Rroxscaffold_3G00258920

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001923
Physical Location & Seq
Reverse (-)
53330194 .. 53331899
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00258920.1

Sequence Viewer

Length: 603 bp
ATGTCCAGCCTGGGAGTCACTTCAGCTGCTGCCATGGCTGTTTACTACAGATTTTCTTGGCAAACAGAGATTGGGGAGTTTCTTGTTCCAGAGATGTTGGGCACATTTTTGCTCTCTGTGGGCTCTGCTGTGGGGATGGAGTTTTGGGCAAGGTGGGCGCACAAAGCTCTGTGGCATGCTTCATTGTGGCAAATGCATGAGTCTCACCATCGACCTAGGGATGGTCCTTTCGAGATTAACGACGTGTTTGCTATAATCAATGCTGCTCCTGCCATTGCTCTTCTCTATTACGGTTTCTTTACCAAGGGTCTTTTTCCTGGACTATGTTTTGGTGCTGGGCTAGGGATCACAGTTTTCGGGATGGCATACATGTTTGTGCACGATGGTCTCGTCCACCGTCGATTTCCAGTCGGGCCAATCGCAGACGTCCCTTATTTACAAAGAGTTGCTGCCGCCCACCAGATTCATCATACATACATATTGAATGGAGTGCCGTATGGGTTGTTCTTGGGACCTAAGGAAATAGAAGAGGTTGGAGGCATGGAAGAATTAGAAAAGGAGATCAAGCGGAGAAGCACCAAGAGTACTATACCCAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

200

Amino Acids

22.32

Weight (kDa)

