MD06G1089200.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001923
Physical Location & Seq
Reverse (-)
21735483 .. 21737612
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1089200.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGCGACCGGAGTTTCGGTGTCTTCAGTCACCTTAACAAACAGTTTCGAACGAAAAACTCCGTTTTCTGGAAAACCCTTCTCAGAAACGAGCCCCTCTTCGCTTTTAGCTTCTTCGTCAATTCACTTCAACAGGAAGAGATGGAAGAGCATGCGCTCAGTCATGGAGAAGAAAGTGGAGAATGATCATGCACAACTTAAGGACGACATGACTGTGACCAAAGCCTTGGAAGACACGGAGAAGTGGAATATTATAACGCTGCGTGCGTCGGAGAGATTGGAGAGGAAGAGATCAGAGAGGTATACTTACCTTGTTGCTGCAATCATGTCCAGCCTTGGAGTCACTTCAGCTGCTGCCATGGCTGTTTACTATAGATTTTCTTGGCAAACAGAGGTTGGTGAATATCTTCTTCCTGAAATGTTTGGAACATTTGCTCTCTCTGTGGGATCTGCTGTGGGGATGGAGTTTTGGGCAAGGTGGGCTCATAGAACTCTGTGGCATGCTTCACTGTGGCAGATGCATGAGTCTCACCACCGGCCCCGGGATGGTCCTTTTGAGTTGAACGATGTATTTGCAATAACCAATGCTGTTCCTGCTATTGCCCTTCTTTCTTATGGTTTGTTTAACAAGGGTCTATTTCCAGGACTCTGTTTTGGTGCTGGTTTAGGAATCACAACGTTTGGGATGGCCTATATGTTTGTGCACGATGGACTTGTCCACCGTCGATTTCCGGTAGGGCCCATCGCAGATGTCCCTTATTTACGAAAAGTTGCTGCAGCCCACCAGCTTCATCACTCATGCATCTTCAATGGAGTCCCATATGGGTTGTTCTTGGGACCTAAGGAAATAAGGGAAGTTGGGGGCATGGAAGAATTGGAAAAGGAAGTCCAGCGCAGAGCTAAGAAATCCAACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

306

Amino Acids

34.3

Weight (kDa)

9.35

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 151 - 277 3.6e-14 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AccI GTMKAC 1 cut(s) 302
AclI AACGTT 1 cut(s) 677
AclWI GGATC 1 cut(s) 454
AcuI CTGAAG 2 cut(s) 9, 330
AfiI CCNNNNNNNGG 3 cut(s) 68, 541, 545
AflII CTTAAG 1 cut(s) 197
AgsI TTSAA 3 cut(s) 130, 562, 808
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 4 cut(s) 110, 350, 787, 899
AluI AGCT 4 cut(s) 110, 350, 787, 899
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 1 cut(s) 531
Alw44I GTGCAC 1 cut(s) 701
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 353
Ama87I CYCGRG 1 cut(s) 540
AoxI GGCC 3 cut(s) 536, 687, 737
ApaI GGGCCC 1 cut(s) 741
ApaLI GTGCAC 1 cut(s) 701
ApeKI GCWGC 6 cut(s) 259, 317, 350, 353, 773, 776
ArsI GACNNNNNNTTYG 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 2 cut(s) 156, 894
AspS9I GGNCC 5 cut(s) 537, 548, 737, 738, 836
AsuC2I CCSGG 2 cut(s) 541, 542
AsuHPI GGTGA 3 cut(s) 22, 410, 521
AsuII TTCGAA 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 2 cut(s) 548, 836
AxyI CCTNAGG 1 cut(s) 840
BaeGI GKGCMC 2 cut(s) 705, 741
BanII GRGCYC 3 cut(s) 95, 484, 741
BbsI GAAGAC 2 cut(s) 15, 237
Bbv12I GWGCWC 1 cut(s) 705
BbvI GCAGC 6 cut(s) 246, 304, 337, 340, 760, 788
BccI CCATC 6 cut(s) 135, 454, 539, 679, 701, 749
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 1 cut(s) 642
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 2 cut(s) 541, 542
BcoDI GTCTC 1 cut(s) 531
BfmI CTRYAG 2 cut(s) 370, 774
