATTGGCGTGAGTAACTTCACTTCCGAGATGATTGAGGACCTCCTTTCCATCGCCAAAATCCCTCCCGCCCTCAACCAATTGGAGATGAACCCACTTTGGCAGCTGAAGAAACTGAGGGAGTTCTGTAAGGCAAAGGGCATTCATGTAACGGCCTACTCTCCGTTAGGTTCATCCGGGACTAAATTTGGAGGCAACAAAGTCTTAGGCTCAAAAGTCCTCCAAGACATTGCCGAATCCAAAGGAAAAACAACTGCTCAGATAGCGTTGAGATGGGTGTACCAGCAAGGGGTGAGCTTGGTAACAAAGAGCGTCAACAAGGAAAGAATGAATCAGAACATTGACATCTTCGGTTGGTCCTTGACTGAGGAGGAATTAGACGAGATTAGTCGTCTTCCTCAACACAAAACAATCACCTTCGCCTCAATTATGGGACCGCATGATGTTGTGCTCCAGATCGATGCTGGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

319

Amino Acids

35.19

Weight (kDa)

6.55

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 292 4.3e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 137
AccI GTMKAC 1 cut(s) 168
AciI CCGC 4 cut(s) 137, 145, 552, 920
AcsI RAATTY 2 cut(s) 371, 668
AcuI CTGAAG 1 cut(s) 611
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 83, 350, 764
AfiI CCNNNNNNNGG 3 cut(s) 62, 481, 772
AgsI TTSAA 2 cut(s) 125, 370
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 1 cut(s) 233
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AluBI AGCT 9 cut(s) 36, 203, 244, 262, 273, 289, 390, 589, 780
AluI AGCT 9 cut(s) 36, 203, 244, 262, 273, 289, 390, 589, 780
Alw21I GWGCWC 4 cut(s) 38, 246, 392, 936
AoxI GGCC 3 cut(s) 362, 472, 636
ApeKI GCWGC 3 cut(s) 113, 415, 586
ApoI RAATTY 2 cut(s) 371, 668
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspS9I GGNCC 5 cut(s) 363, 472, 523, 840, 917
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 4 cut(s) 244, 397, 787, 889
AvaII GGWCC 3 cut(s) 523, 840, 917
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 3 cut(s) 38, 246, 392
BarI GAAGNNNNNNTAC 2 cut(s) 490, 522
BbsI GAAGAC 1 cut(s) 869
Bbv12I GWGCWC 4 cut(s) 38, 246, 392, 936
BbvCI CCTCAGC 1 cut(s) 204
BbvI GCAGC 3 cut(s) 100, 402, 598
BccI CCATC 4 cut(s) 137, 311, 542, 750
BceAI ACGGC 1 cut(s) 651
BciT130I CCWGG 1 cut(s) 235
BcnI CCSGG 1 cut(s) 661
BglII AGATCT 1 cut(s) 184
BisI GCNGC 3 cut(s) 114, 416, 587
BlsI GCNGC 3 cut(s) 115, 417, 588
Bme1390I CCNGG 2 cut(s) 235, 661
Bme18I GGWCC 3 cut(s) 523, 840, 917
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgT120I GGNCC 5 cut(s) 363, 472, 523, 840, 917
BmiI GGNNCC 1 cut(s) 918
BmrFI CCNGG 2 cut(s) 235, 661
BmsI GCATC 2 cut(s) 42, 934
BpiI GAAGAC 1 cut(s) 869
BpmI CTGGAG 1 cut(s) 920
Bpu10I CCTNAGC 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 661
Bsa29I ATCGAT 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 481, 772
Bse1I ACTGG 2 cut(s) 56, 398
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 711
BseBI CCWGG 1 cut(s) 235
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 656
BseLI CCNNNNNNNGG 3 cut(s) 62, 481, 772
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 6 cut(s) 12, 23, 195, 590, 755, 840
BseNI ACTGG 2 cut(s) 56, 398
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 100, 402, 598
BshFI GGCC 3 cut(s) 364, 474, 638
BshVI ATCGAT 1 cut(s) 942
BsiHKAI GWGCWC 4 cut(s) 38, 246, 392, 936
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 3 cut(s) 85, 676, 930
BslI CCNNNNNNNGG 3 cut(s) 62, 481, 772
BsmFI GGGAC 3 cut(s) 85, 676, 930
