pycom06g08510

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001923
Physical Location & Seq
Reverse (-)
13723730 .. 13725304
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08510.3

Sequence Viewer

Length: 918 bp
ATGGCGACCGGAGTTTCGGTGTATTCAGTCACCTTAACTAACAGTTTCGAACGAAAAACTCCATTTTCTGGAAAACCCATCTCAGAAACGAGCCCCTCTTTGCTTTTAGCTTCTTCATCAATTCACTTCAACAGGAAGAGATGGAAGAGCATGCGCCCAGTCATGGAGAAGAAAGCGGAGAATGATCATGCAAAAGTTAAGGACGACATGACTGTGACTAAAGCCTTGGAAGACACGGAGAAGTTGAATATTATAACACTGCGTGCGTCGGAGAGATTGGAGAGGAAGAGATCAGAGAGGTATACTTACCTTGTTGCTGCAATCATGTCCAGCCTTGGAGTCACTTCAGCTGCTGCCATGGCTGTTTACTATAGATTTTATTGGCAAACAGAGGTTGGTGAATATCTTCTTCCTGAAATGTTTGGAACATTTGCTCTCTCTGTGGGATCTGCTGTGGGGATGGAGTTTTGGGCAAGGTGGGCTCATAGAGCTCTGTGGCATGCTTCACTGTGGCAGATGCATGAGTCTCACCACCGGCCCCGGGATGGTCCTTTTGAGTTGAACGATGTATTTGCAATAACCAATGCTGTTCCTGCTATTGCCCTTCTTTCTTATGGTTTGTTTAACAAGGGTCTATTTCCAGGACTCTGTTTTGGTGCTGGTTTAGGAATCACAACATTTGGGATGGCCTACATGTTTGTGCACGATGGACTTGTCCATCGTCGATTTCCGGTAGGGCCAATCGCAGATGTTCCTTATTTACGAAAAGTTGCTGCAGCCCACCAGCTTCATCACTCATGCATCTTCAATGGAGTCCCATATGGGTTGTTCTTGGGACCTAAGGAAATAAAGGAAGTTGGGGGCATGGAAGAATTGGAAAAGGAAATCCAGCGCATGGCTAGGAAATCCAACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

306

Amino Acids

34.3

Weight (kDa)