BfrI CTTAAG 1 cut(s) 197
BisI GCNGC 6 cut(s) 260, 318, 351, 354, 774, 777
BlsI GCNGC 6 cut(s) 261, 319, 352, 355, 775, 778
Bme1390I CCNGG 3 cut(s) 541, 542, 642
Bme18I GGWCC 2 cut(s) 548, 836
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 5 cut(s) 537, 548, 737, 738, 836
BmiI GGNNCC 3 cut(s) 539, 739, 837
BmrFI CCNGG 3 cut(s) 541, 542, 642
BmsI GCATC 2 cut(s) 507, 810
BpiI GAAGAC 2 cut(s) 15, 237
Bpu14I TTCGAA 1 cut(s) 48
BpuMI CCSGG 2 cut(s) 541, 542
BsaJI CCNNGG 5 cut(s) 225, 334, 357, 539, 540
BsaWI WCCGGW 2 cut(s) 8, 730
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 541, 545
Bse118I RCCGGY 1 cut(s) 534
Bse21I CCTNAGG 1 cut(s) 840
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 5 cut(s) 225, 334, 357, 539, 540
BseGI GGATG 3 cut(s) 465, 550, 690
BseLI CCNNNNNNNGG 3 cut(s) 68, 541, 545
BseMII CTCAG 2 cut(s) 96, 171
BseSI GKGCMC 2 cut(s) 705, 741
BseXI GCAGC 6 cut(s) 246, 304, 337, 340, 760, 788
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 3 cut(s) 538, 689, 739
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 4 cut(s) 9, 535, 541, 731
BslFI GGGAC 3 cut(s) 737, 800, 849
BslI CCNNNNNNNGG 3 cut(s) 68, 541, 545
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 3 cut(s) 737, 800, 849
BsnI GGCC 3 cut(s) 538, 689, 739
BsoBI CYCGRG 1 cut(s) 540
Bsp119I TTCGAA 1 cut(s) 48
Bsp120I GGGCCC 1 cut(s) 737
Bsp1286I GDGCHC 4 cut(s) 95, 484, 705, 741
Bsp143I GATC 3 cut(s) 184, 290, 446
Bsp19I CCATGG 1 cut(s) 357
BspANI GGCC 3 cut(s) 538, 689, 739
BspCNI CTCAG 2 cut(s) 95, 170
BspLI GGNNCC 3 cut(s) 539, 739, 837
BspMAI CTGCAG 1 cut(s) 778
BspPI GGATC 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 48
BspTI CTTAAG 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 5 cut(s) 225, 334, 357, 539, 540
BssMI GATC 3 cut(s) 184, 290, 446
BssNAI GTATAC 1 cut(s) 303
BssT1I CCWWGG 3 cut(s) 225, 334, 357
Bst1107I GTATAC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 5 cut(s) 44, 214, 510, 722, 914
Bst6I CTCTTC 4 cut(s) 103, 131, 140, 281
BstAFI CTTAAG 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 3 cut(s) 152, 264, 501
BstDEI CTNAG 4 cut(s) 82, 157, 840, 900
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 465, 550, 690
BstHHI GCGC 2 cut(s) 156, 894
BstKTI GATC 3 cut(s) 187, 293, 449
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 3 cut(s) 184, 290, 446
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 359, 479, 593
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 2 cut(s) 154, 503
BstSCI CCNGG 3 cut(s) 539, 540, 640
BstSFI CTRYAG 2 cut(s) 370, 774
BstSLI GKGCMC 2 cut(s) 705, 741
BstV1I GCAGC 6 cut(s) 246, 304, 337, 340, 760, 788
BstV2I GAAGAC 2 cut(s) 15, 237
BstX2I RGATCY 1 cut(s) 446