6.79

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 43 - 169 9.1e-15 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
AciI CCGC 2 cut(s) 453, 568
AclWI GGATC 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 1 cut(s) 221
AflIII ACRYGT 2 cut(s) 243, 369
AgsI TTSAA 1 cut(s) 484
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 2 cut(s) 9, 316
AluBI AGCT 3 cut(s) 26, 167, 600
AluI AGCT 3 cut(s) 26, 167, 600
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 2 cut(s) 207, 392
Alw44I GTGCAC 1 cut(s) 377
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 29
AoxI GGCC 1 cut(s) 413
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 4 cut(s) 26, 29, 263, 449
AspA2I CCTAGG 1 cut(s) 215
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 3 cut(s) 224, 413, 512
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 2 cut(s) 224, 512
AvrII CCTAGG 1 cut(s) 215
AxyI CCTNAGG 1 cut(s) 516
BaeGI GKGCMC 2 cut(s) 104, 381
BanII GRGCYC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 4 cut(s) 13, 16, 250, 436
BccI CCATC 5 cut(s) 130, 215, 216, 355, 377
BceAI ACGGC 1 cut(s) 478
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 2 cut(s) 11, 318
BcoDI GTCTC 2 cut(s) 207, 392
BfaI CTAG 2 cut(s) 216, 341
BfmI CTRYAG 1 cut(s) 46
BisI GCNGC 5 cut(s) 27, 30, 264, 450, 453
BlnI CCTAGG 1 cut(s) 215
BlsI GCNGC 5 cut(s) 28, 31, 265, 451, 454
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 2 cut(s) 11, 318
Bme18I GGWCC 2 cut(s) 224, 512
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 3 cut(s) 224, 413, 512
BmiI GGNNCC 1 cut(s) 513
BmrFI CCNGG 2 cut(s) 11, 318
BsaHI GRCGYC 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 4 cut(s) 10, 33, 215, 303
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 407
Bse21I CCTNAGG 1 cut(s) 516
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 2 cut(s) 11, 318
BseDI CCNNGG 4 cut(s) 10, 33, 215, 303
BseGI GGATG 3 cut(s) 141, 226, 366
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMI GCAATG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 407
BseSI GKGCMC 2 cut(s) 104, 381
BseXI GCAGC 4 cut(s) 13, 16, 250, 436
BseYI CCCAGC 1 cut(s) 335
BshFI GGCC 1 cut(s) 415
BsiHKAI GWGCWC 1 cut(s) 381
BslFI GGGAC 2 cut(s) 413, 525
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 2 cut(s) 207, 392
BsmFI GGGAC 2 cut(s) 413, 525
BsnI GGCC 1 cut(s) 415
Bso31I GGTCTC 1 cut(s) 392
Bsp1286I GDGCHC 3 cut(s) 104, 125, 381
Bsp143I GATC 2 cut(s) 345, 561
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 2 cut(s) 453, 568
BspANI GGCC 1 cut(s) 415
BspLI GGNNCC 1 cut(s) 513
BspPI GGATC 1 cut(s) 353
BspQI GCTCTTC 1 cut(s) 285
BspTNI GGTCTC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 10, 33, 215, 303
BssMI GATC 2 cut(s) 345, 561
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 3 cut(s) 33, 215, 303
Bst2UI CCWGG 2 cut(s) 11, 318
Bst4CI ACNGT 3 cut(s) 293, 352, 398
Bst6I CTCTTC 2 cut(s) 285, 522
BstACI GRCGYC 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 141, 226, 366
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 2 cut(s) 348, 564
BstMAI GTCTC 2 cut(s) 207, 392
BstMBI GATC 2 cut(s) 345, 561
BstMWI GCNNNNNNNGC 4 cut(s) 35, 155, 164, 269
BstNI CCWGG 2 cut(s) 11, 318
BstNSI RCATGY 2 cut(s) 179, 373
BstSCI CCNGG 2 cut(s) 9, 316
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 2 cut(s) 104, 381
BstV1I GCAGC 4 cut(s) 13, 16, 250, 436
Bsu36I CCTNAGG 1 cut(s) 516
BsuRI GGCC 1 cut(s) 415
BtgI CCRYGG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 3 cut(s) 141, 226, 366
Cac8I GCNNGC 1 cut(s) 177
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 3 cut(s) 224, 413, 512
Csp6I GTAC 1 cut(s) 585
CviAII CATG 5 cut(s) 34, 176, 197, 370, 541
CviJI RGCY 8 cut(s) 9, 26, 38, 123, 167, 340, 415, 600
CviKI_1 RGCY 8 cut(s) 9, 26, 38, 123, 167, 340, 415, 600
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 1 cut(s) 516
DpnI GATC 2 cut(s) 347, 563
DpnII GATC 2 cut(s) 345, 561
Eam1104I CTCTTC 2 cut(s) 285, 522
EarI CTCTTC 2 cut(s) 285, 522
Eco130I CCWWGG 3 cut(s) 33, 215, 303
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 224, 512
Eco57I CTGAAG 1 cut(s) 6