BsmI GAATGC 2 cut(s) 464, 624
BsnI GGCC 3 cut(s) 364, 474, 638
Bsp1286I GDGCHC 4 cut(s) 38, 246, 392, 936
Bsp143I GATC 3 cut(s) 60, 184, 939
BspACI CCGC 4 cut(s) 137, 145, 552, 920
BspANI GGCC 3 cut(s) 364, 474, 638
BspCNI CTCAG 6 cut(s) 13, 24, 196, 591, 754, 841
BspDI ATCGAT 1 cut(s) 942
BspLI GGNNCC 1 cut(s) 918
BspQI GCTCTTC 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 2 cut(s) 56, 398
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 3 cut(s) 60, 184, 939
BssNAI GTATAC 1 cut(s) 169
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 108
Bst1107I GTATAC 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 139, 271, 300
BstDEI CTNAG 8 cut(s) 21, 32, 204, 424, 599, 688, 741, 849
BstF5I GGATG 1 cut(s) 656
BstKTI GATC 3 cut(s) 63, 187, 942
BstMBI GATC 3 cut(s) 60, 184, 939
BstMWI GCNNNNNNNGC 5 cut(s) 113, 134, 275, 295, 746
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 233, 659
BstV1I GCAGC 3 cut(s) 100, 402, 598
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BstZ17I GTATAC 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 942
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 364, 474, 638
BsuTUI ATCGAT 1 cut(s) 942
BtgZI GCGATG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 656
BtsI GCAGTG 2 cut(s) 156, 209
BtsIMutI CAGTG 5 cut(s) 29, 63, 156, 209, 405
Cac8I GCNNGC 3 cut(s) 139, 271, 300
Cfr13I GGNCC 5 cut(s) 363, 472, 523, 840, 917
ClaI ATCGAT 1 cut(s) 942
CseI GACGC 3 cut(s) 13, 203, 784
Csp6I GTAC 3 cut(s) 82, 349, 763
CviAII CATG 4 cut(s) 67, 451, 629, 923
CviQI GTAC 3 cut(s) 82, 349, 763
DdeI CTNAG 8 cut(s) 21, 32, 204, 424, 599, 688, 741, 849
DpnI GATC 3 cut(s) 62, 186, 941
DpnII GATC 3 cut(s) 60, 184, 939
DriI GACNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 251
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 251
Ecl136II GAGCTC 3 cut(s) 36, 244, 390
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 3 cut(s) 38, 246, 392
Eco47I GGWCC 3 cut(s) 523, 840, 917
Eco53kI GAGCTC 3 cut(s) 36, 244, 390
Eco57I CTGAAG 1 cut(s) 611
Eco81I CCTNAGG 1 cut(s) 21
EcoICRI GAGCTC 3 cut(s) 36, 244, 390
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 233
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 3 cut(s) 38, 246, 392
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 4 cut(s) 70, 454, 632, 926
FaqI GGGAC 3 cut(s) 85, 676, 930
FatI CATG 4 cut(s) 66, 450, 628, 922
FauI CCCGC 2 cut(s) 130, 559
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 114, 416, 587
FokI GGATG 1 cut(s) 643
FriOI GRGCYC 3 cut(s) 38, 246, 392
Fsp4HI GCNGC 3 cut(s) 114, 416, 587
GluI GCNGC 3 cut(s) 114, 416, 587
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 3 cut(s) 364, 474, 638
HapII CCGG 1 cut(s) 660
HgaI GACGC 3 cut(s) 13, 203, 784
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 70, 454, 632, 926
HincII GTYRAC 2 cut(s) 94, 799
HindII GTYRAC 2 cut(s) 94, 799
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 4 cut(s) 222, 322, 719, 814
HpaII CCGG 1 cut(s) 660
HphI GGTGA 4 cut(s) 244, 397, 787, 889
Hpy166II GTNNAC 4 cut(s) 94, 169, 763, 799
Hpy188I TCNGA 5 cut(s) 134, 178, 511, 744, 819
