Rroxscaffold_5G00385940

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003272
Physical Location & Seq
Forward (+)
64930919 .. 64932901
1983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00385940.1

Sequence Viewer

Length: 906 bp
ATGCCTATCATCGGCATGGGAACTTCATCGTACCCTCGGGCCGACCCAGAAACCGCCAAGGCTGCCATTCTCGAAGCGATCAGAGCTGGTTACCGACATTTTGACACCGCCTTTGCCTACGGGTCGGAGCAAGATCTCGGTGAAGCCATAGCCGAGGCTCTCCGTCTCGGACTCATCAACTCTAGGGATGAGCTCTTCATCACAACCAAGCTTTGGGCTAGTTTCGCCGAGAAAGACCTTGTGGTGCCGTCCATCAAAGCCAGTTTAAGGAATCTTCAACTAGAGTACATTGACATGTACATCATACATTGGCCTTTCAAATTGGGAAAAGAGGTGAAAAGCATGCCTGTTGAGAGAGATCTGGTGCAGCCCCTTGATCTCAAATCTGTTTGGGAAGCCATGGTAGAGTGCAAGAAACTTGGCCTTGCTAGAGGTATTGGCGTTAGTAACTTCACATGCAGTATGCTCGAGGAGCTTCTTTCCTTCGCCGAAATCCCTCCGGCCGTCAACCAATTGGAGATGAACCCAGCTTGGCAGCTGAAGAAATTGAGGGACTTCTGCAAGGCAAAGGGTATTCATGTCACGGCTTACTCTCCGCTCGGAGCAGCTAGGACTAAATGGGGTGACAATAGGGTTTTGGGGTCAGATGTGATAGAAGAGATTGCCCTAGCCAGAGGAAAAACAACTGCTCAGATATCGTTGAGATGGGTGTATGAGCAAGGTGTGAGCATAGTAACAAAAAGCTACAACAAGGAAAGAATGAGGCAGAACCTTGACATCTTCGACTTCTGCTTGACCGAGGAGGAATTGGAGAAGATGAGTCATCTTCCACAACGGAAAGGGGTTACCTTTGCCTCACTTCTAGGACCCCATGATATCGTTCTGGAAATTGACGCAGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

301

Amino Acids

33.82

Weight (kDa)