9.3

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AccB7I CCANNNNNTGG 2 cut(s) 68, 895
AccI GTMKAC 1 cut(s) 302
AciI CCGC 1 cut(s) 176
AclWI GGATC 1 cut(s) 454
AcuI CTGAAG 1 cut(s) 330
AdeI CACNNNGTG 1 cut(s) 263
AfiI CCNNNNNNNGG 5 cut(s) 68, 163, 541, 545, 895
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 4 cut(s) 130, 247, 562, 808
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 4 cut(s) 110, 350, 491, 787
AluI AGCT 4 cut(s) 110, 350, 491, 787
Alw21I GWGCWC 2 cut(s) 493, 705
Alw26I GTCTC 1 cut(s) 531
Alw44I GTGCAC 1 cut(s) 701
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 353
Ama87I CYCGRG 1 cut(s) 540
AoxI GGCC 3 cut(s) 536, 687, 737
ApaLI GTGCAC 1 cut(s) 701
ApeKI GCWGC 5 cut(s) 317, 350, 353, 773, 776
ArsI GACNNNNNNTTYG 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 2 cut(s) 156, 894
AspS9I GGNCC 4 cut(s) 537, 548, 737, 836
AsuC2I CCSGG 2 cut(s) 541, 542
AsuHPI GGTGA 3 cut(s) 22, 410, 521
AsuII TTCGAA 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 2 cut(s) 548, 836
AxyI CCTNAGG 1 cut(s) 840
BaeGI GKGCMC 1 cut(s) 705
BanII GRGCYC 3 cut(s) 95, 484, 493
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 493, 705
BbvI GCAGC 5 cut(s) 304, 337, 340, 760, 788
BccI CCATC 7 cut(s) 86, 135, 454, 539, 679, 701, 726
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 1 cut(s) 642
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 2 cut(s) 541, 542
BcoDI GTCTC 1 cut(s) 531
BfaI CTAG 1 cut(s) 900
BfmI CTRYAG 2 cut(s) 370, 774
BisI GCNGC 5 cut(s) 318, 351, 354, 774, 777
BlsI GCNGC 5 cut(s) 319, 352, 355, 775, 778
Bme1390I CCNGG 3 cut(s) 541, 542, 642
Bme18I GGWCC 2 cut(s) 548, 836
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 4 cut(s) 537, 548, 737, 836
BmiI GGNNCC 2 cut(s) 539, 837
BmrFI CCNGG 3 cut(s) 541, 542, 642
BmrI ACTGGG 1 cut(s) 152
BmsI GCATC 2 cut(s) 507, 810
BmuI ACTGGG 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 48
BpuMI CCSGG 2 cut(s) 541, 542
BsaJI CCNNGG 5 cut(s) 225, 334, 357, 539, 540
BsaWI WCCGGW 2 cut(s) 8, 730
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 163, 541, 545, 895
Bse118I RCCGGY 1 cut(s) 534
Bse1I ACTGG 1 cut(s) 158
Bse21I CCTNAGG 1 cut(s) 840
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 5 cut(s) 225, 334, 357, 539, 540
BseGI GGATG 3 cut(s) 465, 550, 690
BseLI CCNNNNNNNGG 5 cut(s) 68, 163, 541, 545, 895
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 5 cut(s) 304, 337, 340, 760, 788
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 3 cut(s) 538, 689, 739
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 493, 705
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 4 cut(s) 9, 535, 541, 731
BslFI GGGAC 2 cut(s) 800, 849
BslI CCNNNNNNNGG 5 cut(s) 68, 163, 541, 545, 895
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 2 cut(s) 800, 849
BsnI GGCC 3 cut(s) 538, 689, 739
BsoBI CYCGRG 1 cut(s) 540
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 4 cut(s) 95, 484, 493, 705
Bsp143I GATC 3 cut(s) 184, 290, 446
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 1 cut(s) 176
BspANI GGCC 3 cut(s) 538, 689, 739
BspCNI CTCAG 1 cut(s) 95
BspLI GGNNCC 2 cut(s) 539, 837
BspMAI CTGCAG 1 cut(s) 778
BspPI GGATC 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 534
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 5 cut(s) 225, 334, 357, 539, 540
BssMI GATC 3 cut(s) 184, 290, 446
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 225, 334, 357
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 4 cut(s) 44, 214, 510, 914
Bst6I CTCTTC 3 cut(s) 131, 140, 281
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 3 cut(s) 152, 264, 501
BstDEI CTNAG 2 cut(s) 82, 840
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 465, 550, 690
BstHHI GCGC 2 cut(s) 156, 894
BstKTI GATC 3 cut(s) 187, 293, 449
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 3 cut(s) 184, 290, 446
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 359, 479, 488, 593
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 3 cut(s) 154, 503, 697
BstSCI CCNGG 3 cut(s) 539, 540, 640
BstSFI CTRYAG 2 cut(s) 370, 774
BstSLI GKGCMC 1 cut(s) 705
BstV1I GCAGC 5 cut(s) 304, 337, 340, 760, 788
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BstZ17I GTATAC 1 cut(s) 303
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 3 cut(s) 538, 689, 739
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 465, 550, 690
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 257, 506
Cac8I GCNNGC 3 cut(s) 152, 264, 501
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 2 cut(s) 156, 894
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 4 cut(s) 537, 548, 737, 836
Cfr9I CCCGGG 1 cut(s) 540
CseI GACGC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 82, 840
DpnI GATC 3 cut(s) 186, 292, 448
DpnII GATC 3 cut(s) 184, 290, 446
DraIII CACNNNGTG 1 cut(s) 263
Eam1104I CTCTTC 3 cut(s) 131, 140, 281
EarI CTCTTC 3 cut(s) 131, 140, 281
