MD07G1278900.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001923
Physical Location & Seq
Reverse (-)
34339931 .. 34342611
2681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1278900.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCGGTCGGACTCTTCGCCGTCACAACCCCCACCCCCTTCCGCCTAATCCACTCCTCCTCCTACCACCTCCCAAAACCCAACCCCACCACCGTCTTGTTCCCAAATCTCCGCAACCAGAAGTCAGCACTCCACAGGGCTCGCCGGAAACTCTGTTTCACCGTCTATGTAGTGATGGAGGACCAAAAACACATAACCAACCTCGACGATTCCGCCGGAAAAGCCCCGGAAGCCGCCGATTCTCAGATCCCAATTGTAATTCCGTCGGTTCGCGTGGCGGAGAGGCTGTCGAGGAAGAAATCGGAGAGGTTCGCGTATCTTGTCGCTGCCGTGATGTCCAGCTTAGGGATTACTTCCATGTCTGTCATGGCTGTCTACTACAGATTTTACTGGCAAATGGAGGGTGGGACTGTGCCAATGTCTGAAATGCTGGGTACATTTGCTCTATCTGTTGGTGCTGCTGTGGGAATGGAATTTTGGGCAAGATGGGCACATAAAGCTCTTTGGCACGCTTCCTTATGGCATATGCACGAGTCGCACCACCAACCCAGAGAAGGTCCGTTCGAGCTTAACGACGTGTTTGCGATAATCAACGCCGTTCCGGCAATAGCCCTTCTCAGTTATGGTTTCTTCCACAAAGGCCTTGTTCCTGGCCTTTGTTTTGGTGCTGGCCTTGGAATTACGGTGTTTGGGATGGCGTATATGTTTGTCCATGATGGCCTAGTGCATAAAAGATTCCCGGTGGGGCCCATAGCCGACGTGCCCTATTTGCGAAAGGTTGCTGCGGCTCATCAGCTCCACCACTCGGAGAAGTTCGAGGGTGTCCCATATGGTTTGTTTCTTGGACCTAAGGAACTCGAAGAAGTGGGAGGACTAGAAGAGTTGGATAAGGAGATCAATAGGAGAATCAAAGCACACAAGGGCTTATAA

Protein Analysis

310

Amino Acids

34.43

Weight (kDa)