5.69

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 4 - 273 1.4e-52 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AccB7I CCANNNNNTGG 1 cut(s) 213
AccBSI CCGCTC 1 cut(s) 598
AciI CCGC 3 cut(s) 54, 108, 596
AcoI YGGCCR 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 3 cut(s) 32, 287, 299
AfiI CCNNNNNNNGG 3 cut(s) 11, 213, 267
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 2 cut(s) 278, 319
AluBI AGCT 8 cut(s) 86, 193, 211, 475, 530, 538, 608, 744
AluI AGCT 8 cut(s) 86, 193, 211, 475, 530, 538, 608, 744
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 170
Ama87I CYCGRG 2 cut(s) 36, 467
AoxI GGCC 4 cut(s) 39, 311, 421, 501
ApeKI GCWGC 4 cut(s) 62, 367, 535, 605
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AspS9I GGNCC 2 cut(s) 39, 866
AsuHPI GGTGA 3 cut(s) 152, 346, 635
AvaI CYCGRG 2 cut(s) 36, 467
AvaII GGWCC 1 cut(s) 866
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 4 cut(s) 49, 379, 547, 617
BccI CCATC 2 cut(s) 260, 699
BceAI ACGGC 3 cut(s) 232, 488, 600
BcgI CGANNNNNNTGC 2 cut(s) 448, 482
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 7 cut(s) 183, 219, 281, 429, 609, 668, 863
BglII AGATCT 2 cut(s) 133, 358
BisI GCNGC 4 cut(s) 63, 368, 536, 606
BlsI GCNGC 4 cut(s) 64, 369, 537, 607
Bme18I GGWCC 1 cut(s) 866
BmeT110I CYCGRG 2 cut(s) 36, 467
BmgT120I GGNCC 2 cut(s) 39, 866
BmiI GGNNCC 2 cut(s) 246, 868
BsaJI CCNNGG 5 cut(s) 35, 57, 153, 399, 798
Bsc4I CCNNNNNNNGG 3 cut(s) 11, 213, 267
Bse1I ACTGG 1 cut(s) 261
BseDI CCNNGG 5 cut(s) 35, 57, 153, 399, 798
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 3 cut(s) 11, 213, 267
BseMII CTCAG 1 cut(s) 704
BseNI ACTGG 1 cut(s) 261
BseRI GAGGAG 2 cut(s) 485, 815
BseX3I CGGCCG 1 cut(s) 501
BseXI GCAGC 4 cut(s) 49, 379, 547, 617
BseYI CCCAGC 1 cut(s) 526
BsgI GTGCAG 1 cut(s) 386
Bsh1285I CGRYCG 1 cut(s) 504
BshFI GGCC 4 cut(s) 41, 313, 423, 503
BshNI GGYRCC 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 2 cut(s) 36, 467
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 1 cut(s) 566
BslI CCNNNNNNNGG 3 cut(s) 11, 213, 267
BsmAI GTCTC 1 cut(s) 170
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 566
BsnI GGCC 4 cut(s) 41, 313, 423, 503
BsoBI CYCGRG 2 cut(s) 36, 467
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 4 cut(s) 78, 133, 358, 376
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 3 cut(s) 54, 108, 596
BspANI GGCC 4 cut(s) 41, 313, 423, 503
BspCNI CTCAG 1 cut(s) 703
BspLI GGNNCC 2 cut(s) 246, 868
BspQI GCTCTTC 1 cut(s) 200
BspT107I GGYRCC 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 598
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 1 cut(s) 261
BssECI CCNNGG 5 cut(s) 35, 57, 153, 399, 798
BssMI GATC 4 cut(s) 78, 133, 358, 376
BssT1I CCWWGG 2 cut(s) 57, 399
Bst6I CTCTTC 2 cut(s) 200, 651
BstAUI TGTACA 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 344
BstDEI CTNAG 1 cut(s) 690
BstDSI CCRYGG 1 cut(s) 399
BstEII GGTNACC 2 cut(s) 89, 844
BstF5I GGATG 1 cut(s) 193
BstKTI GATC 4 cut(s) 81, 136, 361, 379
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 4 cut(s) 78, 133, 358, 376
BstMCI CGRYCG 1 cut(s) 504
BstMWI GCNNNNNNNGC 4 cut(s) 62, 83, 224, 472
BstNSI RCATGY 3 cut(s) 298, 346, 459
BstPI GGTNACC 2 cut(s) 89, 844
BstV1I GCAGC 4 cut(s) 49, 379, 547, 617
BstX2I RGATCY 2 cut(s) 133, 358
BstYI RGATCY 2 cut(s) 133, 358
BstZI CGGCCG 1 cut(s) 501
BsuRI GGCC 4 cut(s) 41, 313, 423, 503
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 344
Cfr13I GGNCC 2 cut(s) 39, 866
Csp6I GTAC 3 cut(s) 31, 286, 298
CviAII CATG 7 cut(s) 16, 295, 343, 400, 456, 578, 872
CviQI GTAC 3 cut(s) 31, 286, 298
DdeI CTNAG 1 cut(s) 690
DpnI GATC 4 cut(s) 80, 135, 360, 378
DpnII GATC 4 cut(s) 78, 133, 358, 376
EaeI YGGCCR 1 cut(s) 501
EagI CGGCCG 1 cut(s) 501
Eam1104I CTCTTC 2 cut(s) 200, 651
EarI CTCTTC 2 cut(s) 200, 651
Ecl136II GAGCTC 1 cut(s) 193
EclXI CGGCCG 1 cut(s) 501
Eco130I CCWWGG 2 cut(s) 57, 399
Eco24I GRGCYC 1 cut(s) 195
Eco32I GATATC 2 cut(s) 696, 877
Eco47I GGWCC 1 cut(s) 866
Eco52I CGGCCG 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 560
Eco88I CYCGRG 2 cut(s) 36, 467
Eco91I GGTNACC 2 cut(s) 89, 844
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 866
EcoO65I GGTNACC 2 cut(s) 89, 844