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 1 cut(s) 446
BstZ17I GTATAC 1 cut(s) 303
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 3 cut(s) 538, 689, 739
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 727
BtsCI GGATG 3 cut(s) 465, 550, 690
BtsIMutI CAGTG 1 cut(s) 506
Cac8I GCNNGC 3 cut(s) 152, 264, 501
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 2 cut(s) 156, 894
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 5 cut(s) 537, 548, 737, 738, 836
Cfr9I CCCGGG 1 cut(s) 540
CseI GACGC 1 cut(s) 255
DdeI CTNAG 4 cut(s) 82, 157, 840, 900
DpnI GATC 3 cut(s) 186, 292, 448
DpnII GATC 3 cut(s) 184, 290, 446
Eam1104I CTCTTC 4 cut(s) 103, 131, 140, 281
EarI CTCTTC 4 cut(s) 103, 131, 140, 281
Eco130I CCWWGG 3 cut(s) 225, 334, 357
Eco24I GRGCYC 3 cut(s) 95, 484, 741
Eco47I GGWCC 2 cut(s) 548, 836
Eco57I CTGAAG 2 cut(s) 9, 330
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 1 cut(s) 540
EcoO109I RGGNCCY 2 cut(s) 737, 836
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 3 cut(s) 225, 334, 357
EcoT22I ATGCAT 2 cut(s) 522, 803
EcoT38I GRGCYC 3 cut(s) 95, 484, 741
ErhI CCWWGG 3 cut(s) 225, 334, 357
FaqI GGGAC 3 cut(s) 737, 800, 849
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 6 cut(s) 260, 318, 351, 354, 774, 777
FokI GGATG 3 cut(s) 472, 557, 697
FriOI GRGCYC 3 cut(s) 95, 484, 741
Fsp4HI GCNGC 6 cut(s) 260, 318, 351, 354, 774, 777
GlaI GCGC 2 cut(s) 155, 893
GluI GCNGC 6 cut(s) 260, 318, 351, 354, 774, 777
HaeIII GGCC 3 cut(s) 538, 689, 739
HapII CCGG 4 cut(s) 9, 535, 541, 731
HgaI GACGC 1 cut(s) 255
HhaI GCGC 2 cut(s) 156, 894
Hin6I GCGC 2 cut(s) 154, 892
HinP1I GCGC 2 cut(s) 154, 892
HinfI GANTC 5 cut(s) 339, 524, 645, 669, 813
HpaII CCGG 4 cut(s) 9, 535, 541, 731
HphI GGTGA 3 cut(s) 22, 410, 521
Hpy166II GTNNAC 4 cut(s) 303, 367, 703, 718
Hpy188I TCNGA 3 cut(s) 85, 271, 295
Hpy188III TCNNGA 2 cut(s) 69, 413
Hpy8I GTNNAC 4 cut(s) 303, 367, 703, 718
Hpy99I CGWCG 2 cut(s) 271, 726
HpyAV CCTTC 2 cut(s) 88, 614
HpyCH4III ACNGT 5 cut(s) 44, 214, 510, 722, 914
HpyCH4IV ACGT 1 cut(s) 677
HpyCH4V TGCA 7 cut(s) 191, 320, 520, 575, 703, 776, 801
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 479, 593
HpyF3I CTNAG 4 cut(s) 82, 157, 840, 900
HpySE526I ACGT 1 cut(s) 677
HspAI GCGC 2 cut(s) 154, 892
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 184, 290, 446
LguI GCTCTTC 1 cut(s) 140
Lsp1109I GCAGC 6 cut(s) 246, 304, 337, 340, 760, 788
LweI GCATC 2 cut(s) 507, 810
MaeII ACGT 1 cut(s) 677
MaeIII GTNAC 3 cut(s) 28, 214, 340
MalI GATC 3 cut(s) 186, 292, 448
MboI GATC 3 cut(s) 184, 290, 446
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 4 cut(s) 95, 484, 705, 741
MluCI AATT 2 cut(s) 120, 872
MlyI GAGTC 4 cut(s) 348, 533, 639, 822