Hpy188III TCNNGA 3 cut(s) 20, 122, 937
Hpy8I GTNNAC 4 cut(s) 94, 169, 763, 799
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 3 cut(s) 376, 440, 910
HpyCH4V TGCA 3 cut(s) 116, 328, 462
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 134, 275, 295, 746
HpyF3I CTNAG 8 cut(s) 21, 32, 204, 424, 599, 688, 741, 849
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 70, 454, 632, 926
Kzo9I GATC 3 cut(s) 60, 184, 939
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 2 cut(s) 41, 939
Lsp1109I GCAGC 3 cut(s) 100, 402, 598
LweI GCATC 2 cut(s) 42, 934
MaeIII GTNAC 5 cut(s) 25, 41, 497, 631, 784
MalI GATC 3 cut(s) 62, 186, 941
MbiI CCGCTC 1 cut(s) 137
MboI GATC 3 cut(s) 60, 184, 939
MboII GAAGA 5 cut(s) 238, 467, 604, 823, 869
MfeI CAATTG 2 cut(s) 329, 563
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 4 cut(s) 38, 246, 392, 936
MluCI AATT 7 cut(s) 117, 329, 371, 563, 668, 857, 909
MlyI GAGTC 1 cut(s) 216
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 2 cut(s) 50, 491
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 2 cut(s) 235, 661
MunI CAATTG 2 cut(s) 329, 563
Mva1269I GAATGC 2 cut(s) 464, 624
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 5 cut(s) 113, 134, 275, 295, 746
NciI CCSGG 1 cut(s) 661
NdeII GATC 3 cut(s) 60, 184, 939
NlaIII CATG 4 cut(s) 70, 454, 632, 926
NlaIV GGNNCC 1 cut(s) 918
NmuCI GTSAC 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 2 cut(s) 464, 624
PfeI GAWTC 3 cut(s) 322, 719, 814
PfoI TCCNGGA 2 cut(s) 233, 659
PkrI GCNGC 3 cut(s) 115, 417, 588
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PpuMI RGGWCCY 1 cut(s) 523
Psp124BI GAGCTC 3 cut(s) 38, 246, 392
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 918
PspPI GGNCC 5 cut(s) 363, 472, 523, 840, 917
PspPPI RGGWCCY 1 cut(s) 523
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 3 cut(s) 83, 350, 764
RsaNI GTAC 3 cut(s) 82, 349, 763
RseI CAYNNNNRTG 2 cut(s) 50, 491
SacI GAGCTC 3 cut(s) 38, 246, 392
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 114, 416, 587
Sau3AI GATC 3 cut(s) 60, 184, 939
Sau96I GGNCC 5 cut(s) 363, 472, 523, 840, 917
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 2 cut(s) 235, 661
SduI GDGCHC 4 cut(s) 38, 246, 392, 936
SfaNI GCATC 2 cut(s) 42, 934
SinI GGWCC 3 cut(s) 523, 840, 917
SmiMI CAYNNNNRTG 2 cut(s) 50, 491
Sse9I AATT 7 cut(s) 117, 329, 371, 563, 668, 857, 909
SsiI CCGC 4 cut(s) 137, 145, 552, 920
SstI GAGCTC 3 cut(s) 38, 246, 392
StyD4I CCNGG 2 cut(s) 233, 659
StyI CCWWGG 1 cut(s) 108
TaqI TCGA 2 cut(s) 153, 942
TasI AATT 7 cut(s) 117, 329, 371, 563, 668, 857, 909
TfiI GAWTC 3 cut(s) 322, 719, 814
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 5 cut(s) 36, 63, 163, 216, 405
TseFI GTSAC 1 cut(s) 41
TseI GCWGC 3 cut(s) 113, 415, 586
Tsp45I GTSAC 1 cut(s) 41
TspDTI ATGAA 6 cut(s) 66, 238, 587, 617, 645, 827
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 5 cut(s) 36, 63, 163, 216, 405
VpaK11BI GGWCC 3 cut(s) 523, 840, 917
XapI RAATTY 2 cut(s) 371, 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XmiI GTMKAC 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.