9.22

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 153 - 281 2.2e-14 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 928
AccI GTMKAC 1 cut(s) 375
AccII CGCG 2 cut(s) 273, 314
AciI CCGC 7 cut(s) 5, 43, 112, 213, 234, 278, 785
AclWI GGATC 1 cut(s) 241
AcsI RAATTY 1 cut(s) 473
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 3 cut(s) 345, 554, 805
AflIII ACRYGT 1 cut(s) 576
AjiI CACGTC 2 cut(s) 577, 760
AjnI CCWGG 1 cut(s) 649
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 4 cut(s) 342, 500, 568, 796
AluI AGCT 4 cut(s) 342, 500, 568, 796
AlwI GGATC 1 cut(s) 241
AoxI GGCC 5 cut(s) 640, 652, 670, 718, 746
ApaI GGGCCC 1 cut(s) 750
ApeKI GCWGC 3 cut(s) 326, 458, 782
ApoI RAATTY 1 cut(s) 473
AspS9I GGNCC 5 cut(s) 181, 557, 746, 747, 845
AsuC2I CCSGG 2 cut(s) 227, 740
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 3 cut(s) 181, 557, 845
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 3 cut(s) 493, 750, 765
BanII GRGCYC 2 cut(s) 142, 750
BauI CACGAG 1 cut(s) 530
BbvI GCAGC 3 cut(s) 313, 445, 769
BccI CCATC 4 cut(s) 169, 480, 688, 710
BceAI ACGGC 3 cut(s) 5, 314, 581
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 1 cut(s) 651
BcnI CCSGG 2 cut(s) 227, 740
BfaI CTAG 2 cut(s) 722, 875
BfmI CTRYAG 1 cut(s) 379
BglI GCCNNNNNGGC 1 cut(s) 602
BisI GCNGC 5 cut(s) 234, 327, 459, 783, 786
BlsI GCNGC 5 cut(s) 235, 328, 460, 784, 787
Bme1390I CCNGG 3 cut(s) 227, 651, 740
Bme18I GGWCC 3 cut(s) 181, 557, 845
BmgBI CACGTC 2 cut(s) 577, 760
BmgT120I GGNCC 5 cut(s) 181, 557, 746, 747, 845
BmiI GGNNCC 2 cut(s) 747, 748
BmrFI CCNGG 3 cut(s) 227, 651, 740
Bpu10I CCTNAGC 1 cut(s) 343
BpuMI CCSGG 2 cut(s) 227, 740
BsaJI CCNNGG 2 cut(s) 225, 673
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 554, 805
Bse1I ACTGG 1 cut(s) 395
Bse21I CCTNAGG 1 cut(s) 849
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 225, 673
BseGI GGATG 1 cut(s) 699
BseLI CCNNNNNNNGG 3 cut(s) 345, 554, 805
BseMII CTCAG 2 cut(s) 257, 631
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 2 cut(s) 46, 49
BseSI GKGCMC 3 cut(s) 493, 750, 765
BseXI GCAGC 3 cut(s) 313, 445, 769
BseYI CCCAGC 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 273, 314
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 5 cut(s) 642, 654, 672, 720, 748
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 5 cut(s) 145, 216, 227, 602, 740
BslFI GGGAC 2 cut(s) 421, 809
BslI CCNNNNNNNGG 3 cut(s) 345, 554, 805
BsmFI GGGAC 2 cut(s) 421, 809
BsnI GGCC 5 cut(s) 642, 654, 672, 720, 748
Bsp120I GGGCCC 1 cut(s) 746
Bsp1286I GDGCHC 4 cut(s) 142, 493, 750, 765
Bsp143I GATC 2 cut(s) 246, 894
BspACI CCGC 7 cut(s) 5, 43, 112, 213, 234, 278, 785
BspANI GGCC 5 cut(s) 642, 654, 672, 720, 748
BspCNI CTCAG 2 cut(s) 256, 630
BspFNI CGCG 2 cut(s) 273, 314
BspLI GGNNCC 2 cut(s) 747, 748
BspPI GGATC 1 cut(s) 241
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 225, 673
BssMI GATC 2 cut(s) 246, 894
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 1 cut(s) 673
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 4 cut(s) 94, 163, 412, 685
Bst6I CTCTTC 2 cut(s) 20, 873
BstC8I GCNNGC 3 cut(s) 142, 510, 670
BstDEI CTNAG 4 cut(s) 243, 343, 617, 849
BstF5I GGATG 1 cut(s) 699
BstFNI CGCG 2 cut(s) 273, 314
BstKTI GATC 2 cut(s) 249, 897
BstMBI GATC 2 cut(s) 246, 894
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 7 cut(s) 221, 230, 488, 497, 535, 602, 769
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 3 cut(s) 225, 649, 738
BstSFI CTRYAG 1 cut(s) 379
BstSLI GKGCMC 3 cut(s) 493, 750, 765
BstUI CGCG 2 cut(s) 273, 314
BstV1I GCAGC 3 cut(s) 313, 445, 769
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
Bsu36I CCTNAGG 1 cut(s) 849
BsuRI GGCC 5 cut(s) 642, 654, 672, 720, 748
BtrI CACGTC 2 cut(s) 577, 760
BtsCI GGATG 1 cut(s) 699
Cac8I GCNNGC 3 cut(s) 142, 510, 670
Cfr13I GGNCC 5 cut(s) 181, 557, 746, 747, 845
Csp6I GTAC 1 cut(s) 435
CviAII CATG 3 cut(s) 358, 367, 713
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 4 cut(s) 243, 343, 617, 849
