Rh7BG162600

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001923
Physical Location & Seq
Forward (+)
12578804 .. 12581251
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG162600.1

Sequence Viewer

Length: 879 bp
ATGGCCACCGGAGTTTCGGTTGCCTCAATCCCAATATGGTACTGTTTCGGCCGAAACAATCTACTTGCTCGAAAACCCATCTCCCAAACAAGCACCCCCTGTTTTGTTTTGGCTTCAGCAAAGAAGAGACACTTGGTTATGGAAAAGAGAACAGAGCATGAATTTACAGAGAAAAGCTTGGAACAAACAGAGAGAAGGAGTGTTATAACTCCAAGAGTTGCAGAGAGATTAGCAAGAAGGAAATCGGAGAGAAATACTTACCTTGTTGCTGCAATCATGTCCAGCCTGGGAGTCACTTCAGCTGTTGCCATGGCTGTTTACTACAGATTTTCTTGGCAAACAGAGATTGGGGAGTTTCTTGTTCCAGAGATGTTGGGCACATTTTTGCTCTCTGTGGGCTCTGCTGTGGGGATGGAGTTTTGGGCAAGGTGGGCGCACAGAGCTCTGTGGCATGCCTCATTATGGCAGATGCATGAGTCTCACCATCGACCTAGGGATGGTCCTTTCGAGATTAACGACGTGTTTGCTATAACCAATGCTGCTCCTGCTATTGCTCTTCTCTCTTACGGTTTCTTTACCAAGGGTCTTTTTCCTGGACTATGTTTTGGAGCTGGGCTAGGGATCACAGTTTTCGGGATGGCATACATGTTTGTGCACGATGGTCTCGTCCACCGTCGATTTCCAGTCGGGCCAATCGCAGACGTCCCTTATTTACAAAGAGTTGCTGCCGCCCACCAGGTTCATCATACATACATATTGAATGGAGTGCCGTATGGGTTGTTCTTGGGACCTAAGGAAATAGAAGAGGTTGGAGGCATGGAAGAATTAGAAAAGGAGATCAAGCGGAGAAGTACCAAGAGTACTATACCCAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

292

Amino Acids

32.78

Weight (kDa)