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 4 cut(s) 106, 276, 291, 385
Mph1103I ATGCAT 2 cut(s) 522, 803
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 3 cut(s) 35, 198, 624
MslI CAYNNNNRTG 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 350
MspCI CTTAAG 1 cut(s) 197
MspI CCGG 4 cut(s) 9, 535, 541, 731
MspR9I CCNGG 3 cut(s) 541, 542, 642
MvaI CCWGG 1 cut(s) 642
MwoI GCNNNNNNNGC 3 cut(s) 359, 479, 593
NciI CCSGG 2 cut(s) 541, 542
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 1 cut(s) 820
NdeII GATC 3 cut(s) 184, 290, 446
NlaIV GGNNCC 3 cut(s) 539, 739, 837
NmuCI GTSAC 3 cut(s) 28, 214, 340
NsiI ATGCAT 2 cut(s) 522, 803
NspI RCATGY 2 cut(s) 154, 503
NspV TTCGAA 1 cut(s) 48
PaeI GCATGC 2 cut(s) 154, 503
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 669
PfoI TCCNGGA 1 cut(s) 640
PkrI GCNGC 6 cut(s) 261, 319, 352, 355, 775, 778
PleI GAGTC 4 cut(s) 347, 532, 639, 821
PpsI GAGTC 4 cut(s) 347, 532, 639, 821
PpuMI RGGWCCY 1 cut(s) 836
PsiI TTATAA 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 1 cut(s) 640
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 3 cut(s) 539, 739, 837
PspOMI GGGCCC 1 cut(s) 737
PspPI GGNCC 5 cut(s) 537, 548, 737, 738, 836
PspPPI RGGWCCY 1 cut(s) 836
PstI CTGCAG 1 cut(s) 778
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 350
RseI CAYNNNNRTG 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 3 cut(s) 35, 198, 624
SatI GCNGC 6 cut(s) 260, 318, 351, 354, 774, 777
Sau3AI GATC 3 cut(s) 184, 290, 446
Sau96I GGNCC 5 cut(s) 537, 548, 737, 738, 836
SchI GAGTC 4 cut(s) 348, 533, 639, 822
ScrFI CCNGG 3 cut(s) 541, 542, 642
SduI GDGCHC 4 cut(s) 95, 484, 705, 741
SfaNI GCATC 2 cut(s) 507, 810
SfcI CTRYAG 2 cut(s) 370, 774
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 2 cut(s) 548, 836
SmaI CCCGGG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SphI GCATGC 2 cut(s) 154, 503
Sse9I AATT 2 cut(s) 120, 872
SspI AATATT 1 cut(s) 250
StyD4I CCNGG 3 cut(s) 539, 540, 640
StyI CCWWGG 3 cut(s) 225, 334, 357
TaaI ACNGT 5 cut(s) 44, 214, 510, 722, 914
TaiI ACGT 1 cut(s) 680
TaqI TCGA 2 cut(s) 48, 724
TasI AATT 2 cut(s) 120, 872
TfiI GAWTC 1 cut(s) 669
Tru1I TTAA 3 cut(s) 35, 198, 624
Tru9I TTAA 3 cut(s) 35, 198, 624
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 3 cut(s) 28, 214, 340
TseI GCWGC 6 cut(s) 259, 317, 350, 353, 773, 776
Tsp45I GTSAC 3 cut(s) 28, 214, 340
TspDTI ATGAA 1 cut(s) 779
TspGWI ACGGA 2 cut(s) 51, 251
TspMI CCCGGG 1 cut(s) 540
TspRI CASTG 1 cut(s) 513
Vha464I CTTAAG 1 cut(s) 197
VneI GTGCAC 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 548, 836
XceI RCATGY 2 cut(s) 154, 503
XmaI CCCGGG 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 405
Zsp2I ATGCAT 2 cut(s) 522, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.