pycom07g25370

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001923
Physical Location & Seq
Reverse (-)
25703570 .. 25705958
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25370.1

Sequence Viewer

Length: 930 bp
ATGGCGGTCGGACTCTTCGCCGTCACAACCCCCACCCCCTTCCGCCTAATCCACTCCTCCTCCTACCACCTCCCAAAACCCAACCGCACCACCGTCTTCTTCCCAAAACTCCGCCACCAGAAGTCAGCACTCCACAGGGCTCGCCGGAAACTCTGTTTCACCGTCTATGTAGTGATGGAGGACCAAAAACAGATAACCAACCTCGACAATTCCGCCGGAAAATCCCCGGAAGCCGCCGATTCTCAGATCCCAATTGTAATTCCGTCGGTTCGCGTGGCGGAGAGGCTGTCGAGGAAGAAATCGGAGAGGTTCGCGTATCTTGTCGCTGCCGTGATGTCCAGCTTAGGGATTACTTCCATGGCTGTCATGGCTGTTTACTACAGATTTTACTGGCAAATGGAGGGTGGGACTGTGCCATTGTCTGAAATGCTGGGTACATTTGCTCTATCTGTTGGTGCTGCTGTGGGAATGGAGTTTTGGGCAAGATGGGCACATAAGGCTCTTTGGCACGCTTCCTTATGGCATATGCACGAGTCGCACCACCGACCCAGAGAAGGTCCGTTCGAGCTTAACGACGTGTTTGCCATAATCAACGCCGTTCCGGCAATAGCCCTTCTCAGTTATGGTTTCTTCCACAAAGGCCTTGTTCCTGGCCTTTGTTTTGGTGCTGGCCTTGGAATTACGGTGTTTGGGATGGCGTATATGTTTGTCCACGATGGCCTAGTGCATAAAAGATTCCCAGTGGGGCCAATAGCCGATGTGCCCTATTTGCGAAAGGTTGCTGCAGCTCATCAGCTCCACCACTCGGAGAAGTTCGAGGGTGTTCCATATGGTTTGTTTCTTGGACCTAAGGAACTCGAAGAAGTGGGAGGACTAGAAGAGTTGGAAAAGGAGATCGATAGGAGAATCAAAGCATACAAGGGCTTATAA

Protein Analysis

310

Amino Acids

34.6

Weight (kDa)