9.46

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 135 - 261 2.2e-14 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AatII GACGTC 1 cut(s) 705
AciI CCGC 2 cut(s) 729, 844
AclWI GGATC 1 cut(s) 629
AcoI YGGCCR 2 cut(s) 3, 49
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 2 cut(s) 99, 282
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 3 cut(s) 41, 853, 862
AfiI CCNNNNNNNGG 2 cut(s) 462, 497
AflIII ACRYGT 2 cut(s) 519, 645
AgsI TTSAA 1 cut(s) 760
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 3 cut(s) 285, 592, 735
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AluBI AGCT 5 cut(s) 177, 302, 443, 611, 876
AluI AGCT 5 cut(s) 177, 302, 443, 611, 876
Alw21I GWGCWC 2 cut(s) 445, 657
Alw26I GTCTC 3 cut(s) 121, 483, 668
Alw44I GTGCAC 1 cut(s) 653
AlwI GGATC 1 cut(s) 629
AoxI GGCC 3 cut(s) 3, 49, 689
ApaLI GTGCAC 1 cut(s) 653
ApeKI GCWGC 3 cut(s) 269, 539, 725
ApoI RAATTY 1 cut(s) 161
AspA2I CCTAGG 1 cut(s) 491
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 3 cut(s) 500, 689, 788
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 2 cut(s) 500, 788
AvrII CCTAGG 1 cut(s) 491
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 2 cut(s) 380, 657
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 401, 445
Bbv12I GWGCWC 2 cut(s) 445, 657
BbvI GCAGC 3 cut(s) 256, 526, 712
BccI CCATC 6 cut(s) 86, 406, 491, 492, 631, 653
BceAI ACGGC 1 cut(s) 754
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 3 cut(s) 287, 594, 737
BcoDI GTCTC 3 cut(s) 121, 483, 668
BfaI CTAG 2 cut(s) 492, 617
BfmI CTRYAG 1 cut(s) 322
BisI GCNGC 4 cut(s) 270, 540, 726, 729
BlnI CCTAGG 1 cut(s) 491
BlsI GCNGC 4 cut(s) 271, 541, 727, 730
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 3 cut(s) 287, 594, 737
Bme18I GGWCC 2 cut(s) 500, 788
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 3 cut(s) 500, 689, 788
BmiI GGNNCC 1 cut(s) 789
BmrFI CCNGG 3 cut(s) 287, 594, 737
BmsI GCATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 4 cut(s) 286, 309, 491, 579
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 497
Bse1I ACTGG 1 cut(s) 683
Bse21I CCTNAGG 1 cut(s) 792
BseBI CCWGG 3 cut(s) 287, 594, 737
BseDI CCNNGG 4 cut(s) 286, 309, 491, 579
BseGI GGATG 3 cut(s) 417, 502, 642
BseLI CCNNNNNNNGG 2 cut(s) 462, 497
BseNI ACTGG 1 cut(s) 683
BseSI GKGCMC 2 cut(s) 380, 657
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 256, 526, 712
BseYI CCCAGC 1 cut(s) 611
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 3 cut(s) 5, 51, 691
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 2 cut(s) 445, 657
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 2 cut(s) 689, 801
BslI CCNNNNNNNGG 2 cut(s) 462, 497
BsmAI GTCTC 3 cut(s) 121, 483, 668
BsmFI GGGAC 2 cut(s) 689, 801
BsnI GGCC 3 cut(s) 5, 51, 691
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 4 cut(s) 380, 401, 445, 657
Bsp143I GATC 2 cut(s) 621, 837
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 2 cut(s) 729, 844
BspANI GGCC 3 cut(s) 5, 51, 691
BspLI GGNNCC 1 cut(s) 789
BspPI GGATC 1 cut(s) 629
BspQI GCTCTTC 1 cut(s) 561
BspTNI GGTCTC 1 cut(s) 668
BsrI ACTGG 1 cut(s) 683
BssECI CCNNGG 4 cut(s) 286, 309, 491, 579
BssMI GATC 2 cut(s) 621, 837
BssNI GRCGYC 1 cut(s) 702
BssT1I CCWWGG 3 cut(s) 309, 491, 579
Bst2UI CCWGG 3 cut(s) 287, 594, 737
Bst4CI ACNGT 4 cut(s) 44, 569, 628, 674
Bst6I CTCTTC 3 cut(s) 119, 561, 798
BstACI GRCGYC 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 1 cut(s) 792
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 417, 502, 642
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 2 cut(s) 624, 840
BstMAI GTCTC 3 cut(s) 121, 483, 668
BstMBI GATC 2 cut(s) 621, 837
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 3 cut(s) 431, 440, 545
BstNI CCWGG 3 cut(s) 287, 594, 737
BstNSI RCATGY 2 cut(s) 455, 649
BstSCI CCNGG 3 cut(s) 285, 592, 735
BstSFI CTRYAG 1 cut(s) 322
BstSLI GKGCMC 2 cut(s) 380, 657
BstV1I GCAGC 3 cut(s) 256, 526, 712
BstZI CGGCCG 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 3 cut(s) 5, 51, 691
BtgI CCRYGG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 417, 502, 642
Cac8I GCNNGC 1 cut(s) 453
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 500, 689, 788
CsiI ACCWGGT 1 cut(s) 735
Csp6I GTAC 3 cut(s) 40, 852, 861
CviAII CATG 7 cut(s) 158, 277, 310, 452, 473, 646, 817
CviQI GTAC 3 cut(s) 40, 852, 861
DdeI CTNAG 1 cut(s) 792
DpnI GATC 2 cut(s) 623, 839
DpnII GATC 2 cut(s) 621, 837
EaeI YGGCCR 2 cut(s) 3, 49
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 3 cut(s) 119, 561, 798
EarI CTCTTC 3 cut(s) 119, 561, 798
Ecl136II GAGCTC 1 cut(s) 443
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 309, 491, 579
Eco24I GRGCYC 2 cut(s) 401, 445