Ecl136II GAGCTC 1 cut(s) 491
Eco130I CCWWGG 3 cut(s) 225, 334, 357
Eco24I GRGCYC 3 cut(s) 95, 484, 493
Eco47I GGWCC 2 cut(s) 548, 836
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 1 cut(s) 540
EcoICRI GAGCTC 1 cut(s) 491
EcoO109I RGGNCCY 1 cut(s) 836
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 3 cut(s) 225, 334, 357
EcoT22I ATGCAT 2 cut(s) 522, 803
EcoT38I GRGCYC 3 cut(s) 95, 484, 493
ErhI CCWWGG 3 cut(s) 225, 334, 357
FaqI GGGAC 2 cut(s) 800, 849
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 5 cut(s) 318, 351, 354, 774, 777
FokI GGATG 3 cut(s) 472, 557, 697
FriOI GRGCYC 3 cut(s) 95, 484, 493
Fsp4HI GCNGC 5 cut(s) 318, 351, 354, 774, 777
FspBI CTAG 1 cut(s) 900
GlaI GCGC 2 cut(s) 155, 893
GluI GCNGC 5 cut(s) 318, 351, 354, 774, 777
HaeIII GGCC 3 cut(s) 538, 689, 739
HapII CCGG 4 cut(s) 9, 535, 541, 731
HgaI GACGC 1 cut(s) 255
HhaI GCGC 2 cut(s) 156, 894
Hin6I GCGC 2 cut(s) 154, 892
HinP1I GCGC 2 cut(s) 154, 892
HinfI GANTC 5 cut(s) 339, 524, 645, 669, 813
HpaII CCGG 4 cut(s) 9, 535, 541, 731
HphI GGTGA 3 cut(s) 22, 410, 521
Hpy166II GTNNAC 3 cut(s) 303, 367, 703
Hpy188I TCNGA 3 cut(s) 85, 271, 295
Hpy188III TCNNGA 2 cut(s) 69, 413
Hpy8I GTNNAC 3 cut(s) 303, 367, 703
Hpy99I CGWCG 2 cut(s) 271, 726
HpyAV CCTTC 1 cut(s) 614
HpyCH4III ACNGT 4 cut(s) 44, 214, 510, 914
HpyCH4V TGCA 7 cut(s) 191, 320, 520, 575, 703, 776, 801
HpyF10VI GCNNNNNNNGC 4 cut(s) 359, 479, 488, 593
HpyF3I CTNAG 2 cut(s) 82, 840
HspAI GCGC 2 cut(s) 154, 892
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 184, 290, 446
LguI GCTCTTC 1 cut(s) 140
Lsp1109I GCAGC 5 cut(s) 304, 337, 340, 760, 788
LweI GCATC 2 cut(s) 507, 810
MaeI CTAG 1 cut(s) 900
MaeIII GTNAC 3 cut(s) 28, 214, 340
MalI GATC 3 cut(s) 186, 292, 448
MboI GATC 3 cut(s) 184, 290, 446
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 4 cut(s) 95, 484, 493, 705
MluCI AATT 2 cut(s) 120, 872
MlyI GAGTC 4 cut(s) 348, 533, 639, 822
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 4 cut(s) 106, 276, 291, 385
Mph1103I ATGCAT 2 cut(s) 522, 803
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 3 cut(s) 35, 198, 624
MslI CAYNNNNRTG 2 cut(s) 212, 698
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 4 cut(s) 9, 535, 541, 731
MspR9I CCNGG 3 cut(s) 541, 542, 642
MvaI CCWGG 1 cut(s) 642
MwoI GCNNNNNNNGC 4 cut(s) 359, 479, 488, 593
NciI CCSGG 2 cut(s) 541, 542
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 820
NdeII GATC 3 cut(s) 184, 290, 446
NlaIV GGNNCC 2 cut(s) 539, 837
NmuCI GTSAC 3 cut(s) 28, 214, 340
NsiI ATGCAT 2 cut(s) 522, 803
NspI RCATGY 3 cut(s) 154, 503, 697
NspV TTCGAA 1 cut(s) 48
PaeI GCATGC 2 cut(s) 154, 503
PciI ACATGT 1 cut(s) 693
PciSI GCTCTTC 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 669
PflMI CCANNNNNTGG 2 cut(s) 68, 895
PfoI TCCNGGA 1 cut(s) 640
PkrI GCNGC 5 cut(s) 319, 352, 355, 775, 778
PleI GAGTC 4 cut(s) 347, 532, 639, 821
PpsI GAGTC 4 cut(s) 347, 532, 639, 821
PpuMI RGGWCCY 1 cut(s) 836
PscI ACATGT 1 cut(s) 693
PsiI TTATAA 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 493
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 1 cut(s) 640
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 2 cut(s) 539, 837
PspPI GGNCC 4 cut(s) 537, 548, 737, 836
PspPPI RGGWCCY 1 cut(s) 836
PstI CTGCAG 1 cut(s) 778
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 350
RseI CAYNNNNRTG 2 cut(s) 212, 698
SacI GAGCTC 1 cut(s) 493
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 3 cut(s) 35, 198, 624
SatI GCNGC 5 cut(s) 318, 351, 354, 774, 777
Sau3AI GATC 3 cut(s) 184, 290, 446
Sau96I GGNCC 4 cut(s) 537, 548, 737, 836
SchI GAGTC 4 cut(s) 348, 533, 639, 822
ScrFI CCNGG 3 cut(s) 541, 542, 642
SduI GDGCHC 4 cut(s) 95, 484, 493, 705
SfaNI GCATC 2 cut(s) 507, 810
SfcI CTRYAG 2 cut(s) 370, 774
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 2 cut(s) 548, 836
SmaI CCCGGG 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 212, 698
SphI GCATGC 2 cut(s) 154, 503
Sse9I AATT 2 cut(s) 120, 872
SsiI CCGC 1 cut(s) 176
SspI AATATT 1 cut(s) 250
SspMI CTAG 1 cut(s) 900
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 3 cut(s) 539, 540, 640
StyI CCWWGG 3 cut(s) 225, 334, 357
TaaI ACNGT 4 cut(s) 44, 214, 510, 914
TaqI TCGA 2 cut(s) 48, 724
TasI AATT 2 cut(s) 120, 872
TfiI GAWTC 1 cut(s) 669
Tru1I TTAA 3 cut(s) 35, 198, 624
Tru9I TTAA 3 cut(s) 35, 198, 624
TscAI CASTG 2 cut(s) 264, 513
TseFI GTSAC 3 cut(s) 28, 214, 340
TseI GCWGC 5 cut(s) 317, 350, 353, 773, 776
Tsp45I GTSAC 3 cut(s) 28, 214, 340
TspDTI ATGAA 2 cut(s) 105, 779
TspGWI ACGGA 1 cut(s) 251
TspMI CCCGGG 1 cut(s) 540
TspRI CASTG 2 cut(s) 264, 513
Van91I CCANNNNNTGG 2 cut(s) 68, 895
VneI GTGCAC 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 548, 836
XceI RCATGY 3 cut(s) 154, 503, 697
XmaI CCCGGG 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 1 cut(s) 900
Zsp2I ATGCAT 2 cut(s) 522, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.