9.48

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 153 - 281 2e-14 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 928
AccII CGCG 2 cut(s) 273, 314
AciI CCGC 7 cut(s) 5, 43, 85, 112, 213, 234, 278
AclWI GGATC 1 cut(s) 241
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 3 cut(s) 345, 554, 805
AflIII ACRYGT 1 cut(s) 576
AjiI CACGTC 1 cut(s) 577
AjnI CCWGG 1 cut(s) 649
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AluBI AGCT 4 cut(s) 342, 568, 788, 796
AluI AGCT 4 cut(s) 342, 568, 788, 796
AlwI GGATC 1 cut(s) 241
AoxI GGCC 5 cut(s) 640, 652, 670, 718, 746
ApeKI GCWGC 4 cut(s) 326, 458, 782, 785
AspS9I GGNCC 4 cut(s) 181, 557, 746, 845
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 3 cut(s) 181, 557, 845
AxyI CCTNAGG 1 cut(s) 849
BaeGI GKGCMC 2 cut(s) 493, 765
BanII GRGCYC 1 cut(s) 142
BauI CACGAG 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 4 cut(s) 313, 445, 769, 797
BccI CCATC 4 cut(s) 169, 480, 688, 710
BceAI ACGGC 3 cut(s) 5, 314, 581
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 1 cut(s) 651
BcnI CCSGG 1 cut(s) 227
BfaI CTAG 2 cut(s) 722, 875
BfmI CTRYAG 2 cut(s) 379, 783
BglI GCCNNNNNGGC 1 cut(s) 602
BisI GCNGC 5 cut(s) 234, 327, 459, 783, 786
BlsI GCNGC 5 cut(s) 235, 328, 460, 784, 787
Bme1390I CCNGG 2 cut(s) 227, 651
Bme18I GGWCC 3 cut(s) 181, 557, 845
BmgBI CACGTC 1 cut(s) 577
BmgT120I GGNCC 4 cut(s) 181, 557, 746, 845
BmiI GGNNCC 1 cut(s) 747
BmrFI CCNGG 2 cut(s) 227, 651
BmrI ACTGGG 1 cut(s) 734
BmuI ACTGGG 1 cut(s) 734
BpiI GAAGAC 1 cut(s) 88
Bpu10I CCTNAGC 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 897
BsaJI CCNNGG 3 cut(s) 225, 357, 673
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 554, 805
Bse1I ACTGG 2 cut(s) 395, 740
Bse21I CCTNAGG 1 cut(s) 849
BseBI CCWGG 1 cut(s) 651
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 3 cut(s) 225, 357, 673
BseGI GGATG 1 cut(s) 699
BseLI CCNNNNNNNGG 3 cut(s) 345, 554, 805
BseMII CTCAG 2 cut(s) 257, 631
BseNI ACTGG 2 cut(s) 395, 740
BseRI GAGGAG 2 cut(s) 46, 49
BseSI GKGCMC 2 cut(s) 493, 765
BseXI GCAGC 4 cut(s) 313, 445, 769, 797
BseYI CCCAGC 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 273, 314
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 5 cut(s) 642, 654, 672, 720, 748
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 4 cut(s) 145, 216, 227, 602
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 3 cut(s) 345, 554, 805
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 5 cut(s) 642, 654, 672, 720, 748
Bsp1286I GDGCHC 3 cut(s) 142, 493, 765
Bsp143I GATC 2 cut(s) 246, 894
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 7 cut(s) 5, 43, 85, 112, 213, 234, 278
BspANI GGCC 5 cut(s) 642, 654, 672, 720, 748
BspCNI CTCAG 2 cut(s) 256, 630
BspDI ATCGAT 1 cut(s) 897
BspFNI CGCG 2 cut(s) 273, 314
BspLI GGNNCC 1 cut(s) 747
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 1 cut(s) 241
BsrI ACTGG 2 cut(s) 395, 740
BssECI CCNNGG 3 cut(s) 225, 357, 673
BssMI GATC 2 cut(s) 246, 894
BssSI CACGAG 1 cut(s) 530
BssT1I CCWWGG 2 cut(s) 357, 673
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 4 cut(s) 94, 163, 412, 685
Bst6I CTCTTC 2 cut(s) 20, 873
BstC8I GCNNGC 3 cut(s) 142, 510, 670
BstDEI CTNAG 4 cut(s) 243, 343, 617, 849
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 699
BstFNI CGCG 2 cut(s) 273, 314
BstKTI GATC 2 cut(s) 249, 897
BstMBI GATC 2 cut(s) 246, 894
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 6 cut(s) 368, 488, 497, 535, 602, 769
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 2 cut(s) 225, 649
BstSFI CTRYAG 2 cut(s) 379, 783
BstSLI GKGCMC 2 cut(s) 493, 765
BstUI CGCG 2 cut(s) 273, 314
BstV1I GCAGC 4 cut(s) 313, 445, 769, 797
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
Bsu15I ATCGAT 1 cut(s) 897
Bsu36I CCTNAGG 1 cut(s) 849
BsuRI GGCC 5 cut(s) 642, 654, 672, 720, 748
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 577
BtsCI GGATG 1 cut(s) 699
BtsIMutI CAGTG 1 cut(s) 747
Cac8I GCNNGC 3 cut(s) 142, 510, 670
Cfr13I GGNCC 4 cut(s) 181, 557, 746, 845
ClaI ATCGAT 1 cut(s) 897