EcoRV GATATC 2 cut(s) 696, 877
EcoT14I CCWWGG 2 cut(s) 57, 399
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 2 cut(s) 57, 399
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 7 cut(s) 19, 298, 346, 403, 459, 581, 875
FaqI GGGAC 1 cut(s) 566
FatI CATG 7 cut(s) 15, 294, 342, 399, 455, 577, 871
Fnu4HI GCNGC 4 cut(s) 63, 368, 536, 606
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 4 cut(s) 63, 368, 536, 606
FspBI CTAG 7 cut(s) 183, 219, 281, 429, 609, 668, 863
GluI GCNGC 4 cut(s) 63, 368, 536, 606
GsaI CCCAGC 1 cut(s) 530
HaeIII GGCC 4 cut(s) 41, 313, 423, 503
HapII CCGG 1 cut(s) 500
Hin1II CATG 7 cut(s) 19, 298, 346, 403, 459, 581, 875
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 3 cut(s) 171, 271, 820
HpaII CCGG 1 cut(s) 500
HphI GGTGA 3 cut(s) 152, 346, 635
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 6 cut(s) 83, 127, 170, 602, 646, 693
Hpy188III TCNNGA 2 cut(s) 71, 884
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 1 cut(s) 493
HpyCH4V TGCA 4 cut(s) 367, 411, 459, 561
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 83, 224, 472
HpyF3I CTNAG 1 cut(s) 690
Hsp92II CATG 7 cut(s) 19, 298, 346, 403, 459, 581, 875
Kzo9I GATC 4 cut(s) 78, 133, 358, 376
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 3 cut(s) 127, 472, 602
LpnPI CCDG 9 cut(s) 60, 72, 274, 347, 360, 513, 540, 685, 869
Lsp1109I GCAGC 4 cut(s) 49, 379, 547, 617
MaeI CTAG 7 cut(s) 183, 219, 281, 429, 609, 668, 863
MaeIII GTNAC 6 cut(s) 89, 446, 580, 623, 733, 844
MalI GATC 4 cut(s) 80, 135, 360, 378
MbiI CCGCTC 1 cut(s) 598
MboI GATC 4 cut(s) 78, 133, 358, 376
MboII GAAGA 7 cut(s) 187, 266, 553, 668, 772, 818, 826
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 2 cut(s) 133, 358
MhlI GDGCHC 1 cut(s) 195
MluCI AATT 6 cut(s) 320, 512, 545, 806, 888, 899
MlyI GAGTC 2 cut(s) 165, 829
MmeI TCCRAC 1 cut(s) 105
MseI TTAA 1 cut(s) 266
MslI CAYNNNNRTG 2 cut(s) 14, 293
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 1 cut(s) 500
MunI CAATTG 1 cut(s) 512
MwoI GCNNNNNNNGC 4 cut(s) 62, 83, 224, 472
NcoI CCATGG 1 cut(s) 399
NdeII GATC 4 cut(s) 78, 133, 358, 376
NlaIII CATG 7 cut(s) 19, 298, 346, 403, 459, 581, 875
NlaIV GGNNCC 2 cut(s) 246, 868
NmeAIII GCCGAG 2 cut(s) 178, 253
NmuCI GTSAC 2 cut(s) 580, 623
NspI RCATGY 3 cut(s) 298, 346, 459
PaeI GCATGC 1 cut(s) 346
PaeR7I CTCGAG 1 cut(s) 467
PciI ACATGT 1 cut(s) 294
PciSI GCTCTTC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 213
PkrI GCNGC 4 cut(s) 64, 369, 537, 607
PleI GAGTC 2 cut(s) 165, 828
PpsI GAGTC 2 cut(s) 165, 828
PpuMI RGGWCCY 1 cut(s) 866
PscI ACATGT 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 866
PspEI GGTNACC 2 cut(s) 89, 844
PspFI CCCAGC 1 cut(s) 526
PspN4I GGNNCC 2 cut(s) 246, 868
PspPI GGNCC 2 cut(s) 39, 866
PspPPI RGGWCCY 1 cut(s) 866
PspXI VCTCGAGB 1 cut(s) 467
PsuI RGATCY 2 cut(s) 133, 358
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 3 cut(s) 32, 287, 299
RsaNI GTAC 3 cut(s) 31, 286, 298
RseI CAYNNNNRTG 2 cut(s) 14, 293
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 4 cut(s) 63, 368, 536, 606
Sau3AI GATC 4 cut(s) 78, 133, 358, 376
Sau96I GGNCC 2 cut(s) 39, 866
SchI GAGTC 2 cut(s) 165, 829
SduI GDGCHC 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 467
SinI GGWCC 1 cut(s) 866
SlaI CTCGAG 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 14, 293
SmlI CTYRAG 1 cut(s) 467
SmoI CTYRAG 1 cut(s) 467
SphI GCATGC 1 cut(s) 346
Sse9I AATT 6 cut(s) 320, 512, 545, 806, 888, 899
SsiI CCGC 3 cut(s) 54, 108, 596
SspMI CTAG 7 cut(s) 183, 219, 281, 429, 609, 668, 863
SstI GAGCTC 1 cut(s) 195
StyI CCWWGG 2 cut(s) 57, 399
TaqI TCGA 3 cut(s) 72, 468, 783
TaqII GACCGA 1 cut(s) 812
TasI AATT 6 cut(s) 320, 512, 545, 806, 888, 899
TatI WGTACW 2 cut(s) 285, 297
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TseFI GTSAC 2 cut(s) 580, 623
TseI GCWGC 4 cut(s) 62, 367, 535, 605
Tsp45I GTSAC 2 cut(s) 580, 623
TspDTI ATGAA 4 cut(s) 15, 187, 536, 566
TspGWI ACGGA 2 cut(s) 152, 850
Van91I CCANNNNNTGG 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 866
XceI RCATGY 3 cut(s) 298, 346, 459
XhoI CTCGAG 1 cut(s) 467
XspI CTAG 7 cut(s) 183, 219, 281, 429, 609, 668, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.