FvH4_5g03020

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001923
Physical Location & Seq
Reverse (-)
1810238 .. 1812517
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03020.t1

Sequence Viewer

Length: 879 bp
ATGGCGACCGGAGTTTCGGTTGCTTCGGTCCCAGTATGGTACTGTTACGGTCGAAACAATCTACTTGCCAGAAAACCCATCACCCATACTAGAAGCCCCGGTTTTGGTTTGGTTTCAACAAAGAAGAGACGCGTGGTTATGGAAAAGAGAACAGAGCATGAATATACAGAGAAAGCCTTGGAACACACCGAGAGATCAAGTGTTGTAACTCCAAGAGTTTCAGAGAGATTAGCACGAAAGAGATCAGAGAGAAAAACTTACCTTGTTGCTGCAATCATGTCCAGCCTGGGAGTCACTTCAGCTGCTGCCTTGGCTGTTTACTACAGATTTGCTTGGCAAACAGAGACTGGGGAGTTTCTGGTTCCAGAGATGATTGGCACATTTCTTCTCACTATGGGATCTGCTGTGGGGATGGAGTTTTGGGCAAGGTGGGCACATAGAGCTCTGTGGCATGCACAATTGTGGCAAATGCATGAGTCTCACCATCGACCAAGGGATGGTGCTTTCGAGCTCAACGATGTGTTTGCTATAACCAATGCTGCTCCTGCTATTGCTCTCCTCTCTTATGGTTTCTTCAACAACGGTCTTTTTCCCGGACTATGTTTTGGTGCTGGGTTAGGGATCACAATTTTTGGGATAGCATACATGTTTGTGCACGATGGCCTCGTTCACCGTCGATTTCCGGTCGGACCAATCGCCGACGTCCCTTATTTACGAAAAGTTGCTGCCGCCCACCAGCTTCATCATACATACATATTCAATGGAGTGCCATATGGGTTGTTCTTGGGACCCAAGGAAATAGAAGAGGTTGGAGGCATGGAAGAATTGGAAACGGAGATCAAGCGGAGAAGCACCAAGATTACTATAGCCAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

293

Amino Acids

32.78

Weight (kDa)