DpnI GATC 2 cut(s) 248, 896
DpnII GATC 2 cut(s) 246, 894
Eam1104I CTCTTC 2 cut(s) 20, 873
EarI CTCTTC 2 cut(s) 20, 873
EciI GGCGGA 3 cut(s) 32, 202, 293
Eco130I CCWWGG 1 cut(s) 673
Eco147I AGGCCT 1 cut(s) 642
Eco24I GRGCYC 2 cut(s) 142, 750
Eco47I GGWCC 3 cut(s) 181, 557, 845
Eco81I CCTNAGG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 746
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 673
EcoT38I GRGCYC 2 cut(s) 142, 750
ErhI CCWWGG 1 cut(s) 673
FaeI CATG 3 cut(s) 361, 370, 716
FaqI GGGAC 2 cut(s) 421, 809
FatI CATG 3 cut(s) 357, 366, 712
FauNDI CATATG 2 cut(s) 525, 829
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 5 cut(s) 234, 327, 459, 783, 786
FokI GGATG 1 cut(s) 706
FriOI GRGCYC 2 cut(s) 142, 750
Fsp4HI GCNGC 5 cut(s) 234, 327, 459, 783, 786
FspBI CTAG 2 cut(s) 722, 875
GluI GCNGC 5 cut(s) 234, 327, 459, 783, 786
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 5 cut(s) 642, 654, 672, 720, 748
HapII CCGG 5 cut(s) 145, 216, 227, 602, 740
Hin1II CATG 3 cut(s) 361, 370, 716
HinfI GANTC 6 cut(s) 12, 209, 239, 533, 735, 906
HpaII CCGG 5 cut(s) 145, 216, 227, 602, 740
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 5 cut(s) 11, 246, 304, 424, 808
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 4 cut(s) 209, 268, 578, 761
HpyAV CCTTC 3 cut(s) 49, 548, 623
HpyCH4III ACNGT 4 cut(s) 94, 163, 412, 685
HpyCH4IV ACGT 2 cut(s) 576, 759
HpyCH4V TGCA 2 cut(s) 529, 727
HpyF10VI GCNNNNNNNGC 7 cut(s) 221, 230, 488, 497, 535, 602, 769
HpyF3I CTNAG 4 cut(s) 243, 343, 617, 849
HpySE526I ACGT 2 cut(s) 576, 759
Hsp92II CATG 3 cut(s) 361, 370, 716
Kzo9I GATC 2 cut(s) 246, 894
LmnI GCTCC 1 cut(s) 801
Lsp1109I GCAGC 3 cut(s) 313, 445, 769
MaeI CTAG 2 cut(s) 722, 875
MaeII ACGT 2 cut(s) 576, 759
MaeIII GTNAC 1 cut(s) 22
MalI GATC 2 cut(s) 248, 896
MboI GATC 2 cut(s) 246, 894
MboII GAAGA 5 cut(s) 7, 307, 622, 872, 890
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 4 cut(s) 142, 493, 750, 765
MluCI AATT 4 cut(s) 252, 258, 473, 678
MlyI GAGTC 2 cut(s) 6, 542
MmeI TCCRAC 1 cut(s) 864
MseI TTAA 1 cut(s) 570
MspI CCGG 5 cut(s) 145, 216, 227, 602, 740
MspR9I CCNGG 3 cut(s) 227, 651, 740
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 2 cut(s) 273, 314
MwoI GCNNNNNNNGC 7 cut(s) 221, 230, 488, 497, 535, 602, 769
NciI CCSGG 2 cut(s) 227, 740
NdeI CATATG 2 cut(s) 525, 829
NdeII GATC 2 cut(s) 246, 894
NlaIII CATG 3 cut(s) 361, 370, 716
NlaIV GGNNCC 2 cut(s) 747, 748
NmuCI GTSAC 1 cut(s) 22
PceI AGGCCT 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PfeI GAWTC 4 cut(s) 209, 239, 735, 906
PkrI GCNGC 5 cut(s) 235, 328, 460, 784, 787
PleI GAGTC 2 cut(s) 6, 541
PpsI GAGTC 2 cut(s) 6, 541
PsiI TTATAA 1 cut(s) 928
Psp6I CCWGG 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 2 cut(s) 747, 748
PspOMI GGGCCC 1 cut(s) 746
PspPI GGNCC 5 cut(s) 181, 557, 746, 747, 845
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 5 cut(s) 234, 327, 459, 783, 786
Sau3AI GATC 2 cut(s) 246, 894
Sau96I GGNCC 5 cut(s) 181, 557, 746, 747, 845
SchI GAGTC 2 cut(s) 6, 542
ScrFI CCNGG 3 cut(s) 227, 651, 740
SduI GDGCHC 4 cut(s) 142, 493, 750, 765
SfcI CTRYAG 1 cut(s) 379
SinI GGWCC 3 cut(s) 181, 557, 845
Sse9I AATT 4 cut(s) 252, 258, 473, 678
SseBI AGGCCT 1 cut(s) 642
SsiI CCGC 7 cut(s) 5, 43, 112, 213, 234, 278, 785
SspMI CTAG 2 cut(s) 722, 875
StuI AGGCCT 1 cut(s) 642
StyD4I CCNGG 3 cut(s) 225, 649, 738
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 4 cut(s) 94, 163, 412, 685
TaiI ACGT 2 cut(s) 579, 762
TaqI TCGA 5 cut(s) 204, 290, 564, 816, 858
TasI AATT 4 cut(s) 252, 258, 473, 678
TauI GCSGC 2 cut(s) 236, 788
TfiI GAWTC 4 cut(s) 209, 239, 735, 906
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 3 cut(s) 326, 458, 782
Tsp45I GTSAC 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 252, 549
VpaK11BI GGWCC 3 cut(s) 181, 557, 845
XapI RAATTY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 2 cut(s) 722, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.