Csp6I GTAC 1 cut(s) 435
CviAII CATG 2 cut(s) 358, 367
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 4 cut(s) 243, 343, 617, 849
DpnI GATC 2 cut(s) 248, 896
DpnII GATC 2 cut(s) 246, 894
Eam1104I CTCTTC 2 cut(s) 20, 873
EarI CTCTTC 2 cut(s) 20, 873
EciI GGCGGA 4 cut(s) 32, 101, 202, 293
Eco130I CCWWGG 2 cut(s) 357, 673
Eco147I AGGCCT 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 3 cut(s) 181, 557, 845
Eco81I CCTNAGG 1 cut(s) 849
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 2 cut(s) 357, 673
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 357, 673
FaeI CATG 2 cut(s) 361, 370
FaqI GGGAC 1 cut(s) 421
FatI CATG 2 cut(s) 357, 366
FauNDI CATATG 2 cut(s) 525, 829
Fnu4HI GCNGC 5 cut(s) 234, 327, 459, 783, 786
FokI GGATG 1 cut(s) 706
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 5 cut(s) 234, 327, 459, 783, 786
FspBI CTAG 2 cut(s) 722, 875
GluI GCNGC 5 cut(s) 234, 327, 459, 783, 786
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 5 cut(s) 642, 654, 672, 720, 748
HapII CCGG 4 cut(s) 145, 216, 227, 602
Hin1II CATG 2 cut(s) 361, 370
HinfI GANTC 5 cut(s) 12, 239, 533, 735, 906
HpaII CCGG 4 cut(s) 145, 216, 227, 602
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 2 cut(s) 376, 712
Hpy188I TCNGA 5 cut(s) 11, 246, 304, 424, 808
Hpy8I GTNNAC 2 cut(s) 376, 712
Hpy99I CGWCG 2 cut(s) 268, 578
HpyAV CCTTC 3 cut(s) 49, 548, 623
HpyCH4III ACNGT 4 cut(s) 94, 163, 412, 685
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 3 cut(s) 529, 727, 785
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 488, 497, 535, 602, 769
HpyF3I CTNAG 4 cut(s) 243, 343, 617, 849
HpySE526I ACGT 1 cut(s) 576
Hsp92II CATG 2 cut(s) 361, 370
Kzo9I GATC 2 cut(s) 246, 894
LmnI GCTCC 1 cut(s) 801
Lsp1109I GCAGC 4 cut(s) 313, 445, 769, 797
MaeI CTAG 2 cut(s) 722, 875
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 1 cut(s) 22
MalI GATC 2 cut(s) 248, 896
MboI GATC 2 cut(s) 246, 894
MboII GAAGA 7 cut(s) 7, 88, 91, 307, 622, 872, 890
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 3 cut(s) 142, 493, 765
MluCI AATT 4 cut(s) 208, 252, 258, 678
MlyI GAGTC 2 cut(s) 6, 542
MmeI TCCRAC 1 cut(s) 864
MseI TTAA 1 cut(s) 570
MspI CCGG 4 cut(s) 145, 216, 227, 602
MspR9I CCNGG 2 cut(s) 227, 651
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 2 cut(s) 273, 314
MwoI GCNNNNNNNGC 6 cut(s) 368, 488, 497, 535, 602, 769
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 525, 829
NdeII GATC 2 cut(s) 246, 894
NlaIII CATG 2 cut(s) 361, 370
NlaIV GGNNCC 1 cut(s) 747
NmuCI GTSAC 1 cut(s) 22
PceI AGGCCT 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PfeI GAWTC 3 cut(s) 239, 735, 906
PkrI GCNGC 5 cut(s) 235, 328, 460, 784, 787
PleI GAGTC 2 cut(s) 6, 541
PpsI GAGTC 2 cut(s) 6, 541
PsiI TTATAA 1 cut(s) 928
Psp6I CCWGG 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 1 cut(s) 747
PspPI GGNCC 4 cut(s) 181, 557, 746, 845
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 5 cut(s) 234, 327, 459, 783, 786
Sau3AI GATC 2 cut(s) 246, 894
Sau96I GGNCC 4 cut(s) 181, 557, 746, 845
SchI GAGTC 2 cut(s) 6, 542
ScrFI CCNGG 2 cut(s) 227, 651
SduI GDGCHC 3 cut(s) 142, 493, 765
SfcI CTRYAG 2 cut(s) 379, 783
SinI GGWCC 3 cut(s) 181, 557, 845
Sse9I AATT 4 cut(s) 208, 252, 258, 678
SseBI AGGCCT 1 cut(s) 642
SsiI CCGC 7 cut(s) 5, 43, 85, 112, 213, 234, 278
SspMI CTAG 2 cut(s) 722, 875
StuI AGGCCT 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 225, 649
StyI CCWWGG 2 cut(s) 357, 673
TaaI ACNGT 4 cut(s) 94, 163, 412, 685
TaiI ACGT 1 cut(s) 579
TaqI TCGA 6 cut(s) 204, 290, 564, 816, 858, 897
TasI AATT 4 cut(s) 208, 252, 258, 678
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 3 cut(s) 239, 735, 906
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 747
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 4 cut(s) 326, 458, 782, 785
Tsp45I GTSAC 1 cut(s) 22
TspGWI ACGGA 2 cut(s) 252, 549
TspRI CASTG 1 cut(s) 747
VpaK11BI GGWCC 3 cut(s) 181, 557, 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XspI CTAG 2 cut(s) 722, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.