9.58

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 135 - 261 8e-14 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 705
AccB7I CCANNNNNTGG 1 cut(s) 497
AccII CGCG 1 cut(s) 132
AciI CCGC 2 cut(s) 729, 844
AclWI GGATC 2 cut(s) 406, 629
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 104, 497
AflIII ACRYGT 2 cut(s) 130, 645
AgsI TTSAA 3 cut(s) 117, 577, 760
AjnI CCWGG 1 cut(s) 285
AleI CACNNNNGTG 1 cut(s) 460
AluBI AGCT 4 cut(s) 302, 443, 511, 739
AluI AGCT 4 cut(s) 302, 443, 511, 739
Alw21I GWGCWC 3 cut(s) 445, 513, 657
Alw26I GTCTC 3 cut(s) 121, 338, 483
Alw44I GTGCAC 1 cut(s) 653
AlwI GGATC 2 cut(s) 406, 629
AlwNI CAGNNNCTG 2 cut(s) 305, 347
AoxI GGCC 1 cut(s) 661
ApaLI GTGCAC 1 cut(s) 653
ApeKI GCWGC 5 cut(s) 269, 302, 305, 539, 725
ArsI GACNNNNNNTTYG 1 cut(s) 30
AspS9I GGNCC 3 cut(s) 28, 689, 788
AsuC2I CCSGG 2 cut(s) 99, 594
AsuHPI GGTGA 3 cut(s) 73, 473, 662
AvaII GGWCC 3 cut(s) 28, 689, 788
BaeGI GKGCMC 2 cut(s) 436, 657
BanII GRGCYC 2 cut(s) 445, 513
Bbv12I GWGCWC 3 cut(s) 445, 513, 657
BbvI GCAGC 5 cut(s) 256, 289, 292, 526, 712
BccI CCATC 5 cut(s) 86, 406, 491, 492, 653
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 1 cut(s) 287
BcnI CCSGG 2 cut(s) 99, 594
BcoDI GTCTC 3 cut(s) 121, 338, 483
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 2 cut(s) 322, 864
BisI GCNGC 6 cut(s) 270, 303, 306, 540, 726, 729
BlsI GCNGC 6 cut(s) 271, 304, 307, 541, 727, 730
Bme1390I CCNGG 3 cut(s) 99, 287, 594
Bme18I GGWCC 3 cut(s) 28, 689, 788
BmgT120I GGNCC 3 cut(s) 28, 689, 788
BmiI GGNNCC 4 cut(s) 30, 363, 789, 790
BmrFI CCNGG 3 cut(s) 99, 287, 594
BmrI ACTGGG 2 cut(s) 26, 357
BmuI ACTGGG 2 cut(s) 26, 357
BpuMI CCSGG 2 cut(s) 99, 594
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 6 cut(s) 97, 177, 286, 309, 491, 792
BsaWI WCCGGW 2 cut(s) 8, 682
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 497
Bse1I ACTGG 2 cut(s) 32, 352
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 6 cut(s) 97, 177, 286, 309, 491, 792
BseGI GGATG 2 cut(s) 417, 502
BseLI CCNNNNNNNGG 2 cut(s) 104, 497
BseNI ACTGG 2 cut(s) 32, 352
BseRI GAGGAG 1 cut(s) 548
BseSI GKGCMC 2 cut(s) 436, 657
BseXI GCAGC 5 cut(s) 256, 289, 292, 526, 712
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 3 cut(s) 9, 52, 687
BshFI GGCC 1 cut(s) 663
BsiEI CGRYCG 3 cut(s) 9, 52, 687
BsiHKAI GWGCWC 3 cut(s) 445, 513, 657
BsiSI CCGG 4 cut(s) 9, 99, 594, 683
BslFI GGGAC 3 cut(s) 14, 689, 801
BslI CCNNNNNNNGG 2 cut(s) 104, 497
BsmAI GTCTC 3 cut(s) 121, 338, 483
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 3 cut(s) 14, 689, 801
BsnI GGCC 1 cut(s) 663
Bsp1286I GDGCHC 4 cut(s) 436, 445, 513, 657
Bsp143I GATC 5 cut(s) 194, 242, 398, 621, 837
BspACI CCGC 2 cut(s) 729, 844
BspANI GGCC 1 cut(s) 663
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 4 cut(s) 30, 363, 789, 790
BspPI GGATC 2 cut(s) 406, 629
BsrI ACTGG 2 cut(s) 32, 352
BssECI CCNNGG 6 cut(s) 97, 177, 286, 309, 491, 792
BssMI GATC 5 cut(s) 194, 242, 398, 621, 837
BssNI GRCGYC 1 cut(s) 702
BssT1I CCWWGG 4 cut(s) 177, 309, 491, 792
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 4 cut(s) 44, 50, 584, 674
Bst6I CTCTTC 2 cut(s) 119, 798
BstACI GRCGYC 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 453
BstF5I GGATG 2 cut(s) 417, 502
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 5 cut(s) 197, 245, 401, 624, 840
BstMAI GTCTC 3 cut(s) 121, 338, 483
BstMBI GATC 5 cut(s) 194, 242, 398, 621, 837
BstMCI CGRYCG 3 cut(s) 9, 52, 687
BstMWI GCNNNNNNNGC 4 cut(s) 311, 431, 440, 545
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 2 cut(s) 455, 649
BstSCI CCNGG 3 cut(s) 97, 285, 592
BstSFI CTRYAG 2 cut(s) 322, 864
BstSLI GKGCMC 2 cut(s) 436, 657
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 5 cut(s) 256, 289, 292, 526, 712
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BsuRI GGCC 1 cut(s) 663
BtsCI GGATG 2 cut(s) 417, 502
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 2 cut(s) 305, 347
Cfr13I GGNCC 3 cut(s) 28, 689, 788
CseI GACGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 40
CviAII CATG 6 cut(s) 158, 277, 452, 473, 646, 817
CviQI GTAC 1 cut(s) 40
DpnI GATC 5 cut(s) 196, 244, 400, 623, 839
DpnII GATC 5 cut(s) 194, 242, 398, 621, 837
Eam1104I CTCTTC 2 cut(s) 119, 798
EarI CTCTTC 2 cut(s) 119, 798
Ecl136II GAGCTC 2 cut(s) 443, 511
Eco130I CCWWGG 4 cut(s) 177, 309, 491, 792
Eco24I GRGCYC 2 cut(s) 445, 513
Eco47I GGWCC 3 cut(s) 28, 689, 788
Eco53kI GAGCTC 2 cut(s) 443, 511
Eco57I CTGAAG 1 cut(s) 282
EcoICRI GAGCTC 2 cut(s) 443, 511