Eco81I CCTNAGG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 512
EcoRII CCWGG 2 cut(s) 9, 316
EcoT14I CCWWGG 3 cut(s) 33, 215, 303
EcoT22I ATGCAT 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 3 cut(s) 33, 215, 303
FaeI CATG 5 cut(s) 37, 179, 200, 373, 544
FaqI GGGAC 2 cut(s) 413, 525
FatI CATG 5 cut(s) 33, 175, 196, 369, 540
Fnu4HI GCNGC 5 cut(s) 27, 30, 264, 450, 453
FokI GGATG 3 cut(s) 148, 233, 373
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 5 cut(s) 27, 30, 264, 450, 453
FspBI CTAG 2 cut(s) 216, 341
GlaI GCGC 1 cut(s) 159
GluI GCNGC 5 cut(s) 27, 30, 264, 450, 453
GsaI CCCAGC 1 cut(s) 339
HaeIII GGCC 1 cut(s) 415
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 5 cut(s) 37, 179, 200, 373, 544
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 3 cut(s) 15, 200, 463
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 43, 379, 394
Hpy188III TCNNGA 3 cut(s) 89, 232, 358
Hpy8I GTNNAC 3 cut(s) 43, 379, 394
Hpy99I CGWCG 2 cut(s) 245, 402
HpyCH4III ACNGT 3 cut(s) 293, 352, 398
HpyCH4IV ACGT 2 cut(s) 243, 426
HpyCH4V TGCA 2 cut(s) 196, 379
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 155, 164, 269
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 243, 426
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 5 cut(s) 37, 179, 200, 373, 544
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 2 cut(s) 345, 561
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 9 cut(s) 19, 23, 102, 282, 303, 321, 330, 420, 473
Lsp1109I GCAGC 4 cut(s) 13, 16, 250, 436
MaeI CTAG 2 cut(s) 216, 341
MaeII ACGT 2 cut(s) 243, 426
MaeIII GTNAC 1 cut(s) 16
MalI GATC 2 cut(s) 347, 563
MboI GATC 2 cut(s) 345, 561
MboII GAAGA 3 cut(s) 272, 539, 557
MhlI GDGCHC 3 cut(s) 104, 125, 381
MluCI AATT 1 cut(s) 548
MlyI GAGTC 2 cut(s) 24, 209
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 2 cut(s) 523, 530
Mph1103I ATGCAT 1 cut(s) 198
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 11, 318
MvaI CCWGG 2 cut(s) 11, 318
MwoI GCNNNNNNNGC 4 cut(s) 35, 155, 164, 269
NcoI CCATGG 1 cut(s) 33
NdeII GATC 2 cut(s) 345, 561
NlaIII CATG 5 cut(s) 37, 179, 200, 373, 544
NlaIV GGNNCC 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 16
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 2 cut(s) 179, 373
PaeI GCATGC 1 cut(s) 179
PciI ACATGT 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 285
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 387
PfeI GAWTC 1 cut(s) 463
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 5 cut(s) 28, 31, 265, 451, 454
PleI GAGTC 2 cut(s) 23, 208
PpsI GAGTC 2 cut(s) 23, 208
PpuMI RGGWCCY 1 cut(s) 512
PscI ACATGT 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 9, 316
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 9, 316
PspN4I GGNNCC 1 cut(s) 513
PspPI GGNCC 3 cut(s) 224, 413, 512
PspPPI RGGWCCY 1 cut(s) 512
PsrI GAACNNNNNNTAC 2 cut(s) 488, 520
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 1 cut(s) 374
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 5 cut(s) 27, 30, 264, 450, 453
Sau3AI GATC 2 cut(s) 345, 561
Sau96I GGNCC 3 cut(s) 224, 413, 512
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 2 cut(s) 24, 209
ScrFI CCNGG 2 cut(s) 11, 318
SduI GDGCHC 3 cut(s) 104, 125, 381
SetI ASST 9 cut(s) 28, 155, 169, 217, 246, 429, 517, 534, 602
SfcI CTRYAG 1 cut(s) 46
SinI GGWCC 2 cut(s) 224, 512
SmiMI CAYNNNNRTG 1 cut(s) 374
SphI GCATGC 1 cut(s) 179
Sse9I AATT 1 cut(s) 548
SsiI CCGC 2 cut(s) 453, 568
SspMI CTAG 2 cut(s) 216, 341
StyD4I CCNGG 2 cut(s) 9, 316
StyI CCWWGG 3 cut(s) 33, 215, 303
TaaI ACNGT 3 cut(s) 293, 352, 398
TaiI ACGT 2 cut(s) 246, 429
TaqI TCGA 3 cut(s) 211, 231, 400
TasI AATT 1 cut(s) 548
TatI WGTACW 1 cut(s) 584
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 1 cut(s) 463
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 4 cut(s) 26, 29, 263, 449
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 2 cut(s) 171, 455
VneI GTGCAC 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 224, 512
XceI RCATGY 2 cut(s) 179, 373
XmaJI CCTAGG 1 cut(s) 215
XspI CTAG 2 cut(s) 216, 341
ZraI GACGTC 1 cut(s) 427
ZrmI AGTACT 1 cut(s) 586
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.