Eco31I GGTCTC 1 cut(s) 668
Eco47I GGWCC 2 cut(s) 500, 788
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 443
Eco57I CTGAAG 2 cut(s) 99, 282
Eco81I CCTNAGG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 788
EcoRII CCWGG 3 cut(s) 285, 592, 735
EcoT14I CCWWGG 3 cut(s) 309, 491, 579
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 401, 445
ErhI CCWWGG 3 cut(s) 309, 491, 579
FaeI CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
FalI AAGNNNNNCTT 2 cut(s) 116, 148
FaqI GGGAC 2 cut(s) 689, 801
FatI CATG 7 cut(s) 157, 276, 309, 451, 472, 645, 816
Fnu4HI GCNGC 4 cut(s) 270, 540, 726, 729
FokI GGATG 3 cut(s) 424, 509, 649
FriOI GRGCYC 2 cut(s) 401, 445
Fsp4HI GCNGC 4 cut(s) 270, 540, 726, 729
FspBI CTAG 2 cut(s) 492, 617
GlaI GCGC 1 cut(s) 435
GluI GCNGC 4 cut(s) 270, 540, 726, 729
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 3 cut(s) 5, 51, 691
HapII CCGG 1 cut(s) 9
HhaI GCGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 2 cut(s) 291, 476
HpaII CCGG 1 cut(s) 9
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 3 cut(s) 319, 655, 670
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 3 cut(s) 365, 508, 634
Hpy8I GTNNAC 3 cut(s) 319, 655, 670
Hpy99I CGWCG 2 cut(s) 521, 678
HpyAV CCTTC 2 cut(s) 189, 231
HpyCH4III ACNGT 4 cut(s) 44, 569, 628, 674
HpyCH4IV ACGT 2 cut(s) 519, 702
HpyCH4V TGCA 4 cut(s) 221, 272, 472, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 431, 440, 545
HpyF3I CTNAG 1 cut(s) 792
HpySE526I ACGT 2 cut(s) 519, 702
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 2 cut(s) 621, 837
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 547, 608
Lsp1109I GCAGC 3 cut(s) 256, 526, 712
LweI GCATC 1 cut(s) 459
MabI ACCWGGT 1 cut(s) 735
MaeI CTAG 2 cut(s) 492, 617
MaeII ACGT 2 cut(s) 519, 702
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 623, 839
MboI GATC 2 cut(s) 621, 837
MboII GAAGA 4 cut(s) 136, 548, 815, 833
MhlI GDGCHC 4 cut(s) 380, 401, 445, 657
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 161, 824
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 300, 485
MmeI TCCRAC 1 cut(s) 790
MnlI CCTC 4 cut(s) 34, 466, 799, 806
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 474
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 513
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 3 cut(s) 287, 594, 737
MvaI CCWGG 3 cut(s) 287, 594, 737
MwoI GCNNNNNNNGC 3 cut(s) 431, 440, 545
NcoI CCATGG 1 cut(s) 309
NdeII GATC 2 cut(s) 621, 837
NlaIII CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
NlaIV GGNNCC 1 cut(s) 789
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 2 cut(s) 455, 649
PaeI GCATGC 1 cut(s) 455
PciI ACATGT 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 561
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 663
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 4 cut(s) 271, 541, 727, 730
PleI GAGTC 2 cut(s) 299, 484
PpsI GAGTC 2 cut(s) 299, 484
PpuMI RGGWCCY 1 cut(s) 788
PscI ACATGT 1 cut(s) 645
PsiI TTATAA 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 445
Psp5II RGGWCCY 1 cut(s) 788
Psp6I CCWGG 3 cut(s) 285, 592, 735
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 285, 592, 735
PspN4I GGNNCC 1 cut(s) 789
PspPI GGNCC 3 cut(s) 500, 689, 788
PspPPI RGGWCCY 1 cut(s) 788
PsrI GAACNNNNNNTAC 2 cut(s) 764, 796
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 3 cut(s) 41, 853, 862
RsaNI GTAC 3 cut(s) 40, 852, 861
RseI CAYNNNNRTG 1 cut(s) 650
SacI GAGCTC 1 cut(s) 445
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 4 cut(s) 270, 540, 726, 729
Sau3AI GATC 2 cut(s) 621, 837
Sau96I GGNCC 3 cut(s) 500, 689, 788
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 2 cut(s) 300, 485
ScrFI CCNGG 3 cut(s) 287, 594, 737
SduI GDGCHC 4 cut(s) 380, 401, 445, 657
SexAI ACCWGGT 1 cut(s) 735
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 322
SinI GGWCC 2 cut(s) 500, 788
SmiMI CAYNNNNRTG 1 cut(s) 650
SphI GCATGC 1 cut(s) 455
Sse9I AATT 2 cut(s) 161, 824
SsiI CCGC 2 cut(s) 729, 844
SspMI CTAG 2 cut(s) 492, 617
SstI GAGCTC 1 cut(s) 445
StyD4I CCNGG 3 cut(s) 285, 592, 735
StyI CCWWGG 3 cut(s) 309, 491, 579
TaaI ACNGT 4 cut(s) 44, 569, 628, 674
TaiI ACGT 2 cut(s) 522, 705
TaqI TCGA 4 cut(s) 70, 487, 507, 676
TasI AATT 2 cut(s) 161, 824
TatI WGTACW 1 cut(s) 860
TauI GCSGC 1 cut(s) 731
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 269, 539, 725
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 174, 731
VneI GTGCAC 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 500, 788
XapI RAATTY 1 cut(s) 161
XceI RCATGY 2 cut(s) 455, 649
XmaJI CCTAGG 1 cut(s) 491
XspI CTAG 2 cut(s) 492, 617
ZraI GACGTC 1 cut(s) 703
ZrmI AGTACT 1 cut(s) 862
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.