EcoO109I RGGNCCY 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 4 cut(s) 177, 309, 491, 792
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 445, 513
ErhI CCWWGG 4 cut(s) 177, 309, 491, 792
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 6 cut(s) 161, 280, 455, 476, 649, 820
FaqI GGGAC 3 cut(s) 14, 689, 801
FatI CATG 6 cut(s) 157, 276, 451, 472, 645, 816
FauNDI CATATG 1 cut(s) 772
Fnu4HI GCNGC 6 cut(s) 270, 303, 306, 540, 726, 729
FokI GGATG 2 cut(s) 424, 509
FriOI GRGCYC 2 cut(s) 445, 513
Fsp4HI GCNGC 6 cut(s) 270, 303, 306, 540, 726, 729
FspBI CTAG 1 cut(s) 90
GluI GCNGC 6 cut(s) 270, 303, 306, 540, 726, 729
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 1 cut(s) 663
HapII CCGG 4 cut(s) 9, 99, 594, 683
HgaI GACGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 6 cut(s) 161, 280, 455, 476, 649, 820
HinfI GANTC 2 cut(s) 291, 476
HpaII CCGG 4 cut(s) 9, 99, 594, 683
HphI GGTGA 3 cut(s) 73, 473, 662
Hpy166II GTNNAC 3 cut(s) 319, 655, 670
Hpy188I TCNGA 3 cut(s) 223, 247, 689
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 3 cut(s) 319, 655, 670
Hpy99I CGWCG 2 cut(s) 678, 704
HpyCH4III ACNGT 4 cut(s) 44, 50, 584, 674
HpyCH4IV ACGT 1 cut(s) 702
HpyCH4V TGCA 4 cut(s) 272, 455, 472, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 311, 431, 440, 545
HpySE526I ACGT 1 cut(s) 702
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 6 cut(s) 161, 280, 455, 476, 649, 820
KflI GGGWCCC 1 cut(s) 788
Kzo9I GATC 5 cut(s) 194, 242, 398, 621, 837
LmnI GCTCC 1 cut(s) 547
Lsp1109I GCAGC 5 cut(s) 256, 289, 292, 526, 712
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 3 cut(s) 44, 205, 292
MalI GATC 5 cut(s) 196, 244, 400, 623, 839
MboI GATC 5 cut(s) 194, 242, 398, 621, 837
MboII GAAGA 5 cut(s) 136, 377, 565, 815, 833
MfeI CAATTG 1 cut(s) 458
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 4 cut(s) 436, 445, 513, 657
MluCI AATT 3 cut(s) 458, 627, 824
MluI ACGCGT 1 cut(s) 130
MlyI GAGTC 2 cut(s) 300, 485
MmeI TCCRAC 2 cut(s) 667, 790
MnlI CCTC 4 cut(s) 569, 674, 799, 806
Mph1103I ATGCAT 1 cut(s) 474
MslI CAYNNNNRTG 2 cut(s) 460, 650
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 4 cut(s) 9, 99, 594, 683
MspR9I CCNGG 3 cut(s) 99, 287, 594
MunI CAATTG 1 cut(s) 458
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 4 cut(s) 311, 431, 440, 545
NciI CCSGG 2 cut(s) 99, 594
NdeI CATATG 1 cut(s) 772
NdeII GATC 5 cut(s) 194, 242, 398, 621, 837
NlaIII CATG 6 cut(s) 161, 280, 455, 476, 649, 820
NlaIV GGNNCC 4 cut(s) 30, 363, 789, 790
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 2 cut(s) 455, 649
OliI CACNNNNGTG 1 cut(s) 460
PaeI GCATGC 1 cut(s) 455
PciI ACATGT 1 cut(s) 645
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 663
PflMI CCANNNNNTGG 1 cut(s) 497
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 6 cut(s) 271, 304, 307, 541, 727, 730
PleI GAGTC 2 cut(s) 299, 484
PpsI GAGTC 2 cut(s) 299, 484
PpuMI RGGWCCY 1 cut(s) 788
PscI ACATGT 1 cut(s) 645
Psp124BI GAGCTC 2 cut(s) 445, 513
Psp5II RGGWCCY 1 cut(s) 788
Psp6I CCWGG 1 cut(s) 285
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 4 cut(s) 30, 363, 789, 790
PspPI GGNCC 3 cut(s) 28, 689, 788
PspPPI RGGWCCY 1 cut(s) 788
PstNI CAGNNNCTG 2 cut(s) 305, 347
PsuI RGATCY 1 cut(s) 398
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 2 cut(s) 460, 650
SacI GAGCTC 2 cut(s) 445, 513
SatI GCNGC 6 cut(s) 270, 303, 306, 540, 726, 729
Sau3AI GATC 5 cut(s) 194, 242, 398, 621, 837
Sau96I GGNCC 3 cut(s) 28, 689, 788
SchI GAGTC 2 cut(s) 300, 485
ScrFI CCNGG 3 cut(s) 99, 287, 594
SduI GDGCHC 4 cut(s) 436, 445, 513, 657
SetI ASST 8 cut(s) 264, 304, 431, 445, 513, 705, 741, 810
SfcI CTRYAG 2 cut(s) 322, 864
SinI GGWCC 3 cut(s) 28, 689, 788
SmiMI CAYNNNNRTG 2 cut(s) 460, 650
SphI GCATGC 1 cut(s) 455
Sse9I AATT 3 cut(s) 458, 627, 824
SsiI CCGC 2 cut(s) 729, 844
SspMI CTAG 1 cut(s) 90
SstI GAGCTC 2 cut(s) 445, 513
StyD4I CCNGG 3 cut(s) 97, 285, 592
StyI CCWWGG 4 cut(s) 177, 309, 491, 792
TaaI ACNGT 4 cut(s) 44, 50, 584, 674
TaiI ACGT 1 cut(s) 705
TaqI TCGA 4 cut(s) 52, 487, 507, 676
TaqII GACCGA 1 cut(s) 16
TasI AATT 3 cut(s) 458, 627, 824
TauI GCSGC 1 cut(s) 731
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 5 cut(s) 269, 302, 305, 539, 725
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 174, 731
TspGWI ACGGA 1 cut(s) 848
Van91I CCANNNNNTGG 1 cut(s) 497
VneI GTGCAC 1 cut(s) 653
VpaK11BI GGWCC 3 cut(s) 28, 689, 788
XceI RCATGY 2 cut(s) 455, 649
XspI CTAG 1 cut(s) 90
ZraI GACGTC 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.